CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Junli Wang,Yuanqiang Sun,Jingyu Hu,Yifei Guo,Lingbo Qu,Zhaohui Li
Hepatocellular carcinoma (HCC) is a metabolic disease with high mortality caused by abnormal deposition of lipid droplets (LDs). The number and distribution of LDs can effectively reflect the severity of HCC. Additionally, polarity change is also an important indicator for monitoring HCC. Therefore, using fluorescence imaging technology to accurately identify LDs is helpful for HCC diagnosis and treatment evaluation. To date, the study that can simultaneously monitor the polarity and spatiotemporal characteristics of LDs to diagnose HCC has not been reported yet. Herein, a novel LD-targeting near-infrared carbon dots (CDs) with dual-report function of determining the LDs polarity as well as presenting their spatial characteristics was developed. The high lipophilicity and solvatochromic effect of CDs endows it with the ability to image LDs with high-fidelity, so it can not only report the spatiotemporal characteristics of LDs including the number, size, and aggregation degree, but also determine their polarity variations. Therefore, the high-contrast fluorescence diagnosis of HCC was successfully realized by using dual-function CDs. Meanwhile, the CDs were further utilized to evaluate the efficacy of anticancer drugs with satisfactory performance. Overall, this CD-based LDs probe may provide a new perspective for the diagnosing of LDs-related diseases and screening of anticancer drugs.
研究领域:
生物医学
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肿瘤学
·
分子影像学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Liying Jin,Chuanfeng Zhao,Xiaosong Wang,Qian Zhang,Yuliang Jiang,Jian Shen
The excessive use of pyrophosphate (PPi) anions as additives poses a serious threat to human health and the environment. Considering the current status of PPi probes, the development of metal-free auxiliary PPi probes has important applications. In this study, a novel near-infrared nitrogen and sulfur co-doped carbon dots (N,S-CDs) were prepared. The average particle size of N,S-CDs was 2.25 ± 0.32 nm with average height was 3.05 nm. The probe N,S-CDs showed a special response to PPi, and a good linear relationship was obtained with PPi concentrations ranging from 0 to 1 μM, with the limit of detection being 0.22 nM. Tap water and milk were used for practical inspection, and ideal experimental results were acquired. In addition, the probe N,S-CDs also showed good results in biological systems, such as cell and zebrafish experiments.
研究领域:
分析化学
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环境科学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Yunting Qu,Danni Li,Jinrui Liu,Fangkai Du,Xuecai Tan,Yuzhu Zhou,Shaogang Liu,Weihua Xu
In this work, an eco-friendly unique approach was used to synthesize near-infrared carbon dots (NIR-CDs) from Magnolia denudata leaves. The as-prepared NIR-CDs showed a stable near-infrared emission with peak at about 678 nm and narrow full width at half maximum (FWHM), which was utilized as a fluorophore in a fluorescent nanoprobe after modification with Pluronic F-127 for detection of palladium ion (Pd 2+ ). The resulting nanoprobe diaplayed admirable performances for detecting Pd 2+ including high selectivity, excellent water solubility, and stability. The fluorescence intensity of the nanoprobe was selectivity quenched by Pd 2+ within the scope of 0–200 μM. In addition, the limit of detection (LOD) was 85.3 nM. Moreover, the constructed nanoprobe had an approving assay performance in real water samples. Finally, the nanoprobe demonstrated low toxicity and could be utilized to monitor Pd 2+ in Li-7 cells.
研究领域:
材料科学
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分析化学
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生物医学工程
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xuyuan Sun,Hao Liu,Zhengyuan Dai,Ying Wang,Li Li,Yaping Ding
A molecularly imprinted fluorescent aptasensor was designed for selective detection of quinine (Qn) based on dual functional monomers. In the sol-gel polymerization of molecularly imprinted polymers (MIPs), 3-aminopropyl triethoxysilane (APTES) and quinine aptamer (Apt) were employed as dual functional monomers, and Qn was the template molecule. Near-infrared carbon dots (RCDs) were used as fluorescence signal probe, and effectively avoided the interference of fluorescence emitted by Qn. Due to the diverse interaction among APTES, Apt and Qn, the near-infrared fluorescent molecularly imprinted aptasensor (RCDs-Apt@MIPs) exhibited higher specificity towards Qn than the sensor with APTES or Apt as a single functional monomer. The sensor had a wide linear range of 5.0–110.0 μM with a low detection limit of 1.7 μM. Satisfactory recovery between 93.4 % and 104.0 % with relative standard deviation from 1.30 % to 3.60 % in drinks, which showed great potential that this method can be used to detect Qn in food.
研究领域:
分析化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2021
·Vol. 19
·期 1
·1-14
·
IF 10.435
Wang Yuhui,Xie Zhuomi,Wang Xiuhua,Peng Xin,Zheng Jianping
Background Fluorescent carbon-dots (CDs) with multifaceted advantages have provided hope for improvement of crop growth. Near infrared (NIR) CDs would be more competitive and promising than short-wavelength emissive CDs, which are not directly utilized by chloroplast. The molecular targets and underlying mechanism of these stimulative effects are rarely mentioned. Results NIR-CDs with good mono-dispersity and hydrophily were easily prepared by a one-step microwave-assisted carbonization manner, which showed obvious UV absorptive and far-red emissive properties. The chloroplast-CDs complexes could accelerate the electron transfer from photosystem II (PS II) to photosystem I (PS I). NIR-CDs exhibited a concentration-dependent promotion effect on N. benthamiana growth by strengthening photosynthesis. We firstly demonstrated that potential mechanisms behind the photosynthesis-stimulating activity might be related to up-regulated expression of the photosynthesis and chloroplast synthesis related genes, among which PsbP and PsiK genes are the key regulators. Conclusion These results illustrated that NIR-CDs showed great potential in the applications to increase crop yields through ultraviolet light harvesting and elevated photosynthesis efficiency. This work would provide a theoretical basis for the understanding and applications of the luminescent nanomaterials (not limited to CDs) in the sunlight conversion-related sustainable agriculture. Graphic abstract
研究领域:
农业
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植物生理学
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分子生物学
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纳米材料科学
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2022
·Vol. 11
·期 12
·2102759
·
IF 11.092
Yulong Bai,Jingjin Zhao,Liangliang Zhang,Shulong Wang,Jing Hua,Shulin Zhao,Hong Liang
Tumor microenvironment (TME)-activated cancer imaging and therapy is a key to achieving accurate diagnosis and treatment of cancer and reducing the side effects. Herein, smart near-infrared carbon dot-metal organic framework MIL-100 (Fe) assemblies are constructed to achieve TME-activated cancer imaging and chemodynamic-photothermal combined therapy. First, a near-infrared emission carbon dot (RCDs) is developed using glutathione (GSH) as the precursor. Then, the RCDs@MIL-100 self-assemblies are obtained using RCDs, FeCl 3 , and trimesic acid solutions as raw materials. After the RCDs@MIL-100 enters the TME, a high concentration of GSH reduces Fe 3+ to Fe 2+ and drains the GSH, triggering the collapse of RCDs@MIL-100 skeleton and the release of RCDs and Fe 2+ , at which time the RCDs fluorescence is restored and in an “on” state to illuminate the tumor cells, which achieved cancer imaging. The released Fe 2+ reacts with H 2 O 2 in the TME to form highly reactive hydroxyl radicals (•OH) by Fenton reaction, which achieves the chemodynamic therapy of tumors. Thus, efficient synergistic chemodynamic-photothermal dual mode therapy is achieved under fluorescence imaging guidance with TME response.
研究领域:
生物医学工程
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癌症研究
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材料科学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 4
·1-9
·
IF 6.408
Cui Hanyue,Yang Jing,Lu Huan,Li Li,Zhu Xiaoli,Ding Yaping
A label-free fluorescence sensor based on near-infrared carbon dots (NIR-CDs) and aptamer is described for the highly sensitive and selective detection of ciprofloxacin (CIP). NIR-CDs were synthesized from polyethyleneimine and reduced glutathione by one-step hydrothermal method. The electrostatic interaction between the positively charged carbon dots and the negatively charged aptamer resulted in fluorescence quenching. After the addition of CIP, the specific binding between CIP aptamer and CIP was stronger, resulting in fluorescence recovery. Under the optimal experimental conditions, the recovered fluorescence intensity has a linear relationship with the concentration of CIP in the range 0.5–800 ng/mL, and the detection limit is 0.167 ng/mL. The prepared carbon dots have excellent optical properties and biocompatibility, and due to their emission characteristics in the near-infrared window, they can be used for biological imaging, which has also been confirmed in the experiment. The feasibility of the label-free fluorescence sensor for the detection of CIP is also proved by confocal fluorescence imaging. The detection results of CIP determination in milk by this sensor are satisfactory, indicating that the developed sensor has great application potential. Graphical abstract
研究领域:
分析化学
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生物技术
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生物化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL SCIENCES
·
2023
·Vol. 39
·期 7
·1097-1106
·
IF 1.6
Xie Wenfei,Liu Jinrui,Qu Yunting,Du Fangkai
In this work, a convenient ratiometric fluorescent platform was designed to measure organophosphorus pesticides (OPs) based on acetylcholinesterase (AChE), acetylthiocholine (ATCh), manganese dioxide nanosheets (MnO 2 ), near-infrared carbon dots (RCDs) and o-phenylenediamine (OPD). In this platform, a direct oxidation of OPD by MnO 2 generated the luminescent product 2,3-diaminophenolazine (DAP) through intrinsic oxidase activity, while RCDs served as a fluorescent reference indicator. In the presence of AChE and ATCh, the enzymatic hydrolysate thiocholine (TCh) would reduce MnO 2 nanosheets to Mn 2+ , leading to the quenching of DAP fluorescence. On the other hand, OPs can inhibit the catabolism of ATCh by AChE thus acting as a recognizer of OPs. According to these reactions, OPs were quantitatively analyzed by the intensity ratio of fluorescence emitted from RCDs and DAP ( F 560 / F 676 ). The constructed platform can detect OPs with the range of 0.2–0.6 μM with a detection limit of 4.3 nM. Graphical abstract Figure A ratiometric fluorescent probe based on carbon dots was obtained and using it to determine the concentration of organophosphorus pesticides
研究领域:
分析化学
·
环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Qi Xiao,Huishan Cao,Xincong Tu,Chunyan Pan,Yi Fang,Shan Huang
In recent years, the exceptional biocatalytic properties of glucose oxidase (GOx) have spurred the development of various GOx-functionalized nanocatalysts for cancer diagnosis and treatment. Carbon dots, renowned for their excellent biocompatibility and distinctive fluorescence properties, effectively incorporate GOx. Given the paramount importance of GOx's enzymatic activity in therapeutic efficacy, this study conducts a thorough exploration of the molecular-level binding dynamics between GOx and near-infrared carbon dots (NIR-CDs). Utilizing various spectrometric and molecular simulation techniques, we reveal that NIR-CDs form a ground-state complex with GOx primarily via hydrogen bonds and van der Waals forces, interacting directly with amino acid residues in GOx's active site. This binding leads to conformational change and reduces thermal stability of GOx, slightly inhibiting its enzymatic activity and demonstrating a competitive inhibition effect. In vitro experiments demonstrate that NIR-CDs attenuate the GOx's capacity to produce H 2 O 2 in HeLa cells, mitigating enzyme-induced cytotoxicity and cellular damage. This comprehensive elucidation of the intricate binding mechanisms between NIR-CDs and GOx provides critical insights for the design of NIR-CD-based nanotherapeutic platforms to augment cancer therapy. Such advancements lay the groundwork for innovative and efficacious cancer treatment strategies.
研究领域:
纳米技术
·
生物化学
·
分子生物学
·
癌症研究
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）