MOLECULAR & CELLULAR PROTEOMICS
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2024
·Vol. 23
·期 6
·
IF 6.1
Jiao Qi,Hao Li,Yu Du,Yun Liu,Wenwen He,Jiaqi Meng,Ling Wei,Keke Zhang,Yi Lu,Xiangjia Zhu
High myopia is a leading cause of blindness worldwide, among which pathologic myopia, characterized by typical myopic macular degeneration, is the most detrimental. However, its pathogenesis remains largely unknown. Here, using a HuProt array, we first initiated a serological autoantibody profiling of high myopia and identified 18 potential autoantibodies, of which anti-LIMS1 autoantibody was validated by a customized focused microarray. Further subgroup analysis revealed its actual relevance to pathologic myopia, rather than simple high myopia without myopic macular degeneration. Mechanistically, anti-LIMS1 autoantibody predominantly belonged to IgG1/IgG2/IgG3 subclasses. Serum IgG obtained from patients with pathologic myopia could disrupt the barrier function of retinal pigment epithelial cells via cytoskeleton disorganization and tight junction component reduction, and also trigger a pro-inflammatory mediator cascade in retinal pigment epithelial cells, which were all attenuated by depletion of anti-LIMS1 autoantibody. Together, these data uncover a previously unrecognized autoimmune etiology of myopic macular degeneration in pathologic myopia.
研究领域:
眼科
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免疫学
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病理学
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DOI
PubMed
高影响因子
Translational Pediatrics
·
2021
·Vol. 10
·期 4
·860
·
IF 2.488
Yang Shen,Jing Zhao,Ling Sun,Li Zeng,Zhi Chen,Mi Tian,Xingtao Zhou
Background The syndrome of monocular myelinated retinal nerve fibers (MRNF), myopia and amblyopia threatens visual development of infants and young children. The efficacy of part-time occlusion therapy remains controversial, and the long-term prognosis of the syndrome remains unclear. Methods Ten children (4.40±2.22 years, 4 boys and 6 girls) with monocular MRNF, myopia and amblyopia were recruited. Both the affected eyes (treatment group) and the fellow eyes (control group) underwent routine ophthalmic examinations, including AL, cycloplegic refraction, best-spectacles-corrected distance visual acuity (BSCDVA), cover test, simultaneous perception, corneal curvature, anterior segment and fundus examinations. Refractive error was corrected by either spectacles or rigid gas permeable (RGP) contact lenses. Part-time occlusion therapy was employed for amblyopia treatment. The retinal characteristics were examined only on the final visit with a Fourier-domain optical coherence tomography (FD-OCT). The speed of myopia progression and axial elongation were calculated. Statistical analysis was performed using SPSS 20 software. Cut-off P values were 0.05. Results On the final visit, six children had normal simultaneous perception but three had strabismus. The mean AL and spherical equivalent (SE) values of the affected eyes were 28.05±1.59 mm and −12.60±4.20 D, respectively, while those of the fellow eyes were 23.67±1.13 mm and −0.66±1.99 D, respectively. AL and SE were significantly different over time and between groups (P<0.0001), but no time*group interaction effect (P>0.05) was observed. The differences in the corneal curvature, mean speed of myopia progression, axial elongation, foveal thickness and parafoveal thickness between the two groups were not significant (P>0.05). However, the perifoveal thickness in the affected eyes was significantly higher than that of the control eyes (P=0.047). In the treatment group, the occlusion dosage positively correlated with the final BSCDVA (R=0.764, P=0.016) but did not correlate with the speed of myopia progression or axial elongation (both P values >0.05). Conclusions The affected eyes have longer AL, severer myopia and thicker perifoveal retina when compared with the fellow eyes. Both the affected and the fellow eyes have similar speed of myopia progression as well as that of axial elongation.
研究领域:
眼科
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小儿眼科学
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屈光学
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视觉发育
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影像学
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临床研究
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Baijun Li,Meiqing Guo,Xiaojun Wang,Liyong Qiao,Hui Song,Zhiqiang Li
The effectiveness of hard orthokeratology (OK) lenses in controlling myopia has been confirmed in clinical practice. However, there exist defects including wear discomfort, limited regulation of mechanical properties and geometry, and effectiveness inconsistency with the expected efficacy. Herein, we analyze the relationship between the mechanical properties of OK lenses and the vertex displacement of the human cornea through the finite element model. It is noted that the expected corneal deformation of 15–20 μm can be achieved by soft OK lenses with an elasticity modulus above 9 MPa, which is far lower than the 2.7 GPa of commercial OK lenses. No obvious displacement difference occurred when the elasticity modulus of the OK lenses exceeded 200 MPa. Thus, we propose the hypothesis of soft OK lenses based on hydrogel materials for controlling myopia. The soft OK lenses are fabricated by a two-step 3D printing technology using a multicomponent bioink containing methyl methacrylate, hydroxyethyl methacrylate, polyethylene glycol diacrylate, and 3-(trimethoxysilyl)propyl methacrylate (KH-570). The printed OK lenses possess comfort wear, high light transmittance, smooth surface, regulated elasticity modulus (from 33 to 535 MPa) and geometry, and excellent biocompatibility. Additionally, the effectiveness of soft OK lenses is confirmed through a porcine corneal model with obvious corneal deformation comparable to that of the commercial ones. This study demonstrates the feasibility of soft OK lenses for controlling myopia and provides support for the design and fabrication of soft OK lenses for comfortable wear.
研究领域:
生物医学工程
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眼科研究
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材料科学
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PubMed
高影响因子
Metabolites
·
2022
·Vol. 12
·期 12
·1220
·
IF 5.581
Tiansheng Chou,Xiaosheng Huang,Jiawei Liu,Xinhua Liu,Kun Zeng,Zonghui Yan,Shaoyi Mei,Liangnan Sun,Wenqun Xi,Jinglan Ni,Jin Zi,Jun Zhao,Siqi Liu
The quantitative level of steroid hormones (SHs) in some body fluids have been accepted for clinical diagnosis, whereas their distribution in aqueous humor (AH) is unknown yet. Herein, a profiling study was conducted with a total of 171 AH and 107 plasma samples using liquid chromatography coupled with tandem mass spectrometry (LC MS/MS). For the first time, six kinds of SHs in AH were quantitatively estimated, and their abundances were ranked at cortisol (F), corticosterone (COR), androstenedione (A2), and 11-deoxycortisol (11DOC). The corresponding abundance of all SHs in AH was significantly lower than those in plasma, while there was a lack of a proportional relationship with the abundance of plasma SHs. Dehydroepiandrosterone sulfate, the most abundant plasma SH, was undetectable in AH, implying that the blood–aqueous barrier might specifically block its transferral. Axial myopia generally results from many factors throughout the entire eye from tissues and molecules; furthermore, the correlation of AH SHs and axial myopia was assessed to look for their indication in such myopia. The panel with five kinds of AH SHs (F, COR, CORT, ALD and A2) was functional as a discriminator for axial myopia and control. The abundance of SHs, therefore, has a specific distribution in AH and can potentially contribute to axial myopia.Keywords:steroid hormone;aqueous humor;blood–aqueous barrier (BAB);axial length (AL)
研究领域:
眼科学
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内分泌学
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PubMed
高影响因子
3D Printing and Additive Manufacturing
·
2025
·
IF 2.1
Feilong Jiang,Zhuo Zhao,Chuangjia Zhao,Liangjie Xie,Meiqi Wang,Yulu Cheng,Xubao Wang
Facing the serious problem of rapidly increasing myopia rate, the demand for eyeglasses is increasing, while the traditional manufacturing methods for eyeglass frames and lenses are long, slow, and expensive. In this article, we propose to fabricate myopia glasses by 3D printing method, and the frames and lenses are produced by digital light processing and stereo lithography appearance methods, respectively. Through the tensile and elongation at break test, it shows that the mechanical properties of the 3D printed frames are good, and the mechanical collision simulation results show that it does not affect the normal use under the impact of large speeds, which shows a high degree of uniformity with the mechanical experiments; through the pressure test at different positions after wearing, it verifies the wearing comfort of the myopic frames; after polishing the printed myopic lenses, through the optical experiments, it shows that the transmission rate of the myopic lens is comparable to that of the traditional glasses. The transmission rate has been more similar to the traditional glasses; measurement of the refraction of the printed myopic lenses compared with the theory, the error is within the acceptable range. Through the wearing test, it shows that the 3D-printed myopic lenses have a certain corrective effect on visual acuity.
研究领域:
生物医学工程
·
材料科学
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Jiaxin Zhang,Mengxia Lu,Xin Cai,Peter Müller-Buschbaum,Qi Zhong
A wearable detector to monitor visible light intensity is realized by the restrained photochromism of a hydrogel composite containing light-responsive spiropyran with hydroxyl groups (SPOH). When exposed to visible light, the SPOH experiences a ring-opening to a ring-closed transition accompanied by discoloration from red to yellow. Unlike in the solution, the photochromism/discoloration rate is strongly correlated to the cross-linking points. By reducing the amount of cross-linker from 40 to 5 mg, the photochromism rate of SPOH is 300% faster. Inspired by the Chinese Jade Loong from Hongshan, the hydrogel composite is shaped into a Loong to monitor the light intensity. By increasing the amount of cross-linker in the head, body, and tail, the photochromism/discoloration rate sequentially turns slower from one region to the other. Higher light intensity is required to realize the discoloration in the hydrogel composite containing a larger amount of the cross-linker. Because the initial colors are identical, the light intensity can be easily traced by checking the discoloration of these pieces containing different amounts of cross-linker. Based on this unique and reversible photochromic capability, the present hydrogel composite can be used for monitoring the visible light intensity to prevent myopia, especially for children and students.
研究领域:
材料科学
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生物医学工程
·
光学
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产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
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2025
·
IF 9.6
Yueze Hong,Jianyu Xin,Peng Wang,Yanhong Song,Xiangling Fan,Li Yang,Gaoyang Guo,Daihua Fu,Yan Dai,Fanjun Zhang,Yunbing Wang
Myopia has become a significant public health problem in recent decades, resulting in a profound public health and financial burden. The phakic intraocular lens (PIOL) utilized in myopia intraocular refractive surgery is constantly facing challenges in terms of uveal biocompatibility. Inspired by the “sandwich theory”, this study proposes the hypothesis that fibronectin (FN) can improve biocompatibility, and then creatively constructs a selective in-situ trap FN strategy. Specifically, PIOL surfaces with amide bonds covalently linking collagen were prepared, where selectivity was achieved by specific binding of collagen to FN. The obtained collagen modified material reduces immune response by reducing M1 polarization of macrophages, and its functionality and safety have been verified in vitro and in vivo. The grafting of collagen on the PIOL surface was able to occupy adsorption sites and inhibit the non-specific adsorption of other proteins while mimicking the extracellular matrix (ECM) microenvironment, further reducing the foreign body rejection. Overall, this strategy helps to address the issue of uveal biocompatibility in PIOL from a material design perspective, providing more economical and diversified options for patients with surgical needs. Statement of significance 1. A phakic intraocular lens material with high ocular biocompatibility has been prepared. 2. By introducing acrylic anhydride and activating it, collagen is covalently grafted onto the surface of HEMA without altering its structure. 3. By utilizing the collagen binding domain in the structure of fibronectin, selective adsorption of fibronectin is enhanced, forming extracellular matrix analogs that reduce macrophage M1 polarization and lower inflammation.
研究领域:
眼科
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生物材料学
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免疫学
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产品关联
DOI
PubMed
高影响因子
Heliyon
·
2024
·
IF 3.4
Yajun Wu,Hua Fan,Yuliang Feng,Jiasong Yang,Xiaobo Cen,Wensheng Li
Aim The aim of this study was to confirm the presence of the form deprivation myopia (FDM) guinea pig eye-gut axis and investigate the relationship between serum vasoactive intestinal peptide (VIP), lipopolysaccharides (LPS), specific gut microbiota and their metabolites. Method 20 specific-pathogen-free (SPF) guinea pigs were divided into the FDM and the control(Con) group. Following model induction, serum levels of VIP and LPS were quantified. A combination of 16S ribosomal ribosomal Ribonucleic Acid (rRNA) gene sequencing, non-targeted metabolomics and bioinformatics analysis were employed to identify disparities in gut microbiota and metabolites between the two groups of guinea pigs. Result Compared to the control group, FDM guinea pigs exhibited a significant trend towards myopia, along with significantly elevated concentrations of LPS and VIP ( p < 0.0001). Furthermore, Ruminococcus_albus emerged as the predominant bacterial community enriched in FDM ( p < 0.05), and demonstrated positive correlations with 10 metabolites, including l -Glutamic acid, Additionally, Ruminococcus_albus exhibited positive correlations with VIP and LPS levels ( p < 0.05). Conclusion The findings suggest that the Ruminococcus_Albus and glutamate metabolic pathways play a significant role in myopia development, leading to concurrent alterations in serum VIP and LPS levels in FDM guinea pigs. This underscores the potential of specific gut microbiota and their metabolites as pivotal biomarkers involved in the pathogenesis of myopia.
研究领域:
眼科学
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微生物学
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代谢组学
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PubMed
高影响因子
BMC Ophthalmology
·
2018
·Vol. 18
·期 1
·1-11
·
IF 1.77
Li Shanshan,He Jiangnan,Chen Qiuying,Zhu Jianfeng,Zou Haidong,Xu Xun
Background Our study aimed to investigate the ocular surface health of Shanghai University students. Methods This is a cross-sectional study carried out among freshmen and sophomores on the main campus of Shanghai University. Questionnaires including the widely-used ocular surface disease index (OSDI) and the Zung Self-rating Anxiety Scale (SAS) were completed first, and then ocular examinations were conducted regarding height & weight, blood pressure and heart rate, optometry, intraocular pressure exam, vision and subjective refraction, Aladdin, Macular pigment density measurement, tear test, anterior segment examination, fundus photography, ophthalmologist check, TOPCON OCT check, and Collin’s fundus blood test. Results Totally 901 students were involved in our five-day study. The prevalence of myopia was 92% (the spherical equivalent refraction (SER) < − 0.50 D), and that of high myopia was 23% (SER < − 6.0D). The prevalence of dry eye disease (DED) was 10%. The corneal epithelial loss rate (corneal fluorescein staining > 1) was 10%, and corneal sensation decline rate (≤ 30 mm) was 12%. 4.5% of subjects ( n = 40) had moderate or severe anxiety, 78% were mild and a small portion (17.5%) didn’t have anxiety at all. No statistical significant association was found between anxiety with DED, fluorescein staining or with corneal sensation (all p > 0.05). However, subjects with DED had more symptoms of anxiety. Results also showed that students who kept eye strain for a long time were more inclined to have DED (12.5%: 6.9%, p = 0.0407, 95% CI); those who watched mobile phones and/or computers for over eight hours daily were more vulnerable to DED and fluorescein staining than others (14.1%: 8.6%, p = 0.0129; 13.0%: 8.3%, p = 0.0233, 95% CI). Conclusions Keeping eye strain or near work for a long time is associated with DED, while students with DED tend to encounter anxiety symptoms. The prevalence of myopia in Chinese university students is still high. We consider it necessary to provide education to university students about the good eye-using habits, and to diagnose anxiety for student patients with DED.
研究领域:
眼科学
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公共卫生
·
流行病学
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视觉科学
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心理卫生
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屈光医学
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DOI
高影响因子
BMC Ophthalmology
·
2020
·Vol. 20
·期 1
·1-7
·
IF 1.413
Yu A-Yong,Shao Hui,Pan Anpeng,Wang Qinmei,Huang Zixu,Song Benhao,McAlinden Colm,Huang Jinhai,Chen Sisi
Background To investigate the biomechanical properties of the cornea in myopic eyes using corneal visualization Scheimpflug technology (Corvis ST). The relationships between the biomechanical properties of the cornea and the degree of myopia were also investigated. Methods 265 eyes of 265 subjects were included. Based on spherical equivalent (SE) in diopters (D), participants were divided into four groups: low myopia/control (SE: − 0.50 to − 3.00D), moderate myopia (SE: − 3.00 to − 6.00D), high myopia (SE: − 6.00 to − 10.00D) and severe myopia (SE greater than − 10.00D). Axial length (AL), anterior segment parameters, and corneal biomechanical properties were obtained with the Lenstar LS900, Pentacam HR and Corvis ST, respectively. Results Mean (±SD) SE was − 7.29 ± 4.31D (range: − 0.63 to − 25.75D). Mean AL was 26.31 ± 1.82 mm (range: 21.87 to 31.94 mm). Significant differences were detected within the four groups in terms of six corneal biomechanical parameters: deformation amplitude (DA), time from start until second applanation (A2-time), length of flattened cornea at the second applanation (A2-length), corneal velocity during the first and second applanation (A2-velocity), time from start to highest concavity (HC-time), and central curvature at highest concavity (HC radius). AL was positively associated with DA whereas negatively associated with A1-velocity and A2-length. SE was positively associated with A2-time, HC-time and A2-velocity, whereas negatively associated with DA. IOP was positively associated with four corneal biomechanical parameters and negatively associated with three parameters. Conclusions Eyes with severe myopia showed greater DA, lesser A2 time, HC time, and faster A2-velocity compared to low to high myopia. This suggests the cornea becomes weaker and more deformable with elongation of axial length with corresponding increases in myopia. DA, A2-time and A2-velocity could be useful corneal biomechanical indicators in patients with myopia.
研究领域:
眼科
·
视觉科学
·
生物力学
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屈光医学
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医学影像学
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DOI
高影响因子
Advanced Materials Technologies
·
2020
·Vol. 5
·期 12
·2000682
·
IF 5.969
Wenjing Zhang,Gongxian Dong,Haihua Feng,Suyan Shan,Libin Huang,Feng Yuan,Bijun Bao,Lu Yan,Zhaolin Xia,Tom Lawson,Jun Chen,Jia Qu,Yong Liu
Myopia is an irreversible visual disorder. Its pathogenic mechanism is not completely determined. This lack of understanding has led to difficulties in its early detection and prevention. Herein, it is reported that the content of dopamine (DA) inside tears is related to myopia diopter. Changes in DA are detected by the use of a wearable corneal biosensor. The biosensor is prepared by electrodeposition of the enzyme tyrosinase and poly (3,4-ethylenedioxythiophene) functionalized sulfur-doped graphene onto a self-designed corneal microelectrode. This leads to a high-performance biosensor with a high sensitivity (of 12.9 µA × 10 −3 m −1 cm −2 ), a good detection limit (of 101 × 10 −9 m ), excellent selectivity, and long-term stability. In vivo sensing tests on rabbits confirm a linear relationship between DA added to the animal eyes and the current response of the biosensor. Further tests on human tears sourced from defocus-induced myopia patients who have various myopia diopters show that the biosensor outputted a sensitive current signal that is related to the number of myopia diopters. This suggests there is an underlying relationship between myopia diopters and DA content in tears. It may provide a novel approach to a better understanding of myopia formation and its prevention.
研究领域:
眼科学
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生物传感技术
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材料科学
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医疗器械
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生物医学工程
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产品关联
DOI
高影响因子
JOURNAL OF CONTROLLED RELEASE
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2025
·
IF 11.5
Shan He,Pei-Juan Wu,Li Zhang,Jia-Xue Wu,Hou-Li Li,Kun Yi,Qiu-Yun Sun,Zi-Yang Wang,Yu-Xin Yue,Dong-Sheng Guo,Ke Hu,Xiao-Bei Huang,Wen-Juan Wan
Myopia is one of the most common visual impairments worldwide. Recent studies suggest that scleral hypoxia may act as an early trigger for myopia, initiating oxidative stress (OS) that disrupts extracellular matrix (ECM) remodeling and, in turn, drives axial elongation. In this study, we constructed a novel hypoxia-responsive host-guest complex, DOX@SAC4A, by encapsulating doxycycline (DOX) within sulfonated azocalix[4]arene (SAC4A). In vitro, this system significantly reduced DOX cytotoxicity and enabled efficient hypoxia-triggered drug release. Moreover, the intrinsic activity of SAC4A synergistically enhanced the antioxidant and anti-apoptotic effects of DOX, helping to maintain ECM homeostasis. In vivo studies further confirmed that DOX@SAC4A selectively targeted hypoxic sclera and markedly increased local drug accumulation. Consequently, it effectively suppressed myopia progression in guinea pigs by delaying axial elongation and alleviating myopic refractive shift. In conclusion, DOX@SAC4A is a multifunctional drug delivery system that offers both therapeutic effects and targeted delivery. These results present a promising candidate and an improved delivery strategy for myopia prevention and control.
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高影响因子
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