RSC Advances
·
2014
·Vol. 4
·期 10
·5130-5135
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IF 3.708
Kunjie Wang,Yanping Wu,Hongxia Li,Mingliang Li,Deyi Zhang,Huixia Feng,Haiyan Fan
We developed a one-step synthesis method of N-acetyl-L-cysteine and glutathione reduced protected Ag nanoparticles powder, and present a thorough characterization of the synthesized nanoparticles. The morphology and surface properties of Ag nanoparticles were obtained by transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FT-IR), the optical properties and stability of these Ag nanoparticles in air were studied by UV-vis spectroscopy. In addition, the Ag nanoparticles protected by N-acetyl-L-cysteine and glutathione reduced are used to investigate their antimicrobial efficacy and mechanism against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli, the antibacterial performance indicate that these Ag nanoparticles have superior stability and enhanced antibacterial activity compared to silver ions, it is believed that these Ag nanoparticles act as a promising antimicrobial material additive for practical applications.
研究领域:
纳米材料
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微生物学
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材料化学
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产品关联
DOI
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 12
·5006-5011
·
IF 1.821
Xifan Zheng,Chao Duan,Jiangtao Shen,Xuehui Duan
The spectrophotometric method is usually used in enzymatic synthesis of glutathione (GSH) for tracking detection of the reaction process. However, –SH from cysteine (Cys) interferes significantly with the analysis of GSH results. By multiple interference experiments, data processing and principle analysis, an anti-interference compensation mechanism has been found for the elimination of interference caused by the reaction of alloxan and Cys. An anti-interference compensation mechanism was set by making standard curves of absorbance, introducing into these the interference coefficient and compensation coefficient, and building an interference model and a compensation algorithm. The absorbance and derivative of the samples were detected at 320 nm, then the test data was imported into the anti-interference compensation flow chart: the interference ratio and compensation coefficient was obtained, and the content of GSH and Cys could be calculated, respectively. Detection results with the new method showed that when the concentration of Cys was no more than 4 times that of GSH in the reaction liquid, the recoveries of GSH and Cys were controlled to be 95–100% and 78–120% respectively. The disturbance of the detection results by Cys could be eliminated. The standard equation of GSH concentration–absorbance was y = 3.0629x + 0.0001, R2 = 0.9999. Compared with the usual alloxan method, the new method shows a significant increase in anti-interference characteristics and accuracy for detection of the GSH content and semi-quantitative analysis of Cys. It has practical application value for the quantitative detection of GSH, even without the separation of cysteine in the enzymatic synthesis of GSH.
研究领域:
生物化学
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分析化学
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分子生物学
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药物代谢
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酶工程
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Xieyin Li,Yuqian Zhang,Mian Wei,Manman Wang,Jing Wang,Guifu Zuo
A rod-like melem with high fluorescence quantum yield of 71.3 % was prepared in this work to enhance the chemiluminescence (CL) intensity of Na 2 SO 3 -Ce (Ⅳ) system. The results showed that the CL intensity of Na 2 SO 3 -Ce (Ⅳ) system could be increased by 350 times based on chemiluminescence resonance energy transfer (CRET) mechanism. Furthermore, a CL sensor based on Na 2 SO 3 -Ce (Ⅳ)-melem system was designed to detect reduced glutathione (G-SH). It was indicated that the CL sensor exhibited excellent G-SH detection performance with a detection limit of 0.065 nM and a linear range from 0.32 to 650 μM. This study applied melem for CL detection and provided a new way for the detection of G-SH.
研究领域:
分析化学
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生物医学
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DOI
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-7
·
IF 5.228
Zhang Zhen,Jiao Yuting,Wang Yuanyuan,Zhang Shusheng
A novel core-shell DNA self-assembly catalyzed by thiol-disulfide exchange reactions was proposed, which could realize GSH-initiated hybridization chain reaction (HCR) for signal amplification and molecules gathering. Significantly, these self-assembled products via electrostatic interaction could accumulate into prominent and clustered fluorescence-bright spots in single cancer cells for reduced glutathione monitoring, which will effectively drive cell monitoring into a new era.
研究领域:
生物化学
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分子生物学
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纳米技术
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癌症研究
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产品关联
DOI
PubMed
高影响因子
KOREAN JOURNAL OF CHEMICAL ENGINEERING
·
2018
·Vol. 35
·期 12
·2487-2495
·
IF 2.199
Jiao Zhiwei,Zhou Linjie,Wu Mengyuan,Gao Kang,Su Yanlei,Jiang Zhongyi
Inspired by the specific amino acid sequence Asn-Pro-Ala (NPA) of water channel aquaporins (AQPs), we fabricated polyamide (PA) nanofiltration (NF) membranes by introducing reduced glutathione (GSH) in interfacial polymerization (IP) method. Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectrometry (XPS), scanning electron microscope (SEM), atomic force microscopy (AFM), zeta potential and static water contact angle measurement were employed to characterize the chemical composition, morphology, electronegativity and hydrophilicity of the NF membranes. The water flux of GSH/PIP-TMC NF membrane reached 32.00 L m -2 h -1 at 0.2MPa, which was approximately twice than that of pristine PIP-TMC NF membrane when the ratio of GHS to piperazidine (PIP) was 40% during IP process. More water channels were built as GSH was embedded into PA layer. The fabricated NF membranes also took on potent rejection for dyes and Na 2 SO 4 . This study presents a simple and facile method to simulate water channels-based biological materials which may find potential application in water treatment.
研究领域:
膜科学与技术
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水处理工程
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材料化学
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纳米技术
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生物仿生材料
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分离科学
✓ 已验证
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Bin Wu,Xiangyun Wang,Fangyu Zhang,Yunjian Ma,Yonghua Wang
Photoinactivation has presented a substantial impediment to the practical implementation of fatty acid photodecarboxylase (FAP), despite its promising potential in the sustainable energy domain. In this study, a dual-promotion strategy for enhancing the photostability of FAP was used, including the introduction of internal reducing amino acids and the addition of external reducing agents. The best combination was to introduce the Cys mutation internally and add reduced glutathione (GSH) to the reaction system. The Cys mutants CvFAP-L173C, McFAP-S-A110C, and CsoFAP-S-L176C showed 2.65, 1.9, and 2.3-fold increased blue light half-life compared with their wild type, respectively. Notably, the Cys mutants performed significant photostability in a light-flow reactor containing 30 mM GSH, especially CsoFAP-S-L176C, which retained its functionality even after continuous reaction for 20 h, achieving a remarkable quantity of C15 alkane (200 mL, 6.9 mmol, conversion 93%). The positive impact of this strategy on FAPs underscores its considerable potential to enhance the photostability of FAPs and propel the application of FAPs in the field of biofuel synthesis.
研究领域:
生物化学
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生物技术
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可再生能源
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酶学
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分子生物学
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DOI
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
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2025
·
IF 3.1
Nho Soo Bin,Do Sang-Hun,Oh Seongjin,Park Yong-Cheol,Kim Sun-Ki
This study aimed to develop a non-genetically modified organism (non-GMO) Saccharomyces cerevisiae strain with enhanced glutathione production using UV-induced random mutagenesis. Among 30 wild-type strains, S. cerevisiae KACC 48331 was selected for its high glutathione production. A #14 mutant with significantly higher glutathione content was isolated via acrolein resistance screening. Optimal batch fermentation was established at 20 °C and pH 4.5 using a cost-effective medium of molasses and corn steep liquor (CSL). In fed-batch fermentation, the #14 mutant achieved 1.98 g/L of glutathione using molasses and CSL as carbon and nitrogen sources, comparable to yields from expensive complex media containing peptone and yeast extract. Notably, over 99% of the glutathione produced was in the reduced, biologically active form. These results demonstrate the feasibility of using non-GMO yeast and industrial by-products for sustainable and economical glutathione production.
研究领域:
生物技术
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微生物发酵
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工业微生物学
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代谢工程
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应用微生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Lulu Wang,Mingkui Shen,Qiaodan Hou,Zimei Wu,Jing Xu,Lin Wang
Diabetic individuals are frequently associated with increased fracture risk and poor bone healing capacity, and the treatment of diabetic bone defects remains a great challenge in orthopedics. In this study, an antioxidant hydrogel was developed using reduced glutathione grafted gelatine methacrylate (GelMA-g-GSH), followed by 3D printing to form a tissue engineering scaffold, which possessed appropriate mechanical property and good biocompatibility. In vitro studies displayed that benefitting from the sustained delivery of reduced glutathione, GelMA-g-GSH scaffold enabled to suppress the overproduction of reactive oxygen species (ROS) and reduce the oxidative stress of cells. Osteogenic experiments showed that GelMA-g-GSH scaffold exhibited excellent osteogenesis performance, with the elevated expression levels of osteogenesis-related genes and proteins. Further, RNA-sequencing revealed that activation of PI3K/Akt signaling pathway of MC3T3-E1 seeded on GelMA-g-GSH scaffold may be the underlying mechanism in promoting osteogenesis. In vivo, diabetic mice calvarial defects experiment demonstrated enhanced bone regeneration after the implantation of GelMA-g-GSH scaffold, as shown by micro-CT and histological analysis. In summary, 3D-printed GelMA-g-GSH scaffold can not only scavenge ROS, but also promote proliferation and differentiation of osteoblasts by activating PI3K/Akt signaling pathway, thereby accelerating bone repair under diabetes.
研究领域:
生物医学工程
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材料科学
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再生医学
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糖尿病研究
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骨科
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产品关联
DOI
PubMed
高影响因子
Journal of the Taiwan Institute of Chemical Engineers
·
2024
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IF 5.5
Xiao Duan,Qiang Wang,Wenfeng Che,Ting Li,Ke Zhang,Lingna Han,Lihua Song,Wei Guo
Background Doxorubicin (Dox) is a potent anticancer drug with severe side effects to normal tissues. To fabricate a nanomedicine with Dox is a better method to address the side effects of Dox in normal tissues. However, nanomedicines with unapproved materials could not be easily applied in clinic, hence, a nanomedicine fabricated by endogenous materials is a potential method to increase the possibility of clinical application. Methods In this study, a redox-responsive nanomedicine with clinical-applied anticancer of Dox and endogenous molecule of l -glutathione oxidized (GSSG) was prepared for cancer therapy. : The carboxyl and amino groups in l -glutathione oxidized can be reacted itself to form the poly- l -glutathione oxidized peptide-like polymer. Meanwhile, the Dox were conjugated in poly- l -glutathione oxidized polymer by amnio group in Dox and the structure of Dox-conjugated poly- l -glutathione oxidized polymer (Dox-Poly GSSG) was confirmed by FT-IR, NMR and MALDIT-TOF. Afterward, the drug loading of Dox (22 %) in poly-GSSG was measured by UV–vis and the accumulative Dox release rate of Dox-Poly GSSG is 46 % in 5 mg/mL reduced glutathione (GSH) environment at 72 h The size stability of Dox-Poly GSSG in PBS and PBS with GSH was evaluated by dynamic laser scatter (DLS) and transmit emission microscopy (TEM). Furthermore, cell viabilities of Dox-Poly GSSG were similar with free Dox evaluated by CCK-8 assay and flow cytometry experiment, respectively. The distribution of Dox-Poly GSSG in nuclei and tumor tissues was evaluated by Confocal laser scanning microscope and in vivo imaging system, respectively. Significant Findings The drug release results showed that the Dox could be controllable released in high concentration of GSH in tumor tissues. The tumor inhibition, H&E and TUNEL experiments showed that the Dox-Poly GSSG group can significantly inhibit the tumor growth compared to saline group and avoid major side effects to normal tissues compared to free Dox. This redox-responsive nanomedicine with clinical-applied anticancer of Dox and endogenous molecule of GSSG is a potential candidate for clinical application.
研究领域:
药学
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纳米技术
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癌症研究
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药物递送系统
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生物医学工程
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DOI
高影响因子
Stem Cell Research & Therapy
·
2019
·Vol. 10
·期 1
·1-11
·
IF 4.627
Liao Naishun,Shi Yingjun,Zhang Cuilin,Zheng Youshi,Wang Yingchao,Zhao Bixing,Zeng Yongyi,Liu Xiaolong,Liu Jingfeng
Background Adipose tissue-derived mesenchymal stem cells (ADSCs) are promising candidates for regenerative medicine. However, long-term in vitro passaging leads to stemness loss and cell senescence of ADSCs, resulting in failure of ADSC-based therapy. Methods In this study, ADSCs were treated with low dose of antioxidants (reduced glutathione and melatonin) with anti-aging and stem cell protection properties in the in vitro passaging, and the cell functions including stem cell senescence, cell migration, cell multidirectional differentiation potential, and ROS content were carefully analyzed. Results We found that GSH and melatonin could maintain ADSC cell functions through reducing cell senescence and promoting cell migration, as well as by preserving stemness and multidirectional differentiation potential, through inhibiting ROS generation during long-term expansion of ADSCs. Conclusions Our results suggested that antioxidant treatment could efficiently prevent the dysfunction and preserve cell functions of ADSCs after long-term passaging, providing a practical strategy to facilitate ADSC-based therapy.
研究领域:
再生医学
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干细胞生物学
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细胞衰老
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氧化应激
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2017
·
IF 3.887
Ruitao Cha,Juanjuan Li,Yang Liu,Yifan Zhang,Qian Xie,Mingming Zhang
Fe 3 O 4 nanoparticles with ultrasmall sizes show good T 1 or T 1 + T 2 contrast abilities, and have attracted considerable interest in the field of magnetic resonance imaging (MRI) contrast agents. For effective biomedical applications, the colloidal stability and biocompatibility of the Fe 3 O 4 nanoparticles need to be improved without reducing MRI relaxivity. In this paper, star polymers were used as coating materials to modify Fe 3 O 4 nanoparticles in view of their dense molecular architecture with moderate flexibility. The star polymer was composed of a β-cyclodextrin (β-CD) core and poly(2-(dimethylamino) ethyl methacrylate) (PDMAEMA) arms. Meanwhile, reduced glutathione (GSH), as a model drug, was also associated with the star polymer. Thus, a new platform for simultaneous diagnosis and treatment was achieved. Compared to the Fe 3 O 4 nanoparticles coated with linear polymers, the Fe 3 O 4 nanoparticles coated with star polymers (Fe 3 O 4 @GCP) possessed higher GSH association capacity and better stability in serum-containing solution. GSH could be released from Fe 3 O 4 @GCP nanoparticles in response to pH value of the solution. Since the sulfhydryl group on GSH is able to combine free radicals, Fe 3 O 4 @GCP nanoparticles exhibited less cytotoxicity compared to the Fe 3 O 4 nanoparticles without including GSH. Furthermore, the nanoparticles could also serve as good T 1 MRI contrast agent, and the MRI relaxivity of Fe 3 O 4 @GCP nanoparticles did not decrease after coated with the star polymer. These results indicate that the precisely designed Fe 3 O 4 @GCP nanoparticles could be used as a versatile promising theranostic nano-platform.
研究领域:
纳米技术
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材料科学
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生物医学工程
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磁共振成像
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药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Yidan Chen,Yao Zhang,Wenjun Dai,Yunxin Xue,Jiawei Li,Ke Zhang,Rongjun Tang,Chun Mao,Mimi Wan
Innovative treatments for peritoneal metastatic cancer have attracted widespread attention from researchers. Here, we propose a drug-loaded nanomotor (PSBMA/ l -Arg/DOX, PLD) based on zwitterionic materials for the treatment of peritoneal metastatic cancer through intraperitoneal injection. Zwitterionic polymer nanocarriers (PSBMA NPs) are obtained by radical polymerization with zwitterionic SBMA as the polymerization monomer and N,N’-Bis(acryloyl)cystamine (BAC) as the cross-linking agent. The zwitterionic substrate of this nanomotor has the ability to resist non-specific protein adsorption in ascites. The loaded l -arginine enables the nanomotor to have the ability to chemotaxis towards high concentrations of ROS/iNOS in tumors and be catalyzed to produce NO, achieving deep penetration into tumor tissue. Furthermore, the disulfide bond (S S) carried by the crosslinking agent used in the preparation of the nanomotor can respond to the high expression of reducing glutathione in the tumor microenvironment and undergo degradation, releasing a large amount of loaded drug DOX. Cell and animal disease model experiments confirme the good therapeutic effect of this drug-loaded nanomotor, providing new therapeutic concepts and strategies for the treatment of peritoneal metastatic cancer.
研究领域:
癌症研究
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纳米技术
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药学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）