Di Weng,Sheng-Hua Zha,Yuan Zhu,Hang Li,Shou-Bu Hou,Qing-Sheng Zhao,Bing Zhao
Four powders of Forsythia suspensa leaf were prepared by coarse grinding and subsequent ball milling . The effects of particle size on the physicochemical and antioxidant properties of the powders were evaluated. The results showed that the particle size of the powder decreased from 107.99 to 29.20 μm by ball milling for 5 h. In addition, the ball-milled powders had substantial advantages in water holding, solubility and swelling capacities. Thus, the ball-milled powders had a better dissolution of phenolic, flavonoid , and polysaccharide components (25.45, 1.99 and 44.19 mg/g of powder for 5 h of ball milling), ultimately resulting in a superior antioxidant activity (maximum reductions of 14.96% and 19.20% for DPPH and ABTS radicals, respectively). The FTIR analysis showed that the structure of the active ingredients remained intact after ball milling. In conclusion, ball milling is a promising technique for preparing Forsythia suspensa leaf powder with improved physicochemical and biological properties.
Deep eutectic solvents (DESs) are showing broad application prospects in separation of bioactive components from natural products because of their eco-friendly advantages. In this study, a green method for effective extraction and reThe ultrasound-assisted extraction is optimized using Box-Behnken designcovery of three bioactive lignans from the leaves of Forsythia suspensa was developed for the first time using ultrasound-assisted deep eutectic solvents extraction (UAE-DES) and macroporous resin enrichment. Choline chloride-ethylene glycol (molar ratio of 1:3) with 20 % (v/v)water was confirmed as the optimal solvent. The optimized conditions were obtained by response surface methodology: liquid to solid ratio of 30 mL/g, extraction temperature of 30℃, ultrasound power of 200 W and extraction time of 30 min. The extraction yields of phillyrin, pinoresinol and phylligenin were 6.70, 9.65 and 45.77 mg/g, respectively, which were significantly higher than those of conventional organic solvents. Furthermore, the target compounds were recovered and purified from DES extracts using macroporous resin of HPD100. The recovery efficiency of phillyrin, pinoresinol and phylligenin was 87.9 %, 95.0 % and 91.7 %, respectively. After purification by the resin the content of phillyrin, pinoresinol and phylligenin in the recovered dry extract was 10.6 %, 12.8 %, and 64.8 %, respectively. This study provided an efficient and green approach for extraction and preparative separation of active lignans from natural products.
危险品化学品经营许可证（带存储）