JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2025
·
IF 3.1
Zhiyong Wu,Xinhao Wang,Lin Wang,Na Sun,Zihui Yang,Jianguo Zeng
Allocryptopine (ALL), a principal active component of the novel veterinary medicine Bopu Powder®, has gained widespread application in the poultry farming sector for the effective management of Escherichia coli ( E. coli ) diarrhea. In order to explore the metabolites and the pivotal enzymes associated with ALL, this study was conducted employing an in vitro chicken liver microsomal incubation. The metabolites of ALL were analyzed and identified by combining isotope tracing technology with the application of mass spectrometry fragmentation patterns. The key metabolic enzymes involved in the biotransformation of ALL were explored using the CYP450 recombinant enzyme method, which facilitated the identification of the enzymes contributing to ALL's metabolic pathway. The liver microsomal metabolism investigation revealed a total of five metabolites, with the predominant being M2 (harmol or 3-hydroxy-4-methoxy-6-methyl-5,7,8,15-tetrahydro-[1,3]dioxolo[4',5':4,5]benzo[1,2-g]benzo[c]azecin-14(6H)-one). The recombinant enzyme analysis conclusively identified CYP2D6 as the pivotal CYP450 isoenzyme that plays a central role in the metabolic pathway of the principal ALL metabolite, M2. This research not only expands our comprehension of the biotransformation process of ALL but also provides significant scientific evidence for the clinical safety of ALL, which was of great importance for guiding the application of ALL in the field of veterinary medicine.
研究领域:
兽医学
·
药代动力学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Inventions
·
2019
·Vol. 4
·期 4
·65
·
IF 0
Fiorency Santoso,Bonifasius Putera Sampurna,Yu-Heng Lai,Sung-Tzu Liang,Erwei Hao,Jung-Ren Chen,Chung-Der Hsiao
This study aimed to develop a simple and cost-effective method to measure blood flow in zebrafish by using an image-based approach. Three days post fertilization (dpf) zebrafish embryos were mounted with methylcellulose and subjected to video recording for tracking blood flow under an inverted microscope equipped with a high-speed CCD camera. In addition, Hoffman lens was used to enhance the blood cell contrast. The red blood cell movement was tracked by using the TrackMate plug-in in the ImageJ image processing program. Moreover, Stack Difference and Time Series Analyzer plug-in were used to detect dynamic pixel changes over time to calculate the blood flow rate. In addition to blood flow velocity and heart rate, the effect of drug treatments on other cardiovascular function parameters, such as stroke volume and cardiac output remains to be explored. Therefore, by using this method, the potential side effects on the cardiovascular performance of ethyl 3-aminobenzoate methanesulfonate (MS222) and 3-isobutyl-1-methylxanthine (IBMX) were evaluated. MS222 is a common anesthetic, while IBMX is a naturally occurring methylxanthine. Compared to normal embryos, MS222- and IBMX-treated embryos had a reduced blood flow velocity by approximately 72% and 58%, respectively. This study showed that MS222 significantly decreased the heart rate, whereas IBMX increased the heart rate. Moreover, it also demonstrated that MS222 treatment reduced 50% of the stroke volume and cardiac output. While IBMX decreased the stroke volume only. The results are in line with previous studies that used expensive instruments and complicated software analysis to assess cardiovascular function. In conclusion, a simple and low-cost method can be used to study blood flow in zebrafish embryos for compound screening. Furthermore, it could provide a precise measurement of clinically relevant cardiac functions, specifically heart rate, stroke volume, and cardiac output.Keywords:blood flow tracking;ImageJ;zebrafish;IBMX;MS222;cardiovascular function assessment
研究领域:
发育生物学
·
毒理学
✓ 已验证
产品关联
DOI
Crystals
·
2022
·Vol. 12
·期 8
·1027
·
IF 2.67
Ningzhe Yan,Hao Luo,Yanan Liu,Haiping Yu,Guangyin Jing
Regulating the swimming motility of bacteria near surfaces is essential to suppress or avoid bacterial contamination and infection in catheters and medical devices with wall surfaces. However, the motility of bacteria near walls strongly depends on the combination of the local physicochemical properties of the surfaces. To unravel how nanostructures and their local chemical microenvironment dynamically affect the bacterial motility near surfaces, here, we directly visualize the bacterial swimming and systematically analyze the motility ofEscherichia coliswimming on ZnO nanoparticle films and nanowire arrays with further ultraviolet irradiation. The results show that the ZnO nanowire arrays reduce the swimming motility, thus significantly enhancing the trapping ability for motile bacteria. Additionally, thanks to the wide bandgap nature of a ZnO semiconductor, the ultraviolet irradiation rapidly reduces the bacteria locomotion due to the hydroxyl and singlet oxygen produced by the photodynamic effects of ZnO nanowire arrays in an aqueous solution. The findings quantitatively reveal how the combination of geometrical nanostructured surfaces and local tuning of the steric microenvironment are able to regulate the motility of swimming bacteria and suggest the efficient inhibition of bacterial translocation and infection by nanostructured coatings.Keywords:ZnO nanowire arrays;bacteria motility;antibacterial;particle tracking
研究领域:
微生物学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Energy & Environmental Science
·
2024
·Vol. 17
·期 11
·3807-3818
·
IF 32.4
Shenyang Xu,Zhefeng Chen,Wenguang Zhao,Wenju Ren,Chenxin Hou,Jiajie Liu,Wu Wang,Chong Yin,Xinghua Tan,Xiaobing Lou,Xiangming Yao,Zhihai Gao,Hao Liu,Lu Wang,Zuwei Yin,Bao Qiu,Bingwen Hu,Tianyi Li,Cheng Dong,Feng Pan,Mingjian Zhang
As commercial cathodes with the highest practical capacity, the structural nature of Li-rich Mn-based (LMR) cathodes, composite or solid solution, is still under debate. Due to the extreme structural similarity of two layered-phase components, namely, the monoclinic phase (C2/m) and rhombohedral phase (Rm), no single tool can resolve this concern alone. Herein, we combined multiple advanced techniques to comprehensively study the structural changes during synthesis of LMR cathodes from different aspects such as the elemental distribution, local structure, long-range structure, and short-range structure, revealing a clear process from the formation of two phases to the gradual phase fusion and eventually to a nearly solid solution. Particularly, X-ray pair distribution function (PDF) analysis combined with theoretical simulations reveals for the first time that the transition metal (TM)–TM distance increases with the progress of the phase fusion, which makes the short-range structural change in TM–TM atomic pairs an effective parameter for judging the extent of phase fusion. Eventually, excellent electrochemical performance was achieved by balancing capacity and cycling stability through adjusting the phase fusion to a medium extent in lithium and sodium two layered-phase components. This study establishes an approach to investigate the structural evolution in the complicated multiple-phase system and provides valuable insights into the design and optimization of cathodes by tuning the phase fusion extent.
研究领域:
材料科学
·
电化学
·
能源存储
✓ 已验证
DOI
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2020
·
IF 5.647
Yong Liu,Jingfu Wang,Jingan Chen,Zuxue Jin,Shiming Ding,Xiaohong Yang
The phosphate oxygen isotope (δ 18 O P ) ratio has been proven to be an effective tool to trace the sources and biogeochemical cycles of phosphorus (P) in aquatic ecosystems . However, the enrichment of phosphate (PO 4 ) and the removal of impurities are quite complex and easy to cause PO 4 loss in current δ 18 O P analytical methods. Moreover, the δ 18 O P value obtained by the commonly-used instantaneous sampling method is more of the instantaneous information of P, which is accidental or uncertain for accurate identification of the P source. In this study, a new method of in situ enrichment, elution, and purification of PO 4 (ISEEP) was developed for δ 18 O P analysis in waters. This method utilized a PO 4 binding phase (Zr-Oxide gel) to selectively in situ adsorb PO 4 in water and exhibited an adsorption capacity per unit area of up to 789.3 μg P/cm 2 . The PO 4 on the gel was eluted easily with a 1 M NaOH solution. More than 99.7% of the common anions, cations, and dissolved organic matter (DOM), as well as more than 90% of the trace elements were removed synchronously after adsorption and elution of PO 4 . The recovery rate of PO 4 in the whole procedure was as high as 92.8%. The XRD and SEM examinations showed that the ISEEP can obtain high-purity Ag 3 PO 4 solid for the δ 18 O P measurement. The reliability of the ISEEP method is confirmed by the measured δ 18 O P value and standard deviation of parallel samples from different types of natural waters obtained by both the ISEEP and the current popular McLaughlin (2004) method. It provides a good prospect of this new method for tracing the P sources and their biogeochemical cycling in aquatic ecosystems.
研究领域:
环境科学
·
生物地球化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2017
·Vol. 5
·期 9
·1820-1827
·
IF 4.21
Dan Shao,Mengmeng Lu,Duo Xu,Xiao Zheng,Yue Pan,Yubin Song,Jinying Xu,Mingqiang Li,Ming Zhang,Jing Li,Guangfan Chi,Li Chen,Bai Yang
Mesenchymal stem cells (MSCs) hold great potential for tissue engineering and regeneration medicine. However, for clinical use, MSCs may be detrimental due to their uncertain fate during the transplantation. It is therefore highly desirable to develop biocompatible nanomaterials to integrate cell fate regulation with monitoring for MSC-based therapy. Herein, we employ recently developed citric acid-based carbon dots (CDs) and their derivatives (Et-IPCA) for labeling and tracking of rat bone marrow mesenchymal stem cells (rBMSCs). We further investigate their biocompatibility and effects on the osteogenic differentiation of rBMSCs. These highly fluorescent probes provide labeling of rBMSCs by internalization without affecting cell viability or inducing apoptosis when the concentration is lower than 50 μg mL−1. Importantly, the presence of the CDs and Et-IPCA facilitates high-efficiency osteogenic differentiation of rBMSCs by promoting osteogenic transcription and enhancing matrix mineralization. Compared to Et-IPCA, CDs considerably provide long-term tracking and promote the differentiation of rBMSCs toward osteoblasts through the ROS-mediated MAPK pathway. Taken together, our results consistently demonstrate that carbon dots are capable of both tracking and enhancing the osteogenic differentiation of MSCs. This study sheds new light on the potential of carbon dots as a bifunctional tool in the thriving field of MSC-based therapy.
研究领域:
组织工程
·
再生医学
·
纳米技术
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Juan Xu,Hongpo Zhou,Kun Li,Jinju Ma,Wenwen Zhang,Lanxiang Liu,Yanlin Sun,Hong Zhang,Kai Li
The smart polyphenol material could be designed to form two competitive interactions related to same one polyphenol molecule with different objects that could easily adhere to one surface through the weak interaction, and also could be entirely removed from the same surface by the strong interaction. In this study, the multi-scale pectin beads were fabricated by crosslinking with ions that could be acted as polyphenol loading agent through electrostatic force between the ions loading on the surface of pectin beads and polyphenol. The two effects on the size and appearance of pectin beads were detected. Because of the hydrogen bond between polyphenols loading on the beads and the surface of target, fluorescence-functionalized beads could easily adhere on target surfaces. Meanwhile, attributing to stronger electrostatic force between surface ions on beads and polyphenol, the thin membrane made of the beads can be entirely removed from the target surface to avoid the pollution of fluorescence probes.
研究领域:
材料科学
·
生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Jiaqi Zhang,Haohao Li,Yanrong Li,Shanshan Li,Yang Xu,Huanrong Li
Microplastics (MPs) pollution is considered as one of the most serious threatens to environment and human health. How to identify and trace MPs rapidly is still a challenge. Here, we proposed a simple method to prepare boron-doped carbon nanoparticles (BCNPs) through solvothermal reaction between nitrated pyrene and boric acid for identifying and tracing MPs. These BCNPs exhibited a hydrophobic affinity to plastics and endow plastics with fluorescence emission. Interestingly, we can distinguish polypropylene (PP, strong yellow emission); polyethylene (PE, weak yellow emission); polyvinyl chloride (PVC, strong magenta emission); and polystyrene (PS, weak pink emission) according to fluorescence emission color and intensity upon 365 nm excitation, using these BCNPs via simple adsorption process. Discrepant color emissions were observed in different types of MPs due to plastics medium influence on the surrounding environment of BCNPs. As a result of no fluorescence from single BCNPs, BCNPs can be used as MPs tracing probe in a “turn-on” fluorescence mode caused by combination of BCNPs and MPs. The practical ability of BCNPs for MPs was validated in soil and aqueous models, and semi-quantification was performed using ImageJ software. The described tracing method can be carried out using UV light without any complex instruments, provide an important step toward tracing MPs rapidly in natural conditions.
研究领域:
环境科学
·
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
COMPOSITES PART B-ENGINEERING
·
2021
·
IF 9.078
Xiyu Li,Qin Zou,Jiawei Wei,Wei Li
Scaffold degradation regulation has become the key aspects in promoting bone regeneration and reconstruction. In the study, a novel stepwise-degraded PLGA/PCL/HA:Yb/Ho/Zn (PPHZ) scaffold was designed to compare with the fast-degradable PLGA/HA:Yb/Ho/Zn (PLHZ) scaffold to investigate the effects of scaffold degradation regulation on osteogenesis and to trace the scaffold degradation process. Micro-CT reconstruction, confocal fluorescence imaging, and histological sections were used for the analyses of the harvested animal samples, which demonstrated that the degradation regulated stepwise-degraded PPHZ scaffold maintaining higher mechanical strength and longer scaffold integrity is more advantageous for in vivo osteogenesis than the fast-degradable PLHZ scaffold. Meanwhile, the multifunctional HZ particles would help the anti-infection and tracking the distribution during scaffold degradation. The PPHZ scaffold may benefit future repair of anti-infective bone defects and in vivo multimodal tracking.
研究领域:
生物医学工程
·
组织工程
·
生物材料科学
·
骨科研究
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry C
·
2021
·Vol. 9
·期 8
·2864-2872
·
IF 7.393
Hao-Yuan Wang,Kang-Kang Yu,Chong-Yun Tan,Kun Li,Yan-Hong Liu,Lei Shi,Kai Lu,Xiao-Qi Yu
It is still a challenge to develop new intelligent multifunctional fluorescence reagents with high brightness, high optical stability, and high contrast fluorescence based on small molecules. In this study, by building push–pull electronic structures and adjusting the number of carbazole groups and the length of the alkyl chain, a series of multifunctional molecules based on a purine-core has been successfully synthesized and achieved the improvement of the fluorescence quantum yield. Furthermore, the relationship between the structure and optical properties of these purine derivatives has been investigated from multiple perspectives. Moreover, the most promising molecule (CPPC) exhibits high fluorescence quantum yield, high brightness, high optical stability, and high contrast fluorescence, and it has been successfully applied in information encryption, anti-counterfeiting and LD-tracking of living cells. The present study offers a novel and efficient approach to develop multifunctional fluorescence materials with high performance based on purine.
研究领域:
化学
·
材料科学
·
生物学
✓ 已验证
DOI
高影响因子
ACS Sensors
·
2025
·
IF 9.1
Kai Dong,Yihan Zhang,Xiaoxuan Fan,Leo N. Y. Cao,Xiao Peng
Underwater ultrasonic detection is critical for marine security, playing a vital role in resource development, environmental protection, and national defense. However, existing detection systems, which primarily rely on active scanning technologies, are hindered by high costs, significant energy demands, and challenges in achieving large-scale deployment. Here, we introduce a microfiber-based triboelectric acoustic sensor (MTAS) featuring a core–shell hierarchical structure, offering a self-powered solution for precise measurement of underwater ultrasound source distance. By leveraging the principles of contact electrification/triboelectrification and electrostatic induction, the MTAS efficiently converts complex ultrasonic vibrations into real-time electrical signals. The MTAS demonstrates rapid response times as low as 8.6 μs, a high signal-to-noise ratio of 29.8 dB, and the capability to detect ultrasonic sources with power levels above 1.6 W via time-difference-of-arrival analysis. To address large-scale sea applications, we further propose a distributed network that integrates multiple MTAS units capable of precise ultrasonic source localization and real-time motion trajectory visualization. This innovation represents a transformative approach, combining self-powered operation, ease of deployment, and high imperceptibility, paving the way for large-area, energy-efficient submarine security systems. Such advancements redefine the paradigm of underwater target detection, aligning technological innovation with the pressing demands of marine safety and environmental sustainability.
研究领域:
海洋技术
·
传感器技术
·
能源技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2020
·Vol. 25
·期 23
·5708
·
IF 3.267
Jing Zhao,Hongjie Luo,Xiao Huang
In order to visually display the migration and crystallization process of salt solution in porous cultural relics, copper sulfate solution with color rendering property was selected to record the migration, crystallization and resolution of salt solution in simulated SiO2samples under different environmental conditions in real time through high-resolution recording system, scanning electron microscope system, salt phase X-ray diffraction system, and so on. The results showed the migration of salt solution in porous samples was related to the structural characteristics of the porous samples, the migration rate of salt solution, the evaporation rate and the change frequency of crystallization–resolution, etc., in which the large pore size of the sample, the higher the concentration and the faster migration and evaporation rate of salt solution, the greater the change rate of the brine accumulation zone or salt crystallization zone in the different porous samples. During the humidification–drying cycles of rainfall, the higher the cycle frequency of humidification–drying was, the higher the drying temperature was, the more frequent the crystallization-analysis change of salt in the salt-bearing sample was, and the more extensive the distribution of salt crystal zone was. This is the first time to visualize the salt belt by simulating the changing process of a salt solution with a color rendering property in porous samples. This has scientific theoretical guidance for the study of the migration–crystallization changes of soluble salts contained in porous silicate cultural relics. The visibility analysis results of multilayer salt crystal belts can also provide the preliminary basis for further effective desalination of salt bearing cultural relics.Keywords:color rendering tracing;copper sulfate;porous cultural relics;crystalline brine accumulation zone;salt crystalline zone
研究领域:
文物保护
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）