iScience
·
2023
·
IF 5.8
Haocheng Tang,Yichi Zhong,Jingzhou Li,Luyang Hou,Xizhe Liang,Jiahao Zhang,Hongyu Yang,Hongxing Dong,Long Zhang
Benefiting from the high modulation bandwidth (BW), low energy consumption and excellent optical performance, lead halide perovskite has attracted wide attention in visible light communication (VLC). However, the ion migration which results in mobile point defects in perovskite structures is recognized as a crucial key factor inducing the performance degradation. Here, the influence of ion migration in perovskite devices on the performance of VLC was systematically studied. The ion migration process is realized by mixing CsPbBr 3 and CsPbI 3 quantum dots, during which, the performance of the VLC system is reduced, but it can return to its initial state after stabilization. The on-off keying (OOK) modulation scheme of the perovskite light-emitting diode (LED) device was carried out, achieving a data rate of 90 Mbps.
研究领域:
材料科学
·
通信工程
·
光学工程
✓ 已验证
DOI
高影响因子
Frontiers in Microbiology
·
2019
·
IF 4.259
Chen Xiao,Zhang Zheng,Liu Xiaoxia,Cui Bo,Miao Wentao,Cheng Weiwei,Zhao Fengyun
Volvariella volvacea is a typical edible Basidiomycete with a high-temperature tolerance. It has a strong fibrinolysis capability and consumes abundant agricultural wastes. In agricultural cultivation, mycelial subculturing has been adopted, leading to serious strain degeneration. In this study, continuous mycelial subculturing of the common V. volvacea strain V971 (original strain recorded as M0) was performed in potato dextrose agar (PDA) medium. One generation of the strain was preserved every 3 months (90 days); thus, six generations of degenerated strains (M1–M6) were obtained after 18 months of mycelial subculturing. The original and degenerated strains were preserved in sterile paraffin liquid at room temperature (18–25°C). The biological traits and nutrients of M0 and M1–M6 were studied. The mycelial growth rate and biomass initially increased and then decreased as the degeneration progressed, reaching minimum levels of 0.041 ± 0.001 cm/h and 1.82 ± 0.25 g, respectively, at M6. Additionally, the polysaccharide, protein, polyphenol, flavone, total amino acid, and total mineral element contents of the strains decreased continuously, reaching minimum levels of 30.12 ± 3.12 g/100 g, 26.42 ± 2.1 g/100 g, 1.08 ± 0.05 g/100 g, 4.23 ± 0.21 g/100 g, 12.51 mg/g, and 398.05 mg/kg, respectively, at M6. The decolorization capability of V. volvacea in liquid medium supplemented with bromothymol blue and lactose reflected the degree of strain degeneration, with the capability weakening as the degeneration intensified. These results are highly significant for V. volvacea production. The mycelial characteristics during subculture-associated degeneration were described and provide an early identification method for V. volvacea’s degeneration.
研究领域:
真菌学
·
食用菌研究
·
农业微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYTOMEDICINE
·
2023
·
IF 7.9
Yulin Qi,Lu Liu,Dan Liang,Shiyun Tang,Xiaoyi Yu,Hejiang Ye,Nianzhi Chen
Background Retinitis pigmentosa (RP) refers to a group of progressive photoreceptor degenerative diseases . The activation of microglia has been reported to play an important role in the photoreceptor degeneration in RP retinal. Bujing Yishi tablets (BJYS), a Chinese herbal medicine , has been used to treat retinal diseases in China with desirable effect in improving visual function. However, the mechanisms underlying the efficacy of BJYS treatment in RP are not yet fully understood. Purpose Based on the preliminary experiments, this study aimed to investigate the therapeutic mechanism involved in treating N -Methyl- N -Nitrosourea (MNU)-induced retinal degeneration of RP with BJYS. Methods To explore the efficacy of BJYS, a rat experimental RP model was established through intraperitoneal injection of MNU (50 mg/kg). Two experiment was carried out. After the treatment, we conducted H&E, TUNEL , retinal cytokine levels and IBA-1 expression in microglia to confirm the impact on RP model. The specific mechanism of action of BJYS tablet was assessed by western blot , real-time polymerase chain reaction (RT-PCR), and immunofluorescence to determine the mRNA and protein expression levels involved in clarifying the effectiveness of BJYS exerted through P2X7R/CX3CL1/CX3CR1 pathway. Results Significant alleviation of retinal morphological structure and photoreceptor degeneration by BJYS treatment was observed in the retinal of MNU-induced RP rats, BJYS prevented the reduction of ONL thickness and decreased the level of apoptotic cells in ONL. It also inhibited microglia overactivation and reduced retinal levels of IL-1β, IL-6, TNF-α. In addition, BJYS decreased the protein expression and mRNA expression of P2X7, CX3CL1 and CX3CR1 and reduced the phosphorylation of p38 MAPK . Conclusion In summary, this study suggested that BJYS treatment could alleviate photoreceptors degeneration of RP by inhibiting microglia overactivation and inflammation through the P2X7R/CX3CL1/CX3CR1 pathway. These effects suggest that BJYS tablets may serve as a promising oral therapeutic agent for RP.
研究领域:
眼科学
·
药理学
·
神经科学
·
中医
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Fungi
·
2025
·Vol. 11
·期 7
·513
·
IF 4
Tengyu Ma,Hongguang Zhu,Jiajia Yin,Yu Tian,Wenjing Yan,Haixin Sun
Saccharomyces cerevisiaeoften undergoes strain degeneration during industrial serial subculturing, though this phenomenon remains understudied. This study first conducted strain screening and biological characterization through TTC (2,3,5-triphenyltetrazolium chloride) colorimetric assays, Durham tube fermentation gas production tests, and WL medium (Wallerstein Laboratory medium) cultivation. Subsequently, the changes in intergenerational biological traits after serial subculturing were investigated. Finally, transcriptomic analysis was employed to examine differential gene expression under high-glucose stress during continuous subculturing. The experimental results demonstrated that: (1) TheS. cerevisiaeQDSK310-Z-07 (GenBank: PP663884), isolated from farm soil, exhibited robust growth within a temperature range of 24–36 °C, with optimal growth observed at 28 °C. It thrived in a pH range of 4–5.5 and efficiently utilized various carbon and nitrogen sources; (2) After serial subculturing, the strain’s ethanol production capacity and fermentation rate partially declined and then stabilized, while maintaining strong tolerance to high ethanol concentrations and hyperosmotic stress; (3) Transcriptomic analysis revealed significant differential expression of genes related to lipid metabolism, amino acid metabolism, and other pathways under high-glucose stress following continuous subculturing. These findings elucidate the biological trait variations inS. cerevisiaeduring serial subculturing and provide key metabolic regulation candidate targets for its long-term adaptive evolution under high-glucose stress.
研究领域:
微生物学
·
分子生物学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 6
·899
·
IF 5.719
Xiaoyan Wu,Wei Li,Qingrong Chen,Caixia Xu,Jiamian Wang,Lingyuan Wu,Guodong Liu,Weiping Wang,Ting Li,Ping Chen,Long Xu
Light-induced degradation (LID) phenomenon is commonly found in optoelectronics devices. Self-healing effect in halide lead perovskite solar cells was investigated since the electrons and holes in the shallow traps could escape easily at room temperature. However, the degradation in the semiconductors could not easily recover at room temperature, and many of them needed annealing at temperatures in the several hundreds, which was not friendly to the integrated optoelectronic semiconductor devices. To solve this problem, in this work, LID effect of photocurrent in p-type Mg-doped gallium nitride thin films was investigated, and deep defect and vacancy traps played a vital role in the LID and healing process. This work provides a contactless way to heal the photocurrent behavior to its initial level, which is desirable in integrated devices.Keywords:Mg-doped gallium nitride;light-induced degradation;photocurrent device;contactless healing
研究领域:
材料科学
·
光电子学
·
半导体物理
✓ 已验证
DOI
PubMed
高影响因子
Molecular Therapy-Nucleic Acids
·
2023
·
IF 8.8
Chen Jun Guo,Xiu Li Cao,Yu Fei Zhang,Kang Yi Yue,Jing Han,Hong Yan,Hua Han,Min Hua Zheng
Inherited and age-related retinal degenerations are the commonest causes of blindness without effective treatments. Retinal progenitor cells (RPCs), which have the multipotency to differentiate into various retinal cell types, are regarded as a promising source of cell transplantation therapy for retinal degenerative diseases. However, the self-limited expansion of RPCs causes difficulty in cell source supply and restrict its clinical treatment. In this work, we found that inhibition of microRNA-449a (miR-449a) in RPCs can promote proliferation and inhibit apoptosis of RPCs, partially through upregulating Notch signaling. Further optimization of transduction miR-449a inhibitor into RPCs by endothelial cell-derived exosomes can promote the survival of RPCs transplanted in vivo and reduce cell apoptosis in retinal degeneration mouse models. In summary, these studies have shown that exosome-miR-449a inhibitor can effectively promote the expansion of RPCs in vitro and enhance transplanted RPCs survival in vivo , which might provide a novel intervention strategy for retinal degenerations in the future.
研究领域:
医学
·
分子生物学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2025
·Vol. 26
·期 8
·3735
·
IF 4.9
Xiyuan Liu,Zhaoze Ni,Jing Zhang,Xiaoyan Lin,Chenxin Wu,Yuyang Wu,Lingqin Dong,Zongduan Zhang,Zai-Long Chi
The retinal pigment epithelium (RPE) serves as a critical guardian of subretinal homeostasis, with its dysfunction implicated in major retinal pathologies, including age-related macular degeneration (AMD) and retinitis pigmentosa. While cellular senescence has emerged as a key driver of RPE degeneration, the molecular mechanisms underlying this process remain incompletely defined. Emerging evidence implicates dual-specificity phosphatase 4 (DUSP4) in cellular stress responses through its antioxidant and anti-inflammatory capacities, yet its role in RPE pathophysiology remains unexplored. Our study reveals a compensatory increase in DUSP4 expression during AMD-associated RPE senescence. To functionally characterize this observation, we knocked down DUSP4 in the RPE of mice via subretinal injection of AAV-shDUSP4. In a sodium iodate-induced dry AMD model, mice with DUSP4 knockdown presented more severe visual impairment than control mice did. To further investigate the molecular mechanism, stable DUSP4-knockout cell lines were constructed via CRISPR/Cas9 technology. The high expression of senescence markers in the DUSP4-knockout cell lines was reversed by DUSP4 overexpression. Furthermore, DUSP4 coordinates the modulation of cell cycle, stress response, and pro-inflammatory signaling by inhibiting the p53, p38, and NF-kB pathways. These findings establish DUSP4 as a multi-functional regulator of RPE senescence. Our work not only elucidates a novel DUSP4-dependent mechanism in AMD pathogenesis but also highlights its therapeutic potential for preserving RPE function in AMD.
研究领域:
眼科学
·
分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Fundamental Research
·
2024
·
IF 5.7
Xuewen Wang,Song Hong,Guirong Tang,Chengshu Wang
Filamentous fungi frequently degenerate during subculturing, which manifests as the reduction or loss of conidiation, sexuality, secondary metabolite production, and/or virulence against hosts. The underlying mechanism of spontaneous fungal degeneration is still elusive. In this study, the fluffy mycelium-type sector variants formed by three ascomycete fungi were transferred and found to show the typical features of culture degeneration. The variant cells were evidenced with the accumulation of reactive oxygen species (ROS), and the ROS-associated formation of hyphal coils. Genome resequencing of these sector cultures identified substantial random mutation sites in each variant in a trend associated with fungal reproduction style. The high bias towards transversions over transitions was similarly detected in degenerate genomes. Otherwise, a higher number of mutations were accumulated in the intergenic regions of the Metarhizium robertsii and Cordyceps militaris sector genomes, whereas the exonic regions of the Aspergillus nidulans variant genes were detected with a higher mutation rate. Unexpectedly, none of those mutated genes had orthologous relationships among the three sectors, while only a few of them were shared between two fungi. A few transcription factor genes with frameshift mutations in sectors were selected for deletions in parental strains, and the null mutants demonstrated the varied degrees of degenerate phenotypes. In addition to reasoning the causal mechanism of fungal degeneration, our data provide insights to better maintain and monitor fungal culture stability.
研究领域:
微生物学
·
遗传学
·
分子生物学
✓ 已验证
DOI
高影响因子
Advanced Optical Materials
·
2022
·Vol. 10
·期 16
·2200903
·
IF 10.05
Siyuan Huang,Renfu Zhang,Peipei Shao,Yiwen Zhang,Rui-Tao Wen
Electrochromic performance fading is inevitable upon extended cycling, and the limited lifetime greatly prevents large-scale commercialization of electrochromism-based devices. Here the origin of the performance degradation is uncovered and the performance restoration in TiO 2 thin films is explored. It is shown that, depending on the applied potential windows, the inserted ions are immobilized in α - traps resulting in a monotonous degradation of colored state, or immobilized in b - and modified α - traps leading to mutual degradation of colored and bleached state of the films. It is found by releasing the resided ions in various traps, the initial performance can be restored accordingly. Moreover, reversible sites and all traps are demonstrated to be interconvertible through an ion trapping and detrapping process. These results provide a general scope in understanding the electrochromism in oxide thin films and offer the scheme for potential traps control or even elimination, aiming at superior electrochromic devices.
研究领域:
材料科学
·
物理化学
✓ 已验证
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2023
·
IF 5.2
Zhangyi Huang,Wanying Guo,Yang Shi,Ruichong Chen,Zhijun Wang,Yutong Zhang,Junjing Duan,Jianqi Qi,Haomin Wang
The Gd 2 Zr 2 O 7 (GZO)-based ceramic waste form exhibits promising potential for radioactive waste immobilization. However, the isomorphic immobilization capacity and irradiation effects of GZO-based ceramic waste forms with varying waste loads remain inadequately understood. This study employed a facile combustion method to synthesize ceramic powders and utilized conventional sintering with a relatively short dwelling time to fabricate GZO-based ceramic waste forms. We investigated the isomorphic immobilization behavior and He ion irradiation effects of the synthesized waste forms. The outcomes demonstrate that GZO effectively immobilizes simulated waste composed of 11 types of oxides, achieving a waste load as high as 50 wt% with a single pyrochlore phase and homogeneous element distribution. Under He + irradiation, aggravated structural degradations, including exacerbated lattice swelling, increased amorphization degree, and premature He bubble chain/microcrack formation, were observed in GZO-based waste forms with an increasing waste load.
研究领域:
材料科学
·
核能工程
·
放射化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Yuyu Liu,Minming Yan,Jia Shu,Hongwei He,Zi Wang,Ziyu Qin,Yunwei Wang,Yong Zhang
The distinctive characteristics of blue quantum dots (QDs) such as their deep valence band and large bandgap give rise to an elevated hole injection barrier between the hole transport layers (HTLs) and the QD active layer. This results in an imbalance of carrier transport and injection across the device, leading to a degrading performance in QD light-emitting diodes (QLEDs). In this paper, high-efficiency and low-efficiency degradation blue CdSe/CdS/ZnS QLEDs were fabricated by using the Lewis base, 1,2-bis(diphenylphosphino)ethane (DPPE), blended with poly(9-vinylcarbazole) (PVK) (DPPE:PVK) as HTLs. The device performance of blue QLEDs can be finely adjusted by manipulating the blending ratio between DPPE and PVK. When 4 wt % DPPE was blended with PVK (4 wt % DPPE:PVK) as the HTL, the device achieved its optimal performance. Compared to the device with neat PVK as the HTL, the turn-on voltage of blue QLEDs with the 4 wt % DPPE:PVK HTL is reduced from 3.21 to 2.9 V. The maximum current efficiency (CE) and external quantum efficiency (EQE) of blue QLEDs increase from 2.92 cd A–1 and 5.89% in neat PVK to 5.75 cd A–1 and 11.75% for the 4 wt % DPPE:PVK HTL. Furthermore, the QLEDs incorporating DPPE:PVK HTLs exhibited exceptional resistance to efficiency degradation (EQE = 8.83%@L = 12,000 cd m–2 for 4 wt % DPPE:PVK as the HTL and EQE = 2.80%@L = 12,000 cd m–2 for neat PVK as the HTL). A more in-depth analysis reveals that enhanced device performance results from the chelating and bridging effect of the bidentate ligand Lewis base DPPE. These effects strengthen the binding of free metal ions in the blue QDs, reduce the charge barriers, enhance the contact between the HTLs and the QD active layer, and ultimately improve hole injection.
研究领域:
材料科学
·
光电技术
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-14
·
IF 15.7
Zhang Hang,Li Jiayang,Liu Jinhang,Gao Yun,Fan Yameng,Liu Xiaohao,Guo Chaofei,Liu Haoxuan,Chen Xiudong,Wu Xingqiao,Liu Yang,Gu Qinfen,Li Li,Wang Jiazhao,Chou Shu-Lei
Low-cost Fe-based Prussian blue analogues often suffer from capacity degradation, resulting in continuous energy loss, impeding commercialization for practical sodium-ion batteries. The underlying cause of capacity decrease remains mysterious. Herein, we show that irreversible phase transitions, structural degradation, deactivation of surface redox centres, and dissolution of transition metal ions in Prussian blue analogues accumulate continuously during cycling. These undesirable changes are responsible for massive destruction of their morphology, leading to the capacity decay. A dual regulation strategy is applied to alleviate the above-mentioned problems of Prussian blue analogues. The designed 18650/33140 cylindrical cells using modified Prussian blue analogues and hard carbon display improved cycling stability and wide-temperature working performance (−40 o C–100 o C). The findings resolve the controversy over the origins of Prussian blue analogues cathode degradation, emphasize the necessity of eliminating irreversible crystal/structural changes to guarantee enhanced cycling stability, and demonstrate the potential of Prussian blue analogues cathode for commercial sodium-ion batteries.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）