ANALYST
·
2015
·Vol. 140
·期 5
·1572-1577
·
IF 4.107
Fen Cheng,Yue He,Xiao-Jing Xing,Dai-Di Tan,Yi Lin,Dai-Wen Pang,Hong-Wu Tang
A novel strategy for the fabrication of a colorimetric aptasensor using label free gold nanoparticles (AuNPs) is proposed in this work, and the strategy has been employed for the assay of adenosine deaminase (ADA) activity. The aptasensor consists of adenosine (AD) aptamer, AD and AuNPs. The design of the biosensor takes advantage of the special optical properties of AuNPs and the interaction between AuNPs and single-strand DNA. In the absence of ADA, the AuNPs are aggregated and are blue in color under appropriate salt concentration because of the grid structure of an AD aptamer when binding to AD, while in the presence of the analyte, AuNPs remain dispersed with red color under the same concentration of salt owing to ADA converting AD into inosine which has no affinity with the AD aptamer, thus allowing quantitative investigation of ADA activity. The present strategy is simple, cost-effective, selective and sensitive for ADA with a detection limit of 1.526 U L−1, which is about one order of magnitude lower than that previously reported. In addition, a very low concentration of the inhibitor erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) could generate a distinguishable response. Therefore, the AuNP-based colorimetric biosensor has great potential in the diagnosis of ADA-relevant diseases and drug screening.
研究领域:
生物化学
·
纳米技术
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS
·
2022
·Vol. 22
·期 9
·3430
·
IF 3.847
Xue Huang,He Li,Mengjiao Hu,Mengyuan Bai,Yemin Guo,Xia Sun
According to the chemiluminescence characteristics of the luminol-hydrogen peroxide (H2O2) system, this work designed a novel and effective electrochemiluminescence (ECL) aptasensor to detect atrazine (ATZ) rapidly. Silver nanoparticles (AgNPs) could effectively catalyze the decomposition of H2O2and enhance the ECL intensity of the luminol-H2O2system. Once ATZ was modified on the aptasensor, the ECL intensity was significantly weakened because of the specific combination between ATZ and its aptamer. Therefore, the changes in ECL intensity could be used to detect the concentration of ATZ. Under optimal detecting conditions, the aptasensor had a wide linear range from 1 × 10−3ng/mL to 1 × 103ng/mL and a low limit of detection (3.3 × 10−4ng/mL). The designed aptasensor had the advantages of good stability, reproducibility, and specificity. The aptasensor could be used to detect the ATZ content of tap water, soil, and cabbage and had satisfactory results. This work effectively constructs a novel, effective, and rapid ECL aptasensor for detecting ATZ in actual samples.Keywords:atrazine;electrochemiluminescence;aptasensor;luminol;silver nanoparticles
研究领域:
环境科学
·
分析化学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Alan Meng,Xiaocheng Hong,Yue Zhang,Jifang Yin,Liying Sheng,Zhenjiang Li
A sensitive and antifouling electrochemical aptasensor based on L-Glutamic acid (L-Glu) and peptides was constructed for the detection of adenosine triphosphate (ATP). Firstly, poly L-Glu (p-L-Glu) as the substrate material was directly grown on glass carbon electrode (GCE) via facile and controllable electrodeposition method. Subsequently, the ATP aptamer and peptides were immobilized on the modified electrode, respectively. The prepared aptasensor exhibited a wide linear range of 0.01 pM-1.0 μM, a low LOD of 0.01 pM. The aptasensor also had excellent selectivity, reproducibility and stability. Moreover, due to the synergistic effect of the p-L-Glu and peptides, the aptasensor had the same linear range towards ATP in 10% serum solutions as in ultrapure water, indicating its potential for clinical analysis.
研究领域:
分析化学
·
生物传感器
·
电化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Qiang Ma,Yuxian Wang,Jie Jia,Yuhong Xiang
Colorimetric aptasensors were designed for detection of tobramycin (TOB) based on unmodified gold nanoparticles (AuNPs) and single-strand DNA (ssDNA). In the absence of TOB, the DNA aptamer was coated on the surface of AuNPs to keep it against salt-induced aggregation. In the presence of TOB, aptamer will bind with TOB and detach from the surface of AuNPs because of higher affinities between aptamer and TOB. Then less protection of DNA may result in the aggregation of AuNPs by salt and an apparent color change from red to purple-blue. The developed aptasensors showed a high selectivity and sensitivity for TOB detection. The linearity range and the detection limit were 40–200 nM and 23.3 nM respectively. The validity of the procedure and applicability of aptasensors were successfully used to detect TOB in milk and chicken eggs, and the results were excellent in accord with the values obtained by spectrofluorimetric detection.
研究领域:
分析化学
·
纳米技术
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Kai Shi,Jiaxuan Chen,Ying Cheng,Jiuhua Song,Yang Li,Xin Cheng,Xiaofeng Bai,Jiali Chang,Tao Jiang
Microplastic (MP) pollution has attracted worldwide attention due to its harmful effects on human health and ecosystems. Herein, we develop a novel label-free electrochemical biosensor for sensitive and selective detection of MP based on split gRNA with CRISPR/Cas12a (SCas12a)-mediated cascade strand displacement and an aptamer (termed as CRISPR-MP). Within the CRISPR-MP system, the target polyvinyl chloride (PVC)/polystyrene (PS) triggers the SCas12a-mediated cascade strand displacement, and then activates the DNase activity of SCas12a. Upon cleaving the DNA probe of the G-quadruplex/hemin complex on the gold electrode, the active Cas12a triggers a decrease in the electrochemical signal.This electrochemical aptasensor shows a detection limit of 37 ng/mL of PVC and 45 ng/mL of PS, respectively. The developed CRISPR-MP system expands the utility of the CRISPR for MP detection and exhibits significant promise in protecting human health and monitoring the environment.
研究领域:
生物分析化学
·
环境监测
·
分子诊断学
·
生物传感器技术
·
纳米毒理学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Ning Li,Chenxi Ren,Qin Hu,Bo Wang,Zhenquan Yang,Lixia Xiao,Tianzhu Guan
Intake of 17β-estradiol (E2), bisphenol A (BPA), and diethylstilbestrol (DES) from food can contribute to endocrine disorders. Therefore, developing a sensitive method for the simultaneous detection of E2, BPA, and DES and understanding their combined effects on endocrine disruption are crucial. We developed a fluorescence aptasensing platform utilizing DNase I–assisted cyclic enzymatic signal amplification in conjunction with an aptamer/graphene oxide complex. Using PEG 20000 as a surface-blocking agent, the aptasensor achieved ultralow detection limits of 2.643, 0.3039, and 0.6996 for E2, BPA, and DES, respectively. The sensor demonstrated accurate detection in plastic bottled water at spiked levels of 10 and 100 ng/mL. Systems toxicology revealed 30 potential targets for mixture-induced endocrine disruption. Molecular docking showed binding affinities of E2, BPA, and DES for ESR1 of −9.94, −8.29, and − 8.98 kcal/mol, respectively. These results highlight the effectiveness of the aptasensor and provide valuable insights into endocrine disruption mechanisms.
研究领域:
分析化学
·
毒理学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 186
·期 1
·1-9
·
IF 5.705
Wang Li,Cao Hai-Xia,Pan Chang-Gang,He Yu-Sheng,Liu Hong-Fei,Zhou Li-Hong,Li Cai-Qi,Liang Guo-Xi
An aptamer-based fluorometric assay is described for the determination of bisphenol A (BPA). The aptamer against BPA is first attached to the surface of the red AuNPs, and this prevents the AuNPs from salt-induced formation of a blue-colored aggregate. Hence, the blue fluorescence of added nitrogen-doped carbon dots (NCDots) is quenched via an inner filter effect (IFE) caused by the red AuNPs. After addition of BPA, the BPA/aptamer complex is formed, and the AuNPs are no longer stabilized agains aggregation. This weakens the IFE and results in the recovery of the fluorescence of the NCDots which is measured best at excitation/emission wavelengths of 300/420 nm. The recovered fluorescence increases linearly in the 10 to 250 nM and 250 to 900 nM BPA concentration ranges, and the detection limit is 3.3 nM. The method was successfully applied to the determination of BPA in spiked environmental tap water samples.
Schematic presentation of a fluorometric aptamer based assay for bisphenol A (BPA). It is based on the inner filter effect of gold nanoparticles (AuNPs) on the fluorescence of nitrogen-doped carbon dots (NCDots).
研究领域:
分析化学
·
环境科学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Hui Feng,Meng Luo,Guonian Zhu,Kerage Dorothy Mokeira,Yaoxin Yang,Yongxin Lv,Qing Tan,Xiangwen Lei,Hang Zeng,Hefa Cheng,Shuxia Xu
This study described the development of a photocatalytic electrochemical aptasensor for the purpose of detecting chloramphenicol (CAP) antibiotic residues in water by utilizing SYBR Green I (SG) and chemically exfoliated MoS 2 (ce-MoS 2 ) as synergistically signal-amplification platforms. The Au nanoparticles (AuNPs) were electrodeposited onto the surface of an indium tin oxide (ITO) electrode. After that, the thiolate-modified cDNA, also known as capture DNA, was combined with the aptamer. Subsequently, photosensitized SG molecules and ce-MoS 2 nanomaterial were inserted into the groove of the resultant double-stranded DNA (dsDNA). The activation of the photocatalytic process upon exposure to light resulted in the generation of singlet oxygen. This singlet oxygen effectively splits the double-stranded DNA, resulting in a significant enhancement in the current of [Fe(CN) 6 ] 3-/4- . When the CAP was present, both SG molecules and ce-MoS 2 broke away from the double-stranded DNA, which turned off the photosensitization response, leading to a significant decrease in the current of [Fe(CN) 6 ] 3-/4- . Under optimal conditions, the aptasensor exhibited a linear relationship between CAP and logarithmic concentrations from 20 to 1000 nM, with a detection limit (3 σ ) of 3.391 nM. The aptasensor also demonstrated good selectivity towards CAP in the presence of interfering antibiotics such as tetracycline, streptomycin, levofloxacin, ciprofloxacin, and sulfadimethoxine. Additionally, the results obtained from the analysis of natural water samples using the proposed aptasensor were consistent with the findings acquired through the use of a liquid chromatograph-mass spectrometer. Therefore, with its simplicity and high sensitivity, this biosensor can potentially detect alternative antibiotics in environmental water samples by replacing the aptamers based on photosensitization.
研究领域:
环境科学
·
分析化学
·
生物传感器
·
水质监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Haochen Qi,Zhiwen Hu,Zhongliang Yang,Jian Zhang,Jie Jayne Wu,Cheng Cheng,Chunchang Wang,Lei Zheng
The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has lasted for almost 2 years. Stemming its spread has posed severe challenges for clinical virus detection. A long turnaround time, complicated operation, and low accuracy have become bottlenecks in developing detection techniques. Adopting a direct antigen detection strategy, we developed a fast-responding and quantitative capacitive aptasensor for ultratrace nucleocapsid protein detection based on a low-cost microelectrode array (MEA) chip. Employing the solid–liquid interface capacitance with a sensitivity of picofarad level, the tiny change on the MEA surface can be definitively detected. As a result, the limit of detection reaches an ultralow level of femtogram per milliliter in different matrices. Integrated with efficient microfluidic enrichment, the response time of this sensor from the sample to the result is shortened to 15 s, completely meeting the real-time detection demand. Moreover, the wide linear range of the sensor is from 10–5 to 10–2 ng/mL, and a high selectivity of 6369:1 is achieved. After application and evaluation in different environmental and body fluid matrices, this sensor and the detection method have proved to be a label-free, real-time, easy-to-operate, and specific strategy for SARS-CoV-2 screening and diagnosis.
研究领域:
生物医学工程
·
病毒学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Jiangxiong Zhu,Hao Yin,Yang Wang,Lumei Wang,Xueqing Geng,Yun Deng
Cadmium (Cd2+) is a highly toxic heavy metal that can accumulate in the human body through contaminated food and water, posing great health risks. In this study, a label-free fluorescent aptasensor based on SYBR Green I (SGI) for the rapid and sensitive detection of Cd2+ in food samples was designed. The aptasensor utilizes a Cd2+-specific aptamer (Cd-(21)) and its complementary strand (CSCd-(21)) to form a double-stranded DNA (dsDNA) structure in the absence of Cd2+. SGI intercalates into the dsDNA, resulting in a strong fluorescence signal. In the presence of Cd2+, the aptamer undergoes a conformational change, preventing the formation of dsDNA and leading to a decrease in fluorescence intensity. Under optimized conditions, the aptasensor exhibited a linear response to Cd2+ concentrations ranging from 0.11 to 157.37 ng mL−1, with a limit of detection (LOD) of 0.07 ng mL−1. The aptasensor demonstrated high specificity and was successfully applied to detect Cd2+ in fruits and vegetables, with satisfactory recovery rates (95–111%). The proposed aptasensor provides a promising tool for the rapid and sensitive detection of Cd2+ in food.
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Bin Lai,Haitao Wang,Wentao Su,Zhouping Wang,Bei-Wei Zhu,Chenxu Yu,Mingqian Tan
Cadmium contamination is a severe food safety risk for human health. Herein, a long afterglow “off-on” phosphorescent aptasensor was developed based on phosphorescence resonance energy transfer (PRET) for the detection of Cd 2+ in complex samples which minimizes the interference of background fluorescence. In this scheme, initially the phosphorescence of Cd 2+ -binding aptamer conjugated long afterglow nanoparticles (Zn 2 GeO 4 :Mn) was quenched by black hole quencher 1 (BHQ 1 ) modified complementary DNA. Upon encountering of Cd 2+ , the aptamer interacted with Cd 2+ and the complementary DNA with BHQ 1 was released, leading to phosphorescence recovery. The content of Cd 2+ could be quantified by the intensity of phosphorescence recovery with 100 μs gate time (which eliminated the sample autofluorescence) with a linear relationship between 0.5 and 50 μg L −1 and a limit of detection (LOD) of 0.35 μg L −1 . This method was successfully demonstrated for Cd 2+ detection in drinking water and yesso scallop samples. The “off-on” phosphorescent aptasensor based on PRET of long afterglow nanomaterials could be an effective tool for Cd 2+ detection in food samples.
研究领域:
食品科学
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2019
·Vol. 144
·期 22
·6617-6624
·
IF 4.019
Jie Gao,Yingxu Chen,Weihao Ji,Zhonghong Gao,Jingdong Zhang
A composite of CdS nanoparticles and a europium metal organic framework (Eu-MOF) (CdS/Eu-MOF) was synthesized. The unique properties of MOFs help to improve the photoelectrochemical (PEC) properties of CdS by reducing charge carrier recombination and utilizing a broader spectrum for light harvesting. Under visible light illumination, the photocurrent of the CdS/Eu-MOF composite modified electrode was about 2.5-fold higher than that of the CdS modified electrode. When an ampicillin (AMP)-binding aptamer was immobilized on the CdS/Eu-MOF modified electrode as a recognition element, a self-powered PEC aptasensor exhibiting a specific photocurrent response to AMP was constructed. Several experimental conditions such as the ratio of CdS to MOF, the coating amount of the CdS/Eu-MOF suspension and the concentration of the aptamer were studied. Under optimum conditions, the photocurrent of the developed sensor was linearly related to the logarithm AMP concentration in the range of 1 × 10−10 to 2 × 10−7 M, with a detection limit (3S/N) of 9.3 × 10−11 M. Moreover, this sensor exhibited excellent selectivity, good repeatability and desirable stability. It was successfully applied to the detection of AMP in lake water and milk samples.
研究领域:
材料科学
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）