ANALYST
·
2015
·Vol. 140
·期 5
·1572-1577
·
IF 4.107
Fen Cheng,Yue He,Xiao-Jing Xing,Dai-Di Tan,Yi Lin,Dai-Wen Pang,Hong-Wu Tang
A novel strategy for the fabrication of a colorimetric aptasensor using label free gold nanoparticles (AuNPs) is proposed in this work, and the strategy has been employed for the assay of adenosine deaminase (ADA) activity. The aptasensor consists of adenosine (AD) aptamer, AD and AuNPs. The design of the biosensor takes advantage of the special optical properties of AuNPs and the interaction between AuNPs and single-strand DNA. In the absence of ADA, the AuNPs are aggregated and are blue in color under appropriate salt concentration because of the grid structure of an AD aptamer when binding to AD, while in the presence of the analyte, AuNPs remain dispersed with red color under the same concentration of salt owing to ADA converting AD into inosine which has no affinity with the AD aptamer, thus allowing quantitative investigation of ADA activity. The present strategy is simple, cost-effective, selective and sensitive for ADA with a detection limit of 1.526 U L−1, which is about one order of magnitude lower than that previously reported. In addition, a very low concentration of the inhibitor erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) could generate a distinguishable response. Therefore, the AuNP-based colorimetric biosensor has great potential in the diagnosis of ADA-relevant diseases and drug screening.
研究领域:
生物化学
·
纳米技术
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS
·
2022
·Vol. 22
·期 9
·3430
·
IF 3.847
Xue Huang,He Li,Mengjiao Hu,Mengyuan Bai,Yemin Guo,Xia Sun
According to the chemiluminescence characteristics of the luminol-hydrogen peroxide (H2O2) system, this work designed a novel and effective electrochemiluminescence (ECL) aptasensor to detect atrazine (ATZ) rapidly. Silver nanoparticles (AgNPs) could effectively catalyze the decomposition of H2O2and enhance the ECL intensity of the luminol-H2O2system. Once ATZ was modified on the aptasensor, the ECL intensity was significantly weakened because of the specific combination between ATZ and its aptamer. Therefore, the changes in ECL intensity could be used to detect the concentration of ATZ. Under optimal detecting conditions, the aptasensor had a wide linear range from 1 × 10−3ng/mL to 1 × 103ng/mL and a low limit of detection (3.3 × 10−4ng/mL). The designed aptasensor had the advantages of good stability, reproducibility, and specificity. The aptasensor could be used to detect the ATZ content of tap water, soil, and cabbage and had satisfactory results. This work effectively constructs a novel, effective, and rapid ECL aptasensor for detecting ATZ in actual samples.Keywords:atrazine;electrochemiluminescence;aptasensor;luminol;silver nanoparticles
研究领域:
环境科学
·
分析化学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Alan Meng,Xiaocheng Hong,Yue Zhang,Jifang Yin,Liying Sheng,Zhenjiang Li
A sensitive and antifouling electrochemical aptasensor based on L-Glutamic acid (L-Glu) and peptides was constructed for the detection of adenosine triphosphate (ATP). Firstly, poly L-Glu (p-L-Glu) as the substrate material was directly grown on glass carbon electrode (GCE) via facile and controllable electrodeposition method. Subsequently, the ATP aptamer and peptides were immobilized on the modified electrode, respectively. The prepared aptasensor exhibited a wide linear range of 0.01 pM-1.0 μM, a low LOD of 0.01 pM. The aptasensor also had excellent selectivity, reproducibility and stability. Moreover, due to the synergistic effect of the p-L-Glu and peptides, the aptasensor had the same linear range towards ATP in 10% serum solutions as in ultrapure water, indicating its potential for clinical analysis.
研究领域:
分析化学
·
生物传感器
·
电化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Qiang Ma,Yuxian Wang,Jie Jia,Yuhong Xiang
Colorimetric aptasensors were designed for detection of tobramycin (TOB) based on unmodified gold nanoparticles (AuNPs) and single-strand DNA (ssDNA). In the absence of TOB, the DNA aptamer was coated on the surface of AuNPs to keep it against salt-induced aggregation. In the presence of TOB, aptamer will bind with TOB and detach from the surface of AuNPs because of higher affinities between aptamer and TOB. Then less protection of DNA may result in the aggregation of AuNPs by salt and an apparent color change from red to purple-blue. The developed aptasensors showed a high selectivity and sensitivity for TOB detection. The linearity range and the detection limit were 40–200 nM and 23.3 nM respectively. The validity of the procedure and applicability of aptasensors were successfully used to detect TOB in milk and chicken eggs, and the results were excellent in accord with the values obtained by spectrofluorimetric detection.
研究领域:
分析化学
·
纳米技术
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Xiaoting Zhao,Fei Wang,Hong Huang,Lily Chen Wang,Yanxia Qi,Qidong Wang,Yuchun Qiao,Lu Shi,Jizhen Shang,Shuai Li,Hua Wang
Herein, a highly sensitive and selective ratiometric fluorescence platform was constructed for probing adenosine triphosphate (ATP) for the first time by using magnetic Fe 3 O 4 nanozymes that were modified by ATP-specific aptamer labeled with 7-Amino-4-methyl-3-coumarinylacetic acid (Apt-AMCA) to yield Fe 3 O 4 @Apt-AMCA. It was discovered that the incorporation of Apt-AMCA greatly enhance the peroxidase-like activity of Fe 3 O 4 nanozymes, which might catalyze more o -phenylenediamine (OPD) substrates into fluorescent oxidized OPD (DAP) with a emission of 560 nm. More importantly, upon ATP addition, Apt-AMCA could potentially be released from Fe 3 O 4 @Apt-AMCA because of Apt-target binding and the competition between ATP and Apt. The rational increase in the levels of free Apt-AMCA could thereby be attained in the reaction solutions so as to induce an enhancement of fluorescence at 450 nm. Meanwhile, the desorption of Apt-AMCA from the Fe 3 O 4 @Apt-AMCA surface would decrease the peroxidase-like activity of Fe 3 O 4 @Apt-AMCA, thereby reducing its ability to catalyze OPD into fluorescent DAP and thus decreasing fluorescence at 560 nm. A ratiometric fluorescence method was accordingly formulated to permit the sensitive and selective identification of ATP, featuring a linear dynamic range that ranges from 0.10 μM to 80 μM. Accurate measurements of ATP in practical blood samples were also confirmed, promising a new detection method for ATP analysis that can widely applied in clinical laboratories.
研究领域:
分析化学
·
纳米技术
·
生物化学
·
传感器
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Kaiyan Fan,Junlun Zhu,Xiaowei Wu,Xiuhua Zhang,Shengfu Wang,Wei Wen
In this work, a flexible and label-free electrochemical biosensing platform had been developed based on split aptamers and the cleavage effect of exonuclease I, which was applied for the detection of different targets in parallel. In the presence of targets, the two split aptamers could specifically recognize targets to form a ternary complex, which cannot be digested by exonuclease I, resulting in significant impedance signal output. The proposed label-free aptasensor realized sensitive and selective detection of thrombin, adenosine triphosphate and mercury ion with the detection limits of 32 fM, 1.43 pM and 1.65 pM, respectively. The designed strategy provided a simple and powerful tool for multiple targets detection, holding promising applications in disease diagnosis and environmental monitoring.
研究领域:
生物传感器
·
电化学
·
分析化学
·
环境监测
·
疾病诊断
✓ 已验证
产品关联
DOI
高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 6
·387
·
IF 5.743
Sen Li,Defu He,Shuning Li,Ruipeng Chen,Yuan Peng,Shuang Li,Dianpeng Han,Yu Wang,Kang Qin,Shuyue Ren,Ping Chen,Zhixian Gao
Bisphenol A (BPA) has emerged as a contaminant of concern because long-term exposure may affect the human endocrine system. Herein, a novel aptamer sensor based on magnetic separation and surface-enhanced Raman scattering (SERS) is proposed for the extremely sensitive and specific detection of trace BPA. Moreover, the capture unit was prepared by immobilizing thiolated (SH)-BPA aptamer complementary DNA on AuNP-coated magnetic halloysite nanotubes ([email protected]), and SH-BPA aptamer-modified[email protected]@Ag core–shell SERS nanotags acted as signal units. By the complementary pairing of the BPA aptamer and the corresponding DNA,[email protected]and[email protected]@AgCS were linked together through hybridization-ligation, which acted as the SERS substrate. In the absence of BPA, the constructed aptamer sensor generated electromagnetic enhancement and plasmon coupling to improve the sensitivity of SERS substrates. Owing to the high affinity between BPA and the aptamer, the aptamer probe bound to BPA was separated from the capture unit by an externally-induced magnetic field. Thus, the Raman intensity of the[email protected]@AuCS core–satellite assemblies was negatively correlated with the BPA concentration. High sensitivity measurements of BPA might be performed by determining the decline in SERS signal strength together with concentration variations. The proposed aptasensor is a promising biosensing platform for BPA detection.Keywords:surface-enhanced Raman scattering (SERS);aptasensor;bisphenol A;core–satellite assemblies
研究领域:
分析化学
·
纳米技术
·
环境科学
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Ning Li,Chenxi Ren,Qin Hu,Bo Wang,Zhenquan Yang,Lixia Xiao,Tianzhu Guan
Intake of 17β-estradiol (E2), bisphenol A (BPA), and diethylstilbestrol (DES) from food can contribute to endocrine disorders. Therefore, developing a sensitive method for the simultaneous detection of E2, BPA, and DES and understanding their combined effects on endocrine disruption are crucial. We developed a fluorescence aptasensing platform utilizing DNase I–assisted cyclic enzymatic signal amplification in conjunction with an aptamer/graphene oxide complex. Using PEG 20000 as a surface-blocking agent, the aptasensor achieved ultralow detection limits of 2.643, 0.3039, and 0.6996 for E2, BPA, and DES, respectively. The sensor demonstrated accurate detection in plastic bottled water at spiked levels of 10 and 100 ng/mL. Systems toxicology revealed 30 potential targets for mixture-induced endocrine disruption. Molecular docking showed binding affinities of E2, BPA, and DES for ESR1 of −9.94, −8.29, and − 8.98 kcal/mol, respectively. These results highlight the effectiveness of the aptasensor and provide valuable insights into endocrine disruption mechanisms.
研究领域:
分析化学
·
毒理学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yi Zhang,Jing Cao,Jie Zhou,Wen-Long Wang,Zhenghua Xu,Yong-Wei Feng,Xiao-Dong Huang,Feng-De Yu,Xin Yang
Patulin (PAT) is a fungal toxin often found in fruit and juice products. The sensitive and fast detection of PAT can help the control of PAT-induced health risk. However, the lack of efficient and stable antibody for PAT hindered the advancement of its test strips. Here, we constructed a nucleic acid-based lateral flow test strip (NALFTS) and used photothermal-colorimetric dual mode for the detection of PAT. The binding of PAT in samples to the aptamer on magnetic separation probe MNPs-Apt@cDNA induced the release of cDNA competitively. Subsequently, cDNA initiates strand displacement amplification (SDA), generating numerous single-strand DNA (ssDNA). These ssDNA molecules were analyzed by a test strip with photothermal-colorimetric dual signal. The developed method demonstrated a limit of detection (LOD) of 5 pg mL −1 and a quantitative range of 0.01 ∼ 320 ng mL −1 in photothermal mode, and a LOD of 0.1 ng mL −1 and a quantitative range of 0.1 ∼ 40 ng mL −1 in colorimetric mode. This SDA-NALFTS offered dual signals corroborating each other to provide more reliable results for rapid screening or accurate monitoring of PAT in juice.
研究领域:
食品安全检测
·
分子诊断学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Kai Shi,Jiaxuan Chen,Ying Cheng,Jiuhua Song,Yang Li,Xin Cheng,Xiaofeng Bai,Jiali Chang,Tao Jiang
Microplastic (MP) pollution has attracted worldwide attention due to its harmful effects on human health and ecosystems. Herein, we develop a novel label-free electrochemical biosensor for sensitive and selective detection of MP based on split gRNA with CRISPR/Cas12a (SCas12a)-mediated cascade strand displacement and an aptamer (termed as CRISPR-MP). Within the CRISPR-MP system, the target polyvinyl chloride (PVC)/polystyrene (PS) triggers the SCas12a-mediated cascade strand displacement, and then activates the DNase activity of SCas12a. Upon cleaving the DNA probe of the G-quadruplex/hemin complex on the gold electrode, the active Cas12a triggers a decrease in the electrochemical signal.This electrochemical aptasensor shows a detection limit of 37 ng/mL of PVC and 45 ng/mL of PS, respectively. The developed CRISPR-MP system expands the utility of the CRISPR for MP detection and exhibits significant promise in protecting human health and monitoring the environment.
研究领域:
生物分析化学
·
环境监测
·
分子诊断学
·
生物传感器技术
·
纳米毒理学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Limin Yang,Yiping Zhang,Xingdong Dong,Xuan Wang,Mingming Li,Lei Jiang,Zhen Liu
For electrochemical aptamer analysis based on magnetic beads (MBs), especially for electrochemical impedance analysis, MBs are often directly involved in signal measurements, which may cause high background signals, as well as interference from non-specific adsorption. Herein, a method for the indirect involvement of MBs in impedance aptasensing was developed. The key to this method is the construction of a Strep-Tag system integrated apta-magnetic probe, in which the Strep-tag system was employed as a link between the aptamer and MBs. The acetamiprid-binding aptamer (ABA) was indirectly attached to Strep-Tactin-coated MBs (STMBs), through the hybridization with its complementary strand (cABA), which was covalently conjugated to Strep-tag II. After acetamiprid capture, desthiobiotin triggered the release of Strep-tag II-conjugated cABA, which was subsequently interacted with the ABA-modified electrode for impedance measurements. Moreover, streptavidin-conjugated horseradish peroxidase (streptavidin-HRP) was added to amplify the impedance signal. The changes in electron-transfer resistance (Δ R ct ) are linearly related to the logarithm of acetamiprid concentration in the range of 0.5–10 pM. Detection limit as low as 0.15 pM was achieved. Satisfactory recoveries ranging from 95.25 to 101.2 % with relative standard deviations of 5.12 % were also obtained in analyzing real spiked agricultural products.
研究领域:
分析化学
·
电化学
·
生物传感器
·
农业化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 186
·期 1
·1-9
·
IF 5.705
Wang Li,Cao Hai-Xia,Pan Chang-Gang,He Yu-Sheng,Liu Hong-Fei,Zhou Li-Hong,Li Cai-Qi,Liang Guo-Xi
An aptamer-based fluorometric assay is described for the determination of bisphenol A (BPA). The aptamer against BPA is first attached to the surface of the red AuNPs, and this prevents the AuNPs from salt-induced formation of a blue-colored aggregate. Hence, the blue fluorescence of added nitrogen-doped carbon dots (NCDots) is quenched via an inner filter effect (IFE) caused by the red AuNPs. After addition of BPA, the BPA/aptamer complex is formed, and the AuNPs are no longer stabilized agains aggregation. This weakens the IFE and results in the recovery of the fluorescence of the NCDots which is measured best at excitation/emission wavelengths of 300/420 nm. The recovered fluorescence increases linearly in the 10 to 250 nM and 250 to 900 nM BPA concentration ranges, and the detection limit is 3.3 nM. The method was successfully applied to the determination of BPA in spiked environmental tap water samples.
Schematic presentation of a fluorometric aptamer based assay for bisphenol A (BPA). It is based on the inner filter effect of gold nanoparticles (AuNPs) on the fluorescence of nitrogen-doped carbon dots (NCDots).
研究领域:
分析化学
·
环境科学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）