ANALYST
·
2023
·Vol. 148
·期 11
·2459-2464
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IF 4.2
Qianying Wang,Junyi Zhang,Ruo Yuan,Yun Xiang
The convenient and sensitive assay of antibiotic residuals is important for monitoring food safety and human health. Here, with a new self-constrained metal ion-dependent DNAzyme and hybridization chain reaction (HCR) signal amplifications, a simple label-free and highly sensitive aptamer-based fluorescent sensing assay for sarafloxacin is established. The target sarafloxacin molecules bind the aptamers in the duplex DNA probes to release the complementary strands, which cyclically activate the catalytic activity of the self-constrained DNAzymes to cut the substrate sequences to liberate many ssDNA strands. Subsequent HCR of two hairpins is initiated by these ssDNA strands for yielding long dsDNAs with lots of G-quadruplexes, which intercalate thioflavin T dye to generate drastically magnified fluorescence for non-label and sensitive sarafloxacin detection at the detection limit of 29 pM. Also, a highly selective assay of low levels of sarafloxacin in diluted milk samples has been demonstrated, showing the great potential of our approach for the development of versatile sensitive and convenient aptasensors for monitoring different antibiotics.
研究领域:
分析化学
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生物化学
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食品科学
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生物技术
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DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2022
·
IF 5.76
Sasan Radfar,Reza Ghanbari,Ali Attaripour Isfahani,Halimeh Rezaei,Majid Kheirollahi
Determination of microRNAs (miRNAs) as valuable blood-borne biomarkers has attracted many scientific attentions. However, analytical methods are still restricted by miRNAs intrinsic characteristics. In this study, for the first time, novel blackberry-like magnetic DNA/FMMA nanospheres were synthesized and mounted on a gold stir-bar as signal amplification probes. To produce this strong electrochemical signal label, double strand DNAs were immobilized on gold coated magnetic nanospheres through a hybridization chain reaction followed by reversible addition-fragmentation chain-transfer polymerization, which brought a great quantity of the electroactive tags (FMMA) on the nanosphere surface. These nanospheres were then fixed on the gold stir-bar as signal probes. The magnetic DNA/FMMA nanosphere probes can be released by substituting with the newly emerging DNA fragments of catalyzed hairpin assembly products. Eventually, these signal probes were magnetically enriched on the electrode surface to produce electrochemical signal and finally, the biosensor was developed to detect miRNA-106a (model target). The suggested aptamer-based biosensor demonstrated considerable selectivity, acceptable storage stability, high specificity, and excellent performance in real sample analysis without any pretreatments. As a result, current study reveals that the developed strategy has a great potential for the early diagnosis of gastric cancer and additionally the clinical monitoring of any miRNA sequences.
研究领域:
生物化学
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纳米技术
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生物医学工程
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癌症研究
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分子生物学
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DOI
PubMed
高影响因子
ACS Sensors
·
2024
·
IF 8.2
Jiping Ni,Huan Wei,Wenliang Ji,Yifei Xue,Fenghui Zhu,Chunxia Wang,Ying Jiang,Lanqun Mao
Selective and nondisruptive in vivo neurochemical monitoring within the central nervous system has long been a challenging endeavor. We introduce a new sensing approach that integrates neurocompatible galvanic redox potentiometry (GRP) with customizable phosphorothioate aptamers to specifically probe dopamine (DA) dynamics in live rat brains. The aptamer-functionalized GRP (aptGRP) sensor demonstrates nanomolar sensitivity and over a 10-fold selectivity for DA, even amidst physiological levels of major interfering species. Notably, conventional sensors without the aptamer modification exhibit negligible reactivity to DA concentrations exceeding 20 μM. Critically, the aptGRP sensor operates without altering neuronal activity, thereby permitting real-time, concurrent recordings of both DA flux and electrical signaling in vivo. This breakthrough establishes aptGRP as a viable and promising framework for the development of high-fidelity sensors, offering novel insights into neurotransmission dynamics in a live setting.
研究领域:
神经科学
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生物医学工程
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分析化学
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生物技术
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DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Haijiao Gan,Hui Xu
In this work, an innovative aptamer-based magnetic adsorbent (Fe 3 O 4 @PDA-aptamer MNPs) was prepared by hydrothermal synthesis method followed by the surface functionalization of nanoparticles . After fixing in a steel stainless tube as sorbent of magnetic solid phase extraction (MSPE), an online magnetic solid phase extraction-high performance liquid chromatography-mass spectrometry (online-MSPE-HPLC-MS) method was developed and applied for the determination of 8-hydroxy-2′-deoxyguanosine (8-OHdG) and monohydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) simultaneously in urine. The synthesized sorbent presented outstanding features, including large specific surface area, high enrichment capacity and excellent stability. High throughput analysis can be achieved by affinity-specific adsorption of 8-OHdG and non-specific adsorption of OH-PAHs at the same time. In addition, online MSPE can greatly simplify the analysis process, reduce human errors and enhance the sensitivity. When compared with offline MSPE, a sensitivity enhancement of 30–400 times was obtained for the online method. Some experimental parameters such as the amount of the sorbent, sampling flow rate and sample volume, were optimized systematically. Under the optimal conditions, the limits of detection (LOD) were in the range of 0.028–0.114 ng mL −1 , and the correlation coefficients (R 2 ) were higher than 0.9962. The relative standard deviations (RSDs) were less than 16.1% (n = 5) and the recoveries ranged from 71% to 116%. The above results show that the rapid, sensitive and automated online-MSPE-HPLC-MS method has potential application in the simultaneous determination of 8-OHdG and PAHs in complex sample matrix to assess the environmental exposure level.
研究领域:
分析化学
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环境科学
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生物化学
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DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 45
·35448-35452
·
IF 3.84
Xuejuan Chen,Xiaojing Bai,Huiyan Li,Bailin Zhang
An aptamer-based microcantilever array sensor for detection of fumonisin B-1 (FB1) was developed. The sensing cantilevers in the array were functionalized with self-assembled monolayers (SAMs) of thiolated FB1-specific aptamer, while the reference cantilevers were modified with 6-mercapto-1-hexanol SAMs to eliminate interference from the environment by detecting the deflection induced by nonspecific interactions. This differential deflection amplitude between sensing and reference cantilevers shows a linearity relation with the concentration of FB1 in the range from 0.1 to 40 μg mL−1, with a limit of detection of 33 ng mL−1 (S/N = 3). The sensor exhibits good specificity over ochratoxin A (OTA) and deoxynivalenol (DON). This biosensor provides a promising approach to detect FB1 in food and agricultural products.
研究领域:
生物传感器
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食品安全
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分析化学
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毒理学
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DOI
高影响因子
危险品化学品经营许可证（带存储）