SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Kaiwu Zhang,Xuan Wang,Caicheng Long,Jin Xu,Zixin Jiang,Bo Feng,Peng Zhang,Junjie Fei,Taiping Qing
DNA templated fluorescent copper nanoclusters (DNA-CuNCs) have demonstrated enormous potential for biochemical analysis. However, most of them are focused on DNA-related sensing. Here, we designed a hairpin DNA/RNA chimeric chain for the formation of fluorescent CuNCs and further applied it to detect reverse transcription-associated ribonuclease H (RNase H). The DNA/RNA chimera templated copper nanoclusters (DNA/RNA-CuNCs) exhibited similar spectral properties with DNA-CuNCs. In the presence of RNase H, the RNA segment in chimera is hydrolyzed and release short oligonucleotides, which fail to synthsize fluorescent CuNCs. The capabilities for RNase H detection from complex samples and screening of inhibitors were verified. This strategy exhibited high sensitivity for RNase H with a detectable minimum concentration of 5.5 × 10 −4 U mL -1 , which is lower than the developed fluorescent methods. This work will open up new thoughts to design new template for metal nanoclusters and broaden their application in RNA-related sensing.
研究领域:
生物化学
·
分子生物学
·
分析化学
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DOI
高影响因子
Food & Function
·
2021
·Vol. 12
·期 7
·2914-2924
·
IF 5.396
Li Tao,Wei Zhang,Yaxin Zhang,Mei Zhang,Yueying Zhang,Xing Niu,Qing Zhao,Zhenxing Liu,Yuyin Li,Aipo Diao
Telomere shortening is one of the main causes of cellular senescence. Caffeine is a natural stimulant most commonly found in coffee and tea. In this study, caffeine was found to promote the expression of telomerase reverse transcriptase (TERT) at both mRNA and protein levels, and consequently extended the telomere length and prevented cellular senescence. Knockdown of TERT eliminated the effect of caffeine on telomere elongation. Moreover, animal studies indicated that caffeine promoted the expression of TERT and extended the telomere length in the thymus and spleen of mice treated with caffeine for a long period of eight months. In addition, caffeine restored the decline of organ index and improved the histological structural change of the thymus, spleen and liver of mice due to aging. These results suggest that caffeine promotes the expression of TERT to delay cellular senescence and aging, which help to understand the mechanism for the beneficial effects of caffeine containing foods on health.
研究领域:
分子生物学
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老年学
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营养学
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细胞生物学
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DOI
PubMed
高影响因子
ENVIRONMENT INTERNATIONAL
·
2019
·
IF 7.943
Chengzhi Zhou,Yanping Wang,Jie Chen,Junfeng Niu
Electrochemical degradation of trace antiretroviral drug stavudine was investigated by using a reactive electrochemical membrane (REM) with Ti/SnO 2 -Sb anode. From the results it was evident that the stavudine degradation followed pseudo-first-order kinetics, with the values of the degradation rate constant and half-life being 0.24 min −1 and 2.9 min, respectively, at a current density of 8 mA cm −2 . The degradation rate was obviously decreased under alkaline condition (pH = 11.0) and the degradation was also inhibited in the presence of NO 3 − and Cl − . Five intermediates were identified in the electrochemical degradation of stavudine, and the degradation pathways were proposed. Density functional theory calculation revealed that the double bond carbon atom nearby hydroxymethyl group was the site attacked by OH and the cleavage of C N bond was the rate-determining step in the electrochemical degradation of stavudine. The nitrogen in stavudine was mainly converted to nitrate and ammonium. Quantitative structure-activity relationship model indicated that the toxicity of some intermediates was higher than the parent compound stavudine. The electric energy consumption for 90% stavudine degradation ranged from 0.87 to 2.29 Wh L −1 at the experimental conditions, indicating that stavudine can be degraded efficiently by the REM with Ti/SnO 2 -Sb anode.
研究领域:
环境科学
·
电化学工程
·
药物化学
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DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Andere Clement Miruka,Xiaoting Gao,Yinyin Zhang,Samuel A. Iwarere,Pengcheng Luo,Austin O. Aluoch,Geoffrey Otieno,Han Zhang,Yanan Liu
Antiretroviral drugs (ARVDs), used in the treatment of human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) have been recognised as emerging contaminants (ECs). Various ARVDs have been identified and quantified in surface waters worldwide. This research presents the first degradation of efavirenz using dielectric barrier discharge (DBD) plasma. Efavirenz was successfully degraded with 93 % removal efficiency at discharge power of 59.3 W and pH 9. Incorporating free radical promoters (Fe 2+ /H 2 O 2 ) significantly improved the rate constants from 0.134 min −1 to 0.269 min −1 resulting in a 40 % increment in energy yield, from 235 mg/kWh to 330.5 mg/kWh. The presence of free radical promoters also eliminated the need for upward pH adjustments. The optimal gas flow rate of 0.6 L/min enabled maximized production of reactive species that resulted to highest efavirenz removal efficiency (93 %). Electron paramagnetic resonance (EPR) confirmed the presence of •OH and ONOO - radical species during plasma discharge. Subsequent chemical probes confirmed the dominance of e - aq and •OH in efavirenz degradation. Coexisting inorganic ions largely retarded efavirenz degradation. Six degradation by-products were identified, and possible degradation pathway was proposed. Electron transfer and hydrogen abstraction were the primary mechanisms through which the radicals attacked the efavirenz contaminant. DBD demonstrated adaptability to actual water matrices contaminated with efavirenz, signifying its practical feasibility as a treatment option for ARVD-contaminated waters in the environment. This study provides an invaluable reference for understanding the degradation of ARVDs in the environment using DBD plasma, contributing to the collective endeavours aimed at preserving the integrity of terrestrial and aquatic ecosystems .
研究领域:
环境科学
·
药学
·
等离子体技术
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Andere Clement Miruka,Xiaoting Gao,Li Cai,Yinyin Zhang,Pengcheng Luo,Geoffrey Otieno,Han Zhang,Zhiqi Song,Yanan Liu
The global prevalence of human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) has been an environmental menace. Tons of drug wastes from antiretroviral therapy are released into the environment annually. We, for the first time, employed the novel dielectric barrier atmospheric non-thermal plasma (DBANP) discharge, to mitigate the inadvertent pollution arising from the antiretroviral therapy. A 40-min treatment of nevirapine achieved >94 % (0.075 min −1 ) removal efficiency at discharge power of 63.5 W and plasma working gas of atmospheric air. Chemical probes confirmed •OH, ONOO − and e aq − as the dominant reactive species whilst further revealing the reaction acceleration role of NaNO 3 and CCl 4 which are known reaction terminators. The commonly coexisting inorganic anions potentiated nevirapine removal with over 98 % efficiency, achieving the highest rate constant of 0.148 min −1 in this study. Moreover, the initial solution pH (1.5–11.1) was no limiting factor either. The insensitivity of the DBANP discharge to actual water matrices was an eminent inference of its potential applicability in practical conditions. With reference to data obtained from the liquid chromatography-mass spectrometer analysis, nevirapine degradation pathway was proposed. A nucleophilic attack by ONOO − at the cyclopropyl group and •OH attack at the carbonyl carbon of the amide group, respectively, initiated nevirapine degradation process. It is anticipated that the findings herein, will provide new insights into antiretroviral drug waste management in environmental waters using the innovative and green non-thermal plasma process.
研究领域:
环境科学
·
药学
·
等离子体技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF INFECTIOUS DISEASES
·
2008
·Vol. 197
·期 1
·117-125
·
IF 6.035
Michaëlsson Jakob,Long Brian R.,Loo Christopher P.,Lanier Lewis L.,Spotts Gerald,Hecht Frederick M.,Nixon Douglas F.
Natural killer (NK) cells are believed to play a role in human immunodeficiency virus type 1 (HIV-1) disease progression, and NK cell levels are reduced in individuals with chronic HIV-1 infection. Interleukin (IL)-2 therapy results in an expansion of CD4+ T cells as well as NK cells; however, little is known about the detailed effects of IL-2 therapy on NKcells in HIV-1 infection in general and in early infection in particular. Here, we investigated the effects of combined IL-2 therapy and antiretroviral therapy (ART) on the number, frequency, phenotype, and interferon (IFN)-γ production of NK cells in individuals with early HIV-1 infection. Patients randomized to receive combined ART and IL-2 therapy predominantly expanded CD56dim NK cells, and the expansion was greater than in patients randomized to receive ART alone. Importantly, NK cell receptor expression and IFN-γ production were maintained over time. This reconstitution ofNKcells may be useful in helping contain viremia if patients discontinue therapy or develop drug resistance.
研究领域:
免疫学
·
病毒学
·
传染病学
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DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
·
2020
·
IF 3.263
Yanzheng Xie,Lei Xie,Ailan Chen,Shengnan Wu,Yuxiao Mo,Xiaoqiang Guo,Changchun Zeng,Xinan Huang,Jinyang He
AIDS , a serious fatal disease caused by the human immunodeficiency virus (HIV), is an epidemic disease for which no effective vaccine has been established. The current therapeutic interventions for AIDS have limited efficacy because they are unable to clear HIV infections and the continuous occurrence of resistant HIV strains. Therefore, the exploitation of new drugs to prevent the spread of AIDS remains a high priority. In this study, the effects of icariin and its metabolite anhydroicaritin on SIV/HIV replication were investigated. In CEM × 174 cells and PBMC cells, both icariin and anhydroicaritin can significantly inhibit HIV-1 or SIVmac251 replication. Furthermore, molecular docking studies revealed that icariin and anhydroicaritin can act on both HIV reverse transcriptase and protease but could not bind to integrase . Reverse transcriptase and protease inhibition biological assays showed that both icariin and anhydroicaritin could significantly inhibit only HIV reverse transcriptase. In summary, the two compounds can significantly inhibit HIV/SIV in vitro and their targets may be mainly involved with HIV reverse transcriptase.
研究领域:
病毒学
·
药理学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Communications Biology
·
2023
·Vol. 6
·期 1
·1-12
·
IF 5.9
Wang You-Yuan,Zhen Cheng,Hu Wei,Huang Hui-Huang,Li Yan-Jun,Zhou Ming-Ju,Li Jing,Fu Yu-Long,Zhang Peng,Li Xiao-Yu,Yang Tao,Song Jin-Wen,Fan Xing,Zou Jun,Meng Si-Run,Qin Ya-Qin,Jiao Yan-Mei,Xu Ruonan,Zhang Ji-Yuan,Zhou Chun-Bao,Yuan Jin-Hong,Huang Lei,Shi Ming,Cheng Liang,Wang Fu-Sheng,Zhang Chao
CD8 + T cells are essential for long-lasting HIV-1 control and have been harnessed to develop therapeutic and preventive approaches for people living with HIV-1 (PLWH). HIV-1 infection induces marked metabolic alterations. However, it is unclear whether these changes affect the anti-HIV function of CD8 + T cells. Here, we show that PLWH exhibit higher levels of plasma glutamate than healthy controls. In PLWH, glutamate levels positively correlate with HIV-1 reservoir and negatively correlate with the anti-HIV function of CD8 + T cells. Single-cell metabolic modeling reveals glutamate metabolism is surprisingly robust in virtual memory CD8 + T cells (TVM). We further confirmed that glutamate inhibits TVM cells function via the mTORC1 pathway in vitro. Our findings reveal an association between metabolic plasticity and CD8 + T cell-mediated HIV control, suggesting that glutamate metabolism can be exploited as a therapeutic target for the reversion of anti-HIV CD8 + T cell function in PLWH.
研究领域:
免疫学
·
病毒学
·
代谢学
·
HIV/AIDS研究
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产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Yaqin Zhang,Jianai Chen,Fange Kong,Chunxia Wang,Hangyu Guo,Yingchun Li,Jiahui Lu,Jicheng Zhang,Jiasi Wang,Yulin Zhou
Viral infections, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), are some of the most dangerous threats to humans. SARS-CoV-2 has caused a global pandemic, highlighting the unprecedented demand for rapid and portable diagnostic methods. To meet these requirements, we designed a label-free colorimetric platform that combines the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated proteins (Cas) 12a system for naked-eye detection (named LFP). This method utilizes reverse transcription loop-mediated isothermal amplification (RT-LAMP) and the trans-cleavage activity of the CRISPR/Cas12a system to increase the sensitivity and specificity of the reaction. This platform can detect as few as 4 copies/μL of RNA and produces no false positive results when tested against the influenza virus. To better meet the requirements of point-of-care (POC) detection, we developed a portable device that can be applied in resource-poor and densely populated regions. The LFP assay holds great potential for application in resource-limited settings, and the label-free gold nanoparticle (AuNPs) probe can reduce costs, making it suitable for large-scale screening. We expect that the LFP assay will be promising for the POC screening of COVID-19.
研究领域:
分子生物学
·
生物技术
·
病毒学
·
纳米技术
·
诊断方法
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产品关联
DOI
PubMed
高影响因子
Zebrafish
·
2023
·Vol. 20
·期 2
·55-66
·
IF 2
Hui Xia,Xue Cheng,Mengxi Cao,Xiongjie Sun,Fuyi He,Xiaowei Yao,Hongtao Liu
Population aging is a global problem worldwide, and the discovery of antiaging drugs and knowledge of their potential molecular mechanisms are research hotspots in biomedical field. Tetrahydroxystilbene glucoside (TSG) is a natural component isolated from Heshouwu (Polygonum multiflorum Thunb.). It has been widely used to treat various chronic diseases for its remarkable biological activities. In this study, we successfully established aging larval zebrafish by exposing larvae to 2 mM hydrogen peroxide (H2O2). Using this aging model, we assessed the antiaging effect of TSG with different concentrations (25–100 μg/mL). After being treated with H2O2, zebrafish showed the obvious aging-associated phenotypes characterized by higher senescence-associated β-galactosidase activity, significantly downregulated expression of sirtuin 1 (sirt1) and telomerase reverse transcriptase (tert), and upregulated serpine1 mRNA level compared to the control group. TSG pretreatment delayed the aging process of oxidative stress-induced zebrafish, indicative of the reduced positive rate of senescence-associated β-galactosidase, improved swimming velocity, and stimulus-response capacity. Further studies proved that TSG could suppress reactive oxygen species production and enhance the activity of antioxidant enzymes superoxide dismutase and catalase. TSG also inhibited the H2O2-induced expressions of inflammation-related genes il-1β, il-6, cxcl-c1c, and il-8 in aging zebrafish, but it did not affect apoptosis-related genes (bcl-2, bax, and caspase-3) of aging zebrafish. In conclusion, TSG can protect against aging by regulating the antioxidative genes and enzyme activity, as well as inflammation in larval zebrafish, providing insight into the application of TSG for clinical treatment of aging or aging-related diseases.
研究领域:
药理学
·
老年学
·
分子生物学
·
神经生物学
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DOI
PubMed
高影响因子
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
·
2023
·
IF 2.1
Ke Shenghui,Feng Yiwei,Luo Liying,Qin Wanzhao,Liu Huayu,Nie Jingchong,Liang Beijiang,Ma Hongjie,Xie Mao,Li Jingyu,Niu Zhijie,Li Guojian,Tang Anzhou,Xia Wei,He Guangyao
A method for the in vitro isolation, purification, identification, and induced differentiation of satellite cells from adult tree shrew skeletal muscle was established. The mixed enzyme digestion method and differential adhesion method were used to obtain skeletal muscle satellite cells, which were identified and induced to differentiate to verify their pluripotency. The use of a mixture of collagenase II, hyaluronidase IV, and DNase I is an efficient method for isolating adult tree shrew skeletal muscle satellite cells. The P3 generation of cells had good morphology, rapid proliferation, high viability, and an “S”-shaped growth curve. Reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescence staining indicated that marker genes or proteins were expressed in skeletal muscle satellite cells. After myogenic differentiation was induced, multiple-nucleated myotubes were observed, and the MyHC protein was expressed. The expression of myogenic marker genes changed with the differentiation process. After the induction of adipogenic differentiation, orange-red lipid droplets were observed, and the expression of adipogenic marker genes increased gradually with the differentiation process. In summary, satellite cells from adult tree shrew skeletal muscle were successfully isolated using a mixed enzyme digestion method, and their potential for differentiation into myogenic and adipogenic cells was confirmed, laying a foundation for further in vitro study of tree shrew muscle damage.
研究领域:
细胞生物学
·
肌肉生理学
·
干细胞研究
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产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2024
·
IF 14.3
Lijiao He,Xiao Zhang,Shengwei Zhang,Yi Wang,Weichao Hu,Jie Li,Yunyi Liu,Yu Liao,Xue Peng,Jianjun Li,Haiyan Zhao,Liting Wang,Yang-Fan Lv,Chang-Jiang Hu,Shi-Ming Yang
Persistent H. pylori infection triggers the repair program of the mucosa, such as spasmolytic polypeptide-expressing metaplasia (SPEM). However, the mechanism underlying the initiation of SPEM in gastric tissues by H. pylori remains unclear. Here, an increase in telomerase reverse transcriptase (TERT) protein expression is observed in chief cells upon infection with cagA -positive H. pylori . Tert knockout significantly ameliorated H. pylori -induced SPEM and single-cell RNA sequencing demonstrated that the Wnt/β-Catenin pathway is suppressed in gastric cells with Tert knockout. Mechanism study revealed that CagA elevated TERT abundance by disrupting the interaction between TERT and its novel E3 ligase, SYVN1. Interestingly, Nitazoxanide effectively relieved SPEM via inhibition of the Wnt/β-Catenin signaling in vivo. This results clarified the mechanism underlying which CagA activated the TERT/Wnt/β-Catenin pathway, thus promoting the dedifferentiation of chief cells and the occurrence of SPEM in gastric mucosa. This highlights a molecular basis for targeting CagA-activated Wnt signaling in chief cells for the treatment of gastric precancerous lesions.
研究领域:
胃肠病学
·
分子生物学
·
肿瘤学
·
病理学
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DOI
PubMed
高影响因子
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