BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Long Huang,Liuyi Zhao,Zhuowen Wang,Zhiqiang Chen,Shengyong Jia,Yali Song
Polyhydroxyalkanoate (PHA) producer selection is a key step in mixed culture (MC) production. This study focused on incompatibility between PHA storage and floc settling of MCs in the selection process. In a selector using fermented waste activated sludge as substrate under varying organic loading, average maximum PHA content obtained in batch assays increased by ∼ 22 wt% and biomass concentration increased by ∼ 34% with the increasing of organic loading. However, poor floc settling occurred, causing decreased batch PHA production and costly downstream process. A flank community which can corporately use non-VFA organics existed in the selector. When organic loading increased, PHA producers had stronger negative interactions, but not cooperation with the flank community members. Thus, high PHA storage of MCs was bounded to the domination of core PHA producer. But the domination of Thauera bacteria under high organic loading indirectly induced a bloom of filamentous bacteria .
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2016
·
IF 3.926
Shanshan Wang,Chao Han,Sisi Wang,Lijuan Bai,Shanshan Li,Jianguang Luo,Lingyi Kong
Cu(II) complexed amino acid ionic liquid , Cu(II)-[1-butyl-3-methylimidazolium][L-Pro] (Cu(II)-[BMIm][L-Pro]), was successfully adopted as chiral ligand to improve the enantioseparation efficiency in high speed counter-current chromatography (HSCCC). For the enantioseparation of intractable naringenin (NRG) racemic mixtures, Cu(II)-[BMIm][L-Pro] coupled with hydroxypropyl-β-cyclodextrin (HP-β-CD) was successfully applied as dual chiral selectors in HSCCC. The influence of important parameters, including the concentration of the chiral selectors, the pH value, and the temperature were investigated. Under optimal conditions, 4.5 mg of (+)-NRG and 4.1 mg of (−)-NRG were successfully separated from 10 mg NRG racemic mixtures with the purity of 98%. The chiral recognition mechanism of dual chiral selectors was illuminated by the UV–vis and NMR spectra, suggesting that the enantioseparation was upon the difference of the thermodynamic stability of the quaternary complexes of Cu(II), [BMIm][L-Pro], HP-β-CD, and NRG. The results illustrated that the developed HSCCC system, based on the synergistic mechanism of Cu(II)-[BMIm][L-Pro] and HP-β-CD, exhibited better performance on enantioseparation and had great application potential in preparative chiral separation of natural products.
研究领域:
分析化学
·
药物化学
·
天然产物研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 20
·4907-4914
·
IF 3.864
Yufeng Zhou,Yali Yu,Longlong Gao,Yifan Fei,Ting Ye,Qiusha Li,Xiaoshun Zhou,Ning Gan,Yong Shao
Efficient structuring of DNA by small molecules is very crucial in developing DNA-based novel switches with an ideal performance. In this work, we found that inverting only the polarity of the 3′ terminal guanine of the thrombin-binding aptamer (3iTBA) totally eradicates the original TBA G-quadruplex (G4) structure in K+. The unstructured 3iTBA can be further refolded upon specifically interacting with small molecules of natural isoquinoline alkaloids (IAs) due to their fruitful binding patterns with variant nucleic acid structures. We identified that 3iTBA can serve as a topology selector for planar IAs. Nitidine (NIT), owing to the planar aromatic ring and coplanar substituents, is the most efficient to restructure the 3iTBA random coil toward the anti-parallel G4 conformation. However, common metal ions can't realize this structuring. The topology selector competency of 3iTBA toward IAs’ planarity can be visualized using gold nanoparticles (AuNPs) as the chromogenic readout. Our work expands the G4 repertoire by exploring the polarity inversion regulation and provides a new approach to switch nucleic acid structures toward a small molecule structure-sensitive sensor.
研究领域:
生物化学
·
分子生物学
·
纳米技术
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 7
·1-8
·
IF 6.232
Liu Yaqing,Lin Xiaodong,Ji Xiangyi,Hao Zhe,Tao Zhanhui
A novel fluorescence strategy based on the outstanding catalytic capability of CuS nanoparticles (CuS NPs ) has been developed for highly sensitive and specific determination of o,o-dimethyl-o-2,2-dichlorovinyl phosphate (DDVP) under enzyme-free and hydrogen peroxide (H 2 O 2 )-free conditions. In the presence of DDVP, CuS NPs can catalyze non-fluorescence substratum of Amplex red (AR) into resorufin, which exhibits fluorescence emission at 584 nm under excitation at 540 nm. The sensing system exhibits outstanding specificity and only responds to DDVP and no other organophosphorus pesticides (OPs). A wide linear range is obtained from 0.0001 to 0.1 μg/mL, and the limit of detection (LOD) is 0.1 ng/mL. Furthermore, paper-based test strips have been constructed for visual detection of DDVP under ultraviolet light irradiation. By integrating a smartphone installed with Color Picker APP, point-of-care detection with quantitative determination is realized, demonstrating substantial potential applications of the as-developed assay for in situ detection.
Graphical abstract
研究领域:
分析化学
·
环境科学
·
生物技术
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 114
·93850-93857
·
IF 3.84
Wei Li,Guo-Sheng Ding,An-Na Tang
The immobilization of popular chiral selectors on the surface of nanomaterials to prepare new chiral adsorbents for preparative chiral separation is a research hotspot in separation science nowadays. In this study, bovine serum albumin (BSA) modified silica nanoparticles were prepared by using polydopamine (PDA) as a versatile multifunctional secondary reaction platform. The preparation method was facile, cost-effective and environmentally friendly. The new chiral adsorbents were then investigated for the separation of representative chiral drug enantiomers. For propranolol and tryptophan, the multi-step adsorption enhanced the chiral performance to a great degree. On increasing the starting percent enantiomeric excess (%, e.e.) of the enantiomeric mixtures, the %, e.e. value of the resulting solution increased to almost 100% under the same operating conditions. For simplicity and rapidness, the results of adsorption were measured by capillary electrophoresis (CE) using carboxymethyl-β-cyclodextrin (CM-β-CD) or α-cyclodextrin (α-CD) as additives into the background electrolyte solution. The experimental results also showed that the thus-prepared nanomaterials could be readily recycled at least three times, demonstrating their great stability and possibility in practical use.
研究领域:
分离科学
·
纳米技术
·
药物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）