JOURNAL OF SEPARATION SCIENCE
·
2018
·Vol. 42
·期 5
·1037-1044
·
IF 2.415
Wenjuan Zhang,Liyan Jiang,Li Fu,Qiong Jia
Efficient separation and enrichment of low-abundance glycopeptides from complex biological samples is the key to the discovery of disease biomarkers. In this work, a new material was prepared by coating copper tetra(N-carbonylacrylic) aminephthalocyanine and iminodiacetic acid onto poly(glycidyl methacrylate-pentaerythritol triacrylate) monolith. The monolith was applied to polymer monolithic microextraction for specific capture of glycopeptides coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The developed monolith exhibited satisfactory efficiency for glycopeptide enrichment with high selectivity and detection sensitivity. When the tryptic digest of immunoglobulin G was used as the sample, total 24 glycopeptides were identified and the detection limit was determined as 5 fmol. When the approach was applied to the analysis of glycopeptides in the mixture of bovine serum albumin and immunoglobulin G (100:1, m / m ) digests, 16 glycopeptides could still be observed. Moreover, the monolith was successfully applied to the selective enrichment of glycopeptides from human serum digests, exhibiting great practicability in identifying low-abundance glycopeptides in complex biological samples.
研究领域:
分析化学
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蛋白质组学
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生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Huijia Song,Haipin Zhang,Yulian He,Ruixia Gao,Yue Wang,Wenting Wang,Lisa D. Pfefferle,Xiaoshuang Tang,Yuhai Tang
Rutin (RT), a widely distributed natural flavonoid compound, has been generally utilized as an important active ingredient owing to its considerable biomedical and economic value. Inspired by the structure features of densely-packed bayberry and well-orientated honeycomb, a novel type of magnetic molecularly imprinted polymers (HB-TI-MMIPs) with abundant high-affinity and uniformly-distributed binding sites was rationally constructed for the selective enrichment of RT from Sophora japonica. The polymerization conditions, physicochemical properties, and adsorption performance of the imprinted nanomaterials were systematically investigated. The optimized HB-TI-MMIPs display a high adsorption capacity, fast adsorption rate, and satisfactory selectivity towards RT. Meanwhile, the proposed analytical methodology using HPLC, with HB-TI-MMIPs as adsorbents, successfully applied to enrich and detect RT from Sophora japonica with high recoveries (87.2–94.6%) and good RSDs (lower than 4.3%). Therefore, the fabricated HB-TI-MMIPs with a fast magnetic responsivity and desirable adsorption performance would be attractive in plant active ingredients extraction fields.
研究领域:
植物化学
·
分析化学
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药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Jie-Ping Fan,Ping Gan,Feng-Hui Tao,Hui-Ping Chen,Hai-Long Peng,Yu-De Liu,Chun-Fang Xie
Oleanolic acid possesses a wide variety of pharmacological activities and spreads in various plants, but always co-exist with its isomers ursolic acid. Separating oleanolic acid with high efficacy from ursolic acid contaminated plant extracts is quite important. This study prepared a molecularly imprinted poly(OA[AMI] supramolecular hydrogel) (MIPSHG@OA) for selectively enriching oleanolic acid, by using the 1-allyl-3-methylimidazolium oleanolic salt (OA[AMI]) as hydrogelator, template, functional monomer and basement. The preparation conditions were optimized as 12.5 mg OA[AMI], 40 mg MBA, 4 ml solvent, 10 mg APS, and 30 μl TEMED. The characterization by FT-IR spectra, TG analysis, SEM image and equilibrium swelling ratio showed a sheet and porous structure and a large equilibrium swelling ratio of the obtained MIPSHG@OA. The adsorption performance showed the obtained MIPSHG@OA was homogeneous on the surface and uniform on the adsorption sites, the adsorption of oleanolic acid on the polymers was chemisorption and monolayer, the adsorbed oleanolic acid molecules did not interact with each other, the MIPSHG@OA had high competitive recognition performance and imprinting efficiency on oleanolic acid, and had good recyclability. In the solid phase extraction application, the results demonstrated that the synthesized MIPSHG@OA could selectively enrich oleanolic acid.
研究领域:
分析化学
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药学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Kun Ge,Yi Peng,Zicheng Lu,Yuling Hu,Gongke Li
In this work, we introduced an aptamer modified Au nanoparticles doped covalent organic frameworks composite (IBAs-AuNPs/COF) to improve the property of selective enrichment of insulin from serum samples. The Au nanoparticles were immobilized on imine-based COF by in-situ reduction reaction via mussel inspired polydopamine coating, and then sulfhydryl-containing aptamers were bonded to the surface of AuNPs through an Au-S linkage. Due to the excellent adsorption property of COF and specific recognition between insulin and IBAs, the IBAs-AuNPs/COF composites show selective and satisfactory extraction property to insulin in serum samples. Excellent specifity was obtained for insulin in the presence of 50-fold interfering substances including human immunoglobulin, lysozyme and biotin . The concentrations of insulin in the range of 1.0 to 50.0 μg L −1 show good linear relationship ( R 2 = 0.9917) with limit of detection and limit of quantitation of 0.28 μg L −1 and 0.93 μg L −1 , respectively. Then, the IBAs-AuNPs/COF composites were applied to enrich insulin in serum samples followed by analysis with matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS). After the recovery experiment, the developed method shows good recoveries in range of 91.6%–112.4% with low RSD value (2.4%–9.4%, n = 3) for diabetic and healthy serum samples. The developed IBAs-AuNPs/COF composites propose a new perspective for selective and efficient enrichment of biomarkers in serum samples by functionalized COF.
研究领域:
分析化学
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生物医学工程
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纳米技术
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS CHEMISTRY
·
2012
·Vol. 22
·期 45
·23981-23988
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IF 5.968
Wan-Fu Ma,Lu-Lu Li,Ying Zhang,Qiao An,Li-Jun You,Ju-Mei Li,Yu-Ting Zhang,Shuai Xu,Meng Yu,Jia Guo,Hao-Jie Lu,Chang-Chun Wang
Selective enrichment of glycopeptides from complicated biological samples is essential for MS-based glycoproteomics, but still remains a great challenge. In this study, we report an unprecedented ligand-free strategy for the selective enrichment of glycopeptides by simply utilizing the multivalent interaction between glycopeptides and silver nanoparticles (Ag-NPs) coated magnetic nanoarchitectures. The composite microspheres were deliberately designed to be constructed with a high-magnetic-response magnetic colloid nanocrystal cluster (MCNC) core, a poly(methacrylic acid) (PMAA) interim layer and a Ag-NPs functional shell with high coverage. Taking advantage of the reversible interaction of glycans with Ag-NPs and the high magnetic susceptibility of the magnetite core, the MCNC@PMAA@Ag-NPs microspheres possess remarkable selectivity for glycopeptides even at a low molar ratio of glycopeptides/non-glycopeptides (1 : 100) with a very rapid enrichment speed (only 1 min needed) and a simple operation procedure using magnetic separation. Applying this approach, we identified 127 unique glycopeptides mapped to 51 different glycoproteins from only 1 μL rat serum samples. These results clearly demonstrated that the MCNC@PMAA@Ag-NPs have great potential for purifying and identifying the low-abundant glycopeptides in complex biological samples.
研究领域:
蛋白质组学
·
生物化学
·
纳米技术
✓ 已验证
DOI
高影响因子
Plants-Basel
·
2024
·Vol. 14
·期 1
·56
·
IF 4
Yanhui Wang,Linlin Yan,Guangyao Zheng
Rosmarinic acid (RA) is a natural active compound widely found in many plants belonging to the family ofLamiaceae,Boraginaceae, and so on, which has various important bioactivities, including being anti-oxidative, anti-inflammatory, antiviral, etc. Herein, novel hydrophilic magnetic molecularly imprinted polymers (HMMIPs) with a regular core-shell structure were successfully developed using RA as a template molecule, acrylamide (AM) as a functional monomer, N-N ’methylenebisacrylamide (MBA) as a cross-linking agent, and water as the porogen. After a series of characterization and adsorption performance analyses, it was found that HMMIPs are hydrophilic with an adsorption capacity of 8.012 ± 0.54 mg/g, an imprinting factor of 3.64, and a selectivity coefficient of 2.63~2.91. Furthermore, the HMMIPs can be rapidly separated from other components under the influence of external magnetic fields. The HMMIPs were employed for the determination of RA present in thePerilla frutescensandRosmarinus officinalisaqueous extract with recoveries of 88.2~107.3%. These results indicated that HMMIPs of RA have the benefits of straightforward operation, rapid adsorption, and high selectivity, rendering it an appropriate way for the expedient and selective isolation of RA in an intricate matrix.
研究领域:
植物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2014
·
IF 5.9
Xiao-Mei He,Gang-Tian Zhu,Yuan-Yuan Zhu,Xi Chen,Zheng Zhang,Shao-Ting Wang,Bi-Feng Yuan,Yu-Qi Feng
Sulfhydryl cotton fiber (SCF) has been widely used as adsorbent for a variety of metal ions since 1971. Thanks to the abundant thiols on SCF, in this study, we reported a universal method for the facile preparation of SCF-based materials using “thiol–ene” click chemistry for the first time. With the proposed method, two types of SCF-based materials, phenylboronic acid grafted sulfhydryl cotton fiber (SCF-PBA) and zirconium phosphonate-modified sulfhydryl cotton fiber (SCF-pVPA-Zr4+), were successfully prepared. The grafted functional groups onto the thiol group of SCF were demonstrated by X-ray photoelectron spectroscopy (XPS) and energy dispersive X-ray spectroscopy (EDX). The prepared fibrous materials exhibited excellent fiber strength, good stability in aqueous or nonaqueous solutions, and great biocompatibility. Moreover, we developed filter-free in-pipet-tip SPE using these SCF-based materials as adsorbent for the enrichment of ribonucleosides, glycopeptides and phosphopeptides. Our results showed that SCF-PBA adsorbent can selectively capture ribonucleosides and glycopeptides from complex biological samples. And SCF-pVPA-Zr4+ adsorbent exhibited high selectivity and capacity in the enrichment of phosphopeptides from the digestion mixture of β-casein and bovine serum albumin (BSA), as well as human serum and nonfat milk digest. Generally, the preparation strategy can be a universal method for the synthesis of other functionalized cotton-based adsorbents with special requirement in microscale biological analysis.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of the Taiwan Institute of Chemical Engineers
·
2018
·
IF 3.849
Juan Zhao,Yilin Wu,Chen Wang,Hai Huang,Jian Lu,Xiuling Wu,Jiuyun Cui,Chunxiang Li,Yongsheng Yan,Hongjun Dong
A facile, yet efficient method was proposed here to prepare the novel molecularly imprinted nanocomposite membranes (MICMs) with high adsorption capacity and rapid selective separation aiming at targeting norfloxacin. The MICMs were prepared by vacuum filtering directly pre-synthesized norfloxacin-imprinted nanocomposites to the regenerated cellulose membranes, which utilized adequately the dual-function effect of dopamine as crosslinking agent and functional monomer. Moreover, the best adsorption capacity and separation factor of MICMs toward target can reach up to 25.35 mg/g and 4.43, respectively, which were far superior to that of non-imprinted nanocomposite membranes (NICMs) (6.38 mg/g and 1.0, respectively). Meanwhile, the perm-selectivity (the permeability factor β values were also more than 15.2). And this work provided an exemplary guidance for the treatment of fluoroquinolones antibiotics pollutants in water environment.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION SCIENCE AND TECHNOLOGY
·
2015
·
IF 1.171
Cuimei Lu,Hui Li,Miaomiao Xu,Susu Wang,Gui Li,Wei Zhong,Siyin Qin
A novel surface imprinting technique combined with in-situ polymerization was developed to prepare nicotine-imprinted monolith (MIPM) in a stainless column by grafting molecularly imprinted polymers (MIPs) onto the internal hole surface of macroporous silica. Binding capacity of this MIPM was evaluated by frontal chromatographic technique and the MIPM solid phase extraction researched. Then, the applicability for this MIPM to selectively gather nicotine in environmental water sample was evaluated. Results indicated that the pH value of mobile phase greatly influenced binding capacity of the MIPM. When the mobile phase was at pH 5.0, adsorption capacity was the highest, with a value of 6.4 mg/L. Furthermore, when a pH 9.0 phosphate buffer was used as elution solvent during solid phase extraction, the target compound could be selectively desorbed from the MIPM, with a recovery of 99.6%. In addition, no great loss in recovery emerged when this MIP monolith was repeatedly used for solid phase extraction of nicotine in environmental water sample, revealing a good repeatability.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Yi Hao,Jian Shang,Shuyan Liu,Xuemeng Tian,Xin Yao,Fengjun Liu,Ruizhen Zhu,Yue Wang,Ruixia Gao,Sicen Wang
The widespread presence of antibiotic residues in animal-derived foods poses serious risks to public health and the environment, emphasizing the need for urgent action. This study presented a novel lightweight, popcorn-like magnetic molecularly imprinted polymer (LPR-MMIPs) with protein-resistant properties, for the selective enrichment of tetracycline (TC) antibiotics in milk. During the preparation of LPR-MMIPs, bovine serum albumin (BSA) was used as the functional monomer, and tris(2-carboxyethyl)phosphine (TCEP) served as the reducing agent. Using a “one-stone-two-birds” strategy, TCEP not only converted the α -helical structure of BSA to a β -folded conformation for imprinting on the Fe 3 O 4 carrier, but also etched the Fe 3 O 4 into a lightweight, popcorn-like structure under acidic conditions. The BSA imprinting layer excludes proteins through electrostatic repulsion, and the reduced amount of carrier material significantly enhances the adsorption efficiency ( Q = 12.7 mg g −1 ), selectivity ( IF = 3.02, SC > 1.53), and reusability. Meanwhile, LPR-MMIPs, as solid-phase extraction adsorbents, have been successfully applied to the specific adsorption and separation of TC in real milk samples. The established method exhibits good accuracy, precision, and sensitivity, as evidenced by the low LOD (1.80 ng mL −1 ) and LOQ (5.60 ng mL −1 ), low RSDs (≤5.4 %), and high recovery rates (≥94.5 %). Besides, the method demonstrates excellent practical applicability for milk, offering a novel strategy for the selective enrichment of trace antibiotics in milk.
研究领域:
食品科学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2011
·
IF 4.311
Ya Yuan,Yuzhi Wang,Meidong Huang,Ran Xu,Huan Zeng,Chan Nie,Jinhuan Kong
In the present work, microwave heating initiated precipitation polymerization was developed to prepare podophyllotoxin (PPT) molecularly imprinted polymers (MIPs), resulting in much shorter polymerization time and better particle morphology. Prior to the polymerization, ultraviolet and FTIR spectroscopy were used to study the interactions between PPT and the functional monomers . The synthesized parameters were respectively optimized and the optimal conditions for the efficient adsorption property were template: PPT, 1 mmol; functional monomer: acrylamide , 6 mmol; bi-crosslinker: ethylene glycol dimethacrylate , 20 mmol and divinylbenzene, 20 mmol; porogen : acetonitrile , 40 mL; initiator: azobisisobutyronitrile, 0.01 mol L −1 ; polymerization temperature: 60 °C. FTIR spectroscopy, SEM and thermal analysis were used to characterize the MIPs. The results of the equilibrium rebinding experiments and the competitive adsorption experiments showed that these imprinted polymers exhibited good adsorption ability for the PPT. Scatchard analysis illustrated that two and one types of binding sites were generated in the MIPs and non-imprinted polymers (NIPs), respectively. Using the prepared MIPs as the solid phase extraction (SPE) sorbent , PPT was extracted selectively and efficiently from Dysosma versipellis , Sinopodophyllum hexandrum and Diphylleia sinensis . The regression equation was y = 5.873 × 10 6 x + 17075.659 with the correlation coefficient of 0.9994 in the concentration range of 0.005–0.4 mg mL −1 . After washing and eluting the SPE column with methanol and MeOH/acetic acid solution (v/v, 9:1), the limits of detection were 0.12–0.18 μg mL −1 and their recoveries were in the range of 89.5–91.1% with all RSDs lower than 3.7.
研究领域:
药学
·
分析化学
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Wei Luo,Jinteng Chen,Huiting Lin,Xiaoxia Ye
60 Co is widely utilized in industries and can emit high gamma energy. The removal radioactive 60 Co in trace concentration from wastewater is important for human health and environment protection. Here, we designed a biomass base membrane with phenol hydroxyl-amino group for ultraselective enriching of trace concentration of Co 2+ from radioactive wastewater. This novel membrane adsorbent (PAM–PBWT), that is, the immobilized black wattle tannin (BWT) modified with polyethylenimine onto the polyamide microporous membrane (PAM), utilizes phenolic hydroxyl and amino group can form a coordinated coordination with Co 2+ , exhibiting high selectivity adsorption to Co 2+ even in the presence of other ions. A maximal adsorption capacity (0.39 mmol g −1 ) was reached at pH 8.0 and 323 K. The continuous adsorption experiments showed that the PAM–PBWT can enrich the trace concentration of Co 2+ (60 ng L −1 ) from radioactive wastewater in the presence of other coexisting ions (K + , Ca 2+ , Na + , Mg 2+ , Zn 2+ , Mn 2+ , Fe 3+ ). Moreover, the processing capacity of three-layer PAM–PBWT for simulated 60 Co-containing wastewater can reach 80 L m −2 . Notably, PAM–PBWT can be easily regenerated with 0.1 M HNO 3 without the loss of adsorption capacity. Our work might introduce a new strategy for realizing selectivity adsorption of trace concentration irradiation contaminants from radioactive wastewater.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）