Materials Science & Engineering C-Materials for Biological Applications
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2018
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IF 5.08
Huikang Yang,Nianhua Wang,Lei Mo,Mei Wu,Ruimeng Yang,Xiangdong Xu,Yugang Huang,Jiantao Lin,Li-Ming Zhang,Xinqing Jiang
Tumor-targeted multifunctional nanocarriers play an important role in tumor diagnosis and treatment. Herein, disulfide bonds linked amphiphilic hyaluronan-SS-poly(ε-caprolactone) diblock copolymers (HA-SS-PCL) were synthesized and studied as theranostic nanocarriers for tumor diagnosis and treatment. The chemical structure of HA-SS-PCL was confirmed by Fourier transform infrared spectroscopy (FTIR) and proton nuclear magnetic resonance ( 1 H NMR). The self-assembling behavior of the HA-SS-PCL into GSH-responsive micelles and their degradation were characterized by fluorescence spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy (TEM). Theranostic nanocarriers encapsulating doxorubicin (DOX) and superparamagnetic iron oxide (SPIO) were formed via a dialysis. In vitro drug release results suggested that the HA-SS-PCL micelles possessed reductant-triggered doxorubicin release ability, which was confirmed by 100% of DOX release from HA-SS-PCL micelles within 12 h under 10 mM of glutathione (GSH), whereas about 40% of DOX was released under non-reductive condition within 24 h. Both flow cytometry and confocal laser scanning microscopy (CLSM) analysis revealed that the HA-SS-PCL micelles loaded with DOX were internalized in HepG2 cell via a receptor mediated mechanism between hyaluronan and the CD44 receptor. Furthermore, the MTT assay and cell apoptosis analysis revealed that the DOX-loaded HA-SS-PCL micelles exhibited pronounced antitumor ability towards HepG2 cells compared with that of the reduction-insensitive HA-PCL micelles at the same DOX dosage. The r 2 relaxivity value of the DOX/SPIO loaded HA-SS-PCL micelles was up to 221.2 mM −1 s −1 (Fe). Thus, the obtained HA-SS-PCL block copolymers demonstrate promising potential as tumor targeting theranostic nanocarriers in the field of tumor diagnosis and treatment.
研究领域:
生物材料
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肿瘤学
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药剂学
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2020
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IF 4.845
Kaipei Luo,Shaoping Yin,Ruixia Zhang,Hua Yu,Guangji Wang,Juan Li
The efficient delivery of chemotherapeutic drugs to the tumor tissues unavoidably encounters numerous obstacles, such as poor tumor targeting capability, slow intracellular drug release and massive accumulation in the liver. In this study, by self-assembling methoxy poly (ethylene glycol)-poly (lactide) block copolymer (mPEG-PLA) and hyaluronic acid-paclitaxel conjugate (HA-PTX), the composite nanoparticles (mPPHP NPs) were fabricated for efficient therapy of cancer. mPPHP NPs formed self-assembled nanoparticles (116 nm in diameter) with a narrow size distribution; and showed a rapid release of PTX in the presence of hyaluronidase and esterase . mPPHP NPs exhibited enhanced internalization by cells via CD44 receptors and selected cytotoxicity against A549 cells in vitro . More importantly, compared with other PTX formulations, mPPHP NPs were demonstrated to present the reduced liver accumulation, excellent tumor-targeting ability and superior antitumor efficacy in vivo , with a TIR of 75.9%. The multifunctional composite nanoparticles could be developed as a promising nano-carrier for improved therapeutic efficacy.
研究领域:
肿瘤学
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纳米医学
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药剂学
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分子靶向治疗
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药物递送系统
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转化医学
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ACS Biomaterials Science & Engineering
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2025
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IF 5.5
Bo Li,Chenchen Li,Ziyi Yan,Ping Ji,Xiaoling Yang,Hongsi Yu,Congyan Xia,Fengyi Xie,Meilin Zhao,Bo Hu
Promoting the regeneration of bacteria-infected damaged tissues by developing multifunctional tough hydrogels with self-healing, adhesive, remodeling, and antimicrobial properties for use as wound dressings remains a great challenge. Inspired by the excellent adhesion ability of natural mussels, hyaluronic acid (HA) was first oxidized to aldehyde hyaluronic acid (AHA), and then grafted with dopamine (DA) to produce a structure with many catechol groups from which dopamine-grafted hyaluronic acid (DAHA) was prepared and combined with acrylamide (AM), poly(acrylic acid) (PAA), and N,N′-methylenebis(acrylamide) (BIS) to prepare a multifunctional hydrogel (PAD hydrogel). The results indicated that the PAD3 hydrogel with a DAHA content of 3 mg/mL and a BIS monomer content of 0.5 mol %, had high mechanical strength (over 10 MPa), which was 27 times greater than that of pure polyacrylamide (PAM) hydrogels. Furthermore, the PAD hydrogel demonstrated strong antibacterial activity against both Gram-positive and Gram-negative bacteria, and the ability of it to repair infected skin was further investigated in a rat model of Staphylococcus aureus infection. The PAD series hydrogels showed considerable antimicrobial properties and promoted the regeneration of damaged tissues in vivo, suggesting that it can be used as a multifunctional dressing and holds great promise in healing bacteria-infected skin wounds.
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Advanced Healthcare Materials
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2025
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IF 9.6
Yunfeng Tang,Xiangtian Deng,Min Yi,Dong Wang,Cheng Zheng,Shichang Zhao,Renliang Zhao
Melanoma remains one of the most aggressive skin cancers with high recurrence rates following surgical resection. To overcome the challenges of residual tumor cells and postoperative immune resistance, an injectable hydrogel is developed that co-delivers propranolol (Pro) and imiquimod (IMQ). The rationale is to achieve a synergistic therapeutic effect by enhancing immunogenic cell death (ICD) and strengthening antitumor immunity. The hydrogel is synthesized via boronate ester crosslinking between HA-EGCG and CMCS-PBA and demonstrates excellent injectability, self-healing, biodegradability, and tissue adhesion. In vitro, Pro potentiated IMQ-induced reactive oxygen species (ROS) production, enhancing ICD markers such as calreticulin exposure (CRT), high mobility group box 1 (HMGB1), and adenosine triphosphate (ATP) release, thereby promoting dendritic cell maturation and T cell activation. In vivo, Pro/IMQ@ Hydrogel (PIH) effectively inhibited tumor recurrence in an incomplete melanoma resection model, significantly reducing tumor volume and inducing apoptosis without systemic toxicity. Immunohistochemical analyses revealed increased CD8⁺ and CD4⁺ T cell infiltration, elevated inflammatory cytokine levels, and enhanced memory T cell responses. This work highlights the potential of PIH as a potent localized chemo-immunotherapy platform for melanoma by converting “cold” tumors into “hot” immunogenic lesions.
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ACS Applied Materials & Interfaces
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2025
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IF 8.2
Dan Zu,Jun Yao,Xiao Li,Yixing Huang,Can Hu,Mingcong Deng,Qimei Bao,Yin Shi,Zu Ye,Xiangdong Cheng
Gastric cancer (GC) is one of the most common malignant tumors in the world, which is resistant to conventional chemotherapy drugs. Although polyphyllin B (PB) exhibits antitumor activity against GC, its clinical application is limited by systemic toxicity and poor target specificity. Here, we developed a dual-targeting nanoparticle (Ma/HA@NP) coated with a gastric cancer cell membrane (Ma) and hyaluronic acid (HA) to enhance biocompatibility, immune evasion, and homologous targeting. Ma/HA@NPs preserve the immune evasion and homologous targeting properties of both components. Upon endocytosis, Ma/HA@NPs react with cysteine to release PB, deplete glutathione (GSH), and reduce the enzymatic activity of glutathione peroxidase 4 (GPX4), leading to ferroptosis activation. Compared to single PB, Ma/HA@NPs not only enhance PB release but also trigger immunogenic cell death, promoting dendritic cell maturation and cytotoxic T lymphocyte activation. Moreover, interferon-γ release further suppresses cysteine uptake, synergistically amplifying ferroptosis. In vivo, intravenous administration of Ma/HA@NPs-PB in an orthotopic GC model achieved an 85.4% tumor inhibition rate with reduced systemic toxicity compared to free PB. Together, this dual-targeting platform enhances PB delivery and synergizes ferroptosis with immunogenic cell death (ICD) activation, offering a promising strategy for the treatment of gastric cancer.
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ANALYTICAL CHEMISTRY
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2025
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IF 6.7
Muhammad Saqib,Wen-Xin Chen,Yun Lei,Ai-Lin Liu
Chemiluminescence (CL) assays are often constrained by the limited availability of coreactants that combine strong signal amplification with biocompatibility. Herein, we report for the first time that sericin, a natural protein derived from silk, serves as a highly efficient coreactant in the lucigenin CL system under alkaline conditions. Impressively, this new lucigenin–sericin CL system generates an unprecedented ∼43-fold higher emission intensity compared to the traditional lucigenin–hydrogen peroxide system. Moreover, the mechanistic studies reveal that sericin, with its electron-rich functional groups, significantly promotes the generation of superoxide radicals (O2•–). This, in turn, accelerates the lucigenin oxidation pathway, leading to significant enhancement in CL signal. Leveraging this enhanced system, we developed a dual-function CL platform for the ultrasensitive detection of sericin (limit of detection, LOD = 0.12 μM) and superoxide dismutase (SOD, LOD = 0.28 ng/mL), exhibiting an extended linear range spanning from 0.25 to 50 μM and 0.5 to 60 ng/mL, respectively, the latter based on CL quenching phenomena. This method offers compelling superiority in terms of simplicity, rapid analysis, enhanced sensitivity, higher selectivity, and cost-effectiveness. Moreover, this method successfully achieved the detection of sericin in cosmetics and the SOD in human serum samples. Importantly, this work represents the first chemiluminescent method for sericin detection. In addition, the system exhibited high sustainability and practical utility, as confirmed by AGREE (greenness) and BAGI (blueness) assessment tools. Furthermore, it introduces a new class of protein-based coreactants for CL systems, expanding the frontier of (bio)analytical detection.
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Journal of Materials Chemistry B
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2025
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IF 5.7
Kunrui Liu,Hengjie Zhang,Bao Xia,Wenjing Liu,Junmei Song,Jianhua Zhang,Zhipeng Gu,Zhan Li,Xianchun Chen
Severe hemorrhage and wound inflammation are major risk factors contributing to high mortality after tissue trauma. Therefore, there is an urgent need to develop emergency materials that can rapidly and effectively close wounds while simultaneously controlling bleeding and infection. Although current clinical bioadhesives can fill surgical voids and support tissue repair, they generally lack sufficient adhesive strength and anti-inflammatory properties, which limit their effectiveness in inflammatory wound environments. In this study, a kind of bioadhesive hydrogel was developed using a simple heating and mixing strategy with natural building blocks, including polysaccharides, lipoic acid, and natural polyphenol extracts. Through multiple non-covalent interactions (such as hydrogen bonding and electrostatic interactions), the resulting bioadhesive hydrogels exhibited excellent tissue adhesion and hemostatic properties. Moreover, these hydrogels also demonstrated outstanding anti-inflammatory effects, biocompatibility, and favorable biodegradability, effectively promoting both linear and burn wound healing. This work presents a novel strategy for achieving strong bioadhesion using natural molecules and provides a promising approach for the development of multifunctional wound dressings designed to support tissue regeneration.
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Hongyan Lu,Ying Cheng,Xiangling Pu,Xingtao Zhao,Ruolan Li,Ruiqi Sun,Xiaoman Xie,Mingyue Wu,Shuo Zhang,Qiong Pan,Jin Chai
Simultaneously alleviating oxidative stress and remodeling inflammatory microenvironment holds great promise for treating inflammatory bowel disease (IBD). Nevertheless, the efficacy of conventional therapies remains limited by poor targeting, insufficient modulation of the inflammatory microenvironment and serious adverse effects. Here, inspired by the intrinsic biological properties of metal-curcumin (Cur) complexes, a nanozyme-mediated ion-exchange strategy was proposed to enhance the targeted therapeutic effect of Cur for IBD. Specifically, copper-doped mesoporous polydopamine nanoparticles (Cu-mPDA) with dual enzyme-like activities were served as drug carriers to encapsulate water-soluble zinc-curcumin (Zn-Cur) and further modified with hyaluronic acid (HA), yielding Zn-Cur/Cu-mPDA@HA. Encouragingly, Zn-Cur/Cu-mPDA@HA could degrade in inflammatory microenvironment, releasing Zn-Cur and Cu 2+ , which subsequently in situ generate Cu-Cur with high antioxidant activity by ion-exchange reaction. Notably, Zn-Cur/Cu-mPDA@HA not only exhibited favorable targeting specificity toward M1 macrophages in inflamed colon tissues, but also displayed enhanced abilities to scavenge ROS, inhibit pro-inflammatory factors secretion and promote macrophages repolarization from the pro-inflammatory M1 state to the anti-inflammatory M2 subtype. Mechanistically, Zn-Cur/Cu-mPDA@HA suppressed the TLR4/MyD88/NF-κB pathway, breaking the inflammation-free radical vicious cycle, thereby attenuating chronic inflammation and fostering a favorable microenvironment for intestinal barrier restoration. Such a nanozyme-mediated ion-exchange and in situ drug generation strategy offers a new insight for designing nanomedicines for the treatment of IBD and other inflammatory diseases.
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JOURNAL OF CONTROLLED RELEASE
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2025
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IF 11.5
Zhiwei Chen,Xinyu Di,Heyan Chen,Shengnan Song,Siyi Zhu,Leer Cai,Yuqi Han,Yongshuai Kang,Dingchao Shen,Huirong Huang,Aimin Cai,Hailun Zheng,Qing Yao,Maoping Chu,Ruijie Chen,Longfa Kou
Coronary artery lesions are a major complication of Kawasaki disease (KD), where the local microenvironment is marked by excessive oxidative stress and persistent inflammation, presenting significant challenges for effective treatment. M1-polarized macrophages dominate these lesions, releasing pro-inflammatory mediators that aggravate vascular injury. Regulating macrophage polarization from the M1 to M2 phenotype thus offers a promising therapeutic strategy. Here, we developed a reactive oxygen species (ROS)-responsive nanoplatform, HA-Sim@BRPL, to deliver simvastatin (Sim) specifically to inflamed coronary tissues. The nanocarrier is constructed from poly- l -lysine conjugated with bilirubin (BR), which self-assembles and enables ROS-triggered Sim release through BR's oxidative conversion to hydrophilic biliverdin. Surface decoration with hyaluronic acid (HA) facilitates selective uptake by CD44-expressing macrophages rather than cardiomyocytes. HA-Sim@BRPL effectively reduces intracellular ROS, promotes M2 macrophage polarization, and enhances endothelial cell survival via intercellular signaling. In a KD mouse model, HA-Sim@BRPL significantly attenuated coronary artery lesions and improved cardiac function. These results highlight a rationally designed nanomedicine that integrates oxidative stress responsiveness and inflammatory cell targeting, providing a promising therapeutic approach for coronary complications in KD.
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Frontiers in Bioengineering and Biotechnology
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2026
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IF 5.8
Xianglong Yi,Yue Song,Liqun Chen,Riye Su,Bo Liu,Xiaohui Tang,Qing Wei,Yingbo Wang,Wenbo Cheng
Corneal stromal lenticules obtained through small incision lenticule extraction (SMILE) procedures offer a valuable graft material for therapeutic applications. Current clinical utilization faces challenges due to intrinsic thinness (<140 μm) and restricted dimensions (generally around 6.6 mm). This study introduced a novel approach to enable the construction of customizable corneal grafts by stacking lenticules, achieving specific thickness and diameter for diverse corneal defects, using photo-crosslinked dual-network hydrogels based on methacrylated gelatin (GelMA). In vitro characterization confirmed the hydrogel’s suitable morphological architecture, optical clarity, and excellent biocompatibility, establishing it as an optimal biological adhesive for sutureless graft implantation. This multi-lenticule encapsulation strategy using the hydrogels successfully reconstructed experimental rabbit corneal defects (7.0-mm diameter) in vivo . Over a 5-week postoperative period, the hydrogel demonstrated controlled biodegradation while maintaining structural integrity and optical functionality throughout tissue remodeling. It effectively adhered to the surrounding stromal tissues and supported epithelial regeneration over the transplanted grafts. The study demonstrates sutureless-free corneal stromal lenticule implantation, enabled by the GelMA-based photocrosslinked dual-network hydrogel, addressed the limitations of individual SMILE lenticules. The GelMA-based photocrosslinked dual-network hydrogel serves as both a biocompatible adhesive for multi-lenticule implantation and an optimal functional material for reconstructing corneal defects.
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BIOMACROMOLECULES
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2026
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IF 5.9
Jing Guo,Kun Luo,Xin-Yu Dai,Yao Li,Teng Fu,Lei-Xiao Yu,Ke-Ke Yang
Osteoarthritis (OA) is the most common degenerative joint disease, which is largely driven by the deterioration of cartilage lubrication. However, current lubricants, such as hyaluronic acid (HA), suffer from poor mechanical resilience and rapid enzymatic degradation. Herein, we develop a structurally engineered lubricant, HPG-g-pSBMA (HgS), constructed by grafting zwitterionic poly(sulfobetaine methacrylate) (pSBMA) chains onto a globular hyperbranched polyglycerol (HPG) core. The hyperbranched topology minimizes molecular entanglement and shear thinning, while the dense zwitterionic shell forms a robust hydration layer that resists compression and frictional wear. HgS exhibits superior lubrication efficacy at a low concentration (1 mg/mL) with enhanced injectability, significantly outperforming 10 mg/mL HA. Molecular simulations reveal strong hydration shell formation and hydration-mediated disruption of solute–solute interactions. HgS demonstrates excellent biocompatibility and enzymatic stability, prolonged intra-articular retention, and effective inhibition of OA progression in vivo. This work highlights a topology-guided design strategy for durable and efficient joint lubricants for OA treatment.
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ACS Applied Materials & Interfaces
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2026
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IF 7.8
Xinyi Guo,Mengmeng Wang,Danyang Qu,Hao Cui,Jiajing Gong,Qinglin Shen,Yang Han,Xingqi Pan,Zhenbao Liu,Meilin Shi
Sonodynamic therapy (SDT) offers a noninvasive strategy for tumor ablation through ultrasound-activated sonosensitizers that generate reactive oxygen species (ROS); however, its therapeutic efficacy is often limited by tumor hypoxia. Herein, we report the rational design of a multifunctional theranostic nanoplatform, HA-MnO2-TCPP nanoflowers, that integrates active tumor targeting, dual ROS amplification, and magnetic resonance imaging (MRI) guidance for synergistic sonodynamic and chemodynamic therapy. The MnO2 nanoflowers were loaded with the sonosensitizer 4-carboxyphenyl porphyrin (TCPP) and surface-modified with hyaluronic acid (HA). This design enables CD44 receptor-mediated tumor targeting and tumor microenvironment-responsive degradation. Upon exposure to the mildly acidic and reductive tumor microenvironment, MnO2 is reduced to Mn2+, leading to glutathione depletion, in situ oxygen generation, and alleviation of hypoxia, while the released Mn2+ catalyzes Fenton-like reactions to generate highly toxic hydroxyl radicals (•OH) and simultaneously serves as an efficient T1-weighted MRI contrast agent. Meanwhile, TCPP enhances singlet oxygen (1O2) generation upon ultrasound activation, enabling effective sonodynamic therapy. Through the coordinated activation of SDT and CDT under MRI guidance, HA-MnO2-TCPP nanoparticles achieve precise and efficient tumor suppression without reliance on photothermal mechanisms. This work provides a robust paradigm for tumor microenvironment-responsive nanotheranostics and highlights the potential of ultrasound-activated strategies for precision cancer therapy.
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