JOURNAL OF APPLIED POLYMER SCIENCE
·
2024
·
IF 2.7
Xingtong Gao,Kai Liu,Wenjin Hu,Aixiang Li,Zijian Lyu,Haiqiang Chen,Hu Zhu
Currently, the incidence of kidney failure is increasing worldwide. Hemodialysis is the main method for the treatment of kidney diseases, and the continuous development of new hemodialysis membranes is the key to improve the treatment effect and the quality of life of patients. In this study, heparinized multi-walled carbon nanotube/cellulose acetate (Hep/MWCNTs@CA) membranes were prepared by non-solvent induced phase separation (NIPS). The morphology and pore structure of Hep/MWCNTs@CA membranes were characterized by scanning electron microscopy (SEM). The elongation at break and tensile strength of the membranes were characterized by a mechanical property universal tester. The results showed that mechanical properties of 0.1 wt% MWCNTs enhanced the membrane is best. The water contact angle test confirmed that MWCNTs can improve the membrane hydrophilicity, and combined with the adsorption test results for proteins, it was found that the hydrophilicity can reduce the adsorption of proteins to the composite membrane and improve the blood compatibility of the material. The dialysis performance of urea and lysozyme was tested by a self-made simulated hemodialysis device, and the clearance rates of urea and lysozyme were 84% and 47.2%, respectively, which showed that the interfacial gap between MWCNTs and CA matrix can make the composite membrane retain a high retention rate of macromolecular proteins (92.3%) and further improved the clearance rate of small and medium molecular toxins. In addition, the good blood compatibility and biocompatible properties of the materials were confirmed by hemolysis, recalcification and cytotoxicity tests on Hep/MWCNTs@CA membranes. In conclusion, the prepared Hep/MWCNTs@CA holds great potential for application in the field of hemodialysis.
研究领域:
材料科学
·
医学
·
生物工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2020
·
IF 7.183
Yang Liu,Guiliang Li,Qiu Han,Haibo Lin,Qiang Li,Jing Hua,Fu Liu
Inhibiting thrombosis in extracorporeal dialyzer is predominantly determined by the compatible interface design. It is challenging to develop anticoagulant dialyzers for clinical heparin free dialysis. Herein, we develop an environmentally friendly approach to construct a hydrogel skin composed of poly (sodium alginate-acrylic acid) P (SA-AA) network on polysulfone (PSf) membranes via pre-seeding initiator into membrane matrix and subsequently crosslinking SA and AA in aqueous solution. Physicochemical properties of modified membranes with different SA/AA ratio were characterized by attenuated total reflectance fourier transform infrared spectra (ATR-FTIR) spectrum, proton nuclear magnetic resonance ( 13 C NMR), scanning electron microscopy (SEM) and atomic force microscope (AFM) and contact angle. Antifouling property was demonstrated by proteins adsorption and E. coli adhesion. Hemocompatibility was comprehensively evaluated by hemolysis ratio, anticoagulation activity (APTT and TT), complement activation, platelets activation and contact activation. Anticoagulant dialyzer was fabricated via skin implantation strategy to implement a chronic renal swine dialysis with heparin free administration. Clotting was significantly inhibited during the whole dialysis session, moreover, the dialyzer showed improved toxins clearance, including urea (~185 mL/min), creatine (~153 mL/min), inorganic phosphorous (~120 mL/min) and β 2 -microglobulin (~110 mL/min). The modified membrane bearing abundant carboxylate groups contributes to excellent hydrophilicity and Ca 2+ chelation capability, which is considered to be the predominant anti-clotting mechanism via both intrinsic and extrinsic cascades. The anticoagulant PSf dialyzer demonstrates promising clinical application for heparin free dialysis.
研究领域:
生物医学工程
·
材料科学
·
血液透析
·
生物材料
✓ 已验证
产品关联
DOI
高影响因子
Nature Water
·
2025
·
IF 24.1
Chen Yuanmiaoliang,Wang Longchao,del Cerro Martina,Wang Li,Zhang Xuan,Elimelech Menachem,Wang Zhangxin
High-salinity organic wastewaters pose a major challenge for conventional wastewater treatment processes. Here we propose dialysis as an innovative solution to overcome this challenge. Dialysis uses an ultrafiltration (UF) membrane that allows the passage of salts while rejecting the organic substances, operating in a bilateral countercurrent flow mode without the application of hydraulic pressure. Using bench-scale experiments and a model for salt and water transport in leaky membranes, we demonstrate that dialysis can effectively desalinate high-salinity organic wastewaters without diluting the wastewater stream. By comparing the salt/organic selectivity of dialysis and UF using the same membrane, we show that dialysis can effectively fractionate salts and organic substances in high-salinity organic wastewaters. Additionally, we find that, unlike UF, dialysis is almost unaffected by membrane fouling, highlighting its excellent fouling resistance. We conclude by proposing potential high-salinity organic wastewater treatment schemes based on dialysis, paving the way for more sustainable and effective management of challenging wastewaters.
研究领域:
环境科学
·
水处理技术
·
膜分离技术
✓ 已验证
产品关联
DOI
高影响因子
AAPS PHARMSCITECH
·
2020
·Vol. 21
·期 5
·1-7
·
IF 2.401
Yan Na,Tang Zhan,Xu Yuqi,Li Xiumin,Wang Qiao
Ferulic acid is contained in some Chinese herbal medicines such as Ligusticum chuanxiong or Angelica sinensis. Studies have focused on the treatment of inflammatory diseases and pain using ferulic acid. However, little is known about its pharmacokinetics after transdermal administration. The present research investigated the pharmacokinetic behavior of ferulic acid in rat plasma and skin microdialysate after ferulic acid transdermal or intragastric administration. Samples collected at predetermined time points were determined by a simple and sensitive HPLC-UV method. The pharmacokinetic parameters were estimated using non-compartmental analysis with DAS 2.0 software. The values of AUC 0- t and C max after intragastric administration (20 mg/kg) in plasma were 281.47 ± 46.76 min mg/L and 12.20 ± 2.46 mg/L, respectively. After emulsion transdermal administration (117 mg/kg, 35 mg/4 cm 2 ), the values of AUC 0- t and C max in plasma and skin microdialysate were 953.90 ± 175.30 min mg/L, 7630.47 ± 1410.33 min mg/L, 3.00 ± 0.61 mg/L, and 19.08 ± 4.39 mg/L, respectively. Here, we show a promising delivery system for ferulic acid that could replace traditional administration, and a better understanding of the transdermal pharmacokinetics of ferulic acid, which may be helpful for further clinical and laboratory studies.
研究领域:
药理学
·
中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
·
2021
·Vol. 13
·期 13
·11499-11511
·
IF 4.987
Wang Wei,Wang Zhijuan,Li Kai,Liu Yuxin,Xie Delong,Shan Shaoyun,He Liang,Mei Yi
Numerous studies have shown that patients with COVID-19 have a high incidence of renal dysfunction. However, the dialysis supplies, including dialysates, are also severely inadequate in hospitals at the pandemic centers. Therefore, there is an urgent need to develop materials that can efficiently and rapidly remove toxins and thus regenerate dialysate to make this vital resource remains readily available. In this work, by simple carbonization and activation treatment, the porous activated carbon from waste rubber seed shell (RAC) was prepared. The adsorption results showed that the maximum adsorption capacities of the obtained RAC for creatinine and uric acid were 430 mg/g and 504 mg/g, respectively. Significantly, the adsorption process can be close to the equilibrium state within 0.5 h, which proved the ultra-fast adsorption response capacity of RAC. Further, the thermodynamics analysis results showed that both the creatinine and uric acid adsorption processes were monolayer, exothermic, and spontaneous. The adsorption kinetics results indicated that the adsorption process of the two uremic toxins followed the pseudo-second-order rate model and was dominated by chemisorption. The instrument analysis results reflected the efficient adsorption of the RAC for the above uremic toxins which might be due to the dipole–dipole interaction between the dipolar oxygen-containing groups of the surface of RAC and the dipoles of the toxins. Moreover, the formed hydrogen bonds between the oxygen groups and the toxins also played an important role. In all, the as-prepared RAC has the potential to efficiently remove major toxins from the dialysate and can be used in in vitro dialysis of numerous patients during the current COVID-19 pandemic.
研究领域:
医学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2023
·
IF 5.8
Lu Jin,Xiumin Li,Xu Chen,Xinghao Chen,Ye Liu,Haonan Xu,Qiao Wang,Zhan Tang
Puerarin (PUE), an isoflavonoid isolated from Pueraria lobata (Willd) Ohwi root, is a β -adrenergic receptor inhibitor used in treating glaucoma. The concentration range of gellan gum was determined based on the formulation viscosity and gelling capacity. PVP-K30 and gellan gum were used as variables, with the viscosity of formulation: STF = 40: 21, the 4 h permeation rate of rabbit isolated sclera, and 2 h in vitro release rate as response values. The JMP software was used to optimize the results, presenting that gellan gum was the main factor influencing viscosity. The in vitro release and permeation rate were primarily influenced by PVP-K30. The optimal prescription was 0.45% gellan gum and 6.0% PVP-K30. The in vitro release and permeation characteristics of puerarin in situ gel (PUE-ISG) were investigated using PUE solution as a control. The dialysis bag method results indicated that the release of the solution group leveled off after 4 h, while the PUE-ISG group had been continuously releasing. However, the cumulative release rates of the two were no longer significantly different at 10 h. The cumulative permeation rates of the ISG and solution groups were not significantly different ( P > 0.05) in the rabbit isolated sclera. The apparent permeability Papp and steady-state flux Jss of PUE-ISG were 0.950 ± 0.059 cm/h and 9.504 ± 0.587 mg·(cm·h), respectively. A sensitive and stable HPLC-MS/MS analytical method for quantifying aqueous humor concentrations of PUE was validated. A microdialysis technique was successfully used in the aqueous humor pharmacokinetics study to sample aqueous humor from rabbit eye continuously. The results revealed that PUE-ISG significantly increased the drug concentration in the aqueous humor, with C max and AUC (0-t) 3.77 and 4.40 times higher than those of the solution group, respectively. T max was also significantly prolonged, indicating good prospects for clinical application. The developed PUE-ISG preparation has the characteristics of rapid drug release and sustained permeation, and increase the drug concentration in aqueous humor, with all inactive ingredients remaining within the maximum allowable limits recommended by the FDA guideline.
研究领域:
药剂学
·
药物代谢动力学
·
眼科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Jiaze Yan,Chao Jiang,Jiqi Zheng,Ming Guo
Background Microdialysis is an in vivo technique for dynamically analyzing biochemical components in tissues and fluids. Traditional microdialysis systems are primarily designed for low molecular weight polar compounds, resulting in lower recoveries for hydrophobic compounds. This study aimed to develop a greener, cost-effective, and enhanced microdialysis method for recovering eugenol, isoeugenol, and methyl isoeugenol, utilizing hydrophobic deep eutectic solvents as the perfusate, with the method's feasibility verified through in vivo experiments. Results Among the 19 types of deep eutectic solvents evaluated, thymol-levulinic acid was identified as the most suitable for eugenol compounds based on liquid-liquid microextraction and microdialysis experiments, showing an in vitro microdialysis recovery approximately 100 times higher than that of water. The optimization of microdialysis parameters was conducted, including molar ratio of HBA:HBD, temperature, flow rate, and semi-permeable membrane length. In the in vivo microdialysis experiments, the temporal concentration of eugenol, which was used as an anesthetic in Glyptocidaris crenularis, was detected by GC-MS/MS and calibrated using ex vivo recovery. When the concentration of eugenol in artificial seawater was maintained at 100 μg mL −1 , the eugenol levels in the coelomic fluid of Glyptocidaris crenularis rose from 0 to 6.89 ± 0.38 μg mL −1 within 60 min, then stabilized in next 80 min. Significance The findings demonstrate that deep eutectic solvents enhanced microdialysis provides high recoveries and enables real-time monitoring of drug concentration changes in biological systems, making this method a valuable tool for pharmacokinetic studies and environmental monitoring.
研究领域:
分析化学
·
药理学
·
海洋生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2019
·
IF 7.015
Yang Liu,Qiu Han,Tiantain Li,Jing Hua,Fu Liu,Qiang Li,Gang Deng
Heparin free prescription is highly desired for haemodialysis. Versatile strategies have been developed to address the anti-coagulation modification of dialysis membranes. We here develop a facile and effective method to construct an anticoagulant coating on polymeric membranes or a dialyzer through hydrophobic-hydrophobic interaction. An amphiphilic heparin-like copolymer was first synthesized via a free radical polymerization based on alkyl chain lauryl methacrylate (LMA), acrylic acid (AA), and sodium 4-vinylbenze-sulfonate (SSNa) segments. The specific structure of terpolymer was characterized via Fourier transform infrared spectra (FTIR), Proton nuclear magnetic resonance ( 1 H NMR) spectra and size exclusion chromatography (SEC). The amphiphilic copolymer was anchored onto Polylactide (PLA) membrane surface from an aqueous-based solution via hydrophobic-hydrophobic interaction. An Attenuated total reflectance Fourier transform infrared spectra (ATR-FTIR), energy dispersive spectrometer (EDS) and X-ray photoelectron spectroscopy (XPS) confirmed the successful immobilization of heparin-mimicking terpolymers. The modified membranes showed excellent antifouling property and hydrophilicity. Moreover, the membranes exhibited outstanding haemocompatibility with suppressed platelet adhesion, low complement activation (C3a and C5a), expanded coagulation time and limited haemolysis ratio. The prolonged activated partial thromboplastin time and thrombin time (APTT>600 s, TT > 140 s) certified the excellent self-anticoagulant performances. To further examine the applicability and effectiveness, a polysulfone dialyzer was modified by this strategy. The ultrafiltration coefficient and clearance performances were examined. Overall, the hydrophobic-hydrophobic interaction strategy simplified the modification procedure of dialysis membrane, implying its great potential in heparin free dialysis application.
研究领域:
生物医学工程
·
材料科学
·
血液净化技术
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Lu Shi,Feifei Cao,Limin Zhang,Yang Tian
The development of switches responding to specific pH changes was particularly useful in wide application fields. Owing to flexible switches simulated by pH, i-motif DNAs are widely used as a pH sensor. But its character of structure transition strongly dependent on acidic pH severely hampers the application of i-motif DNA in physiological media. Herein, we report the stable i-motif structure formed at a physiological pH triggered by spatial confinement of silica nanochannels. Three classic DNA chains containing 21-mer i-motif domain base-pairs and a single-stranded multiply (T)n spacer, 5′-COOH-(T)n-CCCTAACCCTAACCCTAACCC-3′, were employed to evaluate the enhanced stability of i-motif structure. Compared to their free states in a dilute solution, the transition pH of all i-motif DNAs decorated in nanochannels remarkably shifts toward a neutral pH. Moreover, the transition midpoint can be tuned sensitively over the physiologically relevant pH range through slightly varying the length of T base spacer. Density functional theory (DFT) calculations validate that the increased proton density in a nanochannel triggers the formation of an i-motif structure under a neutral pH. Finally, this i-motif DNA based nanochannels electrode was successfully employed to monitor pH in brain microdialysates followed by cerebral ischemia. The present approach is not limited by fundamental investigation for DNA conformation but may extend toward the manipulation of i-motif based structures for artificial molecular machines and signaling systems.
研究领域:
生物化学
·
分子生物学
·
医学检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2023
·Vol. 140
·期 12
·e53640
·
IF 3
Zezhen Zhang,Shuman Feng,Qianyu Wei,Lili Wu
Low dialysis and blood incompatibility efficiency are key issues to be addressed for polymeric hemodialysis membranes. To improve the comprehensive performance of hemodialysis membranes, polyethersulfone (PES)/tannic acid (TA) coblended ultrafiltration membranes were prepared and modified with a heparin-like functionalized TA coating. The coblended TA improved the pore structure of the PES ultrafiltration membrane. And it could also undergo π-π conjugation with the heparin-like functionalized TA in the modified solution, resulting in a greater abundance of modified groups loaded on the membrane surface and pores close to the surface. The modified coating further improved the membrane performance. The physicochemical properties, solute filtration, and blood compatibility properties of the membrane were tested. The effect of TA on the pore structure of the membrane and the presence of modified layers were demonstrated by morphological and chemical structure analyses. The final modified membrane had an ultrahigh water flux (1053 L/m 2 ·h), improved dialysis performance (BSA retention >99%, Lysozyme clearance >30% and Urea clearance >90%), and excellent hemocompatibility (The hemolysis rate was 1.31%, and APTT, PT, and TT values were increased by 40.8%, 74.2%, and 85.9%, respectively). This study shows that TA has great potential for improving the pore structure of polymeric membranes.
研究领域:
材料科学
·
医学工程
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
Science of Advanced Materials
·
2020
·Vol. 12
·期 12
·1896-1906
·
IF 1.117
Che Lishuang,Chen Jinhai,Zhang Yun,Li Yuangen,Li Yueting
In this study, cellulose is used as the raw material. Considering that polyether sulfone has good biocompatibility and can reduce protein adsorption, the ionic liquid is used to dissolve, and dry-wet phase conversion method is used to blend cellulose and polyether sulfone to produce
the composite polymer hemodialysis membrane material. Then, an anticoagulant/hydrophilic heparinoid is synthesized and applied to the dialyzer made of composite polymer dialysis membrane material. During the preparation process, the spinning solution (SS) temperature, air gap (AG) length,
and coagulation bath (CB) temperature are changed, and their effects on the structure/performance of the dialysis membrane are analyzed. The test shows that the spinning liquid temperature shouldn’t exceed 100 °C, the AG length shouldn’t exceed 10.0 cm, and the CB temperature
at 30.6 °C could maximize the performance of the dialysis membrane. In the experiment, the new heparin is divided into three structural systems (M-1, M-3, M-1 / M-2), which are applied to the dialysis membrane to obtain three kinds of dialysis membrane (CP-A, CP-B, CP-C). The hydrophilic
groups on the surface of the composite polymer dialysis membrane are modified to form a hydration layer with water to avoid direct contact between proteins and the polymer dialysis membrane, and APTT test value reaches 600 s, which proves that the dialyzer designed with dialysis membrane material
in this study has a good anticoagulation effect.
研究领域:
生物材料学
·
医学工程
·
生物医学
✓ 已验证
DOI
高影响因子
Canadian Journal of Infectious Diseases & Medical Microbiology
·
2025
·Vol. 2025
·期 1
·5553355
·
IF 2.6
Kai Ming Chow,Siu Kwan Wo,Simon Wai Yin So,Phyllis Mei Shan Cheng,Keary Rui Zhou,Wai Li Lim,Joan Zhong Zuo,Philip Kam Tao Li
Background: With emerging antibiotic resistance, many patients on peritoneal dialysis require newer antibiotic treatment such as daptomycin. Inadequate clinical information exists across different peritoneal dialysis solutions, including icodextrin, for the stability of intraperitoneal daptomycin. To guide the clinical practice of intraperitoneal daptomycin treatment, we need to establish the stability of daptomycin at dextrose concentration higher than 1.5% and icodextrin, as well as the duration of stability. Methods: We tested the stability of daptomycin in three types of peritoneal dialysis bags (UltraBag dextrose 2.5%, UltraBag icodextrin 7.5%, and Stay-Safe Balance 2.3%). Daptomycin was reconstituted with water for injection (50 mg/mL), followed by administration to peritoneal dialysis bags to obtain the final daptomycin concentrations of 70 μg/mL (equivalent to 140 mg/2L, the maintenance level) and 245 μg/mL (equivalent to 490 mg/2L, the loading level). The bags were then placed at ambient temperature (25°C) followed by withdrawing 5 mL samples at 0, 4, 8, 12, 24, and 48 h for UltraBag dextrose 2.5% and UltraBag icodextrin 7.5% and 0, 4, 8, 12, and 24 h for Stay-Safe Balance 2.3%. The concentrations of daptomycin in the collected samples were quantified by high-performance liquid chromatography with diode array detector (HPLC-DAD). Results: Under ambient condition, daptomycin was stable at maintenance level in UltraBag dextrose 2.5% for 48 h and in UltraBag icodextrin 7.5% or Stay-Safe Balance 2.3% for 24 h. For loading level, daptomycin was stable in UltraBag dextrose 2.5% and Stay-Safe Balance 2.3% for 12 h and in UltraBag icodextrin 7.5% for 48 h. Conclusions: Current stability results support and guide the use of intraperitoneal daptomycin in different dialysis solutions. Patients with peritonitis requiring icodextrin exchange and assisted preparation of daptomycin can benefit from nurses who provide daily home visit based on our stability results.
研究领域:
药理学
·
临床医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）