Journal of Coatings Technology and Research
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2019
·Vol. 16
·期 3
·857-868
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IF 1.584
Peng Mengke,Zhang Xiaodan,Xiao Xiao,Dong Mengjin,Zhao Guowei,Liu Peng,Chen Yashao,Wang Changhao
Abstract Magnesium alloy (MgA) was widely used in biomedical field owing to its good biocompatibility and degradability. The surface of MgA was usually modified to improve its corrosion resistance, biocompatibility, and biological properties. Herein, we employed a layer-by-layer assembly technique to assemble both polyanionic and polycationic electrolytes onto the microarc oxidation-treated MgA surface to yield MgA-MgO-PEI-[Ge(HANPs)/Lzm] 50 , where the gelatin-conjugated hydroxyapatite nanoparticles [Ge(HANPs)] are the polyanionic electrolyte, lysozyme (Lzm) is the polycationic electrolyte, and polyethyleneimine (PEI) is the transition layer. The morphology and chemical composition of MgA-MgO-PEI-[Ge(HANPs)/Lzm] 50 were characterized by X-ray diffraction, scanning electron microscopy, and X-ray photoelectron spectroscopy, indicating that [Ge(HANPs)/Lzm] 50 were successfully fabricated on the surface of MgA-MgO. The surface of MgA-MgO-PEI-[Ge(HANPs)/Lzm] 50 exhibited good hydrophilicity as evidenced by the low water contact angle of 24.5°. Excellent corrosion resistance of MgA-MgO-PEI-[Ge(HANPs)/Lzm] 50 was obtained since it can decrease about four orders of magnitude of corrosive current ( I corr ) compared to pristine MgA. The biological assay for MgA-MgO-PEI-[Ge(HANPs)/Lzm] 50 showed good antiplatelet adhesion and excellent antibacterial activities against both E. coli and S. aureus. Graphical abstract
研究领域:
生物材料学
·
表面工程学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2020
·Vol. 12
·期 47
·5691-5698
·
IF 2.596
Wenxiang Xiao,Dongcan Zhi,Qiaoni Pan,Yongbo Liang,Fang Zhou,Zhencheng Chen
Bilirubin originates from hemoglobin metabolism and is an important biomarker for liver function. A ratiometric film sensor based on gold nanoclusters (AuNCs) was fabricated for highly sensitive determination of free bilirubin (fBR). Using bovine serum albumin (BSA) as a template, AuNCs that can emit blue and red fluorescence were prepared by the hydrothermal method at different pH values. Two kinds of AuNCs were incorporated into a single film by the layer-by-layer assembly (LBL) technique. The obtained thin-film showed dual fluorescence peaks excited at 372 nm, corresponding to the blue (443 nm) and red (622 nm) emissions of AuNCs respectively. When fBR interacted with the film, both fluorescence peaks were quenched at different degrees. A ratiometric method for fBR detection was established based on the fluorescence intensity ratio of the two emissions. The linear calibration curve for fBR lay in the concentration range of 0.01–2.00 μmol L−1 with a detection limit of 8.90 ± 0.34 nmol L−1 (S/N = 3). The film sensor showed a quick and sensitive response to fBR and could detect fBR in real samples with satisfactory results.
研究领域:
分析化学
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纳米技术
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生物传感器
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yuemei Zhang,Haoyue Ning,Jing Xu,Lixin Lu
This study focused on the encapsulation of modified halloysite nanotubes (AHNT) using sodium alginate (SA) and chitosan (CS) through a layer-by-layer assembly technique. The objective was to develop new composites (CPs) that could control the release of carvacrol (Car) and enhance its stability. The influence of various conditions on the microstructure, chemical properties, and slow-release effects of the CPs was examined by adjusting the pH and concentration of the CS solution. The results indicated that the CPs (CS4.5–1), created with a CS solution concentration of 1 mg/mL at a pH of 4.5, in conjunction with a 1 mg/mL SA solution, demonstrated a superior encapsulation structure and a loading efficiency of 26.33 %. In vitro release experiments confirmed that the CPs exhibited effective slow-release properties for Car. Furthermore, the CS4.5–1 composite provided a physical barrier that resulted in the retention of 94.90 % and 83.61 % of Car after 6 h of exposure to UV light and heat, respectively. Antimicrobial and antioxidant assays indicated that CS4.5–1/Car possessed significant antioxidant properties and effectively inhibited the growth of E. coli and S. aureus . Consequently, the prepared CPs have the potential to enhance the bioactivity of active compounds and may be beneficial for food preservation and other applications.
研究领域:
纳米技术
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材料科学
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食品科学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Yonglin Yu,Qichun Ran,Xinkun Shen,Hong Zheng,Kaiyong Cai
After implantation into a host, titanium (Ti) orthopaedic materials are facing two major clinical challenges: bacterial infection and aseptic loosening, which directly determine the long-term survival of the implant. To endow Ti implant with self-defensive antibacterial properties and desirable osteo/angio-genic differentiation potentials, hyaluronic acid (HA)-gentamicin (Gen) conjugates (HA-Gen) and chitosan (Chi) polyelectrolyte multilayers were constructed on deferoxamine (DFO) loaded titania nanotubes (TNT) substrates via layer-by-layer (LBL) assembly technique, termed as TNT/DFO/HA-Gen. The HA-Gen conjugate was characterized by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance ( 1 H NMR). The physicochemical properties of the substrates were characterized by field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and contact angle measurements. The on-demand DFO release was associated with the degradation of multilayers triggered by exogenous hyaluronidase, which indicated enzymatic and bacterial responsiveness. The TNT/DFO/HA-Gen substrates displayed effective antifouling and antibacterial properties against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ), while were favourable for the adhesion, proliferation and osteo/angio-genic differentiation of mesenchymal stem cells (MSCs). The multifaceted drug-device combination (DDC) strategy showed potential applications in orthopaedic fields.
研究领域:
生物材料
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组织工程
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骨科
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药剂学
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生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2023
·
IF 5.9
Xie Fu,Hui Tong,Yutong Wu,Kun Zhang,Xiong Sheng,Lyes Douadji,Guotian He,Zicheng Qiu,Baoping Zhou,Shuai Kang,Jinling Luo,Ziwei Pan,Wenqiang Lu
Flexible wearable sensors with high sensitivity, broad sensing range, and low detection accuracy have great potential applications in human motion online monitoring, human healthcare detection, and human–machine interface. However, high sensitivity and broad sensing range conflict, as the former requires conspicuous structural changes under microstrain while the latter requires complete morphology under large deformation. Herein, we report few-layer 2D Ti3C2Fx and 1D polypyrrole (PPy) nanowires with a sandwich stack structure transfer on polyacrylic acid (PAA) tape based on layer-by-layer (LBL) assemble technology. The 1D PPy nanowires enhance the strain sensor’s interfacial adhesion and electron transmission channel, resulting in high sensitivity and a permissible detection range (0–50%). Under various applied force stimuli, the synergetic effect of Ti3C2Fx nanosheets (4.72 nm thickness) and PPy nanowires (diameter 20–50 nm) endows the stack structure with good electrical–mechanical performance, which is reflected by the sensor’s high sensitivity (GF = 2950, ε = 50%; GF = 475, ε = 10%; GF = 22, ε = 0.1%). Such a flexible sensor with a permissible detection range (up to 50%), a low detection limit (0.1%), and reliable repeatability (>1500 cycles) has potential applications for human motion detection, clinical diagnosis, and healthcare monitoring.
研究领域:
材料科学
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纳米技术
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可穿戴技术
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）