PEST MANAGEMENT SCIENCE
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2020
·Vol. 76
·期 11
·3792-3799
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IF 3.75
Xiujun Tang,Fengjiao Hao,Huizhu Yuan,Xiaojing Yan,Daibin Yang,Alan G Taylor
BACKGROUND A systemic seed treatment can be taken up into shoot tissues during early corn seedling growth. However, the pathway that a systemic compound is taken up, either from seed or root uptake to shoot tissues is not fully understood. To study the single contributions of seed pathway and root pathway for the uptake and translocation of imidacloprid seed treatment, two methods were developed: A seed treatment method and a growing media/hydroponic solution delivery method. The seed treatment method employed a live and dead seed separated with plastic wrap to provide a barrier. The growing media/hydroponic solution delivery method quantified the capacity for uptake by seeds and roots. RESULTS The seed pathway transported 1.9–2.5 times more imidacloprid into shoot tissues when compared to the root pathway at the same dosage rate. The higher amount of imidacloprid taken up and translocated by the seed pathway was attributed to the fact that the corn seeds were in direct contact with high provided concentrations of imidacloprid. However, the root pathway showed 7.9–9.5 times higher capacity for transporting imidacloprid into shoot tissues when compared with the seed pathway. Whenever imidacloprid was taken up by seed or root, amounts of imidacloprid were measured in both tissues of seed and root. CONCLUSION The seed pathway transported more imidacloprid than the root pathway during early seedling growth since corn seeds were contacted by higher concentrations of imidacloprid. Both seed pathway and root pathway were occurring concurrently during early seedling growth of corn. © 2020 Society of Chemical Industry
研究领域:
植物生理学
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农药科学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Jiaxin Yu,Wei Qiu,Xinchen Lin,Yishi Wang,Xiaohui Lu,Yongbo Yu,Haiteng Gu,Song Heng,Haochen Zhang,Jun Ma
Nowadays, the limited catalytic efficiency, metal leaching, and stability decrease during catalyst reuse hinder the heterogeneous catalysis application in water treatment. Moreover, the heterogeneous catalysis mechanism regarding novel sodium periodate (NaIO 4 )-based advanced oxidation process (AOP) is ambiguous. Herein, MgMn 2 O 4 spinel was synthesized through the co-precipitation method to test the NaIO 4 -based AOP’s mechanism. The morphological and physicochemical properties of MgMn 2 O 4 were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). In this research, 0.1 g L −1 MgMn 2 O 4 activated 1 mM NaIO 4 to remove 10 μM bisphenol A (BPA) in 60 min without toxic iodine species generation and the acute toxicity decrease through radical (iodine active substances, IO 3 • ) and non-radical ( 1 O 2 , Manganese (IV)-oxo species) pathways by utilizing X-ray photoelectron spectroscopy (XPS), electron paramagnetic resonance (EPR), radical quenching experiments, and methyl phenyl sulfoxide (PMSO)-based probing experiment. With tetrahedral Mg 2+ introduction and the Mn 2+ /Mn 3+ /Mn 4+ redox domination, the MgMn 2 O 4 shows high catalytic ability for NaIO 4 . To summarize, this research would extend the mechanism for the NaIO 4 -based advanced oxidation process.
研究领域:
环境科学
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催化化学
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水处理技术
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DOI
高影响因子
PLANTA
·
2021
·Vol. 253
·期 5
·1-8
·
IF 4.116
Fu Jinqiu,Xu Wenbo,Huang Wei,Wang Bipeng,Li Shengchun,Zhang Jiang,Chang Ling
Main conclusion Importation of taxadiene synthase into chloroplasts is important for the efficient heterologous production of taxadiene. Abstract Taxadiene, the first committed precursor to taxol, is synthesized from geranylgeranyl pyrophosphate (GGPP) by action of taxadiene synthase (TS). Heterologous production of taxadiene could potentially rely on both cytosolic mevalonic acid (MVA) pathway and the plastidic methylerythritol phosphate (MEP) pathway. We suggest the compartmentalized engineering in chloroplast as an efficient approach for taxadiene production. In this study, we directly introduced the TS gene from Taxus brevifolia into the tobacco chloroplast genome and found that the transplastomic plants accumulated a low content of taxadiene, ~ 5.6 μg/g dry weight (DW). Moreover, we tried a combination of MEP and MVA pathways for taxadiene synthesis by nuclear transformation with a truncated version of TS (without encoding a transit peptide) into the transplastomic plants. However, this did not further improve the taxadiene production. In contrast, we found that taxadiene could be produced up to 87.8 μg/g DW in leaves of transgenic plants expressing TS with a chloroplast transit peptide, which was significantly higher than that in leaves of transplastomic plants. Thus, this study highlights the importance of TS importation into chloroplast for production of taxadiene.
研究领域:
植物学
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代谢工程
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生物化学
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PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2020
·Vol. 27
·期 17
·21924-21938
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IF 3.056
Feng Yongyong,Zhou Aiguo,Zhang Yue,Liu Shulin,Pan Zhengkun,Zou Jixing,Xie Shaolin
Widely distributed western mosquitofish ( Gambusia affinis ) has been used as a new model species for hazard assessment of environmental stressors such as polycyclic aromatic hydrocarbons (PAHs). However, most of the PAH studies using G. affinis rely on targeted biomarker-based analysis, and thus may not adequately address the complexity of the toxic mechanisms of the stressors. In the present study, the whole transcriptional sequencing of G. affinis liver after exposure to a PAH model, benzo[a]pyrene (BaP) (100 μg/L), for 20 days was performed by using the HiSeq XTen sequencers. In total, 58,156,233 and 51,825,467 clean nucleotide reads were obtained in the control and BaP-exposed libraries, respectively, with average N50 lengths of 1419 bp. In addition, after G. affinis was exposed for 20 days, 169 genes were upregulated, and 176 genes were downregulated in liver. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were applied to all the genes to determine the genes’ biological functions and processes. The results clearly showed that the differentially expressed genes were mainly related to immune pathways and metabolic correlation pathways. Interestingly, almost all the pathways related with the immunity were upregulated, while the metabolism pathways were downregulated. Lastly, quantitative real-time PCR (qRT-PCR) was performed to measure expressional levels of twelve genes confirmed through the DGE analysis. These results demonstrate that BaP damages immunity and enhances the consumption of all available energy storage to activate mechanisms of the detoxification in G. affinis . Up until now, the present study is the first time that a whole transcriptome sequencing analysis in the liver of G. affinis exposed to BaP has been reported.
研究领域:
环境毒理学
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分子生物学
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鱼类生态学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2021
·
IF 9.642
Jiayu Niu,Xiufen Li,Xiguang Qi,Yueping Ren
With increased interest in the biodegradation of lignin, there is a pressing need to evaluate the feasibility of using microorganisms for lignin degradation. A novel Bacillus strain was separated from compost and identified as Brevibacillus thermoruber . B. thermoruber showed excellent performance in lignin degradation and degraded 81.97% of lignin after 7 d, which was similar to the lignin degradation rate of fungi. The biodegradation of lignin G and H monomers mainly proceeded via the β -ketoadipate pathway at 37 °C. At 55 °C, the degradation product of lignin S monomer was mainly a benzoic acid substance, indicating that the lignin was degraded via the benzoic acid pathway. The degradation products of lignin are important precursors for humus formation in compost. The results of this study provide new insights into the biodegradation pathway of lignin in different stages of composting.
研究领域:
微生物学
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环境科学
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2016
·
IF 2.857
Chongchong Sun,Si Chen,Yujian Jin,Hao Song,Songlin Ruan,Zhengwei Fu,Muhammad Asad Ullah Asad,Haifeng Qian
Photosynthesis is a very important metabolic pathway for plant growth and crop yield. This report investigated the effect of the herbicide imazethapyr on photosynthesis in the Arabidopsis thaliana pnsB3 mutant (a defect in the NDH pathway) and pgr5 mutant (a defect in the PGR5 pathway) to determine which cyclic electron transport chain (CET) of the NDH and PGR5 pathways is more important for protecting the photosynthetic system under herbicide stress. The results showed that 20 μg/L imazethapyr markedly inhibited the growth of the three ecotypes of A. thaliana and produced more anthocyanins and reactive oxygen species (ROS), particularly in the pgr5 mutant. The chlorophyll fluorescence results showed that PSII was severely damaged in the pgr5 mutant. Additionally, the CET was significantly stimulated to protect the photosynthetic system from light damage in Wt and the pnsB3 mutant but not the pgr5 mutant. The real-time PCR analysis indicated that imazethapyr treatment considerably decreased the transcript levels of most photosynthesis-related genes in the three treated groups. Several genes in the PGR5 pathway were significantly induced in the pnsB3 mutant, but no genes in the NDH pathway were induced in the pgr5 mutant. The gene transcription analysis showed that the pgr5 mutant cannot compensate for the deficit in the PGR5 pathway by stimulating the NDH pathway, whereas the pnsB3 mutant can compensate for the deficit in the CET cycle by regulating the PGR5 pathway. The iTRAQ analyses also showed that the photosynthesis system, glycolysis, and TCA cycle suffered the most severe damage in the pgr5 mutant. All of these results showed that the PGR5 pathway is more critical for electron transfer around PSI than the NDH pathway to resist herbicide stress.
研究领域:
植物学
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分子生物学
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药理学
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DOI
PubMed
高影响因子
CHEMOSPHERE
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2023
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IF 8.8
Jieli Ou,Jiewen Deng,Zhenran Wang,Yongsheng Fu,Yiqing Liu
A Cu(II)/heat coactivated peracetic acid (PAA) system for enhancing diclofenac (DCF) degradation was proposed in this work. The superiority of this synergetic activation strategy for PAA, working reactive species, catalytic mechanism and effects of reaction parameters on DCF elimination in this system were simultaneously investigated. Based on our results, the DCF loss rate in Cu(II)-heat/PAA process at pH 8.0 was about 49.3 and 4.2 times of that in Cu(II)/PAA and heat/PAA processes, respectively. Increasing the reaction temperature to 60 о C not only motivated the conversion of Cu(II) to Cu(I) but also facilitated the one-electron transfer between Cu(I) and PAA, boosting the generation of radicals. Organic radicals (mainly CH 3 C(O)O• and CH 3 C(O)OO•) were evidenced to be the core oxidizing substances dominating in the destruction of DCF while hydroxyl radical (•OH) made a minor contribution in this system by electron paramagnetic resonance (EPR) method together with scavenging experiments. This study broads the eyes into enhanced PAA activation initiated by homogenous Cu(II), providing a simple but efficient tool to degrade micropollutants .
研究领域:
环境科学
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化学工程
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DOI
PubMed
高影响因子
Synthetic and Systems Biotechnology
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2024
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IF 4.4
Shanquan Liang,Zi He,Dan Liu,Shaoqing Yang,Qiaojuan Yan,Zhengqiang Jiang
2′-Fucosyllactose (2′-FL) is one of the important functional oligosaccharides in breast milk. So far, few attempts on biosynthesis of 2′-FL by the salvage pathway have been reported. Herein, the salvage pathway enzyme genes were introduced into the E. coli BL21star(DE3) for synthesis of 2′-FL. The 2′-FL titer increased from 1.56 to 2.13 g/L by deleting several endogenous genes on competitive pathways. The α-1,2-fucosyltransferase (WbgL) was selected, and improved the 2′-FL titer to 2.88 g/L. Additionally, the expression level of pathway enzyme genes was tuned through optimizing the plasmid copy number. Furthermore, the spatial distribution of WbgL was enhanced by fusing with the MinD C-tag. After optimizing the fermentation conditions, the 2′-FL titer reached to 7.13 g/L. The final strain produced 59.22 g/L of 2′-FL with 95% molar conversion rate of lactose and 92% molar conversion rate of fucose in a 5 L fermenter. These findings will contribute to construct a highly efficient microbial cell factory to produce 2′-FL or other HMOs.
研究领域:
生物化学
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微生物学
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合成生物学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Ma Bai,Youzheng Chai,Anwei Chen,Jihai Shao,Shiye Zhu,Jiayi Yuan,Zhenghang Yang,Jiahao Xiong,Doudou Jin,Keqi Zhao,Yanziyun Chen
The extensive utilization of neonicotinoid insecticides (NNIs) in agricultural practices ultimately poses a significant threat to both the environment and human health. This work focuses on the efficient degradation and detoxification of the representative NNI, thiamethoxam (THX), and explores the underlying mechanism using a Co-Fe-Mn mixed spinel doped carbon composite catalyst activated persulfate. The findings demonstrate that the composite effectively degrades THX, achieving a degradation rate of 95% in 30 mins, while requiring only a fraction (one-sixteenth) of the oxidant dosage compared to pure carbon. The study aimed to examine the negative impact of reactive halogens on reactive oxygen species within a saline environment. The degradation byproducts were linked to the presence of two common electron-withdrawing groups, namely halogens and nitro in the THX molecule. It was hypothesized that the degradation process was primarily influenced by C-N bond breaking and hydroxylation occurring between the diazine oxide and 2-chlorothiazole rings. Consequently, dehalogenation and carbonylation processes facilitated the elimination of halogenated components and pharmacophores from the THX, leading to detoxification. In addition to the identified free radical pathway including SO 4 • − , •OH and O 2 • − contributed to THX degradation, the participation of non-radical pathways ( 1 O 2 and electron transfer) were also confirmed. The efficacy of detoxification was further validated through toxicity assessment, employing quantitative conformation relationship prediction and microbial culture utilizing Bacillus subtilis .
研究领域:
环境科学
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农药化学
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催化材料科学
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产品关联
DOI
PubMed
高影响因子
BIODEGRADATION
·
2023
·Vol. 34
·期 4
·371-381
·
IF 3.6
Yue Caixu,Jia Nan,Lv Xueru,Wang Shenghui
3, 5, 6-Trichloro-2-pyridinol (TCP) is a metabolite of the insecticide chlorpyrifos and the herbicide triclopyr, and it is higher toxic than the parent compounds. Microbially-mediated mineralization appears to be the primary degradative pathway and the important biological process of detoxification. However, little information is available on TCP complete metabolic pathways and mechanisms. In this study, the degradation of TCP was studied with a novel strain Micrococcus luteus ML isolated from a stable TCP degrading microbiota. Strain ML was capable of degrading 61.6% of TCP (50 mg/L) and 35.4% of chlorpyrifos (50 mg/L) at 24 h and 48 h under the optimal conditions (temperature: 35 °C; pH: 7.0), respectively. It could also degrade 3, 5-dichloro-2-pyridone, 6-chloropyridin-2-ol, 2-hydroxypyridine and phoxim when provided as sole carbon and energy sources. Seven TCP intermediate metabolites were detected in strain ML and two possible degradation pathways of TCP were proposed on the basis of LC–MS analysis. Both the hydrolytic-oxidative dechlorination pathway and the denitrification pathway might be involved in TCP biodegradation by strain ML. To the best of our knowledge, this is the first report on two different pathways responsible for TCP degradation in one strain, and this finding also provides novel information for studying the metabolic mechanism of TCP in pure culture.
研究领域:
环境微生物学
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生物修复
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代谢组学
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DOI
PubMed
高影响因子
Journal of Water Process Engineering
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2024
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IF 6.3
Song He,Jianxu Gao,Xin Jin,Saiping Guo,Yajun Huang
Sulfate radical-based advanced oxidation technologies (SR-AOPs) are extensively employed in the degradation of diverse wastewater pollutants owing to their superior capacity for degrading emerging and recalcitrant pollutants and their excellent adaptability. However, how to reduce the secondary pollution caused by metal ion spillage is still a major problem. In this work, Co 2+ was introduced into the molding process of SiO 2 microspheres as a novel activator of per-oxomonosulfate (PMS), which 15 mg of the catalyst completely degraded 100 mL of 40 mg/L of Rhodamine B (RhB) in 10 min, with a low Co leaching of only 0.18 mg/L. The effects of the introduction of Co, the addition of polyvinylpyrrolidone (PVP), H 2 O, and PMS, and the adjustment of pH, temperature, and anionic inorganics on the degradation of RhB were investigated. Based on the PMS activation mechanism analysis, it was concluded that the degradation of RhB involves two pathways, including the non-radical pathway dominated by 1 O 2 and mediated electron transfer and the radical pathway dominated by SO4 − , OH, and O2 − . This study presents a novel hybrid catalyst with high efficiency and easy recyclability, good recyclability, and low secondary pollution, which provides valuable insights into the field of wastewater treatment.
研究领域:
环境科学
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化学工程
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
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IF 15.1
Wei Chen,Xinning Dai,Zhujun Liu,Bin Du,Xinyu Zheng,Dandan Ma,Xianbin Huang
Periodate-based advanced oxidation processes (PI-AOPs) exhibited a promising future for the reduction of emerging refractory organic pollutants . However, the underlying mechanism and the sulfuretted effect of sulfide-modified metal silicate materials for PI activation were still unclear. In this study, sulfide-modified metal silicates (S-CoSi) were first synthesized and applied in the PI-AOPs system. Different from traditional metal silicate materials, sulfurization improved the catalytic performance. The enhanced degradation performance was from the increase in specific surface area and charge transfer efficiency, which derived from the sulfurization in metal silicate. DFT calculations revealed that the enhanced charge densities of Co-S bonds promoted the existence of Co (Ⅱ), which was more conducive to PI activation. Experimental and calculation results further demonstrated that Co was the active site in S-CoSi, and sulfur species strengthened the radical pathway by assisting Co (Ⅱ) regeneration (electron transfer from S to Co) rather than participating in the PI activation. Moreover, sulfurization promoted the generation of 1 O 2 (non-radical pathway) by increasing oxygen vacancy in the metal silicate. This study provided new insights into the effect of sulfurization on the radical and non-radical pathways in the metal silicates PI system.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）