Tan Ye,Muyuan Chai,Zhenxing Wang,Tingru Shao,Ji Liu,Xuetao Shi
Three-dimensionally printed (3DP) hydrogel-based vascular constructs have been investigated in response to the impaired function of blood vessels or organs by replicating exactly the 3D structural geometry to approach their function. However, they are still challenged by their intrinsic brittleness, which could not sustain the suture piercing and enable the long-term structural and functional stability during the direct contact with blood. Here, we reported the high-fidelity digital light processing (DLP) 3D printing of hydrogel-based vascular constructs from poly(vinyl alcohol)-based inks, followed by mechanical strengthening through engineering the nanocrystalline domains and subsequent surface modification. The as-prepared high-precision hydrogel vascular constructs were imparted with highly desirable mechanical robustness, suture tolerance, swelling resistance, antithrombosis, and long-term patency. Notably, the hydrogel-based bionic vein grafts, with precise valve structures, exhibited excellent control over the unidirectional flow and successfully fulfilled the biological functionalities and patency during a 4-week implantation within the deep veins of beagles, thus corroborating the promising potential for treating chronic venous insufficiency.
Zihao Wang,Mengyu Li,Bin Wang,Yin Xu,Jinyu Li,Shengmin Zhang,Qinghua Qin,Jianglin Wang
Clinical replacement of small-diameter vascular grafts (SDVG) for vessel lesion often encounters highly occluded risk due to acute platelet activation in a short run and poor endothelialization. Recent strategies built with either biochemical modification of heparinization or biophysical manipulation of hemodynamics have attempted to address the issue of unqualified long-term patency rate, but the major challenges on suppressing thrombosis and elevating fast endothelialization are yet untangled. Herein, we designed a biomimetic SDVG made of biochemical regulation of dopamine-mediated copper ion (DA-Cu II ) and biophysical regulation of intimal oriented nanotopography. The DA-Cu II system can significantly modulate the cell fates of vascular cells containing platelets, endothelial cells, and smooth muscle cells via catalytically producing nitric oxide (NO) in situ and the intimal oriented nanotopography can effectively promote the oriented growth and elongation of endothelial cells to achieve luminal remodeling and maintain an instructive long-term patency. Consequently, the current work integrates biochemical cue of NO signaling molecule with biophysical stimulation of intimal oriented nanotopography to create an off-the-shelf SDVG with specific anti-thrombosis and rapid endothelialization.
危险品化学品经营许可证（带存储）