INSECT MOLECULAR BIOLOGY
·
2020
·Vol. 29
·期 5
·444-451
·
IF 2.533
L. Guo,Z.-D. Zhou,F. Mao,X.-Y. Fan,G.-Y. Liu,J. Huang,X.-M. Qiao
Drosophila suzukii (spotted wing drosophila) has become a major invasive insect pest of soft fruits in the America and Europe, causing severe yield losses every year. The female D. suzukii shows the oviposition preference for ripening or ripe fruit by cutting the hard skin with its serrated ovipositor. A recent study reported that mechanosensation is involved in the texture discrimination during egg-laying behaviour in D. suzukii . However, the underlying mechanism and molecular entity that control this behaviour are not known. The transient receptor potential (TRP) channels and degenerin/epithelial sodium channels (DEG/ENaC) are two candidate gene families of mechanically activated ion channels. Thus, we first identified TRP and DEG/ENaC genes in D. suzukii by bioinformatic analysis. Using transcriptome sequencing, we found that many TRP genes were expressed in the ovipositor in both D. suzukii and D. melanogaster , while some DEG/ENaCs showed species-specific expression patterns. Exposure to drugs targeting TRP and DEG/ENaC channels abolished the oviposition preference for harder texture in female D. suzukii . Therefore, mechanosensitive ion channels may play significant roles in the texture assessment of egg-laying behaviour in D. suzukii , which has promising implications to further research on the development of novel control measures.
研究领域:
昆虫学
·
分子生物学
·
行为生态学
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产品关联
DOI
PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2024
·
IF 6.9
Ying Xun Zhou,Wen Ping Wang,Jin Ke,Hui Ping Ou,Lin Yun Chen,An Guo Hou,Peng Li,Yun Shu Ma,Wen Bin Jin
Dysfunction of the Nav1.5, Cav1.2, and Kv channels could interfere with the AP and result in arrhythmias and even heart failure. We herein present a novel library of nuciferine analogs that target ion channels for the treatment of arrhythmias. Patch clamp measurements of ventricular myocytes revealed that 6a dramatically blocked both the I Na and I Ca without altering the current voltage relationship (including the activation potential and peak potential), accelerated the inactivation of Nav and Cav channels and delayed the resurrection of these channels after inactivation. Additionally, 6a significantly decreased the APA and RMP without affecting the APD30 or APD50. The IC 50 values of 6a against Nav1.5 and Cav1.2 were 4.98 μM and 4.62 μM, respectively. Furthermore, 6a (10 μM) blocked I Ks , I K1 , and I to with values of 17.01 %±2.54 %, 9.09 %±2.78 %, and 11.15 %±3.52 %, respectively. Surprisingly, 6a weakly inhibited hERG channels, suggesting a low risk of proarrhythmia. The cytotoxicity evaluation of 6a with the H9c2 cell line indicated that this compound was noncytotoxic. In vivo studies suggested that these novel nuciferine analogs could shorten the time of arrhythmia continuum induced by BaCl 2 and normalize the HR, QRS, QT and QTc interval and the R wave amplitude. Moreover, 6a dose-dependently affected aconitine-induced arrhythmias and notably improved the cumulative dosage of aconitine required to evoke VP, VT, VF and CA in rats with aconitine-induced arrhythmia. In conclusion, nuciferine analogs could be promising ion channel blockers that could be further developed into antiarrhythmic agents.
研究领域:
药理学
·
心血管病学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Qi Liu,Shushu Ding,Rui Gao,Guoyue Shi,Anwei Zhu
ATP-sensitive potassium (K ATP ) channels couple intracellular metabolism to the electrical activity by regulating K + flux across the plasma membrane, thus playing an important role in both normal and pathophysiology. To understand the mechanism of ATP regulating biological ion channels, developing an ATP-responsive artificial nanochannel is an appealing but challenging topic because K ATP channel is a heteromultimer of two subunits (potassium channel subunit (Kir6.x) and sulfonylurea receptor (SUR)) and exhibit dynamic functions with adjustability and reversibility. Inspired by the structure of K ATP channels, we designed a smart copolymer modified nanochannel that may address the challenge. In the tricomponent poly( N -isopropylacrylamide) (PNIPAAm, PNI)-based copolymer system, phenylthiourea was used to bind the phosphate units of nucleotides and phenylboronic acid was introduced to combine the pentose ring of the nucleoside unit. Besides, a –COOH group with electron-withdrawing property was added into the phenylthiourea units, which may promote the hydrogen-bond-donating ability of thiourea . Specially, the smart copolymer not only provided static binding sites for recognition but also translated the recognition of ATP into their dynamic conformational transitions by changing the hydrogen-bonding environments surrounding PNIPAAm chains, thus achieving the gating function of nanochannel, which resembled the integration and coordination of Kir6.x and SUR units in active K ATP . The ATP-regulated ion channel exhibited excellent stability and reversibility. This study is the first example showing how to learn from nature to assemble the ATP-responsive artificial nanochannel and demonstrate the possible mechanism of ATP gating.
研究领域:
生物化学
·
材料科学
·
分子生物学
✓ 已验证
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Yong Wen,Jiewei Tan,Quanpei Xie,Zheng Ren,Yang Yang,Jinxin Liu,Fuqiang Xu,Shenglong Zhang,Si Cheng
Hydrogel evaporators with combined vertical/radial channel are demonstrated to synergistically leverage the advantages of each individual structural mode to achieve superior evaporation performance compared to single-structured counterparts. However, in such composite structures, the dimensions of micro- and macro-scale channels simultaneously influence both water transport and thermal transfer, and the underlying mechanisms governing the balance between water supply and heat loss remain insufficiently understood. Herein, a series of composite alkali-treated polyacrylonitrile/MXene@sodium alginate (PMS) hydrogel evaporators featuring controllable vertical channel dimensions is fabricated. Through adjusting the dimensions of interior channels of evaporators, it is investigated how varying the vertical channel size affects convective water flow, heat confinement, and overall evaporation performance. The systematic experiments and numerical simulations show that vertical microchannels significantly enhance convective water transport, while the surrounding radial structure effectively confines heat. Under one-sun illumination, this optimized vertical/radial composite achieves an evaporation rate of 5.01 kg m −2 h −1 at 165.13% energy efficiency. Further upscaling the microchannel to the macroscale amplifies convective effects, boosting the evaporation rate to 8.60 kg m −2 h −1 . These findings highlight the importance of finely balancing micro- and macro-scale channel architectures to simultaneously optimize water supply and heat loss, providing new guidelines for designing high-performance hydrogel evaporators.
研究领域:
可再生能源
·
材料科学
·
化学工程
·
纳米技术
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产品关联
DOI
高影响因子
ADVANCED MATERIALS
·
2022
·Vol. 35
·期 2
·2207141
·
IF 32.086
Yongsong Luo,Xiaoliang Chen,Xiangming Li,Hongmiao Tian,Sheng Li,Liang Wang,Juan He,Zhengbing Yang,Jinyou Shao
Developing highly sensitive strain sensors requires conduction pathways capable of rapidly switching between disconnection and reconnection in response to strain. Ion channels in living organisms exactly control the channel switch through protein-composed gates, achieving changeable ion currents. Herein, inspired by the gating characteristics of the ion channels, a programmable fluidic strain sensor enhanced by gating ion pathways through heterogeneous strain distribution of discrete micropillars is proposed. During stretching, the contraction and closure of the widthwise gaps between discrete micropillars greatly weaken or even nearly cut off the conduction pathway, resulting in orders of magnitude increase in resistance and thus ultrahigh sensitivity. By adjusting the combination form and structural parameters of the discrete micropillars in the fluidic channel, the sensitivity and strain range can be customized. Thus, a gauge factor of up to 45 300 and a stretch range of 590% are obtained. Benefiting from the fluidic gating mechanism, no mechanical mismatch can be observed at the interface, breaking through the sensing stability issue of flexible sensors. The proposed sensor can be used to detect the full range of human motion, and integrated into a data glove to achieve human–machine interaction.
研究领域:
材料科学
·
生物医学工程
·
柔性电子
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Chengning Tang,Yuying Zhu,Haoyu Bai,Guoqiang Li,Jiasong Liu,Weiming Wu,Yi Yang,Sensen Xuan,Huan Yin,Zuqiao Chen,Lin Lai,Yuegan Song,Moyuan Cao,Bensheng Qiu
Spontaneous separation of immiscible organic droplets has substantial research implications for environmental protection and resource regeneration. Compared to the widely explored separation of oil–water mixtures, there are fewer reports on separating mixed organic droplets on open surfaces due to the low surface tension differences. Efficient separation of mixed organic liquids by exploiting the rapid spontaneous transport of droplets on open surfaces remains a challenge. Here, through the fusion of inspiration from the fast droplet transport capability of Sarracenia trichome and the asymmetric wedge channel structure of shorebird beaks, this work proposes a spine with hierarchical microchannels and wedge channels (SHMW). Due to the synergistic effect of capillary force and asymmetric Laplace force, the SHMW can rapidly separate mixed organic droplets into two pure phases without requiring additional energy. In particular, the self-spreading of the oil solution on the open channel surface is utilized to amplify the surface energy difference between two droplets, and SHMW achieves the pickup of oil droplets floating on the surface of the organic solution. The maximum separation efficiency on 3-SHMW can reach 99.63%, and it can also realize the antigravity separation of mixed organic droplets with a surface tension difference as low as 0.87 mN·m–1. Furthermore, SHMW performs controllable separation, oil droplet pickup, and continuous separation and collection of mixed organic droplets. It is expected that this cooperative structure composed of hierarchical microchannels and wedge channels will be realized in resource recovery or chemical reactions in industrial production processes.
研究领域:
材料科学
·
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLANTA MEDICA
·
2016
·Vol. 83
·期 07
·624-630
·
IF 1.99
Yi-Lin Zhang,Jia-Wen Xu,Xiong-Hao Wu,Peng-Xiao Chen,Fang Wei,Dong-Dong Gao,Xiao-Wen Chen,Yu-Li Luo,Yun-Xin Zhu,Jiehong Huang,Ya-Xia Tan,Lei Zhao,Wen-Liang Zhou
Sodium tanshinone IIA sulphonate, a water-soluble derivative of tanshinone IIA, has been proven to possess versatile biological properties, but its pharmacological effect on tracheal smooth muscle remains elusive. This paper presents a study on the relaxant effect and underlying mechanisms of sodium tanshinone IIA sulphonate on mouse tracheal smooth muscle. The relaxant effect of sodium tanshinone IIA sulphonate was evaluated in mouse tracheal rings using a mechanical recording system. Intracellular Ca2+ concentration was measured in primary cultured tracheal smooth muscle cells using confocal imaging system. The results showed that sodium tanshinone IIA sulphonate induced dose-dependent relaxation of mouse tracheal rings in a β-adrenoceptor- and epithelium-independent manner. Pretreatment with the ATP-sensitive K+ channel blocker glibenclamide partly attenuated the relaxation response. Administration of sodium tanshinone IIA sulphonate notably inhibited the extracellular Ca2+-induced contraction. High KCl or carbachol-evoked elevation in the intracellular Ca2+ concentration was also abrogated by sodium tanshinone IIA sulphonate in tracheal smooth muscle cells. In conclusion, the tracheal relaxant effect of sodium tanshinone IIA sulphonate was independent of β-adrenoceptor and airway epithelium, mediated primarily by inhibition of extracellular Ca2+ influx via L-type voltage-dependent Ca2+ channels and partially by activation of the ATP-sensitive K+ channel. These results indicate the potential therapeutic value of sodium tanshinone IIA sulphonate for asthma treatment.
研究领域:
药理学
·
呼吸系统研究
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产品关联
DOI
PubMed
高影响因子
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
·
2018
·Vol. 47
·期 4
·1546-1555
·
IF 5.5
Xu Hao,Zhao Ping,Zhang Wen-Jing,Qiu Jun-Ying,Tan Li,Liu Xiao-Cao,Wang Qian,Luo Xi,She Yu-Shan,Zang Dun-An,Liu Bei-Bei,Cao Lei,Zhao Xiao-Xue,Chen Yuan-Yuan,Li Meng-Yue,Shen Jinhua,Peng Yong-Bo,Xue Lu,Yu Meng-Fei,Chen Weiwei,Ma Li-Qun,Qin Gangjian,Liu Qing-Hua
Background/Aims: Tetraethylammonium chloride (TEA) induces oscillatory contractions in mouse airway smooth muscle (ASM); however, the generation and maintenance of oscillatory contractions and their role in ASM are unclear. Methods: In this study, oscillations of ASM contraction and intracellular Ca2+ were measured using force measuring and Ca2+ imaging technique, respectively. TEA, nifedipine, niflumic acid, acetylcholine chloride, lithium chloride, KB-R7943, ouabain, 2-Aminoethoxydiphenyl borate, thapsigargin, tetrodotoxin, and ryanodine were used to assess the mechanism of oscillatory contractions. Results: TEA induced depolarization, resulting in activation of L-type voltage-dependent Ca2+ channels (LVDCCs) and voltage-dependent Na+ (VNa) channels. The former mediated Ca2+ influx to trigger a contraction and the latter mediated Na+ entry to enhance the contraction via activating LVDCCs. Meanwhile, increased Ca2+-activated Cl- channels, inducing depolarization that resulted in contraction through LVDCCs. In addition, the contraction was enhanced by intracellular Ca2+ release from Ca2+ stores mediated by inositol (1,4,5)-trisphosphate receptors (IP3Rs). These pathways together produce the contractile phase of the oscillatory contractions. Furthermore, the increased Ca2+ activated the Na+-Ca2+ exchanger (NCX), which transferred Ca2+ out of and Na+ into the cells. The former induced relaxation and the latter activated Na+/K+-ATPase that induced hypopolarization to inactivate LVDCCs causing further relaxation. This can also explain the relaxant phase of the oscillatory contractions. Moreover, the depolarization induced by VNa channels and NCX might be greater than the hypopolarization caused by Na+/K+-ATPase alone, inducing LVDCC activation and resulting in further contraction. Conclusions: These data indicate that the TEA-induced oscillatory contractions were cooperatively produced by LVDCCs, VNa channels, Ca2+-activated Cl- channels, NCX, Na+/K+ ATPase, IP3Rs-mediated Ca2+ release, and extracellular Ca2+
研究领域:
生理学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 21
·7417
·
IF 4.6
Wei Yang,Wenping Wang,Song Cai,Peng Li,Die Zhang,Jinhua Ning,Jin Ke,Anguo Hou,Linyun Chen,Yunshu Ma,Wenbin Jin
Malignant cardiac arrhythmias with high morbidity and mortality have posed a significant threat to our human health. Scutellarein, a metabolite of Scutellarin which is isolated fromScutellaria altissimaL., presents excellent therapeutic effects on cardiovascular diseases and could further be metabolized into methylated forms. A series of 22 new scutellarein derivatives with hydroxyl-substitution based on the scutellarin metabolite in vivo was designed, synthesized via the conjugation of the scutellarein scaffold with pharmacophores of FDA-approved antiarrhythmic medications and evaluated for their antiarrhythmic activity through the analyzation of the rat number of arrhythmia recovery, corresponding to the recovery time and maintenance time in the rat model of barium chloride-induced arrhythmia, as well as the cumulative dosage of aconitine required to induce VP, VT, VF and CA in the rat model of aconitine-induced arrhythmia. All designed compounds could shorten the time of the arrhythmia continuum induced by barium chloride, indicating that 4′-hydroxy substituents of scutellarein had rapid-onset antiarrhythmic effects. In addition, nearly all of the compounds could normalize the HR, RR, QRS, QT and QTc interval, as well as the P/T waves’ amplitude. The most promising compound10eshowed the best antiarrhythmic activity with long-term efficacy and extremely low cytotoxicity, better than the positive control scutellarein. This result was also approved by the computational docking simulation. Most importantly, patch clamp measurements on Nav1.5 and Cav1.2 channels indicated that compound10ewas able to reduce the INa and ICa in a concentration-dependent manner and left-shifted the inactivation curve of Nav1.5. Taken together, all compounds were considered to be antiarrhythmic. Compound10eeven showed no proarrhythmic effect and could be classified as Ib Vaughan Williams antiarrhythmic agents. What is more, compound10edid not block the hERG potassium channel which highly associated with cardiotoxicity.Keywords:antiarrhythmic;scutellarein derivatives;Nav1.5 channel blockers;Cav1.2 channel blockers;electrophysiology
研究领域:
药理学
·
心脏病学
·
药物化学
✓ 已验证
DOI
PubMed
高影响因子
Cell Reports Methods
·
2024
·
IF 4.3
Jinli Geng,Yaxiong Yang,Boying Li,Zhen Yu,Shuang Qiu,Wen Zhang,Shixin Gao,Nan Liu,Yi Liu,Bo Wang,Yubo Fan,Chengfen Xing,Xiaodong Liu
Genetically encoded inhibitors of Ca V 1 channels that operate via C-terminus-mediated inhibition (CMI) have been actively pursued. Here, we advance the design of CMI peptides by proposing a membrane-anchoring tag that is sufficient to link the inhibitory modules to the target channel as well as chemical and optogenetic modes of system control. We designed and implemented the constitutive and inducible CMI modules with appropriate dynamic ranges for the short and long variants of Ca V 1.3, both naturally occurring in neurons. Upon optical (near-infrared-responsive nanoparticles) and/or chemical (rapamycin) induction of FRB/FKBP binding, the designed peptides translocated onto the membrane via FRB-Ras, where the physical linkage requirement for CMI could be satisfied. The peptides robustly produced acute, potent, and specific inhibitions on both recombinant and neuronal Ca V 1 activities, including Ca 2+ influx-neuritogenesis coupling. Validated through opto-chemogenetic induction, this prototype demonstrates Ca 2+ channel modulation via membrane-assisted molecular linkage, promising broad applicability to diverse membrane proteins.
研究领域:
神经科学
·
分子生物学
·
生物工程
✓ 已验证
DOI
PubMed
高影响因子
PLANT AND SOIL
·
2013
·Vol. 370
·期 1
·345-354
·
IF 2.638
Wen Bei,Li Longfei,Liu Yu,Zhang Hongna,Hu Xiaoyu,Shan Xiao-quan,Zhang Shuzhen
Background and aims Perfluorinated compounds (PFCs) are of particular environmental concern. The migration of PFCs from soil to plants is a likely pathway for PFCs to enter the human food chain. This study aimed to investigate the uptake mechanisms of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) by maize ( Zea mays L. cv. TY2). Methods Hydroponic greenhouse experiments were performed. Results The kinetics of PFOS and PFOA uptake fitted Mechaelis-Menten equation well, suggesting their carrier-mediated influx processes. Uptake of PFOS was insensitive to metabolic inhibitors (NaN 3 and Na 3 VO 4 ). In contrast, treated with NaN 3 and Na 3 VO 4 reduced the uptake of PFOA by 83 and 43 % respectively. PFOS uptake was decreased by 31 % and 25 % when plants were treated with aquaporin inhibitors, AgNO 3 and glycerol, respectively, while aquaporin inhibitors had no effect on PFOA uptake. Anion channel blockers, 4, 4′-diisothiocyanostibene-2,2′-disolfonate (DID) and 5-nitro 2-(3-phenylpropylamine) benzoic acid (NPPB) inhibited the uptake of PFOS by 33 % and 30 %, respectively. Anion channel blocker anthracene-9-carboxylic acid (9-AC) decreased the uptake of PFOA by 28 %. No competitive uptake was found between PFOS and PFOA. Conclusions Uptake of PFOS and PFOA by maize may have different mechanisms.
研究领域:
环境科学
·
植物生理学
✓ 已验证
DOI
高影响因子
Frontiers in Pharmacology
·
2021
·
IF 5.811
Liu Tao,Cui Lijuan,Xue Huan,Yang Xiaohua,Liu Mengmeng,Zhi Linping,Yang Huanhuan,Liu Zhihong,Zhang Min,Guo Qing,He Peifeng,Liu Yunfeng,Zhang Yi
Angiotensin II type 1 (AT1) receptor blockers (ARBs), as antihypertensive drugs, have drawn attention for their benefits to individuals with diabetes and prediabetes. However, the direct effects of ARBs on insulin secretion remain unclear. In this study, we aimed to investigate the insulinotropic effect of ARBs and the underlying electrophysiological mechanism. We found that only telmisartan among the three ARBs (telmisartan, valsartan, and irbesartan) exhibited an insulin secretagogue role in rat islets. Independent of AT1 receptor and peroxisome proliferator-activated receptor γ (PPARγ), telmisartan exerted effects on ion channels including voltage-dependent potassium (Kv) channels and L-type voltage-gated calcium channels (VGCCs) to promote extracellular Ca2+ influx, thereby potentiating insulin secretion in a glucose-dependent manner. Furthermore, we identified that telmisartan directly inhibited Kv2.1 channel on a Chinese hamster ovary cell line with Kv2.1 channel overexpression. Acute exposure of db/db mice to a telmisartan dose equivalent to therapeutic doses in humans resulted in lower blood glucose and increased plasma insulin concentration in OGTT. We further observed the telmisartan-induced insulinotropic and electrophysiological effects on pathological pancreatic islets or β-cells isolated from db/db mice. Collectively, our results establish an important insulinotropic function of telmisartan distinct from other ARBs in the treatment of diabetes.
研究领域:
药理学
·
内分泌学
·
糖尿病
·
电生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）