ADVANCED FUNCTIONAL MATERIALS
·
2018
·Vol. 28
·期 10
·1705699
·
IF 13.325
Dongyue Su,Xiaoman Liu,Lina Liu,Lei Wang,Hui Xie,Hao Zhang,Xianghe Meng,Xin Huang
Creating an intelligent artificial cell wall to endow fabricated cells with more advanced functionalities is highly desirable. Here, an efficient and cytocompatible approach to generate a type of viable artificial cell wall based on direct self-assembly of coacervate microdroplets on individual cellular surface is developed, which can protect the fabricated cell against various external stresses and can then prolong the storage of the fabricated cells over two months. Moreover, as a type of viable artificial cell wall, it can not only help the fabricated cell sequester nutrients or functionalized substances such as catalase, or iron oxide nanoparticles actively from the solution which then makes the fabricated cell more versatile but also allows the fabricated cell proliferate. Significantly, the artificial cell wall also shows heritable behavior that can reserve its protection to as far as the third-generation daughter cell. Undoubtedly, such an illustrated artificial cell wall provides a conceptually new and promising technique toward the cell-based research as well as application of next generation.
研究领域:
合成生物学
·
细胞工程
·
材料科学
✓ 已验证
DOI
高影响因子
Small
·
2023
·
IF 13.3
Kai Zhang,Cuiping Xu,Xingjian Zhang,Zhiyi Huang,Qixiang Pian,Kunhong Che,Xiaokun Cui,Yueru Hu,Yimin Xuan
The chemical inertness of CO 2 molecules makes their adsorption and activation on a catalyst surface one of the key challenges in recycling CO 2 into chemical fuels. However, the traditional template synthesis and chemical modification strategies used to tackle this problem face severe structural collapse and modifier deactivation issues during the often-needed post-processing procedure. Herein, a CO 2 self-selective hydrothermal growth strategy is proposed for the synthesis of CeO 2 octahedral nanocrystals that participate in strong physicochemical interactions with CO 2 molecules. The intense affinity for CO 2 molecules persists during successive high-temperature treatments required for Ni deposition. This demonstrates the excellent structural heredity of the CO 2 self-selective CeO 2 nanocrystals, which leads to an outstanding photothermal CH 4 productivity exceeding 9 mmol h -1 m cat -2 and an impressive selectivity of >99%. The excellent performance is correlated with the abundant oxygen vacancies and hydroxyl species on the CeO 2 surface, which create many frustrated Lewis-pair active sites, and the strong interaction between Ni and CeO 2 that promotes the dissociation of H 2 molecules and the spillover of H atoms, thereby greatly benefitting the photothermal CO 2 methanation reaction. This self-selective hydrothermal growth strategy represents a new pathway for the development of effective catalysts for targeted chemical reactions.
研究领域:
材料科学
·
催化化学
·
能源化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2019
·Vol. 9
·期 1
·1-17
·
IF 4.011
Ge Xuan,Hu Qiaodan,Lu Wenquan,Cao Sheng,Yang Liang,Xu Mingqin,Xia Mingxu,Li Jianguo
The nucleation pathway plays an important role in vitrification, preparation of glass-ceramic composites and synthesis of metastable materials. In this paper, we studied the nucleation pathway of a novel ferroelectric BaTi 2 O 5 (BT2) during crystallization from undercooled liquid by aerodynamic levitation (ADL) containerless processing and structural analysis. An interesting polymorphic transition of BT2 regulated by the undercooling was observed during the crystallization process: the ferroelectric monoclinic phase ( γ -BT2) was fabricated at low undercoolings and the paraelectric orthorhombic metastable phase ( β -BT2) was obtained from hypercooled liquid. This polymorphic transition phenomenon corresponds to a non-classical nucleation pathway: metastable β -BT2 preferentially nucleates from undercooled melt and γ -BT2 is generated from β phase by solid-state phase transition. The two-step nucleation pathway stems from the structural heredity between the undercooled liquid and crystals. A stronger structural homology exists between the undercooled melt and β -BT2 than γ -BT2 based on diffraction data and atomic configurations analysis. This structural homology coupled with nucleation barrier calculation was used to elucidate the non-classical nucleation pathway of BT2 crystallization: the similarity of the structural unit (Ti-O polyhedra) between the undercooled liquid and the metastable β -BT2 reduces the nucleation barrier and contributes to the preferential precipitation of β -like clusters. This work reveals the formation route of BT2 from cooling melt, which not only benefits the synthesis and application of this novel functional material but also provides a guideline of the crystallization process of titanates from melt at atomic level.
研究领域:
材料科学
·
晶体学
·
凝聚态物理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Yali Yang,Jie Jiang,Jing He,Yun Ju,Yanxiao Jiang,Hong Zhang
Inherited metabolic diseases (IMDs) have drawn considerable attention due to their disruption of normal metabolic pathways. However, conventional extraction techniques are predominantly offline and time-consuming, making them inadequate for the rapid screening required in clinical settings. We developed a novel method involving the extraction of dried sample spots, which are subsequently mixed with charged water (H2O) before infusion electrospray ionization-mass spectrometry analysis. This method was successfully applied for the simultaneous determination of nine amino acid and seven acylcarnitine indicators in dried serum, blood, and urine spots, covering the majority of IMDs routinely screened in hospitals. Compared with conventional approaches, the sensitivity of the new technique improved by nearly threefold, and in some cases, by up to an order of magnitude. The method’s clinical applicability was further confirmed through comparison with the recommended liquid chromatography-tandem mass spectrometry platform and was evaluated using serum samples from three healthy individuals. Additionally, the effect of charged H2O was investigated in detail. A potential mechanism was proposed, suggesting that positively charged H2O enhances ionization by repelling inorganic salts and increasing the density of charges around the target analytes. Although the high surface tension of H2O can, to some extent, suppress ionization, the incorporation of charged H2O in this workflow boosted analytical sensitivity. Overall, this study introduces a simple, rapid, and highly efficient method for the clinical screening of amino acid and acylcarnitine-related IMDs, offering a promising alternative to conventional techniques.
研究领域:
临床化学
·
质谱技术
·
代谢紊乱疾病
·
分析化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Xun Mao,Ting-E Du,Yiyun Wang,Lili Meng
We report here for the first time by using dry-reagent cotton thread-based point-of-care diagnosis devices for low-cost, sensitive and rapid detection of a lung cancer related biomarker, squamous cell carcinoma antigen (SCCA) and a human genetic disease, hereditary tyrosinemia type I related DNA sequences . A model system comprising SCCA as an analyte and a pair of monoclonal antibodies is used to demonstrate the proof-of-concept on the dry-reagent cotton thread based immunoassay device. An enhancement protocol was employed by using two kinds of gold nanoparticle labels for SCCA test which greatly improved the sensitivity of the device. The assay avoids the multiple incubation and washing steps performed in most conventional protein analyses, which is similar with the lateral flow strip technology. Under optimal conditions, the thread based immunoassay device was capable of measuring 1 ng/mL SCCA in 20 min which meet the requirement for clinical diagnosis. DNA detection was successfully realized by using a novel adenosine based molecular beacon probe as reporter probes in the cotton thread based device, the linear range is 75–3000 fmol which is suitable for quantitative test.
研究领域:
诊断学
·
分子生物学
·
纳米技术
·
临床医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2014
·
IF 4.517
Ting-E. Du,Yiyun Wang,Yi Zhang,Tian Zhang,Xun Mao
We used cotton thread as substrate to develop a novel room temperature DNA detection device for low-cost, sensitive and rapid detection of a human genetic disease, hereditary tyrosinemia type I related DNA sequences. A novel adenosine based molecular beacon (ABMB) probe modified on gold nanoparticle was used as reporter probe. In the presence of coralyne, a small molecule which can react with adenosines, the ABMB would form a hairpin structure just like traditional molecular beacon used extensively. In the presence of target DNA sequences, the hairpin structure of ABMB modified on gold nanoparticles will be opened and the biotin group modified at one end of the DNA probes will be released and react with the streptavidin immobilized on the test zone of the cotton thread. The response of the thread based DNA test device is linear over the range of 2.5–100 nM complementary DNA. The ability of our developed device for discriminating the single base mismatched DNA related to a human genetic disease, hereditary tyrosinemia type I, was improved comparing with previous report. It is worth mentioning that the whole assay procedure for DNA test is performed under room temperature which simplified the assay procedures greatly.
研究领域:
分子生物学
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Science China-Life Sciences
·
2024
·
IF 8
Wang Rui,Bao Feixiang,Lu Manjiao,Jia Xiaoyun,Xiao Jiahui,Wu Yi,Zhang Qingjiong,Liu Xingguo
Leber’s hereditary optic neuropathy (LHON) is a debilitating mitochondrial disease associated with mutations in mitochondrial DNA (mtDNA). Unfortunately, the available treatment options for LHON patients are limited due to challenges in mitochondrial replacement. In our study, we reprogramming LHON urine cells into induced pluripotent stem cells (iPSCs) and differentiating them into neural progenitor cells (NPCs) and neurons for disease modeling. Our research revealed that LHON neurons exhibited significantly higher levels of mtDNA mutations and reduced mitochondrial function, confirming the disease phenotype. However, through co-culturing LHON iPSC-derived NPCs with mesenchymal stem cells (MSCs), we observed a remarkable rescue of mutant mtDNA and a significant improvement in mitochondrial metabolic function in LHON neurons. These findings suggest that co-culturing with MSCs can enhance mitochondrial function in LHON NPCs, even after their differentiation into neurons. This discovery holds promise as a potential therapeutic strategy for LHON patients.
研究领域:
神经科学
·
干细胞生物学
·
线粒体遗传学
·
细胞治疗
✓ 已验证
DOI
PubMed
高影响因子
Acta Pharmaceutica Sinica B
·
2024
·
IF 14.7
Yi Wang,Nahui Liu,Lifan Hu,Jingsong Yang,Mengmeng Han,Tianjiao Zhou,Lei Xing,Hulin Jiang
Leber’s hereditary optic neuropathy (LHON) is an ocular mitochondrial disease that involves the impairment of mitochondrial complex I, which is an important contributor to blindness among young adults across the globe. However, the disorder has no available cures, since the approved drug idebenone for LHON in Europe relies on bypassing complex I defects rather than fixing it. Herein, PARKIN mRNA-loaded nanoparticle (mNP)-engineered mitochondria (mNP-Mito) were designed to replace dysfunctional mitochondria with the delivery of exogenous mitochondria, normalizing the function of complex I for treating LHON. The mNP-Mito facilitated the supplementation of healthy mitochondria containing functional complex I via mitochondrial transfer, along with the elimination of dysfunctional mitochondria with impaired complex I via an enhanced PARKIN-mediated mitophagy process. In a mouse model induced with a complex I inhibitor (rotenone, Rot), mNP-Mito enhanced the presence of healthy mitochondria and exhibited a sharp increase in complex I activity (76.5%) compared to the group exposed to Rot damage (29.5%), which greatly promoted the restoration of ATP generation and mitigation of ocular mitochondrial disease-related phenotypes. This study highlights the significance of nanoengineered mitochondria as a promising and feasible tool for the replacement of dysfunctional mitochondria and the repair of mitochondrial function in mitochondrial disease therapies.
研究领域:
眼科学
·
线粒体生物学
·
纳米技术
·
治疗学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）