INTERNATIONAL IMMUNOPHARMACOLOGY
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2025
·
IF 4.7
Xi Zeng,Liefeng Zhang,Jiye He,Yurui Zhang,Hong Lu,Zhuohui Liu,Zhiwei Wang,Zhifeng Wei
Ecliptae Herba is a well-known traditional Chinese medicine, and used for the bleeding and immunity-related diseases in China. This study was aimed to explore the alleviation and mechanisms of eclipta total saponins (ETS) on radiation enteritis (RE). ETS were extracted using the percolation method, and the main active components, Ecl1 and Ecl2, were quantified with HPLC analysis to be 20.39 % and 50.42 %, respectively. Subsequently, a mouse model of RE was established to evaluate the therapeutic effect of ETS and reveal the main active components. The results demonstrated that ETS and Ecl2, but not Ecl1, significantly ameliorated RE in mice, as evidenced by reduced disease activity index scores, shortened colon length, and the down-regulated MPO activity and histopathological injury in colons. Furthermore, ETS and Ecl2 also reversed the increased intestinal permeability and crypt damage. In vitro, Ecl2 obviously inhibited ionizing radiation (IR)-induced apoptosis in HUVECs. This effect was associated with a down-regulated Bax/Bcl2 ratio, release of Cyt-C, and subsequent inhibition of the Caspase-3/PARP cascade. Molecular docking results revealed that Ecl2 potentially binds to the anti-apoptotic proteins PPARγ, but not Bcl2. Additionally, Ecl2 was found to increase the nuclear translocation of PPARγ and the transcription of its downstream target genes CAV1 , aP2 , and CD36. GW9662 abolished Ecl2-mediated anti-apoptosis effects in HUVECs. Collectively, ETS possesses the ability to ameliorate RE, and Ecl2 may act as the main effector component. Ecl2 regulates the Bax/Bcl2-Caspase-3/PARP cascade to inhibit IR-induced apoptosis of endothelial cells by activating PPARγ.
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TALANTA
·
2022
·
IF 6.556
Hao-Fei Sun,Yuan-Yuan Cui,Chang-Qing Zhen,Cheng-Xiong Yang
Microporous organic networks (MONs) are promising in high performance liquid chromatography (HPLC) with large specific surface area, good hydrophobicity and stability. However, their superhydrophobic structures restrict MONs-based HPLC only in reversed-phase mode. To decrease the hydrophobicity of pristine MONs and to expand their broad application in HPLC, here we described the monomer-mediated fabrication of core-shell MON-2COOH@SiO 2 microspheres for reversed-phase liquid chromatography (RPLC)/hydrophilic interaction liquid chromatography (HILIC) mixed-mode chromatography for the first time. The –COOH groups were introduced into MONs’ skeleton to improve their hydrophilicity and to provide hydrophilic interaction sites. The MON-2COOH was grafted onto silica via a monomer mediated method to produce monodispersed core-shell microspheres. By adjusting the concentration of reactants, the thickness of MON-2COOH shell was easily manipulated. The packed MON-2COOH@SiO 2 column showed high resolution and selectivity for separating both hydrophobic (alkylbenzenes, polycyclic aromatic hydrocarbons , anilines and phenols) and hydrophilic (nucleoside and nucleic bases) probes, highlighting the promise of MONs in mixed-mode HPLC. The MON-2COOH@SiO 2 column also achieved good separation to sulfonamides, nonsteroidal anti-inflammatory drugs, flavonoids and phenylurea herbicides , and offered better resolution than commercial C18 and pristine SiO 2 column. Multiple retention mechanisms were also found on MON-2COOH@SiO 2 packed column, underlining the great potential of MONs in mixed-mode HPLC.
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JOURNAL OF CHROMATOGRAPHY A
·
2021
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IF 4.759
Ruijuan Duan,Meiling Qi
This work presents the investigation of using the amphiphilic triblock copolymer composed of poly(ethylene oxide)(PEO)-poly(propylene oxide) (PPO)-poly(ethylene oxide) (PEO) (denoted as EPE) as the stationary phase for gas chromatographic (GC) analyses . The EPE capillary column exhibited moderate polarity and column efficiency of 4348 plates/m determined by naphthalene at 120 °C ( k = 11.52) . Different from the PEG and polysiloxane homopolymers, it showed high-resolution performance towards a wide range of aliphatic and aromatic isomers in terms of polarity and acid-base properties. Particularly, the EPE column displayed distinct advantages for separating the critical isomers of alkanes, anilines and phenols and the components of the lemon essential oil over the commercial PEG and polysiloxane columns. In addition, the EPE column exhibited excellent separation repeatability and reproducibility with the relative standard deviation (RSD) values in the range of 0.03% – 0.08% for run-to-run, 0.14% – 0.61% for day-to-day and 3.1% – 4.0% for column-to-column, respectively. Moreover, the EPE column was investigated in terms of thermal stability, the minimum allowable operating temperature (MiAOT) and sample loadability. Its application to GC-MS analysis of the essential oil demonstrated its feasibility for practical analyses. This work demonstrates the promising future of triblock copolymers as a new class of selective stationary phases for GC analyses, which is barely reported up to date. The findings of this work is of important value for fundamental researches and practical applications.
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JOURNAL OF CHROMATOGRAPHY A
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2021
·
IF 4.759
Changjun Hu,Zhenkun Mao,Zhentao Li,Qiaoyan Li,Zilin Chen
Hydrophilic column combined with mobile phase containing high content of water is a green method for the separation of polar compounds, but there are few related studies, and the separation efficiency and performance of existing columns still needs to be improved. In this work, a novel monolithic column for separation of hydrophilic compounds under both high water content and HILIC condition, was prepared by in-situ polymerization using 4-vinylbenzoic acid (VBA) and 1-(Acryloyloxy)-3-(methacryloyloxy)-2-propanol (AMAP) as functional monomers. The poly(VBA- co -AMAP) monolithic column showed good separation performance towards various polar compounds under different chromatographic conditions based on the π-interaction, hydrophobic and hydrogen bonding interactions provided by 4-vinylbenzoic acid functional monomer. The highest column efficiency for adenine was over 2.15 × 10 5 plates m −1 (theoretical plate, N). In addition, the monolith showed good stability and reproducibility, the relative standard deviations (RSDs) of retention times within days (n = 5), between days (n = 5), between columns (n = 3) and between batches (n = 3) were 0.47-1.13%, 1.20-2.68%, 0.59-1.78% and 1.54-3.60%, respectively. This novel type of monolith has great application potential in the separation of hydrophilic compounds.
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Environmental Technology & Innovation
·
2020
·
IF 3.356
Chao Zhu,Zarak Mahmood,Abdul Saboor Arshad,Jia Liu,Hongrui Ma
Wastewater treatment has become a major global issue due of undegradable pollutants. As toxic water pollutants , namely o-toluidine and p-toluidine are difficult to treat, this study uses the silicon rubber technology to treat them. A pair of modules having single tabular nonporous silicon rubber tube and HCl stripping solution were set up to extract o-toluidine and p-toluidine from high-saline wastewater. Reaction quotient ( Q C ) was calculated after every 12 h intervals to evaluate the equilibrium reaction. The effect of concentration, pH, temperature, ionic strength, and flow rate on the permeability (P) and removal efficiency ( η ) were investigated to illustrate the process parameters and mechanisms of recovery of both elements from wastewater. The results showed that the recovery efficiency was more than 96% with concentration of 2–10g/L. When pH of the extraction solution was 2 or less, the pollutant permeability efficiency was nearly 95%. Moreover, when the salting strength was over 10%, the recovery efficiency increased quickly to some extent. Permeability, which mainly influenced by membrane resistance and desorption rate, initially increased and then decreased when the water flow increased from 2 and 20 mL/min. Aging of silicon rubber tube was also studied with respect to salinity , temperature, and acidity. Acidity and temperature was found to have a significant effect on the tube, permeability increased with an increase in aging, and aging had a minor effect on the elastic properties of the silicon rubber tube. Absorption and diffusion of water in silicon rubber tubes decreased when salinity increased more than 10%, which increased separation of p-toluidine and water. Due to this, there is no need of desalination before membrane extraction in treatment of p-toluidine wastewater with high saline. Thus, this study demonstrates that membrane extraction is a highly energy-efficient process for the effective recovery of o-toluidine and p-toluidine from wastewater with high salinity.
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ANALYTICA CHIMICA ACTA
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2010
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IF 3.757
Mingming Liu,Xin Zhou,Yingyi Chen,Hanlan Liu,Xionghan Feng,Guohong Qiu,Fan Liu,Zhaorui Zeng
In this work, two allyl-functionalised ionic liquids (ILs), 1-allyl-3-methylimidazolium hexafluorophosphate and 1-allyl-3-methylimidazolium bis(trifluoromethanesulphonyl)imide, were used as selective coating materials to prepare chemically bonded ILs-based organic–inorganic hybrid solid phase microextraction fibres. These fibres were prepared with the aid of γ-methacryloxypropyltrimethoxysilane as bridge using sol–gel method and free radical cross-linking technology . The underlying mechanisms of the sol–gel reaction were proposed, and the successful binding of these functional ILs to the sol–gel substrate was confirmed by Fourier transform infrared spectroscopy . These IL-based sol–gel coatings had porous surface structure, high thermal stability , a wide range of pH stability, strong solvent resistance and good coating preparation reproducibility. They also had high selectivity and sensitivity towards strong polar phenolic environmental estrogens (PEEs) and aromatic amines due to the strong electrostatic interactions , hydrogen bonding and π–π interactions provided by the special molecular structure of these imidazolium ILs. Moreover, their characteristics were somewhat different depending on the type of anions in the IL structure. The practical applicability of these IL-based sol–gel coatings was evaluated through the analysis of PEEs in two real water samples. The detection limits were quite low, varying from 0.0030 to 0.1248 μg L −1 . The linearity was very good in the range of 0.1 to 1000 μg L −1 for most analytes, and the relative standard deviation values were below 6%. The relative recoveries were between 83.1 and 104.1% for lake water and between 89.1 and 97.1% for sewage drainage outlet water.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 10
·2209-2221
·
IF 3.286
Zhang Yinchenxi,Duan Yixiang
A solid-phase microextraction (SPME) fiber based on a new polymeric ionic liquid was prepared for the extraction of trace aromatic amines in water and their detection by gas chromatography–mass spectrometry (GC–MS). The newly designed polymeric ionic liquid with two functional groups (benzene ring and ether group) was synthesized and fixed on stainless steel wire to effectively extract aromatic amines. Parameters that affect the extraction efficiency of the SPME fiber (extraction temperature, extraction time, alkali concentration, and salt concentration) were optimized to establish a headspace SPME–GC–MS method. The correlation coefficients were 0.996 or greater for concentration of the aromatic amines ranging from 0.01 to 10 μg mL -1 . In addition, the limits of detection for the new fiber are as low as 0.67 ng mL -1 , which is lower than that obtained with polyacrylate. The relative standard deviations of five consecutive extractions for the solution spiked at 1 μg mL -1 by the same fiber were all below 8.3%, and the interfiber relative standard deviations for the solution spiked at the same concentration ranged from 8.9% to 15.2%. Furthermore, long lifetime and good solvent resistance are exhibited by the fiber. Finally, satisfactory relative recovery in the range from 85.3% to 101.9 % was achieved for two environmental water samples.
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PLoS One
·
2018
·Vol. 13
·期 4
·e0194010
·
IF 2.766
Jin Zhao,Haixiong Xia,Tingyu Yu,Lu Jin,Xuehua Li,Yinghui Zhang,Liping Shu,Lingwen Zeng,Zhixu He
Vanillin (4-hydroxy-3-methoxybenzaldehyde), a food additive with rich milk flavor, is commonly used in the food, beverage and cosmetic industries. However, excessive consumption of vanillin may cause liver and kidney damage. Therefore, methods for detecting and controlling the level of vanillin in food, especially in infant powder, have important practical significance. In this study, we established a colorimetric assay for vanillin detection. The detection was performed under high-temperature and acidic conditions, which can induce the reaction of the aldehyde group of vanillin with the amino group of o-toluidine. The resulting product had a maximum absorption at 363 nm, which was quantified by a UV spectrophotometer. This assay had a limit of detection ( LOD ) of 1 pg mL −1 and a linear range between 1 μg mL −1 and 100 μg mL −1 . The average recoveries at three spiked levels were in the range from 91.1% to 101.6% with a relative standard deviation ( RSD ) of 4.62% ~ 7.27%.
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JOURNAL OF CHROMATOGRAPHY A
·
2018
·
IF 3.716
Rong Wang,Zhenkun Mao,Zilin Chen
A novel monolithic column based on the copolymerization of ethylene dimethacrylate and a deep eutectic solvent (DES), which composed of chlorocholine chloride and itaconic acid, was developed for capillary electrochromatography (CEC). The structure and morphology of the obtained monolithic column was characterized by Fourier transform infrared spectra and scanning electron microscopy, which showed a porous monolithic structure with good permeability. The influence of pH value and acetonitrile content on electroosmotic flow (EOF) of DES-based monolithic column was investigated. The DES-based monolithic column was used as the separation column of CEC and exhibited excellent performance for the separation of neutral compounds, phenols, toluidines, nucleosides, nucleotide bases and alkaloids. The retention mechanism might be attributed to synergistic effect of hydrophobic interaction, hydrogen bond interaction and electronic interaction. The DES-based monolithic column showed good repeatability with relative standard deviations of migration time less than 0.80%, 2.15%, 4.26% and 3.08% for intra-day, inter-day, column-to-column and batch-to-batch, respectively. Thus, organic polymer monolithic columns with DESs as functional monomers are a promising option in chromatographic separation.
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-6
·
IF 3.9
Li Wenfang,Lu Yuze,Wang Jiajun,Li Chuanbi
In this study, a novel artificial enzyme Zn(II)-SMM with simple organic structure for PNPA-directed hydrolysis is proposed. The introduction of hydrophobic quinoline and dipyridinium in Zn(II)-SMM system demonstrates high activity for the complexation of Zn 2+ and hydrolysis of PNPA, and the catalytic rate (k cat /k uncat ) exceeds 5239 times that of non-catalytic systems. The newly designed small molecule organic complex effectively binds to and catalyzes the hydrolysis of substrate PNPA in accordance with Michaelis-Menten kinetics. The mechanism with activation by single Lewis acid provides fundamental insights for the development of small molecule hydrolases.
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ACS Sustainable Chemistry & Engineering
·
2017
·
IF 5.951
Lujiang Xu,Qian Yao,Ying Zhang,Yao Fu
Due to the irregular polymeric structure and carbon based inactive property, lignin valorization is very difficult. In this study we proposed a new route for lignin valorization by which aromatic amines can be directly produced from lignin by ex situ catalytic fast pyrolysis with ammonia over zeolite catalysts. Meanwhile, the obtained pyrolytic biochar can be activated to produce high surface area N-doped carbon for electrochemical application. Wheat straw lignin served as feed to optimize the pyrolysis conditions. MCM-41, β-zeolite, HZSM-5, HY, ZnO/HZSM-5, and ZnO/HY were screened, and ZnO/HZSM-5 (2 wt % Zn, Si/Al = 50) showed the optimal reactivity for producing aromatic amines due to the desired pore structure and acidity. Temperature, residence time, and ammonia content in the carrier gas displayed significant effects on the product distribution. The maximum yield of aromatic amines was obtained at moderate temperatures around 600 °C, 0.57 s, and 75% ammonia in the carrier gas. Under the optimized conditions, the total carbon yields of pyrolytic bio-oil and aromatic amines were 9.8% and 5.6%, respectively. The selectivity of aniline in the aromatic amines was up to 87.3%. Moreover, the pyrolysis byproduct, biochar, was further activated by KOH at 800 °C under ammonia atmosphere for producing N-doped carbon with high surface area. The pyrolytic biochar and N-doped carbon were characterized by elemental analysis, SEM, XRD, nitrogen adsorption–desorption, and XPS. Cyclic voltammetry (CV) and galvanostatic charge–discharge were employed to investigate the electrochemical performance of pyrolytic biochar and N-doped carbon. The specific capacitance of N-doped carbon reached about 128.4 F g–1.
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JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
·
2016
·Vol. 80
·期 2
·567-578
·
IF 1.473
Sui Wenjie,Zhao Wenjie,Zhang Xing,Peng Shusen,Zeng Zhixiang,Xue Qunji
In this paper, we tried to fabricate a compact and thick mercapto functional silica sol–gel coating to achieve excellent corrosion resistance performance on copper. A detailed comparative study of alkylthiol self-assembled monolayers (SAMs) and mercapto functional silica sol–gel coating prepared by 1-octanethiol, n -dodecanethiol and (3-mercaptopropyl)trimethoxysilane (MPTMS) was carried out. The elements composition and chemical bonding of the protective coatings were characterized by X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. In addition, the cross section morphologies of Cu–MPTMS sol–gel coating were investigated by field emission scanning electron microscopy. The wettability of various coatings was investigated by contact angle measurement instrument. Finally, the anti-corrosion ability of the coatings was characterized using an electrochemical workstation. Results demonstrated that compared to other alkylthiol SAMs, the Cu–MPTMS sol–gel coating showed the best anti-corrosion property in sodium chloride solution because it owned a multiple hierarchic Si–O–Si network structure, thus to achieve a higher range of thickness and lengthen the diffusion path way of corrosive medium. It was also found that compact and thick mercapto functional silica sol–gel coating shows better corrosion resistance performance than other SAMs of the same type.
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