FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Yue Pan,Lu Liu,Songfan Tian,Xiaodong Li,Muhammad Hussain,Chunmei Li,Lihong Zhang,Qiumei Zhang,Youbin Leng,Shilong Jiang,Shuyan Liang
In this study, we analyzed and compared the fat globule interfacial compositions and structures in human milk and three types of infant formulas (IF1: supplied with MFGM , IF2: without MFGM and soy lecithin , IF3: supplied with soy lecithin). The results suggested that the interfacial protein composition of IF1 was comparatively closer to human milk, but still lacked certain bioactive MFGM proteins including XO, ADPH and PAS6/7. Considering the interfacial phospholipid , we observed 23, 31, and 29 phospholipid species that could be used to distinguish human milk and infant formulas (IF1, IF2 and IF3). We also found that phosphatidylinositol (PI) and phosphatidylserine (PS), which can absorb lipase, phosphatidylethanolamine (PE), and phosphatidylcholine (PC) and connect functional fatty acids, were lacking in infant formulas. Moreover, the infant formulas had a smaller average particle size of 0.38 μm and a thicker interfacial layer that interacted with the casein micelles. Even if IF1 included MFGM, fat globules structures like those found in human milk did not form, and the majority of the MFGM was still in the aqueous phase in free form. Overall, the comprehensive analysis in this study could be used to simulate or mimic human milk lipids at the supramolecular level.
研究领域:
营养学
·
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Bochen Wei,Fengxia Zhang,Mengyu Wang,Shaobo Li,Muhammad Qamer Abbas,Yuping Wan,Zhenxia Du
To reduce the differences in phospholipids (PLs) of human milk and infant formula, PL-rich raw materials and finished milk powder from the infant formula production line were collected and the phospholipids profiles of raw materials were systematically analyzed by LC-Q-TOF based on phospholipid omics. 108 phospholipids were detected in infant formula, 73 of which were common to human milk, the content of the different phospholipid molecules varied. For the production of raw materials, whey protein concentrate and arachidonic acid powder have the most similar structure to human milk. By varying the ratio of each ingredient, formula can be made closer to human milk. The particle swarm optimization (PSO) algorithm was applied for the optimization of the ratio of raw materials to closely mimic the human milk. Based on the optimized ratio, the optimized formulas were prepared. PCA analysis proved that the optimized formulas were highly effective in mimicking human milk.
研究领域:
营养学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Wei Wei,Jie Yang,Dan Yang,Xiangyu Wang,Zhenyu Yang,Qingzhe Jin,Manyi Wang,Jianqiang Lai,Xingguo Wang
We compared phospholipids (PLs), PL fatty acid (FA) composition, and milk fat globule size and structure in human milk (n = 120) from mothers of full-term and preterm infants during lactation (colostrum, transition, 1 mo, 2 mo, and 3 mo) and 8 brands of infant formulas. The absolute quantification of PLs was analyzed using 31P NMR spectroscopy. Sphingomyelin was the dominant PLs (35.01 ± 3.31%) in human milk, whereas phosphatidylcholine and phosphatidylethanolamine were the dominant PLs in infant formulas. The PL content in preterm milk increased during lactation, whereas that in term milk remained stable. Saturated FAs (mainly 16:0 and 18:0) were the most abundant (>60%) PL FA in both preterm and term milk and increased throughout lactation. The mean diameter of milk fat globules in infant formulas was much smaller than that found in human milk (200 nm vs 5.63 μm). Significant differences were observed between human milk and infant formulas with regard to PLs, suggesting that more research is needed to mimic the PL profile in infant formulas.
研究领域:
营养学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Yan Liu,Junying Zhao,Weicang Qiao,Baoyu Yang,Qian Liu,Yan Liu,Lijun Chen
The phospholipid (PL) composition and milk fat globule (MFG) structure are important for MFG membrane function. However, the effects of various processing methods on these factors remain unclear. In the present study, we compared the effects of different processing methods for infant formula (IF) production on PLs and MFGs. Homogenization and heat sterilization significantly decreased the number of PL species in raw milk (RM, 104). Heat sterilization and spray drying significantly reduced PL content in RM (160.3 ± 12.2 mg/L; p < 0.05). During processing, the average volume diameter (5.67 ± 0.81 μm) and zeta potential (−7.37 ± 0.27 mV) of RM were significantly reduced ( p < 0.05). After homogenization, PLs were distributed in a circular and dotted manner around the MFGs. After spray drying, PL fluorescent rings lost structural integrity. Therefore, the tested processing methods substantially damaged the PL composition and MFG structure. These results may provide a scientific basis for future IF development.
研究领域:
食品科学
·
营养学
✓ 已验证
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2018
·
IF 3.52
Yong Chen,Yifan Zhang,Xiaohou Wang,Jianqun Ling,Guanghua He,Lirong Shen
Cronobacter sakazakii ( Cs ) is a typical foodborne bacterium that infect powdered infant formula (PIF) worldwide. In this study, a recombinant antimicrobial peptide, branded as Funme peptide (FP)was applied to protect PIF from Cs contamination. The result from the antimicrobial activity assay showed that the minimum inhibitory concentration (MIC) of BMAP-27 peptide, FP and Ampicillin against Cs were 250.0, 125.0 and 15.6 μg/mL, respectively, indicating FP possessed higher MIC than that of Ampicillin, and lower MIC than that of BMAP-27. The minimum biofilm eradication concentration (MBEC) assay showed that FP at 2 × MIC (250.0 μg/mL) could completely eradicated Cs biofilms. The antibacterial activity of FP might be due to the increasing permeability and the release of cytoplasmic β-galactosidase of Cs . The results acquired from transmission electron microscopy and scanning electron microscopy indicated that FP induced the disruption and dysfunction of cell walls and membranes. Moreover, safety assay showed that FP had low cytotoxicity to human erythrocytes. The present study investigated the antibacterial effects and mechanisms of FP against Cs , providing promising evidence to apply this novel antimicrobial agent against Cs contamination in foods and food processing facilities.
研究领域:
微生物学
·
食品安全
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2024
·
IF 2.6
Lu Wen,Chen Jichao,Tang Jiongya,chen Yutian,Ma Yingying,Sang Wuhan,Feng Sicheng,Yang Shilong,Wang Yanqin,Li Xu
Zinc is an essential trace element and its deficiency has been related to skin conditions, Alzheimer’s disease, and some types of cancer. Therefore, detecting zinc ions in the human body with high sensitivity is important. Here, two “turn-on” quinoline-based fluorescent probes ( E )-2-((2-(quinolin-2-yl) hydrazono) methyl) phenol ( QSP-H ) and ( E )-4-chloro-2-((2-(quinolin-2-yl) hydrazono) methyl) phenol ( QSP-Cl ) were fabricated for the detection of Zn 2+ . Both the QSP-H and QSP-Cl revealed low LOD (71 nM for QSP-H , 67 nM for QSP-Cl ) and high selectivity, and worked across a broad pH range (3 ‒12 for QSP-H , 3 ‒11 for QSP-Cl ). The HRMS, 1 H NMR titration, DFT calculations and Job’s plot analysis were employed to explore the mechanism of Zn 2+ detection through QSP-H and QSP-Cl . QSP-H and QSP-Cl were effectively applied to the quantitative assessment of Zn 2+ in two infant formula samples and to the bioimaging-based detection of exogenous Zn 2+ in living cells.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yue Sun,Xueying Zhang,Jiayu Li,Xiaodong Li,Lu Liu,Kouadio Jean Eric-Parfait Kouame
We hypothesized that improving the fat globule structure of infant formulae based on the milk fat globule membrane (MFGM) would regulate metabolites and metabolic pathways, making it more similar to the metabolic properties of human milk. Therefore, we prepared infant formulae with different fat globule structures, including two model infant formulae (F1: fat globules surrounded by MFGM; F2: fat globules surrounded by protein) and one commercial infant formulae containing MFGM, and compared their metabolic differences with those of human milk. The number of differential metabolites between each sample and human milk reached 60 (F1), 132 (F2) and 126 (IF1). Glycerophosphocholines were screened as potential biomarkers to distinguish infant formulae from human milk. Compared with F2 and IF1, the enrichment of amino acid metabolism and lipid metabolism pathways was not significant between F1 and human milk. These results emphasized that F1 had the highest similarity to human milk in metabolic properties.
研究领域:
营养学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Yue Sun,Shuaiyi Ma,Yibo Liu,Zhibin Jia,Xiaodong Li,Lu Liu,Qian Ma,Kouadio Jean Eric-parfait Kouame,Chunmei Li,Youbin Leng,Shilong Jiang
The interfacial phospholipid and protein compositions of fat globule in human milk and three different infant formulas (F1: supplied with MFGM and OPO; F2: supplied with OPO and without MFGM; F3: supplied with MFGM and without OPO) were analyzed and compared, and the changes of interfacial composition and structure on their in-vitro infant gastrointestinal digestion were evaluated. The study from the interfacial properties of fat globule to illustrating the effect of interfacial composition and structure on lipolysis and proteolysis. The results showed that, both F1 and human milk had a similar interfacial phospholipid composition and higher sphingomyelin (SM) content. The interfacial protein composition of F1 was closer to human milk. F1 had the average particle size of 3.67 ± 0.12 μm and a phospholipid layer on the surface of triglyceride. Meanwhile, the relatively complete phospholipid ring structure in F1 could always be observed during gastric digestion, while was gradually destroyed during intestinal digestion. Moreover, F2 and F3 are significantly different from HM and F1 in composition and structure of fat globule. Although MFGM is added to commercial infant formula (F3), most MFGM still existed in the aqueous phase of emulsion in free form. Notable, F1 had similar in-vitro lipolysis and proteolysis properties to human milk. These results indicated that the simulation of human milk fat globule at the molecular level and structure feature was the key contributing to digestion behavior.
研究领域:
营养学
·
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Pengcheng Xie,Jialiang Liu,Zonggao Liao,Qi Zhou,Jiajiu Sun,Zheyi Liu,Huihuang Xiong,Hao Wan
Breast milk (BM) fulfills the nutritional needs of infants and sets the standard for infant formula (IF). However, profiling the differential phosphoproteome between BM and IF remains unclear. Herein, a titanium (IV) (Ti 4+ )-immobilized magnetic nanoplatform (Fe 3 O 4 @GO@PDA-Ti 4+ ) was constructed by self-assembly polymerization of dopamine on magnetic graphene oxide, followed by immobilizing Ti 4+ through chelation for phosphopeptide enrichment. Fe 3 O 4 @GO@PDA-Ti 4+ possessed outstanding selectivity (1/1000, a molar ratio of β-casein digests to bovine serum albumin digests) and favorable sensitivity (2.5 fmol/μL), along with rapid magnetic separation. Excellent phosphopeptide capture efficiencies were obtained for BM and IF using Fe 3 O 4 @GO@PDA-Ti 4+ as an adsorbent coupled with liquid chromatography-mass spectrometry/mass spectrometry. There were 191 and 239 phosphopeptides found in BM and IF, respectively, with 36 phosphoproteins identified in both. However, BM and IF shared only 17 phosphopeptides and 4 phosphoproteins. The variation in the phosphoproteome between BM and IF provides valuable insights into the optimization of IF humanization.
研究领域:
生物化学
·
营养学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Longyu Wan,Wen Tu,Jiaxin Zhang,Jiayue Yang,Xu Wang,Jian He,Chaoxin Man,Qianyu Zhao,Feng Zhao,Yujun Jiang
Goat's milk-based infant formula (YIF) has the advantages of high nutritional value and hypoallergenicity, and fewer studies have been conducted on YIF. Therefore, this study examined the changes in physicochemical and functional properties of cow's milk-based infant formula (ZIF) and YIF during 6 months of storage at 25 °C, 37 °C, and 50 °C, respectively. The results showed that YIF had higher pH (7.26), wettability, protein oxidation, good wettability, and lower lactose crystallinity and lipid oxidation compared to ZIF. Further analysis revealed that the higher pH significantly accelerated the rate of the Maillard Reaction (MR), resulting in significantly higher browning of YIF than ZIF, and this difference was statistically significant ( p < 0.05). This is one of the reasons for the decrease in solubility of YIF. This paper explores the differences in storage stability of ZIF and YIF and provides theoretical guidance for the selection of milk-based ingredients.
研究领域:
食品科学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Ling-Zhi Cheong,Chenyu Jiang,Xiaoqian He,Shuang Song,Oi-Ming Lai
Dairy technologists has attempted to produce “improved” infant formulas mimicking human milk by supplementation with bovine MFGM and/or phospholipids-enriched materials. The present study investigated and compared the lipid profile and particle sizes of mature human milk and infant formula fat globules (IF 1, IF 2, IF 3, and IF 4) and elucidated the relationship between physicochemical properties and in vitro simulated gastrointestinal lipolysis rate of the different milk samples. Despite having larger micron-sized fat globules, mature human milk demonstrated the highest gastrointestinal lipolysis rate with higher release of medium- and long-chain saturated fatty acids. In comparison, IF 3, which contained the lowest phospholipids content, demonstrated the lowest gastrointestinal lipolysis rate. Higher gastrointestinal lipolysis rate of mature human milk fat as compared to infant formula fats might be due to the presence of MFGM interfacial layer (phospholipids) surrounding the fat droplets which govern lipase activity on lipid droplets.
研究领域:
营养学
·
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2012
·Vol. 137
·期 22
·5382-5386
·
IF 4.23
Wei Chen,Hao-Hua Deng,Lei Hong,Zeng-Qiang Wu,Sheng Wang,Ai-Lin Liu,Xin-Hua Lin,Xing-Hua Xia
In this study, we demonstrate a more sensitive colorimetric assay for the detection of melamine based on the strong attraction between exocyclic amine groups of melamine and surface-bound AuCl4−/AuCl2− ions on bare gold nanoparticles prepared by the borohydride reduction method. This method is rather simple without further modification of gold nanoparticles and complicated pretreatment of samples. The detection limit of the method is 2.0 × 10−7 g L−1 for melamine in infant formula, which is much lower (about 1000-fold) than that of using citrate-capped GNPs as a colorimetric probe. The proposed scheme could be a good alternative means for on-site and real-time screening of melamine adulterant in infant formula without costly instruments.
研究领域:
分析化学
·
食品安全检测
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）