RSC Advances
·
2021
·Vol. 11
·期 32
·19541-19550
·
IF 3.361
Fangfang Zhu,Honglong Liu,Wenqing Zhang,Chuanyuan Du,Hao Zhu,Xiaodi Du,Xiaosong Hu,Yawei Lin
Biogenic amines (BAs) are organic nitrogenous compounds that are responsible for several biological events. If their concentration reaches the threshold level, it can cause mild to serious health problems in human. A novel bis-styrylphenyl Meldrum's acid derivative (BSMAD) was synthesized and served as a fluorescent turn-on pre-column derivatizing reagent for the quantitative analysis of BAs. A method for the determination of BA by high-performance liquid chromatography (HPLC) was established, which has a low detection limit (0.4 nmol L−1), excellent linearity (R2 ≥ 0.9946) and repeatability (RSD ≤ 3.7% intra-day, RSD ≤ 5.8% inter-day). The proposed method was successfully applied for the determination of BAs in several alcoholic beverages, including yellow wine, red wine, cooking wine, and beer. Satisfactory recoveries were obtained in the range of 94.6–100.5%. Compared with other methods, this pre-column derivatization method using BSMAD is simple, reliable, highly sensitive, and of low interference, providing an effective method for future studies of BAs in different matrices.
研究领域:
分析化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Matter
·
2024
·
IF 17.3
Pingping Fan,Kui Li,Tian Li,Panke Zhang,Shuo Huang
Alcoholic beverages, such as wine, beer, and distilled spirits, are widely produced and consumed in different nations. Different types of alcoholic beverages contain different combinations of flavor compounds. However, rapid and simultaneous analysis of a large variety of compounds in alcoholic beverages by a miniatured and portable device remains a challenge. In this paper, a Mycobacterium smegmatis porin A (MspA) nanopore modified with a phenylboronic acid (PBA) adapter is applied for rapid analysis of a variety of alcoholic beverages. By utilizing custom machine learning algorithms, various cis -diols are identified simultaneously in both distilled and fermented alcoholic beverages, generating unique barcodes for each sample type. Nanopore analysis of alcoholic beverages has also never been carried out previously. Rapid grading of wine sweetness and detection of additives, including sucrose and D-tartaric acid, are also demonstrated, showcasing the significance of this technique in the administration of wine production.
研究领域:
生物技术
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食品科学
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产品关联
DOI
高影响因子
FEMS MICROBIOLOGY LETTERS
·
2021
·Vol. 368
·期 15
·
IF 2.742
Wu Liang,Wen Yongdi,Chen Wenying,Yan Tongshuai,Tian Xiaofei,Zhou Shishui
The reduced yields of acetaldehyde and fusel alcohols through fermentation by Saccharomyces cerevisiae is of significance for the improvement of the flavor and health of alcoholic beverages. In this study, the ADH2 (encode alcohol dehydrogenase) and THI3 (encode decarboxylase) genes of the industrial diploid strain S. cerevisiae XF1 were deleted. Results showed that single-gene-deletion mutants by separate gene deletion of ADH2 or THI3 led to a reduced production of the acetaldehyde or fusel alcohols, respectively. In the meantime, the double-gene-deletion mutant S. cerevisiae XF1-AT was constructed by deleting the ADH2 and THI3 simultaneously. An equivalent level of the ethanol production by the S. cerevisiae XF1-AT could be achieved but with the yields of acetaldehyde, isoamyl alcohol and iso-butanol reduced by 42.09%, 15.65% and 20.16%, respectively. In addition, there was no interaction between the ADH2 deletion and THI3 deletion in reducing the production of acetaldehyde and fusel alcohols. The engineered S. cerevisiae XF1-AT provided a new strategy to alcoholic beverages brewing industry for reducing the production of acetaldehyde as well as the fusel alcohols.
研究领域:
微生物学
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发酵技术
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食品科学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 21
·3450
·
IF 5.561
Yisong Liu,Chunfeng Liu,Xin Xu,Chengtuo Niu,Jinjing Wang,Feiyun Zheng,Qi Li
Acetaldehyde is regarded as an important flavor compound in alcoholic beverages. With the advantages of rapidity, low cost and high sensitivity, fluorescent probe could be used as a new tool for the detection of acetaldehyde. Here, an effective fluorescence sensing method based on fluorescent probe N1 (FPN1) was established in this study. The function of FPN1 relies on the nucleophile substitution reaction and photoinduced electron transfer (PET), resulting in a fluorescence increase. Remarkably, the pretreatment background removal method (BRM) was successfully applied for removal of the interference of pyruvate and acetal. The linearity range (LR), limit of detection (LOD) and recovery of the fluorescence sensing method with BRM were 0.0053–200 mg/L, 0.0016 mg/L and 94.02–108.12%, respectively, which showed a broader detection range and better performance on sensitivity compared with the traditional quantitation using gas chromatography (GC). Furthermore, successful application of the method in real samples indicated the advantages of low-cost and rapidity for small-scale detection while assuring the accuracy, which provides a new strategy for the detection of acetaldehyde concentration in alcoholic beverages.Keywords:acetaldehyde;fluorescence detection;fluorescent probe;alcoholic beverages;photoinduced electron transfer
研究领域:
分析化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 20
·3316
·
IF 4.7
Xiaonian Cao,Yunfei Hou,Qingqing Liu,Qian Yang,Min Liu,Haixu Lin,Qingxi Ren,Jian Mao
The unique flavour contribution of higher alcohols in alcoholic beverages has received growing attention; however, there is a dearth of information on their in vivo metabolic kinetics. In this study, the composition and content of higher alcohols in different alcoholic beverages from ChineseBaijiuandLujiuwere studied via in vivo analysis using Bama pigs to elucidate the mechanisms for intoxication of alcohol in vitro and in drinkers. Direct injection combined with gas chromatography–mass spectrometry (GC-MS) were used to accurately quantify a total of 14 higher alcohols in five alcoholic beverages. Based on the external standard method, a total content of 289.37–938.33 mg/L was detected, mainly 1-butanol, 3-methyl-1-butanol, 1-hexanol, 2-methyl-1-propanol and 2-butanol. Then, headspace solid-phase microextraction (HS-SPME) and solid-phase extraction (SPE) combined with GC-MS analysis strategy, respectively, were adopted to continuously monitor the changes in the concentrations of ethanol and 11 higher alcohols in the blood within 24 h after gavage of different alcoholic beverages, and the key pharmacokinetic parameters were analysed. The peak concentration (Cmax) and area under curve (AUC) of blood higher alcohols were significantly lower than those of ethanol (p <0.05), accompanied by a later peak time (Tmax) and a larger apparent clearance rate (CL_F), and there were certain differences between the same higher alcohols in different alcoholic beverages and between different higher alcohols in the same alcoholic beverage. This work provides valuable insights into the metabolism of alcoholic beverages.
研究领域:
食品科学
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动物生理学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
Royal Society Open Science
·
2018
·Vol. 5
·期 12
·
IF 2.504
Haiyan Qi,Huacai Chen,Yan Wang,Li Jiang
Ethyl carbamate (EC), a potential carcinogen, can be formed during the fermentation and storage of alcoholic beverages. In this work, quantitative detection of EC in alcoholic beverages by using surface-enhanced Raman spectroscopy (SERS) is reported. Flower-shaped silver nanostructure substrates and silver nanocube substrates were prepared and employed as SERS platform. Flower-like silver substrates had better Raman enhancement effect on EC and were selected for further EC detection. In EC SERS spectra based on flower-shaped silver substrates, the strongest and reproducible characteristic band at 857 cm−1 was chosen for establishing a linear regression model in the concentrations ranging from 10−5 to 10−9 M, which effectively extended the application scope of the quantitative model for determination EC. Furthermore, a real alcoholic beverage was tested to verify the feasibility and reliability of the method.
研究领域:
分析化学
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食品安全
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光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Kaige Zhang,Rong Guo,Yunhe Wang,Jing Wang,Qiujun Nie,Guifen Zhu
Aliphatic aldehydes are a class of organic compounds containing aldehyde groups, which are widespread, and closely related to people's daily life and health. In this work, a series of terpenes based hydrophobic deep eutectic solvents were designed and synthesized using hexafluoroisopropanol as hydrogen bond donor and menthol/thymol as hydrogen bond acceptor. Then they are used as extraction solvent in dispersive liquid-liquid microextraction for extracting and determining seven aliphatic aldehydes from drinking water and alcoholic beverage combined with high performance liquid chromatography-ultraviolet. Due to the fact that these hydrophobic deep eutectic solvents are liquid at the room temperature, a density greater than that of water, a lower viscosity (≤26.10 mPa s, 25 °C), after extraction and centrifugation, the microvolume DES-rich phase in the bottom is convenient for collection and direct analysis without further dissolution or dilution with organic solvents. Some factors affecting the extraction recovery were optimized by one variable-at-a-time and response surface methodology. Under the optimal conditions, the enrichment factors for the seven aliphatic aldehydes were 48–56. The method had good performance: linear ranges of 1.0–200, 0.5–200, 0.2–200, 0.4–400, 1.0–400, 0.4–400 and 0.4–400 μg L −1 for seven aliphatic aldehydes ( r 2 ≥ 0.9949), limits of detection of 0.1–0.5 μg L −1 , intra-day and inter-day precisions <4.9%. The recoveries of seven aliphatic aldehydes ranged from 76.0 to 119.0%. The proposed dispersive liquid-liquid microextraction method is simple, rapid, highly efficient, and green, which effectively reduces the amount of toxic chemical reagents used and their impact on the environment. Rapid and efficient detection of aliphatic aldehydes helps ensure a healthy diet and has great application prospects in food safety analysis.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Nian Shi,Wenxuan Li,Yuwei Liu,Shumei Yan,Xia Xu,Di Chen
The determination of biogenic amines (BAs) in alcoholic beverages is crucial for assessing their health impact, ensuring beverage quality, and guaranteeing safety. Herein, a rapid one-pot derivatization/magnetic solid-phase extraction (OPD/MSPE) method was proposed using 6-aminoquinolinyl-N-hydroxysuccinimide carbamate as the derivatization reagent and magnetic hydroxyl-functionalized multi-walled carbon nanotubes as the extraction material. Integration of derivatization and extraction steps simplifies the sample preparation process, taking only three minutes and eliminating the need for centrifugation by utilizing magnetic sorbent. The resulting desorption solution was directly analyzed by high-performance liquid chromatography-fluorescence detection (HPLC-FLD) without any evaporation or reconstitution steps. The integrated OPD/MSPE-HPLC-FLD method demonstrates excellent linearity (R 2 > 0.992), accuracy (relative recoveries: 85.1–109.2%), precision (RSDs≤9.7%) and detection limits (limits of detection: 0.3–2 ng/mL). It has been successfully applied to determine free BAs in various alcoholic beverages, including red wine, Baijiu, Huangjiu, and beer. This method enables rapid, sensitive and precise analysis of BAs in alcoholic beverages.
研究领域:
分析化学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2015
·Vol. 162
·期 6
·H321
·
IF 3.266
Meiling Wang,Jianwei Zhao
A facile electrochemical sensor was fabricated via highly dispersing Multi-walled carbon nanotubes (MWCNTs) in ionic liquid-graphene oxides (IL-GO) "water" at room temperature. The introduction of IL (1-butyl-3-methylimidazolium hexafluorophosphate) to GO water resulted in the high dispersibility of MWCNTs and also caused fast electron transfer rates of the three dyes of Ponceau 4R (E124), Tartrazine (E102) and Allura red (E129) at the IL-GO-MWCNT composites modified glassy carbon electrode (IL-GO-MWCNT/GCE). Well-separated oxidation peaks and enhanced peak currents of Ponceau 4R, Allura red, and Tartrazine were observed at the IL-GO-MWCNT modified GCE. For individual detection, the linear responses of Ponceau 4R, Allura red, and Tartrazine were in the concentration ranges of 1.0 × 10−9-1.0 × 10−6, 8.0 × 10−10-5.0 × 10−7, and 1.0 × 10−8-1.0 × 10−6 mol L−1 with detection limits of 8.0 × 10−10, 5.0 × 10−10, and 7.0 × 10−9 mol L−1 (S/N = 3), respectively. For simultaneous determination by synchronous change of the concentrations of Ponceau 4R, Allura red, and Tartrazine, the linear response ranges were 8.0 × 10−9-1.5 × 10−7, 5.0 × 10−9-4.5 × 10−7 and 2.0 × 10−8-1.3 × 10−7 mol L−1 with the detection limits of 6.0 × 10−9, 3.0 × 10−9, and 1.0 × 10−8 mol L−1 (S/N = 3), respectively. The fabricated sensors were further applied for the detection of Ponceau 4R, Allura, red and Tartrazine in alcoholic beverages.
研究领域:
分析化学
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食品科学
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产品关联
DOI
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2025
·
IF 3.5
Jun Wang,Qiuyue Liao,Gai Liang,Yuruo Gong,Jiaxu Yao,Li Zhou,Wei Kevin Zhang,Xiaojun Li
Ethosomes are ethanol-based liposomes which may self-assemble in alcoholic beverages and exhibit dual effects: nanoscale ethanol has liver-targeting property with potential hepatotoxicity on the one side, while ethosomes can encapsulate active constituents that provide hepatoprotective benefits on the other side. This study investigated the dual role of ethosomes, focusing on their existence, characterization, potential toxicity, and toxicological mechanisms. Our results confirmed the existence of ethosomes in alcoholic beverages using particle size analysis, ultracentrifugation, liquid chromatography-mass spectrometry, and transmission electron microscopy. Both in vitro and in vivo experiments revealed that ethosomes can cause significant liver injury in a short period, associated with abnormal secretion of transforming growth factor-β ( TGF-β ) by parenchymal cells and subsequent apoptosis of Kupffer cells. Notably, the toxicity of ethosomes was significantly reduced when encapsulated natural active compounds like oleanolic acid. In conclusion, ethosomes could form self-assembly in the production progress of the integrated alcoholic beverages. The present study underscored the dual impact of ethosomes on alcoholic liver disease and offered new insights into the safety risk assessment of alcoholic beverages.
研究领域:
药剂学
·
毒理学
·
纳米技术
·
肝病学
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食品科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Lin Zhu,Xuebo Song,Fei Pan,Tuohetisayipu Tuersuntuoheti,Fuping Zheng,Qing Li,Siqi Hu,Feifei Zhao,Jinyuan Sun,Baoguo Sun
Ferulic acid (FA) and tetramethyl pyrazine (TMP) are intrinsic bioactive compounds in baijiu, and kafirin is the major protein of sorghum, which is the raw material of baijiu. In this study, the interactions of kafirin-FA and kafirin-TMP were investigated by multiple spectroscopic and molecular modeling techniques. Fluorescence spectra showed that intrinsic fluorescence of kafirin drastically quenched because of the formations of kafirin-FA and kafirin-TMP complexes. The CD studies indicated that the combination with FA or TMP decreased the α -helix content of kafirin slightly. The shifts and intensity changes of UV–Vis, FTIR and fluorescence spectra confirmed the formations of complexes. Moreover, the molecular docking and molecular dynamics studies showed that hydrophobic interactions and hydrogen bonds played major roles in the formations of kafirin-FA and kafirin-TMP complexes, and the formations of complexes made kafirin structures more compact. This work is of great importance for further quality improvement in baijiu and alcoholic beverages.
研究领域:
食品科学
·
化学
·
分子生物学
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光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）