JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2011
·Vol. 16
·期 2
·449-456
·
IF 2.234
Han Ruixia,Cui Lin,Ai Shiyun,Yin Huanshun,Liu Xianggang,Qiu Yanyan
A tyrosinase (Tyr) biosensor has been constructed by immobilizing tyrosinase on the surface of Mg–Al–CO 3 hydrotalcite-like compound film (HTLc) modified glassy carbon electrode (GCE) for the determination of polyphenols. The negatively charged tyrosinase was adsorbed firmly on the surface of a positively charged HTLc/GCE by electrostatic interactions and retained its activity to a great degree. The modified electrode was characterized by cyclic voltammetry and AC impedance spectra. Polyphenols were determined by a direct reduction of biocatalytically generated quinone species. The different parameters, including pH, temperature, and enzyme loading were investigated and optimized. Under the optimum conditions, Tyr/HTLc electrode gave a linear response range of 3–300, 0.888–444, and 0.066–396 μM with a detection limit (S/N = 3) of 0.1, 0.05, and 0.003 μM for catechol, caffeic acid, and quercetin, respectively. In addition, the repeatability and stability of the enzyme electrode were estimated. Total polyphenol contents of real samples were also determined to study the potential applicability of the Tyr/HTLc/GCE biosensor.
研究领域:
生物传感器
·
酶学分析
·
电化学检测
✓ 已验证
DOI
高影响因子
Micro & Nano Letters
·
2018
·Vol. 13
·期 10
·1382-1385
·
IF 0.841
Xin Xiao,YiHui Wang,DongEn Zhang,JunYan Gong,Ying Zhang,JuanJuan Ma,Tao Yang,ZhiWei Tong
Highly uniform columnar-shaped CeO 2 was successfully prepared in large quantities by calcining a precursor, which was prepared by a simple hydrothermal method. The structure and morphology of the precursor and the columnar CeO 2 were characterised by X-ray diffraction and scanning electron microscopy. The columnar CeO 2 was fixed on a glassy carbon electrode to obtain a columnar CeO 2 sensor for detection of phenol. The anodic peak current was proportional to the phenol concentration in the range of 0.005–0.225 mmol l −1 ( R = 0.995) and 0.225–1.125 mmol l −1 ( R = 0.998) with the detection limit of 0.9 μmol l −1 . This electrochemical method has the advantages of simplicity and ease of fabrication, rapid detection, low cost of the apparatus and wide dynamic ranges.
研究领域:
材料科学
·
环境科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Zhiyi Yao,Wenqi Ge,Mingwei Guo,Keren Xiao,Yadong Qiao,Zhong Cao,Hai-Chen Wu
We report a simple pyrene-based fluorescent probe , sodium 8-(2,4-dinitrophenoxy)pyrene-1,3,6-trisulfonate (HPTS-DNP), for the ultrasensitive and visual detection of thiophenol in 100% aqueous media. The sensing mechanism of this method is based on nucleophilic aromatic substitution on HPTS-DNP caused by thiophenol to afford a highly fluorescent product, 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS). The extremely low fluorescence background of HPTS-DNP and high quantum yield of HPTS ensure the superior sensing performance toward thiophenol, including rapid responses, off-on detection mode and excellent sensitivity. The detection limit is as low as 0.49 nmol L −1 according to the measurements with a spectrofluorimeter. This probe also features good selectivity (response ratio of thiophenol to interferents >30), precision (2.93%) and accuracy (102%). This approach could be applied for monitoring the thiophenol concentrations in environmental water samples. HPTS-DNP also showed good cell permeation capacity and low cellular cytotoxicity, indicating further application in bioimaging studies.
研究领域:
分析化学
·
环境科学
·
生物医学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Qiang Cao,Yushi Xiao,Na Liu,Rong Huang,Chen Ye,ChiHsien Huang,Huan Liu,Gang Han,Lidong Wu
Metal-organic frameworks (MOFs) have potential as heterogeneous biomimetic catalysts due to their high surface area, tunable porosity, and diversity of metals and functional groups. However, the activity and substrate specificity of pure monomeric MOFs still must be improved. Here, a size-selective MOF coating on the external surface of a biomimetic MOF is used to overcome these limitations of pure monomeric MOFs. The synthesis process of the biomimetic material involved first the coating of porous coordination network (PCN-222) cores with polyvinylpyrrolidone (PVP) as a structure-directing agent and then the modification of zeolitic imidazolate frameworks (ZIF-8). The yolk/shell PCN-222@ZIF-8-based sensor showed high selectivity for catechol but not for dopamine or L-DOPA. The PCN-222@ZIF-8 hybrid material-based sensor displayed 10 times more sensitivity than the PCN-222-based sensor, with low limit of detection (LOD) at 33 nmol L −1 . Thus, ZIF-8 is a shell which allows the diffusion of specific substrate to the active sites of the PCN-222 and enriches the substrate. Furthermore, the PCN-222@ZIF-8 exhibits solvent adaptability, which overcomes the drawback of the natural HRP enzyme. This work opens a new avenue for construction of MOF@MOF biomimetic sensors and other applications.
研究领域:
材料科学
·
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2021
·Vol. 146
·期 13
·4280-4289
·
IF 4.616
Jinyan Du,Shuangqing Qi,Tingting Fan,Ying Yang,Chaofeng Wang,Qin Shu,Shujuan Zhuo,Changqing Zhu
Herein, a simple and facile one-step hydrothermal carbonization synthesis procedure for the fabrication of N, Cu-doped carbon quantum dots (N, Cu-CQDs) as a peroxidase-mimicking enzyme was reported. The peroxidase-like performance of N, Cu-CQDs was assessed based on the oxidative coupling reaction of phenol with 4-aminoantipyrine (4-AAP) in the presence of hydrogen peroxide (H2O2). The N, Cu-CQDs/4-AAP/H2O2 system was applied to sensing phenol based on double signals of absorption spectra (or colorimetric visualization) as well as fluorescence spectra. The obtained limits of detection (LODs) were as low as 0.12 μM and 0.02 μM, respectively. Moreover, the proposed method was successfully applied to the determination of phenol in sewage with satisfactory recovery. Our results demonstrate that the N, Cu-CQDs/4-AAP/H2O2/phenol sensing system has a great potential prospect for applications in environmental chemistry and biotechnology.
研究领域:
环境化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2019
·Vol. 19
·期 7
·4020-4025
·
IF 1.093
Song Bing Bing,Zhen Yi Fan,Yin Hao Yong,Song Xu Chun
In this work, platinum nanoparticles were modified on graphite-like carbon nitride (g-C3N4) surface via a simple one-pot deposition reduction method, and the prepared Pt/g-C3N4 was used as electrode materials for phenol sensing. The morphology
and crystallographic structure of Pt/g-C3N4 were characterized by Scanning transmission electron microscope (STEM) and X-ray diffraction (XRD). The electrochemical performance of Pt/g-C3N4 toward phenol was investigated in details and the results
showed that the Pt/g-C3N4 sensor had favorable sensing ability for phenol detection. Under the optimum parameters, the electrochemical sensor had a wide range of 2 to 20 µM, lower detection limits of 0.667 µM (S/N═3), and higher sensitivity
of 1.21084 mA µM−1 in phenol detection, indicating the excellent capability of the newly developed sensor to determine phenol. Moreover, the design of the Pt/g-C3N4 sensor may provide a potential material for actual application in phenol
detection due to its merits of simplicity, reliability, sensitivity and low-cost.
研究领域:
材料科学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Yueyue Qian,Chuang Ma,Shupeng Zhang,Juanjuan Gao,Maoxiang Liu,Kangjun Xie,Shuang Wang,Kuan Sun,Haiou Song
A highly conductive and electrochemically active film has been successfully fabricated by combining beta-cyclodextrin with acid-treated poly(3, 4-ethylenedioxythiophene):polystyrene (denoted as CD-f-PEDOT:PSS). The unique properties of the high performance CD-f-PEDOT: PSS sensor was investigated by utilizing X-ray photoelectron spectroscopy, atomic force microscope, cyclic voltammetry and amperometric i-t , etc. The CD-f-PEDOT:PSS film exhibits high-sensitivity towards dopamine (DA) and catechol (CT) without employing expensive noble metal or indium tin oxide (ITO) substrate and consuming much more time to polish the surface of traditional electrodes. The detection limits (S/N = 3) for DA and CT in phosphate buffer solutions (PBS, pH = 7.4) are 9.596 nM and 0.0275 μM, respectively. Furthermore, the CD-f-PEDOT: PSS film sensor exhibits good anti-interference and reproducibility. The encouraging results as well as facile preparation method suggest the CD-f-PEDOT: PSS film sensor is a promising alternative electrode to traditional ones such as ITO, gold or glassy carbon electrodes.
研究领域:
生物传感器
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
·
2023
·Vol. 37
·期 4
·e7027
·
IF 3.9
Rui Wang,Shu-Hui Yin,Bi-Liu Lan,Heng-Huan Ruan,Zhi-Hui Qiu,Zhong Zhang
Two cyclen-based multidentate N,O ligands, 1,4,7,10-tetrazazcyclododecane-N,N′,N″,N″′-tetra- p -methylbenzoic acid (H 4 L 1 ) and 1,4,7,10-tetrazazcyclododecane-N,N′,N″,N″′-tetra- p -cinnamic acid (H 4 L 2 ), were reacted with Zn (II) salts leading to a four-fold interpenetrated 3D binodal PtS-type network {[Zn 2 (HL 1 )Cl]·5H 2 O} n ( Zn-1) along with a 1D ribbon-like polymeric chain {[Zn 3 (HL 2 )Cl 3 (H 2 O) 2 ]·2CH 3 COCH 3 ·3H 2 O} n ( Zn-2 ). Both two compounds exhibited excellent water stability in a wide pH range from 2 to 12 and emitted either UV or visible blue-purple light. They can behave as recyclable fluorescent probes for highly selective sensing of nitrophenols with low detection limits. Additionally, the blue-purple emission of Zn-2 can be efficiently quenched by PA with a concentration as low as 20 μM; thus, the naked-eye detection of PA can be fulfilled under a 365 nm UV lamp. Based on the DFT calculation and spectral analysis evidences, it can be proposed that the selective detection of nitrophenols by two Zn (II) compounds is owing to a synergistic effect of different quenching mechanisms along with the interactions between probes and analytes.
研究领域:
化学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Yuanyuan Wang,Weimin Guan,Yulin Yang,Huiling Lan,Yu Wang,Yun Wang,Juan Han,Lei Wang
Immobilization of enzymes in porous organic framework (POF) materials is popular strategy to stabilize enzymes. For such solid enzyme catalysis system, improving the catalytic efficiency is challenging due to the diffusion resistance from solid-liquid interface and inner pores. Here, UCST-pH dual responsive polymeric carrier (PEG- b -PAAm- b -P(GMA- co -AAc)) was synthesized to immobilize cytochrome c (Cyt c), which impart the reversibly insoluble-soluble property to the immobilized Cyt c. The PEG- b -PAAm- b -P(GMA- co -AAc) could serve as an insoluble-soluble matrix to fast and efficiently immobilize Cyt c via covalent attachment, achieving a remarkable 92 % loading efficiency within just 120 min. The obtained insoluble PEG- b -PAAm- b -P(GMA- co -AAc)-Cyt c micelles exhibited an improvement in thermal, pH stability and reusability. The completely soluble PEG- b -PAAm- b -P(GMA- co -AAc)-Cyt c conjugates accelerated substrate diffusion and then enhanced the catalytic efficiency. These excellent advantages led to low detection limit (1.99 μM), lower than the presently reported biosensors based on enzyme mimics in the colorimetric detection of phenol. This UCST-pH dual responsive window presents a new platform to efficiently control the immobilization and release of enzymes, which will achieve excellent stability and catalytic efficiency.
研究领域:
生物化学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2016
·
IF 2.76
Guohui Li,Kaiyue Sun,Dawei Li,Pengfei Lv,Qingqing Wang,Fenglin Huang,Qufu Wei
Bacterial cellulose-gold nanoparticles (BC-AuNPs) hybrid nanofibers were synthesized by in-situ chemical reduction. The obtained BC-AuNPs nanofibers were characterized by a series of analytical techniques. The results revealed that gold nanoparticles (AuNPs) were successfully deposited on the surfaces of bacterial cellulose nanofibers. Then, the as-prepared BC-AuNPs hybrid nanofibers were attached onto the electrode surface followed by adding a certain amount of laccase (Lac) and Nafion to construct novel a biosensing platform. The prepared electrochemical biosensor was employed to detect hydroquinone. The sensor showed excellent electrocatalysis towards dopamine with low detection limit (5.71 nM), and wide linear range (30–100 nM). Moreover, the biosensor also showed good repeatability, reproducibility, selectivity and stability and was successfully used in the detection of hydroquinone in lake water samples, thus providing a promising method for hydroquinone detection in water environments.
研究领域:
生物技术
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Shuying Li,Zhengang Han,Zhilan Wang,Yanjun Feng,Yubao Lan,Yaqi Zhao,Yuling Gao,Kainan Kang,Peiyao Du,Xiaoquan Lu
For optimal energy transfer in self-luminous lanthanide metal–organic frameworks (Ln-MOFs), the energy of the lowest triplet excited state must align with ideal energy levels. Failure to meet this condition can lead to reverse energy transfer, reducing luminous efficiency. In this study, we developed a mixed-ligand MOF, Eu-TCPP-BOP, which exists as an ECL self-enhancing luminophore. We used SPECM to study the role of boron ligands as a bridge for electron transport in improving the ECL performance of Eu-TCPP. The ligands H4TCPP and 5-BOP act as electron donor and shuttle, facilitating electron transport during the synthesis of Eu-TCPP-BOP and promoting energy transfer to the excited state of the acceptor Ln3+, thus enhancing overall energy transfer in Ln-MOF. The results indicate that the introduction of boron ligands enhances the ECL intensity of Eu-TCPP by a factor of 1.4 under voltage excitation. As an ECL sensing platform, it demonstrates high sensitivity and selectivity for the detection of catechol, with a concentration range of 1∼70 μM and a detection limit of 0.35 μM.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
·
2016
·
IF 3.035
Qianghua Ye,Fanyong Yan,Dechao Shi,Tancheng Zheng,Yinyin Wang,Xuguang Zhou,Li Chen
Nitrogen and boron co-doped carbon dots (BCNDs1–3) were prepared from three kinds of borate via a facile hydrothermal method . The as-prepared BCNDs did not shift with the change of excitation wavelength and possess good water dispersibility, strong fluorescence emission with high fluorescent quantum yield of 29.01%, 51.42%, 68.28%, respectively. Subsequently, these BCNDs were exploited as excellent Hg 2 + ion and 2,4,6-trinitrophenol (TNP) probe. The efficient selective detection of Hg 2 + can be attributed to non-radiative electron/hole recombination annihilation through an effective electron transfer process and the detection of TNP can be attributed to the fluorescence resonance energy transfer process (FRET). The results show that the BCNDs2 is the most sensitive fluorescence probe for Hg 2 + ions and TNP detection as low as Hg 2 + 7.3 nM and TNP 0.35 μM compared with BCNDs1 and BCNDs3. The as-prepared BCNDs possess the advantages of good selectivity, fast response and a broad linear detection. They were applied to sensing and imaging of human umbilical vein endothelial cells , showing low cytotoxicity and good biocompatibility .
研究领域:
材料科学
·
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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