JOURNAL OF APPLIED POLYMER SCIENCE
·
2025
·Vol. 142
·期 14
·e56693
·
IF 2.8
Zi-Ang Xia,Long Wang,Chaojie Li,Xue Li,Jingxue Yang,Baoming Xu,Heng Zhang
The augmentation of chitosan's (CS) antioxidative and antibacterial properties via grafting with phenolic acids constitutes a significant method for expanding the utility of chitosan. In this investigation, four phenolic acids (gallic acid, GA; protocatechuic acid, PCA; hydroxybenzoic acid, HA; and salicylic acid, SA) were conjugated with CS utilizing carbodiimide (EDC)-mediated grafting. The resultant phenolic acid-CS graft copolymers underwent characterization employing infrared spectroscopy, x-ray diffraction, and thermogravimetric analysis. Furthermore, ex vivo antioxidative and antibacterial assays were performed. Findings revealed that among the synthesized phenolic acid-chitosan graft copolymers, gallic acid-chitosan (GA-CS) demonstrated the highest antioxidative and antibacterial efficacy, primarily attributed to its elevated grafting ratio and abundance of hydroxyl groups. The antioxidative and antibacterial performances of the remaining three graft copolymers followed this order: CS-PCA > CS-HA > CS-SA, aligning with the trend in grafting ratios. This correlation arises from the higher grafting ratios of copolymers yielding more active OH groups, thereby exhibiting enhanced antioxidative and antibacterial properties. Phenolic acid-chitosan graft polymers exhibit promising antioxidative and antibacterial attributes, making them suitable additives for application in the food preservation packaging industry.
研究领域:
食品科学
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材料科学
·
微生物学
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DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 18
·10272-10281
·
IF 3.049
Yide Xia,Ying Liu,Nannan Shi,Xungao Zhang
In this article, the catalyst Au/γ-Fe2O3@hydroxyapatite (Au/γ-Fe2O3@HAP) consisting of Au nanoparticles supported on the core–shell structure γ-Fe2O3@HAP was prepared through a deposition–precipitation method. The catalyst was characterized by transmission electron microscopy, X-ray powder diffraction, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, N2 adsorption–desorption and atomic absorption spectrometry. The as-prepared Au/γ-Fe2O3@HAP exhibited excellent performance for the reduction of 4-nitrophenolate (4-NP) to 4-aminophenolate (4-AP) in the presence of NaBH4 at room temperature. Thermodynamic and kinetic data on the reduction of 4-NP to 4-AP catalyzed by the as-prepared catalyst were studied. The as-prepared catalyst could be easily separated by a magnet and recycled 6 times with over 92% conversion of 4-NP to 4-AP. In addition, the as-prepared catalyst showed excellent catalytic performance on other nitrophenolates. The TOF value of this work on the reduction of 4-NP to 4-AP was 241.3 h−1. Au/γ-Fe2O3@HAP might have a promising potential application on the production of 4-AP and its derivatives.
研究领域:
材料科学
·
化学
·
环境科学
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DOI
PubMed
高影响因子
Journal of Advanced Research
·
2020
·
IF 6.992
Xiaolong Hao,Zhongqiang Pu,Gang Cao,Dawei You,Yang Zhou,Changping Deng,Min Shi,Shivraj Hariram Nile,Yao Wang,Wei Zhou,Guoyin Kai
Salvia miltiorrhiza Bunge is an herb rich in bioactive tanshinone and salvianolic acid compounds. It is primarily used as an effective medicine for treating cardiovascular and cerebrovascular diseases. Liposoluble tanshinones and water-soluble phenolic acids are a series of terpenoids and phenolic compounds, respectively. However, the regulation mechanism for the simultaneous promotion of tanshinone and salvianolic acid biosynthesis remains unclear. This study identified a R2R3-MYB subgroup 20 transcription factor (TF), SmMYB98 , which was predominantly expressed in S. miltiorrhiza lateral roots. The accumulation of major bioactive metabolites, tanshinones, and salvianolic acids, was improved in SmMYB98 overexpression (OE) hairy root lines, but reduced in SmMYB98 knockout (KO) lines. The qRT-PCR analysis revealed that the transcriptional expression levels of tanshinone and salvianolic acid biosynthesis genes were upregulated by SmMYB98-OE and downregulated by SmMYB98-KO . Dual-Luciferase (Dual-LUC) assays demonstrated that SmMYB98 significantly activated the transcription of SmGGPPS1 , SmPAL1 , and SmRAS1 . These results suggest that SmMYB98-OE can promote tanshinone and salvianolic acid production. The present findings illustrate the exploitation of R2R3-MYB in terpenoid and phenolic biosynthesis, as well as provide a feasible strategy for improving tanshinone and salvianolic acid contents by MYB proteins in S. miltiorrhiza .
研究领域:
植物生物技术
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生药学
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分子生物学
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转录调控
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2020
·
IF 4.244
Min Shi,Yao Wang,Xiaorong Wang,Changping Deng,Wenzhi Cao,Qiang Hua,Guoyin Kai
Tanshinones and phenolic acids are two major groups of natural bioactive compounds in Salvia miltiorrhiza which exert excellent pharmacological activities. Due to low content in S.miltiorrhiza , it is urgent to enhance the production of the two compounds to meet the increasing need. The Arabidopsis basic helix-loop-helix (bHLH) transcription factor MYC2 was introduced into S. miltiorrhiza hairy roots. In the best transgenic line AtMYC2-3 , tanshinone yield was 14.06 mg/g dw which was increased by 5.45-fold more than that in the control line, while phenolic acid production was enhanced to 95.9 mg/g dw, a 3.3-fold increase compared with the control. Transcript levels of some genes involved in biosynthesis of tanshinones and phenolic acids were noticeably upregulated. Dual-luciferase assay revealed that AtMYC2 activated the expression of biosynthetic genes including SmDXS2 and SmTAT . These results revealed that AtMYC2 plays a positive role in accumulation of both tanshinones and phenolic acids which provides a promising strategy for engineering tanshinone and phenolic acid production in S. miltiorrhiza .
研究领域:
植物学
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分子生物学
·
药用植物学
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产品关联
DOI
高影响因子
CRYSTAL GROWTH & DESIGN
·
2020
·
IF 4.089
Xin Chen,Duanxiu Li,Zongwu Deng,Hailu Zhang
Ketoconazole (KTZ) is an imidazole derivative that is used to treat a wide variety of fungal infections and other human diseases. As a class II compound in the biopharmaceutics classification system, KTZ has low aqueous solubility. To improve this disadvantage, five phenolic acids were employed to form cocrystals with KTZ. The antifungal and anti-inflammatory activity of the phenolic acids may bring an enhanced therapeutic effect and reduce the risk of hepatotoxicity at the same time. The structural information on these five crystalline samples and KTZ were fully disclosed by single-crystal X-ray diffraction. In addition to a previously reported carboxylic acid···imidazole N interaction, hydroxyl···imidazole N hydrogen bonding also appears in the KTZ cocrystals as a main supramolecular heterosynthon. The short strong hydrogen bonds were observed in two cocrystal structures, which contain slightly longer carboxylic O–H length. 15N solid-state NMR also provided valuable clues for the confirmation of the molecular/ionization states. The dissolution tests revealed that all the newly obtained cocrystals exhibit significantly improved solubility compared to KTZ.
研究领域:
药学
·
化学
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DOI
高影响因子
Current Pharmaceutical Analysis
·
2021
·Vol. 17
·期 4
·509-519
·
IF 0.89
Hou Zhuoni,Liang Zongsuo,Li Yuanyuan,Su Feng,Chen Jipeng,Zhang Xiaodan,Yang Dongfeng
Background: Although chromatography and spectrometry-based methods have been used to analyse phenolic acids in Chinese traditional medicine Salvia miltiorrhiza Bunge (SMB), quantitative nuclear magnetic resonance (qNMR) has never previously been used to analyse fresh SMB root extracts.
Objective: To establish a fast and simple method of quantitating danshensu, lithospermic acid, rosmarinic acid, and salvianolic acid B content in fresh SMB root using 1H-NMR spectroscopy. Method: Fresh SMB root was extracted using a 70% methanol aqueous solution and quantitatively
analysed for danshensu, lithospermic acid, rosmarinic acid, and salvianolic acid B using 1H-NMR spectroscopy. Different internal standards were compared and the results were validated using highperformance liquid chromatography. Results: The established method was accurate
and precise with good recovery. The LOD and LOQ indicated the excellent sensitivity of the method. The robustness was testified by the modification of four different parameters, and the differences among each parameter were all less than 2%. Conclusion: qNMR offers a fast, reliable, and accurate
method of identifying and quantifying danshensu, lithospermic acid, rosmarinic acid, and salvianolic acid B in fresh SMB root extracts.
研究领域:
生药学
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分析化学
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中医药
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DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 13
·1839
·
IF 5.561
Qiao Yang,Xiaoyi Zhang,Huini Qin,Feijun Luo,Jiali Ren
Cancer is still the leading cause of death across the world, and there is a lack of efficient therapies.Lactarius hatsudakeis a mushroom with a food and medicine homology that contains numerous biologically active substances. This study aimed to investigate the composition of extracts fromLactarius hatsudake(L. hatsudake) and their anti-cancer function and molecular mechanisms. Our results showed that the total phenolic content ofL. hatsudakeextracts was 139.46 ± 5.42 mg/g. The following six phenolic compounds were identified fromL. hatsudakeextracts by HPLC and UPLC-QTOF/MS: gallic acid, pyrogallol, chlorogenic acid, ferulic acid, myricetin, and cinnamic acid. Colorectal cancer cell HCT116 and hepatic cancer cell HepG2 were used to evaluate the anti-cancer function of theL. hatsudakeextracts. Compared with HepG2 cells, theL. hatsudakeextracts showed stronger anti-cancer activity against HCT116 cells and these were used to study molecular mechanisms. The results indicated that theL. hatsudakeextracts could arrest the cancer cell cycle and inhibit cancer cell proliferation, which may be mediated by the MAPK/NFκB/AP-1 signalling pathway; theL. hatsudakeextracts also promoted cancer cell apoptosis through a mitochondrial-dependent pathway. Taken together, these findings demonstrate thatL. hatsudakeethanol extracts contain six main phenolics and illustrate the remarkable potentiality ofL. hatsudakeas a source of natural phenolics for cancer prevention and as an adjuvant in the treatment of functional foods.Keywords:Lactarius hatsudake;phenolic acids;anti-cancer;molecular mechanism
研究领域:
药用真菌学
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肿瘤生物学
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植物化学
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2017
·
IF 5.651
Xiahui Wang,Yiu Fai Tsang,Yuhao Li,Xiubing Ma,Shouqing Cui,Tian-Ao Zhang,Jiajun Hu,Min-Tian Gao
In this study, it was found that the type of phenolic acids derived from rice straw was the major factor affecting ethanol fermentation by Pichia stipitis . The aim of this study was to investigate the inhibitory effect of phenolic acids on ethanol fermentation with rice straw. Different cellulases produced different ratios of free phenolic acids to soluble conjugated phenolic acids, resulting in different fermentation efficiencies. Free phenolic acids exhibited much higher inhibitory effect than conjugated phenolic acids. The flow cytometry results indicated that the damage to cell membranes was the primary mechanism of inhibition of ethanol fermentation by phenolic acids. The removal of free phenolic acids from the hydrolysates increased ethanol productivity by 2.0-fold, indicating that the free phenolic acids would be the major inhibitors formed during saccharification. The integrated process for ethanol and phenolic acids may constitute a new strategy for the production of low-cost ethanol.
研究领域:
生物化学
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发酵工程
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DOI
PubMed
高影响因子
Waste and Biomass Valorization
·
2020
·Vol. 11
·期 12
·6555-6563
·
IF 2.851
Hou Rongrong,Shi Jie,Ma Xiubing,Wei Huanran,Hu Jiajun,Tsang Yiu Fai,Gao Min-Tian
Abstract Phenolic compounds are widely used in different research fields, such as pesticides, medicines, and food. In this study, phenolic acids (PAs) were extracted from rice straw and were found to exhibit a strong inhibitory effect on Botrytis cinerea . B. cinerea mycelial growth and spore generation decreased by 86.18% and 69.10%, respectively, following 0.2 g/L phenolic acid treatment. Confocal microscopic images demonstrated that phenolic acids changed the morphology of B. cinerea . The addition of phenolic acids to B. cinerea- infected tomato leaves increased PAL (phenylalaninammo-nialyase) and PPO (polyphenol oxidase) activities, and decreased POD (peroxidases) and CAT (catalase) activities in the leaves, indicating that phenolic acids enhanced the tolerance of tomato leaves to B. cinerea by reducing oxidative stress. Chlorophyll fluorescence imaging revealed that phenolic acids could alleviate the destruction of the photosynthetic system of B. cinerea -infected leaves. These results provide new insight into the use of phenolic acids from rice straw, through which a complete green cycle of ecological production can be established. Graphic Abstract
研究领域:
植物病理学
·
农业科学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Yao Chen,Qingfa Tang,Qinglan Zhu,Meiting Lu,Errong Chang,Ziting Gao,Zidong Wang,Lu Zhang,Qiang Liu,Junju Zou,Zhong Zuo,Chunyan Shen,Cuiping Jiang
Atherosclerosis (AS) is a chronic cardiovascular disease characterized by the accumulation of excess lipids within arterial walls. Since oxidative stress is one of the key drivers in its pathogenesis, antioxidative therapy serves as a promising strategy for AS treatment. Despite the potent antioxidant properties of polyphenolic salvianic acid A (SAA) and salvianolic acid B (SAB), clinical application is limited by their short half-life, insufficient target selectivity, and poor chemical stability. To overcome these limitations, we developed a biomimetic platelet membrane-coated polyphenol–cerium dioxide nanozyme complex (SS-CeO2@PLTM) as a broad-spectrum antioxidative system for comprehensive AS treatment. The SS-CeO2@PLTM was constructed by conjugating SAA and SAB on the surface of cerium dioxide via a one-pot method, followed by surface coating with a biomimetic platelet membrane. Systematic characterization using UV–vis spectroscopy, Fourier transform infrared (FT-IR), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy-energy dispersive X-ray spectroscopy (TEM-EDS) elemental mapping analysis, Forster resonance energy transfer (FRET), molecular simulations, and pH-responsive release assays validated the successful synthesis and pH-responsive drug release behavior of SS-CeO2@PLTM. It was also found that SS-CeO2@PLTM exhibited broad-spectrum antioxidant activities, effectively scavenging superoxide anions, DPPH, H2O2, and intracellular reactive oxygen species (ROS) while enhancing hepatic SOD and GSH activities. Further cellular uptake and in vivo targeting assays confirmed their specific targeting for atherosclerotic plaques. SS-CeO2@PLTM demonstrated great efficiency in attenuating oxidative stress, inhibiting lipid uptake and lipid accumulation, mediating cholesterol efflux, and inhibiting inflammatory factor (IL-1β, IL-6, and TNF-α) secretion in RAW264.7 cells. We also found that SS-CeO2@PLTM exhibited improved lipid metabolism and diminished plaque area in apoE–/– mice via attenuating oxidative stress and NF-κB-mediated inflammation. Furthermore, biosafety was verified via hemolysis and in vivo safety tests. This study is the first to reveal the superior efficacy of the PLTM-based nanozyme complex in targeted AS therapy, offering a paradigm shift for multifactorial cardiovascular disease management.
研究领域:
纳米医学
·
心血管疾病
·
药物纳米技术
·
抗氧化治疗
·
仿生学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 15
·2842
·
IF 3.06
Li Duan,Chenmeng Zhang,Chenjing Zhang,Zijing Xue,Yuguang Zheng,Long Guo
TheArtemisia argyileaf (AL) has been used as a traditional medicine and food supplement in China and other Asian countries for hundreds of years. Phytochemical studies disclosed that AL contains various bioactive constituents. Among bioactive constituents, phenolic acids have been recognized as the main active compounds in AL. To the best of our knowledge, no research has been focused on extraction method for the bioactive phenolic acids from AL. Nowadays, deep eutectic solvents (DESs) are emerging as a new type of green and sustainable solvent for efficient extraction of bioactive compounds from natural products. In the present study, an environmentally friendly extraction method based on DESs was established to extract bioactive phenolic acids from ALs. Diverse tailor-made solvents, including binary and ternary DESs, were explored for simultaneous extraction of four phenolic acids (3-caffeoylquinic acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, and 4,5-di-O-caffeoylquinic acid) from AL. The results indicated that the ternary DES composed of a 2:1:2 molar ratio of choline chloride, malic acid, and urea showed enhanced extraction yields for phenolic acids compared with conventional organic solvents and other DESs. Subsequently, the extraction parameters for the four phenolic acids by selected tailor-made DESs, including liquid–solid ratios, water content (%) in the DESs, and extraction time, were optimized using response surface methodology and the optimal extraction conditions were: extraction time, 23.5 min; liquid–solid ratio, 57.5 mL/g (mL of DES/g dry weight of plant material); water content, 54%. The research indicated that DESs were efficient and sustainable green extraction solvents for extraction of bioactive phenolic acids from natural products. Compared to the conventional organic solvents, the DESs have a great potential as possible alternatives to those organic solvents in health-related areas such as food and pharmaceuticals.Keywords:deep eutectic solvents;Artemisia argyileaves;phenolic acids;extraction;response surface methodology
研究领域:
植物化学
·
药学
·
食品科学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Bingjie Zhang,Weiqing Lan,Zhicheng Wang,Zhe Shao,Jing Xie
This study synthesized five phenolic acid-chitosan copolymers utilizing the carbodiimide-mediated chemical crosslinking reaction. Comprehensive evaluations were conducted on their structural attributes, physicochemical properties, and biological activities. Fourier transform infrared confirmed successful grafting of phenolic acids onto chitosan via amide linkages. Additionally, ultraviolet–visible absorption spectroscopy and proton nuclear magnetic resonance analyses revealed novel absorption peaks between 200 and 400 nm and 6.0–8.0 ppm, respectively, attributable to the incorporated phenolic acids. Notably, the chitosan-gentisate acid copolymer exhibited significantly enhanced biological activity ( p < 0.05 ) compared to pure chitosan and the other four conjugates, attributed to its highest grafting degree of approximately 295.93 mg/g. These modified chitosan derivatives effectively preserved the quality of sea bass ( Lateolabrax japonicus ) during refrigerated storage, extending its shelf-life by up to 9 days, 7 days, and 4 days relative to control, chitosan, and gentisate acid groups.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）