JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
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IF 6.1
Ming-Shan Chen,Jia-Xin Wang,Hao Zhang,Jia-Gen Cui,Yi Zhao,Jin-Long Li
Phthalates are widely used to improve the flexibility of poly(vinyl chloride) (PVC) polymer agriculture products. Di(2-ethylhexyl) phthalate (DEHP) is a type of addition to plastic and can lead to many health problems. Hemeoxygenase-1 (HO-1) is an extremely important molecule that releases enzymatic products to promote ferroptosis. This research aimed to explore the function of HO-1 in DEHP-induced renal proximal tubule cell ferroptosis. In the experiment, ICR male mice are exposed to (0, 50, 200, and 500 mg/kg BW/day) DEHP for 28 days. Here, we observed that DEHP induced glomeruli atrophy and the tubules swell. Furthermore, DEHP exposure could increase ferrous iron content and decrease antioxidant activity. We also found that DEHP exposure increased the expression of nuclear factor-erythroid 2 p45-related factor 2 (NFE2L2) in the nucleus. In particular, the expression of (HO-1) is significantly increased both in protein and mRNA levels. Glutathione peroxidase 4 (GPX4) as an endogenous control of ferroptosis was downregulated, which proved the occurrence of ferroptosis. In the study, exposure to DEHP activated the NFE2L2/HO-1 signaling pathway and resulted in ferroptosis of the proximal tubule. This research connects ferroptosis with HO-1, providing new insights into the potential roles of phthalates in nephrotoxicity.
研究领域:
毒理学
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肾脏生理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Redox Biology
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2022
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IF 10.787
Yi Zhao,Hao Zhang,Jia-Gen Cui,Jia-Xin Wang,Ming-Shan Chen,Hao-Ran Wang,Xue-Nan Li,Jin-Long Li
The global rate of human male infertility is rising at an alarming rate owing to environmental and lifestyle changes. Phthalates are the most hazardous chemical additives in plastics and have an apparently negative impact on the function of male reproductive system. Ferroptosis is a recently described form of iron-dependent cell death and has been linked to several diseases. Transferrin receptor (TfRC), a specific ferroptosis marker, is a universal iron importer for all cells using extracellular transferrin. We aim to investigate the potential involvement of ferroptosis during male reproductive toxicity, and provide means for drawing conclusions on the effect of ferroptosis in phthalates-induced male reproductive disease. In this study, we found that di (2-ethylhexyl) phthalate (DEHP) triggered blood-testis barrier (BTB) dysfunction in the mouse testicular tissues. DEHP also induced mitochondrial morphological changes and lipid peroxidation, which are manifestations of ferroptosis. As the primary metabolite of DEHP, mono-2-ethylhexyl phthalate (MEHP) induced ferroptosis by inhibiting glutathione defense network and increasing lipid peroxidation. TfRC knockdown blocked MEHP-induced ferroptosis by decreasing mitochondrial and intracellular levels of Fe 2+ . Our findings indicate that TfRC can regulate Sertoli cell ferroptosis and therefore is a novel therapeutic molecule for reproductive disorders in male patients with infertility.
研究领域:
生殖毒理学
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细胞生物学
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环境健康
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产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
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2025
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IF 26.8
Jiajun He,Yun Huang,Huihui Li,Jin Bao,Heguo Zeng,Zhanpeng Du,Fengliang Wang,Xiaoyan Ma,Xuepeng Zhong,Bo Yu,Xing Li,Mingshan Wang,Yuanhua Lin,Bingshu Guo,Haijun Cao
This study employs molecular engineering to modulate HOMO levels and electronic structures, designing high-performance phthalate-based anodes (ZnPA, CaPA, CuPA) to address challenges like limited capacity and poor cycling in organic lithium-ion batteries. CuPA delivers 749 mAh g −1 after 100 cycles at 0.1 A g −1 and retains 200 mAh g −1 after 2500 cycles at 1 A g −1 . ZnPA achieves 650 mAh g −1 at 0.1 A g −1 and maintains 200 mAh g −1 after 1500 cycles at 1 A g −1 . CaPA shows increased capacity at higher current (197 mAh g −1 at 1 A g −1 ) due to (100) plane expansion. Redox mechanisms differ: CuPA and ZnPA carbonyl group facilitates lithium storage by a reversible enolization reaction, while the activated benzene ring exhibits redox activity for reversible lithiation/delithiation, with Cu 2+ irreversibly reducing to Cu⁺, while Zn 2+ remains stable. CaPA enables efficient lithium storage by the reversible intercalation and deintercalation of lithium ions on its (100) crystallographic plane. Full cells with LiFePO 4 exhibit excellent performance: CuPA||LFP and ZnPA||LFP retain over 50% capacity after 1000 cycles; CaPA||LFP shows outstanding rate capability (103 mAh g −1 at 4C). This work demonstrates that molecular structural engineering is an effective strategy for enhancing organic electrode materials.
研究领域:
材料科学
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电化学
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能量存储
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电池技术
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分子工程
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2023
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IF 11.4
Jing Wu,Jun-Hua Zhou,Dong-Feng Liu,Jie Wu,Ru-Li He,Zhou-Hua Cheng,Hui-Hui Li,Wen-Wei Li
Plastics–microorganism interactions have aroused growing environmental and ecological concerns. However, previous studies concentrated mainly on the direct interactions and paid little attention to the ecotoxicology effects of phthalates (PAEs), a common plastic additive that is continuously released and accumulates in the environment. Here, we provide insights into the impacts of PAEs on the dissemination of antibiotic resistance genes (ARGs) among environmental microorganisms. Dimethyl phthalate (DMP, a model PAE) at environmentally relevant concentrations (2–50 μg/L) significantly boosted the plasmid-mediated conjugation transfer of ARGs among intrageneric, intergeneric, and wastewater microbiota by up to 3.82, 4.96, and 4.77 times, respectively. The experimental and molecular dynamics simulation results unveil a strong interaction between the DMP molecules and phosphatidylcholine bilayer of the cell membrane, which lowers the membrane lipid fluidity and increases the membrane permeability to favor transfer of ARGs. In addition, the increased reactive oxygen species generation and conjugation-associated gene overexpression under DMP stress also contribute to the increased gene transfer. This study provides fundamental knowledge of the PAE–bacteria interactions to broaden our understanding of the environmental and ecological risks of plastics, especially in niches with colonized microbes, and to guide the control of ARG environmental spreading.
研究领域:
环境科学
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微生物学
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生态毒理学
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分子生物学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）