LAB ON A CHIP
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2025
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IF 5.4
Tianhao Xue,JiaNing Shen,Wanting Lin,Jiahui Zhou,Xiaofang Zhang,Jen-Tsai Liu,Ching-Jung Chen,Guixian Zhu
Ketone bodies are key products of fat metabolism, primarily consisting of acetoacetate (AcAc), β-hydroxybutyrate (BHB), and acetone (Acetone). Monitoring the concentration of ketone bodies in sweat can reflect the metabolic status of the body, it is also particularly significant in areas such as diabetes management, exercise monitoring, and the evaluation of the ketogenic diet. This paper presents a microfluidic patch for sweat collection and multiplex detection of AcAc, BHB and glucose. The microfluidic patch can achieve time-sequential sensing through Tesla valves, hydrophilic coatings, and unique chamber structural design. The concentrations of the three substances are quantified using colorimetric methods. Additionally, this study has designed a colorimetric app which can achieve automatic framing and detect the grayscale value of the colored area. Experimental results show that the patch can accurately detect changes in the concentrations of the three substances within specific ranges. The linear detection range for AcAc is 0.25 mM to 8 mM, the limit of detection (LOD) is 0.08 mM; for BHB, linear detection range is 0.05 mM to 0.80 mM, LOD is 0.02 mM; and for glucose, linear detection range is 62.50 μM to 1000 μM, LOD is 20.83 μM. In the future, this technology is expected to be applied to portable metabolic monitoring devices, offering a convenient solution for personal health management
研究领域:
生物医学工程
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代谢监测
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健康科技
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACY AND PHARMACOLOGY
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2024
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IF 2.8
Lou Yutao,Zou Xiaozhou,Pan Zongfu,Huang Zhongjie,Zheng Shuilian,Zheng Xiaowei,Yang Xiuli,Bao Meihua,Zhang Yuan,Gu Jinping,Zhang Yiwen
ObjectivesThis study aimed to reveal the anti-fibrotic effects of Botrychium ternatum (Thunb.) Sw. (BT) against idiopathic pulmonary fibrosis (IPF) and to preliminarily analyze its potential mechanism on bleomycin-induced IPF rats.MethodsThe inhibition of fibrosis progression in vivo was assessed by histopathology combined with biochemical indicators. In addition, the metabolic regulatory mechanism was investigated using 1H-nuclear magnetic resonance-based metabolomics combined with multivariate statistical analysis.Key findingsFirstly, biochemical analysis revealed that BT notably suppressed the expression of hydroxyproline and transforming growth factor-β1 in the pulmonary tissue. Secondly, Masson’s trichrome staining and hematoxylin and eosin showed that BT substantially improved the structure of the damaged lung and significantly inhibited the proliferation of collagen fibers and the deposition of extracellular matrix. Finally, serum metabolomic analysis suggested that BT may exert anti-fibrotic effects by synergistically regulating tyrosine metabolism; phenylalanine, tyrosine and tryptophan biosynthesis; and synthesis and degradation of ketone bodies.ConclusionsOur study not only clarifies the potential anti-fibrotic mechanism of BT against IPF at the metabolic level but also provides a theoretical basis for developing BT as an effective anti-fibrotic agent.
研究领域:
药理学
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代谢组学
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呼吸病学
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2022
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IF 6.633
Licheng Zhang,Jinfeng Li,Ruirui Liu,Yongquan Zhou,Yuze Zhang,Lianmin Ji,Lijuan Li
Efficient separation of lithium from salt lake brine with high Na/Li ratio is of great importance for the development of new energy industry. Liquid-liquid extraction using β-diketone system was proved to be an effective approach to separate lithium from alkaline metal ions, while the separation effect of lithium to alkali earth ions needs further investigation. In this work we proposed an extraction process using HBTA/TRPO system to recovery lithium from salt lake brine with high Na/Li ratio. The conditions of saponification, extraction, eluting, stripping and regeneration process was optimized by equilibrium experiments. Through the process, lithium, calcium and magnesium were extracted to the organic phase and then stripped step by step to obtain lithium-rich solution, then the stability of the process and organic was tested by cycle experiments, the results indicated the proposed method was effective and stable. Theoretical study based on density functional theory was conducted to expound the extraction selectivity to different metal ions, and the energy change, electrostatic potential and Mulliken charge of the complexes was obtained, the calculated results suggested the stability of the complexes followed the sequence: Mg 2+ > Ca 2+ > Li + > Na + > K + , which is consistent with the experimental results. The present work may provide a novel way to efficiently recover lithium from salt lake brine with high Na/Li ratio.
研究领域:
化学工程
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新能源材料
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理论计算
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DOI
高影响因子
ANALYTICAL CHEMISTRY
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2024
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IF 6.7
Qiyu Wang,Qingzhou Liu,Geng Zhong,Tailin Xu,Xueji Zhang
Ketogenic diets have attracted substantial interest in the treatment of chronic diseases, but there are health risks with long-term regimes. Despite the advancements in diagnostic and therapeutic methods in modern medicine, there is a huge gap in personalized health management of this dietary strategy. Hence, we present a wearable microneedle biosensor for real-time ketone and glucose monitoring. The microneedle array possesses excellent mechanical properties, allowing for consistent sampling of interstitial biomarkers while reducing the pain associated with skin puncture. Vertical graphene with outstanding electrical conductivity provides the resulting sensor with a high sensitivity of 234.18 μA mM–1 cm–2 and a low limit detection of 1.21 μM. When this fully integrated biosensor was used in human volunteers, it displayed an attractive analytical capability for tracking the dynamic metabolite levels. Moreover, the results of the on-body evaluation established a significant correlation with commercial blood measurements. Overall, this cost-effective and efficient sensing platform can accelerate the application of a ketogenic diet in personal nutrition and wellness management.
研究领域:
生物医学工程
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营养学
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传感技术
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DOI
PubMed
高影响因子
Nature Communications
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2022
·Vol. 13
·期 1
·1-13
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IF 17.694
Pan An,Sun Xiao-Meng,Huang Feng-Qing,Liu Jin-Feng,Cai Yuan-Yuan,Wu Xin,Alolga Raphael N.,Li Ping,Liu Bao-Lin,Liu Qun,Qi Lian-Wen
Disordered hepatic glucagon response contributes to hyperglycemia in diabetes. The regulators involved in glucagon response are less understood. This work aims to investigate the roles of mitochondrial β-oxidation enzyme HADHA and its downstream ketone bodies in hepatic glucagon response. Here we show that glucagon challenge impairs expression of HADHA. Liver-specific HADHA overexpression reversed hepatic gluconeogenesis in mice, while HADHA knockdown augmented glucagon response. Stable isotope tracing shows that HADHA promotes ketone body production via β-oxidation. The ketone body β-hydroxybutyrate (BHB) but not acetoacetate suppresses gluconeogenesis by selectively inhibiting HDAC7 activity via interaction with Glu543 site to facilitate FOXO1 nuclear exclusion. In HFD-fed mice, HADHA overexpression improved metabolic disorders, and these effects are abrogated by knockdown of BHB-producing enzyme. In conclusion, BHB is responsible for the inhibitory effect of HADHA on hepatic glucagon response, suggesting that HADHA activation or BHB elevation by pharmacological intervention hold promise in treating diabetes.
研究领域:
内分泌学
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代谢学
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分子生物学
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糖尿病研究
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DOI
PubMed
高影响因子
Food Bioscience
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2022
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IF 5.318
Chang Liu,Yahui Guo,He Qian,Yuliang Cheng
Inulin can regulate the intestinal micro-ecological balance, but there are few studies on the changes of intestinal microbiota and its metabolites in vitro and vivo , SCFAs and amino acids in particular. In this study, the human fecal microbiota glycolysis model and a mouse model were used to study the mechanism of inulin on intestinal microecology. Combined with metabolism of microbiome and metabolome , the cross-synergism mechanism of inulin-micribiota-metabolites-metabolism was investigated. The results revealed that after inulin was fermented by human intestinal microbiota , the relative abundance of Lactobacillus , Bifidobacterium , Prevotella , Catenibacterium , and Agathobacter increased. In vivo , the concentration of SCFAs increased after inulin was fermented, and the abundance of Parabacteroides , Muribaculaceae and Alloprevotella increased. Both in vivo and in vitro experiments revealed that inulin can regulate the expression of 5 genes involved in Valine , leucine and isoleucine degradation. In addition, the metabolome results confirmed that Valine, leucine and isoleucine degradation, Tyrosine metabolism, and Synthesis and degradation of ketone bodies are regulatory pathways of inulin. More importantly, L−tryptophan, tyrosine, L−fucose, L−ornithine, D−fructose, D−erythrose, and pyruvic acid are hallmark metabolites of inulin metabolized by gut microbiota. The cross-synergism network greatly indicated the microbiota ( Streptococcus , Lactobacillus , Muribaculaceae), metabolites ( l -tryptophan, pyruvic acid), and metabolism (Lysine degradation, Tryptophan metabolism , Synthesis and degradation of ketone bodies) may be the key factors involved in the mechanisms regulating the in vitro and vivo effects of inulin.
研究领域:
微生物学
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代谢组学
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营养科学
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胃肠病学
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产品关联
DOI
高影响因子
AAPS Journal
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2025
·Vol. 27
·期 1
·1-14
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IF 3.7
Wan Zhijie,Yuan Ming,Liu Ziao,Cai Yuan,He Hua,Hao Kun
The rising prevalence of metabolic-associated steatotic liver disease emphasizes the need to understand its lipid metabolism. Dapagliflozin may improve hepatic steatosis but could also increase the risk of ketoacidosis by elevating β-hydroxybutyrate (KB) levels. This study investigates dapagliflozin's effects on hepatic lipid metabolism and quantifies KB levels in vivo . Male Sprague–Dawley rats were fed either a normal diet or a high-fat diet (HFD) for 12 weeks. The HFD rats were then divided into four subgroups to receive vehicle, 0.5 mg/kg, 1 mg/kg, and 3 mg/kg of dapagliflozin for four weeks. Free fatty acids (FFA) and KB levels were monitored, while protein and gene expression were analyzed. And a dynamic model of KB was developed for humans based on preclinical data. Dapagliflozin decreased body weight and visceral fat in HFD rats, increasing KB by upregulating CPT1a, HMGCS2, and HMGCL, and downregulating ACC. These changes correlated with reduced liver/fat index, liver pathology score, and oil-red staining area. A pharmacokinetic/pharmacodynamic (PK/PD) model was created from preclinical data to quantify KB levels in rats and validated in humans. Dapagliflozin reduces hepatic steatosis by enhancing fatty acid β-oxidation and ketogenesis and inhibiting fat synthesis. A dynamic model accurately predicts ketone body levels in treated individuals. Graphical
研究领域:
药理学
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代谢性疾病
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肝病学
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营养科学
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系统生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PROTEOME RESEARCH
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2021
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IF 4.466
Peng Ma,Zijing Wang,Yisa Wang,Biyu Hou,Jialin Sun,He Tian,Bowen Li,Guanghou Shui,Xiuying Yang,Xinyu Yang,Guifen Qiang,Chong Wee Liew,Guanhua Du
Once protein synthesis is excessive or misfolded protein becomes aggregated, which eventually overwhelms the capacity of the endoplasmic reticulum (ER), a state named ER stress would be reached. ER stress could affect many tissues, especially the liver, in which nonalcoholic fatty liver disease, liver steatosis, etc. have been reported relative. However, there is still a lack of systematic insight into ER stress in the liver, which can be obtained by integrating metabolomics and transcriptomics of the tissue. Here, tunicamycin was utilized to induce ER stress in C57BL/6N mice. Microarray and untargeted metabolomics were performed to identify the genes and metabolites significantly altered in liver tissues. Surprisingly, apart from the predictable unfolded protein response, liver lipid, arginine, and proline metabolisms were affirmed to be related to ER stress. Also, the ketone body metabolism changed most prominently in response to ER stress, with few studies backing. What is more, succinate receptor 1 (Sucnr1) may be a novel marker and therapeutical target of liver ER stress. In this study, the combination of the metabolome and transcriptome provided reliable information about liver pathological processes, including key relative pathways, potential markers, and targets involved in ER stress of the liver.
研究领域:
分子生物学
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生物化学
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系统生物学
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肝病学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2024
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IF 8
Yixin Shen,Gangsheng Chen,Yi Chen,Yakun Gao,Chao Hou,Kylin Liao,Biao Ma,Hong Liu
The detection of β-hydroxybutyrate (β-HB) is crucial for the rapid diagnosis of diabetic ketoacidosis and health management. However, daily monitoring of dynamically changing β-HB concentrations is challenging and still limited to laboratory settings or painful and invasive measurements. Herein, we report a wearable electrochemical sensor integrated with iontophoresis for non-invasive monitoring of sweat β-HB. This device can actively stimulate the skin to induce sweat by iontophoresis, and the sweat is controllably collected and transported to the sensing area through the microfluidic channel. The sweat β-HB was measured through an electrochemical oxidation reaction mediated by the generated nicotinamide adenine dinucleotide, which can achieve fast response analysis of sweat β-HB within 40 seconds and a low detection limit of 10 μM. We also used this sensor to monitor the sweat β-HB levels in a volunteer experiencing the ketogenic diet and the sweat and blood β-HB levels exhibited high correlation. We believe this wearable sensor capable of on-demand and noninvasive detection of sweat β-HB shows a wide application prospect in clinical medicine and home healthcare.
研究领域:
生物医学工程
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传感器
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可穿戴技术
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糖尿病管理
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电化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）