CHINESE JOURNAL OF POLYMER SCIENCE
·
2022
·Vol. 41
·期 4
·476-482
·
IF 3.815
Zhang Jian,Zhu Jie,Hua Zan,Liu Guang-Ming
Soil environment on earth contains a variety of ions, which are expected to play a vital role in the biodegradation of plastics discarded in the environment. In this work, poly(butyleneadipate- co -terephthalate) (PBAT) is employed as a model biodegradable plastic to study the specific ion effects on the enzymatic degradation of polyester plastics. The results show that the specific ion effects on the enzymatic degradation rate of the PBAT films and on the catalytic rate constant for the enzymatic hydrolysis of the ester bonds are strongly dependent on temperature and ionic strength. Both the enzymatic degradation rate and catalytic rate constant decrease following the trends Na + > K + > Ca 2+ and Cl − > SO 4
2− > NO 3
− for cations and anions, respectively, indicating that the ion-specific enzymatic degradation of the PBAT films is closely correlated with the specific ion effects on enzymatic hydrolysis of the ester bonds. Our study shows that the specific ion effects on the enzyme activity can be understood by taking into account the ion-specific cation-anion interaction, ionic dispersion force, salting-out effect and salting-in effect. This study of specific ion effects on the enzymatic hydrolysis of the ester bonds and the resultant enzymatic degradation of the PBAT films would offer us a new clue to develop new biodegradable, environmentally friendly synthetic plastics.
研究领域:
环境科学
·
生物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF INORGANIC BIOCHEMISTRY
·
2017
·
IF 3.348
Xin Li,Zihui Liu,Ying Xu,Dongfeng Wang
A novel heterobinuclear complex [CoNa(C 15 H 10 NO 4 F) 2 (CH 3 OH)] 2 with Schiff base (C 15 H 10 NO 4 F: 2-amino-4-fluorobenzoic acid-3-methoxysalicylaldehyde) was synthesized and characterized by IR and 1 H NMR spectroscopy, elemental analysis and single crystal X-ray diffraction. X-ray crystallography reveals that the cobalt atom is six-coordinated by two nitrogen atoms from –C N–, two carboxylate oxygen atoms and two hydroxyl oxygen atoms in different ligands, while the sodium atom is seven-coordinated by two methoxy oxygen atoms, two hydroxyl oxygen atoms in different ligands, two oxygen atoms in the same carboxylate and one oxygen atom of solvent methanol. The reaction results of the complex with the p -nitrophenylphosphate (pNPP) and the adenosine monophosphate (AMP) reveal that the complex can hydrolyze phosphoester bonds. Then the DNA-hydrolysis activity is studied experimentally and theoretically, indicating that the complex can effectively hydrolyze the pBR322 supercoiled plasmid DNA . The molecular docking technology predicts the best binding site and binding affinity between the complex and DNA, and then the catalytic mechanism of hydrolysis is supposed. The study results suggest that the Schiff base metal complex, as a potent artificial enzyme , may find its applications in catalytic hydrolysis and biotechnological areas.
研究领域:
配位化学
·
生物无机化学
·
催化化学
✓ 已验证
DOI
PubMed
高影响因子
ACS Catalysis
·
2021
·
IF 13.084
Yutong Wang,Lijun Yang,Mengfan Wang,Jiaxing Zhang,Wei Qi,Rongxin Su,Zhimin He
Enzymes play vital roles in catalyzing biochemical reactions with high activity and selectivity, which is largely attributed to the delicately organized structure and groups at catalytic domains. Reconstruction of the enzymatic catalytic domains in artificial systems, to produce enzyme-like mimic, has become an attractive but challenging subject. Herein, inspired by the helical structure in the catalytic center of natural phosphatases, we created a phosphatase-like mimic through the self-assembly of de novo designed helical heptapeptides. The helical heptapeptides were elaborately decorated with well-studied catalytic groups and exhibited obvious phosphatase-like catalytic activity upon hierarchically self-assembling. In comparison with β-sheet-organized peptide assemblies, we emphasized the significance of helical structure in the hydrolysis of phosphoester bonds (over 1000 times higher in catalytic efficiency). The structure–activity relationship reveals that the phosphatase-like function attributed to the specific helical dipole moment for the binding of substrates as well as the supramolecular assembly for the formation of catalytic center. Moreover, we verified the feasibility of these helical species as a potential substitute for adenosine triphosphatase (ATPase) and alkaline phosphatase (ALPase) in some specific biological processes. This work not only presents an approach for the construction of artificial enzymes with simple peptide modules but also provides a model for primitive enzymes formed from helical short peptides that relied on self-assembly to achieve a folded structure.
研究领域:
生物化学
·
分子生物学
·
合成生物学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Luyu Shan,Jingjing Yang,Yaling Shi,Yalin Ye,Min Zhang,Ying Cui,Huijuan Yu,Yuefei Wang,Xin Chai
Various enzymes secreted by microorganisms during fermentation of Massa Medicata Fermentata (MMF) play a crucial role in conversion of the chemical substances for better clinical application. However, enzyme-driven transformation of chemical compounds during MMF fermentation has not been fully unveiled. Our findings revealed that MMF fermented with a mixture of wheat bran (WB)-flour (2:1) was observed with high enzymatic activities, alongside distinguished appearance. The activities of protease, ester bond hydrolase for cCGA and EF, and glycoside hydrolase all increased with the extended fermentation time of MMF, reaching 14.84×10 −8 ±0.3436×10 −8 , 0.05456×10 −8 ±0.0017×10 −8 , 0.5715×10 −8 ±0.1285×10 −8 , and 15.25×10 −8 ±0.01389×10 −8 Kat/g on the 8th day of fermentation. Moreover, protease has been proven to drive the conversion of WB proteins into amino acids by precolumn phenyl isothiocyanate derivatization. Besides, transformation patterns of ester bond hydrolase on substrate components were revealed, in which cryptochlorogenic acid was decomposed into caffeic acid and quinic acid, and ethyl ferulate was metabolized into ferulic acid. By glycoside hydrolase, amygdalin was transformed into prunasin and benzaldehyde. This study reveals the enzyme-mediated transformation patterns of the focused components during fermentation of MMF, laying foundation for further elucidation of its fermentation mechanism and elevation of quality control.
研究领域:
药理学
·
微生物学
·
生物化学
·
中医药
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Yan Liu,Shukun Tang,Xu Wang,Xiaoliang Wang,Xianghua Tang,Qian Wu,Zunxi Huang,Junmei Ding
The long-term and excessive use of pyrethroid pesticides poses substantial health risks and ecosystem concerns. Several bacteria and fungi have been reported that could degrade pyrethroids . The ester-bond hydrolysis using hydrolases is the initial regulatory metabolic reaction of pyrethroids. However, the thoroughly biochemical characterization of hydrolases involved in this process is limited. Here, a novel carboxylesterase, designated as EstGS1 that could hydrolyze pyrethroid pesticides was characterized. EstGS1 showed low sequence identity (<27.03%) compared to other reported pyrethroid hydrolases and belonged to the hydroxynitrile lyase family that preferred short short-chain acyl esters (C2 to C8). EstGS1 displayed the maximal activity of 213.38 U/mg at 60 °C and pH 8.5 using p NPC2 as substrate, with K m and V max were 2.21 ± 0.72 mM and 212.90 ± 41.78 µM/min, respectively. EstGS1 is a halotolerant esterase and remains stable in 5.1 M NaCl. Based on molecular docking and mutational analysis, the catalytic triad of S 74 -D 181 -H 212 and three other substrate-binding residues I 108 , S 159 , and G 75 are critical for the enzymatic activity of EstGS1. Additionally, 61 and 40 mg/L of deltamethrin and λ-cyhalothrin were hydrolyzed by 20 U of EstGS1 in 4 h. This work presents the first report on a pyrethroid pesticide hydrolase characterized from a halophilic actinobacteria.
研究领域:
环境微生物学
·
生物技术
·
酶学
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产品关联
DOI
PubMed
高影响因子
ENERGY & FUELS
·
2025
·
IF 5.3
Songqi Wang,Bairu Shi,Yang Shi,Wei-Guang Yang,Shuai Zhou,Tinglei Liu,Dongzhi Yang
Although polyacrylamide-based hydrogel exhibits excellent swelling and migration properties, it is more desirable for hydrogels with high compressive strength, flexible deformation, and rapid degradability for petroleum extraction. Herein, the core–shell structure PAM@PVA composite hydrogel achieves enhanced plugging strength through the Hofmeister effect of the PVA core and rapid degradability through the self-degrading ester bond of the flexible PAM shell, resulting in macroscopic and microscale hydrogels for oilfield fracturing and deep profile control. After the PVA network forms intramolecular hydrogen bonds by freeze–thaw and soaking in Na2SO4 brine, the PAM@PVA hydrogels reach a compressive strength of 5.62 MPa at 60% strain, which is three times that of PAM hydrogels. In the simulated fracturing plugging pressure resistance test, the macroscopic PAM@PVA hydrogels with the soft shell and hard core achieve a plugging strength of 2.48 MPa, effectively preventing migration during the plugging perforation process. At the high temperature (90 °C), the composite hydrogels completely degrade into polymer solution within 72 h due to the ester-bond hydrolysis of PAM and hydrogen-bond disruption of PVA. At the same time, the released linear polymers enhance oil recovery by 3%, resulting in up to 57% oil recovery. The high plugging strength, flexible deformation, and controllable self-degradation of PAM@PVA hydrogel offer a novel approach to improving oil recovery processes in petroleum engineering.
研究领域:
石油工程
·
高分子科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）