INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
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2017
·
IF 4.307
Hong-Yan Luo,Ma-Feng Liu,Ming-Shu Wang,Xin-Xin Zhao,Ren-Yong Jia,Shun Chen,Kun-Feng Sun,Qiao Yang,Ying Wu,Xiao-Yue Chen,Francis Biville,Yuan-Feng Zou,Bo Jing,An-Chun Cheng,De-Kang Zhu
The Gram-negative bacterium Riemerella anatipestifer CH-2 is resistant to lincosamides, having a lincomycin (LCM) minimum inhibitory concentration (MIC) of 128 µg/mL. The G148_1775 gene of R. anatipestifer CH-2, designated lnu (H), encodes a 260-amino acid protein with ≤41% identity to other reported lincosamide nucleotidylyltransferases. Escherichia coli Rosetta TM (DE3) containing the pBAD24- lnu (H) plasmid showed four- and two-fold increases in the MICs of LCM and clindamycin (CLI), respectively. A kinetic assay of the purified Lnu(H) enzyme for LCM and CLI showed that the protein could inactive lincosamides. Mass spectrometry analysis demonstrated that the Lnu(H) enzyme catalysed adenylylation of lincosamides. In addition, an lnu (H) gene deletion strain exhibited 512- and 32-fold decreases in LCM and CLI MICs, respectively. The wild-type level of lincosamide resistance could be restored by complementation with a shuttle plasmid carrying the lnu (H) gene. The transformant R. anatipestifer ATCC 11845 [ lnu (H)] acquired by natural transformation also exhibited high-level lincosamide resistance. Moreover, among 175 R. anatipestifer field isolates, 56 (32.0%) were positive for the lnu (H) gene by PCR. In conclusion, Lnu(H) is a novel lincosamide nucleotidylyltransferase that inactivates LCM and CLI by nucleotidylylation, thus conferring high-level lincosamide resistance to R. anatipestifer CH-2.
研究领域:
微生物学
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分子生物学
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抗生素抗性研究
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Dezhi Shi,Jing Ji,Yue Wang,Lirong Lu,Shaohui Zhong,Zhifeng Tang,Yunyu Zhang,Wufeng Gao,Meng Cao,Yang Hu,Yunfeng Zheng,Shiwen Huang,Jianming Cheng
The industrial enrichment of fat-soluble compounds is a common challenge, which is also present in Piper wallichii (Miq.) Hand.-Mazz. (PWH). The stem of PWH is rich in amide alkaloid substances, although enrichment methods have been available for total amide alkaloids, their water-insoluble character has not yet been addressed by a green and efficient method. We have elaborated the adsorption characteristics of pellitorine and futoamide on macroporous resins. Synchronization of concentration and adsorption was used to solve the critical problem of enrichment on macroporous resins with lipid-soluble compounds that tend to clog the resin columns during the enrichment process. Fifteen batches of PSA (amide alkaloids of PWH) were prepared from PWH collected from multiple origins, and multidimensional fingerprints were established in combination with multivariate statistical analysis to evaluate the consistency and variability of the compounds in multiple batches. Then, the fingerprints were correlated with their corresponding antioxidant activities to identify the pharmacodynamic compounds, thus clarifying the key compounds. Finally, compound separation was performed to verify the accuracy of the antioxidant activity of the key compounds and fingerprint-efficacy correlation model. HP-20 macroporous adsorbent resin has optimal adsorption and desorption which was selected for the enrichment of PSA. The adsorption of PSA, including futoamide and pellitorine, on the HP-20 resin was consistent with the Pseudo-second order kinetic model and Langmuir isotherm model, and this adsorption was a spontaneous exothermic entropy increase mechanism. The on-column adsorption process was optimized to solve the phenomenon that the precipitation of fat-soluble amides were easy to block the glass column during the enrichment process of the macroporous adsorption resin, and the effective enrichment of PSA was achieved. The content of pellitorine and futoamide was 13.95 g/100g and 3.11 g/100g in prepared samples respectively. The percentage of PSA was more than 50 g/100g. Seventeen differentiated compounds were screened by multivariate statistical analysis, antioxidant activity of 4 individual compounds isolated by fingerprint-efficacy correlation such as pellitorine were validated. The present study provided a more effective method for the enrichment of PSA, and initially elucidated the active compounds of the antioxidant activity of PWH, which will establish the basis for the industrialization of PSA in the future.
研究领域:
植物化学
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药理学
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分析化学
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DOI
高影响因子
International Journal of Environmental Research and Public Health
·
2022
·Vol. 19
·期 13
·7985
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IF 4.614
Xiaoning Wang,Yangyang Sun,Qian Wang,Fengying Liu,Weijie Yang,Xin Sui,Jun Yang,Minmin Zhang,Shuai Wang,Zhenyu Xiao,Yuan Luo,Yongan Wang,Tong Zhu
The “Multi-Threat Medical Countermeasure (MTMC)” strategy was proposed to develop a single drug with therapeutic efficacy against multiple pathologies or broad-spectrum protection against various toxins with common biochemical signals, molecular mediators, or cellular processes. This study demonstrated that cytotoxicity, expression of transient receptor potential cation channel subfamily A member 1 (TRPA1) mRNA, and intracellular calcium influx were increased in A549 cells exposed to amide herbicides (AHs), in which the order of cytotoxicity was metolachlor > acetochlor > propisochlor > alachlor > butachlor > propanil > pretilachlor, based on IC50values of 430, 524, 564, 565, 619, 831, and 2333 μM, respectively. Inhibition/knockout of TRPA1 efficiently protected against cytotoxicity, decreased TRPA1 mRNA expression, and reduced calcium influx. The results suggested that the TRPA1 channel could be a key common target for AHs poisoning. The order of TRPA1 affinity for AHs was propanil > pretilachlor > metolachlor > (propiso/ala/aceto/butachlor), based on KDvalues of 16.2, 309, and 364 μM, respectively. The common molecular mechanisms of TRPA1-AHs interactions were clarified, including toxicity-effector groups (benzene ring, nitrogen/oxygen-containing functional groups, halogen) and residues involved in interactions (Lys787, Leu982). This work provides valuable information for the development of TRPA1 as a promising therapeutic target for broad-spectrum antitoxins.Keywords:amide herbicides;TRPA1 channel;cytotoxicity;computational simulation;common mechanisms
研究领域:
毒理学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
Foods
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2023
·Vol. 12
·期 24
·4392
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IF 5.2
Rui Wang,Chaolong Rao,Qiuyan Liu,Xiong Liu
Zanthoxylumoleoresin, a concentrated extract derived fromZanthoxylum bungeanum, is rich in non-volatile, intensely flavorful substances and amide compounds, such as hydroxy-α-sanshool, hydroxy-β-sanshool, and hydroxy-ε-sanshool. The production process ofZanthoxylumoleoresin remains unstandardized, and there is still a lack of research on the precise classification and quantification of its key chemical constituents, as well as the stability of these compounds when produced using different extraction methods. This study utilized preparative liquid chromatography to extract and purify amide compounds fromZanthoxylumoleoresin, successfully isolating three sanshools: hydroxy-α-sanshool, hydroxy-β-sanshool, and hydroxy-ε-sanshool. The stability of three these sanshools under UVB irradiation in different solvents was explored in UVB-simulated sunlight conditions to investigate the degradation or transformation mechanism ofZanthoxylumalkylamides under UVB irradiation. The findings indicate a rapid decrease in the hydroxy-α-sanshool content under UVB ultraviolet light, aligning with the second-order kinetics. This study revealed alterations in the contents of hydroxy-α-sanshool, hydroxy-β-sanshool, and hydroxy-ε-sanshool and the formation of a new compound following exposure to UVB light. This new compound, along with the three sanshools, possesses a uniformm/z264 and shares similar chemical structures. Further analysis also uncovered that these compounds are capable of undergoing isomerization reactions under UVB irradiation. This demonstrates that UVB irradiation of certain intensities can modify the concentrations and chemical structures of theseZanthoxylumalkylamides. These insights offer crucial guidance for future studies on the processing and preservation ofZanthoxylumalkylamides and their derivatives.Keywords:Zanthoxylumalkylamides;UVB light;degradation;transformation;mechanism
研究领域:
食品科学
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植物化学
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PubMed
高影响因子
FOOD CHEMISTRY
·
2020
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IF 6.306
Hengye Chen,Ou Hu,Yao Fan,Lu Xu,Lei Zhang,Wei Lan,Ying Hu,Xingrui Xie,Lixia Ma,Yuanbin She,Haiyan Fu
The needing of rapid and sensitive detection method for pesticides is increasing, to facilitate its detection without complicated instruments. Herein, a novel paper-based senor was developed for the visual detection of three carbamate pesticides (metolcarb, carbofuran, and carbaryl) based on CdTe quantum dots (QDs) and nano zinc 5, 10, 15, 20-tetra(4-pyridyl)-21 H -23 H -porphine (nano ZnTPyP) with a “turn-off-on” mode. This fluorescence sensing model could be applied in the highly selective and sensitive detection of carbamate pesticides both by fluorescence spectrometry or paper-based sensors. Based on the extracted RGB color values of paper, the partial least squares regression (PLSR) was used to accurately quantify the concentrations of carbamate pesticides in different food matrices (apple, cabbage and tea water). This method featured in high speed, low price and high accuracy, and provided a new strategy for the detection of food safety.
研究领域:
食品安全检测
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分析化学
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环境科学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
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2024
·Vol. 192
·期 1
·1-12
·
IF 5.3
Su Lin,Wu Zhuqiang,Ruan Guihua,Huang Yipeng
Polymeric high internal phase emulsions decorated with covalent organic frameworks (polyHIPEs-COFs) were synthesized and used as the sorbent for cyantraniliprole and chlorantraniliprole. Pickering high internal phase emulsions stabilized by covalent organic frameworks solid particles and liquid surfactants (Span80 and polyvinylpyrrolidone) endow the composites with open-cell structures and superwettability. The amphiphilicity and open-cell structures enable rapid adsorption and desorption for cyantraniliprole and chlorantraniliprole, and the solid-phase extraction process can be completed in 5 min. The adsorption efficiencies of polyHIPEs-COFs for cyantraniliprole and chlorantraniliprole are above 85.19%, but lower than 10% for fenvalerate, anti-aphid, and chlorpyrifos, demonstrating the good adsorption selectivity for cyantraniliprole and chlorantraniliprole. The adsorption efficiencies of cyantraniliprole and chlorantraniliprole using a same polyHIPEs-COFs and five different batches of polyHIPEs-COFs range from 94.25 to 100.00%, revealing the good reproducibility of the sorbent. In addition, the polyHIPEs-COF-based solid-phase extraction combined with high-performance liquid chromatography-ultraviolet detector (HPLC–UV) was developed for determination of bisamide insecticides in vegetable (eggplants, tomatoes, and peppers) samples. Results showed that the method was feasible to determine the cyantraniliprole and chlorantraniliprole in real vegetable samples with a linear range of 0.012–1.2 μg/kg and limits of detection of 0.0075–0.0090 μg/kg. The recoveries of cyantraniliprole and chlorantraniliprole spiked in vegetable samples ranged from 85.00 to 100.00% with relative standard deviations less than 3.52%. The study indicates the feasibility of amphiphilic polyHIPEs-COFs in extraction and enrichment of bisamide insecticides from vegetable samples for HPLC–UV analysis. Graphical
研究领域:
分析化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
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2024
·
IF 3.4
Rilong Liu,Hangzhen Lan,Song Yan,Lu Huang,Daodong Pan,Yichun Wu
An immunoassay method based on penicillin-binding protein (PBP) was developed for the quantitative determination of 10 kinds of beta-lactam antibiotics (BLAs). First, two kinds of PBPs, which are named PBP1a and PBP2x, were expressed and purified, and they were characterized by SDS-PAGE and western blotting analysis. Then, the binding activity of PBP1a and PBP2x to template BLAs, cefquinome (CEFQ) and ampicillin (AMP), was determined. The effect of the buffer solution system, e.g., pH, ion concentration, and organic solvent, on the immune interaction efficiency between PBPs and BLAs was also evaluated. In the end, the PBP-based immunoassay method was developed and validated for the detection of 10 kinds of BLAs. Under optimal conditions, PBPs exhibited high binding affinity to BLAs. In addition, this method showed a high sensitivity for the detection of 10 kinds of BLAs with the limits of detection from 0.21 to 9.12 ng/mL, which are much lower than their corresponding maximum residual limit of European Union (4–100 ng/mL). Moreover, the developed PBP-immunoassay was employed for BLA detection from milk samples, and satisfactory recoveries (68.9–101.3 %) were obtained.
研究领域:
生物化学
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药物分析
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食品科学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Zhishuang Yang,Tianjing Lan,Hongyan Luo,Pei Li,Mingshu Wang,Renyong Jia,Shun Chen,Mafeng Liu,Xinxin Zhao,Qiao Yang,Ying Wu,Shaqiu Zhang,Juan Huang,Xumin Ou,Sai Mao,Qun Gao,Di Sun,Bin Tian,Anchun Cheng,Dekang Zhu
Antimicrobial resistance remains an utmost concern in human and veterinary medicine, impacting humans, animals, and the environment while significantly influencing the principles of One Health. While Riemerella anatipestifer ( R. anatipestifer ) is recognized as a waterfowl pathogen with multidrug-resistant properties, the specifics of its lincosamide resistance mechanism are inadequately understood. In this study, we identified a novel lincosamide resistance gene, lnu (I), in R. anatipestifer RCAD0121, and investigated its potential origin, transfer mechanisms, and dissemination status through genomic epidemiology. This exhibited 74.80 % amino acid identity with a previously reported gene, lnu (H). PCR analysis revealed lnu (I) prevalence in at least 44 R. anatipestifer isolates collected from multiple provinces in China. Furthermore, genomic mining unveiled 56 lnu (I) sequences within publicly available databases, primarily originating from environmental sources. In addition, members of the family Flavobacteriaceae were the dominant (16/56, 28.57 %) bacteria carrying the lnu (I) gene, with Flavobacterium exhibiting a similar GC content as lnu (I). Notably, specific instances of the lnu (I) gene were linked to mobile genetic elements within human and animal pathogenic bacteria. These findings suggest that Flavobacterium species within the environment could serve as potential ancestral sources of the novel lnu (I) gene, which has undergone mobilization events toward pathogenic bacteria.
研究领域:
微生物学
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分子生物学
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医学
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基因组学
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DOI
PubMed
高影响因子
TALANTA
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2017
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IF 4.162
Wenhua Ji,Rihan Sun,Wenjuan Duan,Xiao Wang,Tao Wang,Yan Mu,Lanping Guo
In this study, a novel amphiphilic magnetic molecularly imprinted polymers (MMIPs) have been prepared by using Fe 3 O 4 microspheres as the magnetic core, 4-vinyl pyridine (4-VP) and alkenyl glycosides glucose (AGG) as functional co-monomers. Fe 3 O 4 microspheres were directly encapsulated by the polymer without any surface modification in the distillation-precipitation polymerization. The morphology and composition of MMIPs were characterized by X-ray diffraction (XRD), vibrating sample magnetometry (VSM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Binding property and magnetic separation ability were systematically investigated through the equilibrium binding experiments. The feasibility of magnetic molecular imprinted solid phase extraction (MMISPE) was investigated for the selective enrichment of chloroacetamide herbicides from environmental water samples. The developed MMISPE-HPLC method exhibited good linearity (0.1–200 μg L −1 ), low limit of detection (0.03–0.06 μg L −1 ), and good precision (RSD<7%) under the optimized conditions. The introduced MMISPE-HPLC method was successfully used to analyze chloroacetamide herbicides in environmental water samples. Spiked chloroacetamide herbicides recoveries in three water samples ranged from 82.1% to 102.9%. These results indicated that amphiphilic MMIPs were the promising sorbents for the selective enrichment of chloroacetamide herbicides at trace levels from real environmental water samples.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
Nanoscale
·
2015
·Vol. 7
·期 39
·16381-16388
·
IF 7.394
Yanni Chen,Yongwei Wang,Liqiang Liu,Xiaoling Wu,Liguang Xu,Hua Kuang,Aike Li,Chuanlai Xu
A novel gold immunochromatographic assay (GICA) based on anti-β-lactam receptors was innovatively developed that successfully allowed rapid and simultaneous detection of fifteen β-lactams in milk samples in 5–10 minutes. By replacing the antibodies used in traditional GICA with anti-β-lactam receptors, the difficulty in producing broad specific antibodies against β-lactams was overcome. Conjugates of ampicillin with BSA and goat anti-mouse immunoglobulin (IgG) were immobilized onto the test and control lines on the nitrocellulose membrane, respectively. Since goat anti-mouse IgG does not combine with receptors, negative serum from mice labelled with gold nanoparticles (GNP) was mixed with GNP-labelled receptors. Results were obtained within 20 min using a paper-based sensor. The utility of the assay was confirmed by the analysis of milk samples. The limits of detection (LOD) for amoxicillin, ampicillin, penicillin G, penicillin V, cloxacillin, dicloxacillin, nafcillin, oxacillin, cefaclor, ceftezole, cefotaxime, ceftiofur, cefoperazone, cefathiamidine, and cefepime were 0.25, 0.5, 0.5, 0.5, 1, 5, 5, 10, 25, 10, 100, 10, 5, 5, and 2 ng mL−1, respectively, which satisfies the maximum residue limits (MRL) set by the European Union (EU). In conclusion, our newly developed GICA-based anti-β-lactam receptor assay provides a rapid and effective method for one-site detection of multiple β-lactams in milk samples.
研究领域:
分析化学
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食品安全
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 15
·6411-6418
·
IF 1.821
Yan Wang,Xiaoyang Jin,Dan Zhao,Xiaohong Guo,Ranhong Li
A molecularly imprinted solid phase extraction (MISPE) coupled with gas chromatography (GC) for the simultaneous separation and determination of four chloroacetamide herbicides, alachlor, acetochlor, pretilachlor and metolachlor, in soil was developed. Molecularly imprinted polymers (MIPs) with butachlor as the dummy template were synthesized via precipitation polymerization, where methacrylic acid (MAA) was used as the functional monomer, ethylene glycol dimethacrylate (EDMA) as the crosslinker and acetonitrile as the porogen. Fourier-transform infrared spectrometry (FTIR) and scanning electron microscopy (SEM) were used to characterize the polymers. Under the optimum SPE conditions, the polymer sorbents can selectively extract and enrich alachlor, acetochlor, pretilachlor and metolachlor. A comparison experiment also showed that the MISPE cartridges were better than the nonimprinted and C18 cartridges in terms of recovery. The mean recoveries of the four chloroacetamide herbicides from blank soil spiked at 0.1, 0.5, and 1 μg mL−1 ranged between 80.6 and 90.2% with relative standard deviations (RSD) of less than 8% (n = 5). The limit of detection (LOD) and the limit of quantification (LOQ) of the four analytes were in the range of 1.0 × 10−12 to 5 × 10−11 g and 0.0005–0.025 mg kg−1, respectively. The presented MISPE-GC method exhibited highly selective separation and enrichment of trace chloroacetamide herbicides and could be potentially applied to the determination of chloroacetamide herbicides in soil.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
ACS Applied Energy Materials
·
2021
·
IF 6.024
Gaopan Liu,Tianpeng Jiao,Yong Cheng,Ke Zhou,Yue Zou,Mingsheng Wang,Yong Yang,Jianming Zheng
A silicon-based anode is being developed for industrial application in lithium-ion batteries (LIBs) because of its extremely high theoretical specific capacity. However, the large volume variation during de/lithiation and poor interfacial stability issues cause rapid capacity decay, hindering its practical application. Herein, from the interfacial stabilization point of view, a lactam-based electrolyte additive, namely, N-acetylcaprolactam (donated as NACA), is explored to construct a flexible, robust, and protecting solid electrode interphase (SEI) on the SiOx/C electrode surface. On one hand, due to a stronger complexation with PF5/HF/H2O, NACA functions well in stabilizing the LiPF6 salt, thereby mitigating the corrosion of HF toward the SiOx/C electrode. On the other hand, the NACA-derived interphase could be well adapted to the huge volume change of the silicon electrode. As a consequence, the Li||SiOx/C battery with 2% NACA shows an increase in capacity retention by 15.6% at 30 °C and by 20.8% at 45 °C at 0.5C compared to the baseline. Meanwhile, the cycling performance of the NCM90 cathode is also improved with the NACA additive. Our work provides valuable understanding for seeking more effective amide-based additives for a silicon-based electrode and for the development of functional electrolytes for high-energy-density LIBs.
研究领域:
材料科学
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电化学
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能源存储
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DOI
高影响因子
危险品化学品经营许可证（带存储）