VETERINARY MICROBIOLOGY
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2022
·
IF 3.246
Hongyan Luo,Mengru Li,Zhishuang Yang,Dekang Zhu,Pei Li
A novel chromosomally-located β-lactamase gene, bla RASA-1 , was identified in Riemerella anatipestifer RA-CH-1. The RASA-1, encoded by bla RASA-1 , was a class A extended-spectrum β-lactamase (ESBL), which shared 42.7% and 40.5% identities with the RAA-1 and CGA-1 β-lactamase, respectively. Overexpression of RASA-1 in Escherichia coli confers broad resistance to β-lactams and the purified native RASA-1 revealed ESBL-like hydrolysis activity. Blasting in GenBank showed that bla RASA-1 was exclusively detected in Riemerella anatipestifer . Moreover, sequence analysis revealed that this gene was located within the multi-resistance region of Riemerella anatipestifer genome.
研究领域:
微生物学
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抗药性研究
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Huibing Chi,Bingjie Xia,Juan Shen,Xiaoyu Zhu,Zhaoxin Lu,Fengxia Lu,Ping Zhu
Type II L-asparaginase as a pivotal enzyme agent has been applied to treating for acute lymphoblastic leukemia (ALL) and efficient mitigation of acrylamide formed in fried and baked foods. However, low activity, narrow range of pH stability, as well as undesirable glutaminase activity hinder the applications of this enzyme. In our work, A novel type II L-asparaginase (CgASNase) from Corynebacterium glutamicum with molecular mass of about 35 kDa was chosen to express in E. coli . CgASNase shared only 27 % structural identity with the reported L-asparaginase from Helicobacter pylori . The purified CgASNase showed the highest specific activity of 1979.08 IU mg −1 to L-asparagine, compared with reported type II ASNases in the literature. CgASNase displayed superior stability at a wide pH range from 5.0 to 11.0, and retained about 76 % of its activity at 30 °C for 30 min. The kinetic parameters K m (Michaelis constant), k cat (turnover number), and k cat / K m (catalytic efficiency) values of 4.66 mM, 79,697.40 min −1 , and 17,102.45 mM −1 min −1 , respectively. More importantly, CgASNase exhibited strict substrate specificity towards L-asparagine, no detectable activity to l -glutamine. To explore its ability to catalyze L-asparagine, CgASNase was supplied in frying potato chips, which produced the fries with 84 % less acrylamide content compared with no supply. These findings suggest that CgASNase presents excellent properties for chemotherapy against diseases and great potential in the food processing industry.
研究领域:
生物化学
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酶学
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食品科学
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
Antibiotics-Basel
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2025
·Vol. 14
·期 9
·915
·
IF 4.6
Ru Wang,Kun Mi,Aihua Lu,Chengyang Zhang,Lei Sun,Yuxiang Chen,Yuanhu Pan,Yanfei Tao,Lingli Huang
Background/Objectives: Extended-spectrum β-lactamase (ESBL)-producingEscherichia coliposes a significant global health challenge, as it leads to antimicrobial treatment failure and is associated with elevated mortality rates. The use of β-lactam/β-lactamase inhibitor combinations offers an alternative approach for combating ESBL-producing bacteria. Ceftriaxone (CRO) and sulbactam have been coadministered in the clinical settings; however, discrepancies in their pharmacokinetics raise concerns regarding the rationality of this combination.Methods: This study was designed to investigate the postβ-lactamase inhibitor effect (PLIE) under both static and dynamic conditions, with the aim of supporting the clinical application of this combination.Results: The minimum inhibitory concentration (MIC) of CRO/SBT (2:1 ratio) againstE. coliNCTC 13353 was determined to be 32/16 μg/mL. The PLIEs were determined to be −1.26, −0.57, and 0.37 h at CRO/SBT concentrations ranging from 1/2 MIC to 2 MIC, respectively. The results of CRO concentration, β-lactamase activity,blaCTX-M-15expression, and cell morphology collectively support that SBT exerts PLIEs and protects against the antibacterial activity of CRO. In the dynamic hollow-fiber infection model, CRO monotherapy showed no inhibitory effect onE. coli, whereas CRO/SBT combination therapy rapidly eliminated SBT, achieved comparable bactericidal effects, prolonged CRO exposure, and maintained low β-lactamase activity levels.Conclusions: In conclusion, CRO/SBT exerts an inhibitory effect on enzyme-producing strains by being able to produce PLIE to maintain the inhibition of β-lactamase.
研究领域:
微生物学
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感染病学
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药理学
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临床药学
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抗菌药物治疗
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分子生物学
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转化医学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Optical Materials
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2023
·
IF 0
Jie Zhu,Yang Sun,Meng-Xin Duan,Zheng-Wu Wang,Xin Tan,Zhen Yu,He-Fang Wang
The fast and accurate detection of β-lactamase is a prerequisite of customized treatments and food safety. We present a pretreatment-free dual-excitation three-mode detection of β-lactamase by using ZnAl2O4:Cr3+ etched by ethylenediaminetetraacetic acid disodium (ZAO-EDTA) as a phosphorescent probe and nitrocefin as the signal mediator. The changes in the wavelength overlaps of UV–vis absorbance of nitrocefin upon interactions with β-lactamase and the dual excitation of ZAO-EDTA enable the design of three detection modes: i.e., the enhanced emission when excited at 393 nm, the quenched emission when excited at 533 nm, and the ratio of enhanced excitation of 393 nm and decreased excitation of 533 nm to determine β-lactamase. All three modes have a wide linear range, high sensitivity and selectivity, and fine anti-interference capability. The lowest limit of detection is observed in the ratiometric excitation mode (0.2 U·L–1), and the proposed method can directly detect β-lactamase in bacteria and milk samples without any pretreatments, with the spiked recoveries of 95.3–113% and relative standard deviation < 3.1%. Therefore, the proposed method would be attractive for the detection of β-lactamase in a complex sample, and the ZAO-EDTA probe can be widely used to establish other excitation ratiometric optosensings by changing other signal mediators.
研究领域:
分析化学
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生物传感
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食品安全
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微生物学
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DOI
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Yawen Zhou,Juan Shen,Huibing Chi,Xiaoyu Zhu,Zhaoxin Lu,Fengxia Lu,Ping Zhu
Type II L-asparaginase (ASNase) has been approved by the FDA for treating acute lymphoid leukemia (ALL), but its therapeutic effect is limited by low catalytic efficiency and L-glutaminase (L-Gln) activity. This study utilized free energy based molecular dynamics calculations to identify residues associated with substrate binding in Bacillus licheniformis L-asparaginase II (BLASNase) with high catalytical activity. After saturation and combination mutagenesis , the mutant LGT (74 L/75G/111 T) with intensively reduced l -glutamine catalytic activity was generated. The l -glutamine/L-asparagine activity (L-Gln/L-Asn) of LGT was only 6.6 % of parent BLASNase, whereas the L-asparagine (L-Asn) activity was preserved >90 %. Furthermore, structural comparison and molecular dynamics calculations indicated that the mutant LGT had reduced binding ability and affinity towards l -glutamine. To evaluate its effect on acute leukemic cells, LGT was supplied in treating MOLT-4 cells. The experimental results demonstrated that LGT was more cytotoxic and promoted apoptosis compared with commercial Escherichia coli ASNase. Overall, our findings firstly provide insights into reducing l -glutamine activity without impacting L-asparagine activity for BLASNase to possess remarkable potential for anti-leukemia therapy.
研究领域:
生物化学
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药理学
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分子生物学
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癌症研究
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产品关联
DOI
PubMed
高影响因子
International Journal of Environmental Research and Public Health
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2013
·Vol. 10
·期 7
·2688-2698
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IF 1.998
Wenbin Wang,Liqiang Liu,Liguang Xu,Wei Ma,Hua Kuang,Chuanlai Xu
β-Lactamase residues in milk represent a public health risk. The cylinder plate detection method, which is based on bacterial growth, is laborious and time consuming. In this study, 15 monoclonal antibodies (mAbs) were selected against Temoneira (TEM) 1 β-lactamase. A sandwich enzyme-linked immunosorbent assay (ELISA) based on an optimum mAb pair was developed and validated for the detection of β-lactamase. The limit of detection and linear dynamic range of the method were 4.17 ng/mL and 5.5–100 ng/mL, respectively. β-Lactamase recovery in pure milk was 96.82–103.13%. The intra- and inter-assay coefficients of variation were 6.21–7.38% and 12.96–13.74%, respectively. Our developed sandwich ELISA can be used as a rapid detection method of β-lactamase in milk.
研究领域:
食品科学
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免疫学
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公共卫生
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2020
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IF 3.232
Pan Yu,Jia-Ning Yang,Jin-Wu Yan,Zhi-Zhong Meng,W. David Hong,Adam P. Roberts,Stephen A. Ward,Lei Zhang,Shan Li
The rapid detection of β-lactamases (Blas) and effective screening of Bla inhibitors are critically important and urgent for solving antibiotic resistance and improving precision medicine. Here a novel fluorescent probe CDC-559 was designed and synthesized, which can be used for the selective and direct detection of AmpC Blas. More importantly, it can realize screening the Bla inhibitors with sulbactam sodium and tazobactam as model compounds, and the half-maximal inhibitory concentration are 0.279 μM and 0.053 μM, respectively. CDC-559 can be applied not only to examine the resistance of bacterial strains, but also to categorize its mode of action specifically, which is consistent with the essential result of the Blas. The research suggests that CDC-559 probe has tremendous potential in the rapid detection of AmpC Blas as well as the strains with AmpC-encoded gene, which is instructive in promoting better antibiotic stewardship practices and developments.
研究领域:
化学
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药理学
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微生物学
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生物医学工程
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2025
·
IF 5.2
Wentao Zhou,Caini Yu,Tingting Meng,Qi Jiang,Fangying Yu,Hong Yuan
Cellular senescence disrupts tissue homeostasis and diminishes physiological integrity, leading to the accumulation of senescent cells (SCs) in multiple senescence‐associated diseases such as chronic kidney disease (CKD). Treatment of SCs has been approved to be a feasible approach to these diseases. However, curing SCs in different cell types remains challenging. In this study, we leveraged the high expression of glutaminase (GLS) in SCs to develop a drug delivery system utilizing γ-poly glutamic acid (γ-PGA) conjugated with octadecylamine (ODA) to encapsulate rapamycin (RP), resulting in a GLS-responsive drug delivery system, designated as RPPO. In a model of drug induced senescence, the γ-PGA component of RPPO was degraded by cellular GLS, facilitating the release of encapsulated RP and rejuvenating SCs by restoring the autophagic capacity. Additionally, in a model of CKD in mice, RPPO enhanced recovery by rejuvenating SCs, reducing fibrosis, and alleviating inflammation. Thus, this senescent cell-responsive drug delivery system presents a novel approach for the treatment of CKD.
研究领域:
医学
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药学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Foods
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2020
·Vol. 9
·期 4
·503
·
IF 4.092
Yanli Ma,Ruijin Yang,Wei Zhao
Gelatin is a promising candidate for making bioplastic film; however, the water soluble property has limited its applications. Here, we have successfully fabricated a water-insoluble gelatin film with the assistance of biocatalysis. This innovative gelatin film could retain its original shape at ambient temperature (30 °C) or even in boiling water. Type E gelatin could form more covalent crosslinks when compared to that of conventional ones with the same amount of microbial transglutaminase (MTGase), and it exhibits obvious changes in terms of molecular weight, network structure, and mechanical strength. This work could provide a strategy for fabricating water-insoluble gelatin film and open routes for the development of bioplastic film using gelatin.Keywords:gelatin;glutamine;microbial transglutaminase;crosslink;water-insoluble
研究领域:
材料科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2013
·Vol. 18
·期 11
·13910-13919
·
IF 2.428
Wen-Jian Xia,Zong-Bo Xie,Guo-Fang Jiang,Zhang-Gao Le
An environmentally-friendly, enzyme-promoted procedure for the Henry reaction was first studied using water-in-[Bmim][PF6] microemulsions as reaction medium. The Amano acylase fromAspergillus oryzaeshowed better catalytic activity for the addition reactions of nitromethane with a series of aromatic aldehydes, and a highest yield of 90% was obtained.Keywords:Henry reaction;acylase;water-in-[Bmim][PF6] microemulsion;enzyme promiscuity
研究领域:
生物化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2023
·
IF 8.3
Baihui Chi,Fei Li,Xukang Wang,Haixia Pan,Xianliang Yi,Yang Liu,Jingjing Zhan,Xuwang Zhang,Hao Zhou,Wenyuan Wang
N,N-dimethylformamide (DMF) is widely used in various industries, but its direct release into water poses high risks to human beings. Although a lot of DMF-degrading bacteria has been isolated, limited studies focus on the degradation preference among DMF and its analogues. In this study, an efficient DMF mineralization bacterium designated Aminobacter ciceronei DMFA1 was isolated from marine sediment . When exposed to a 0.2% DMF (∼1900 mg/L), strain DMFA1 exhibited a degradation efficiency of 100% within 4 days. The observed growth using formamide as the sole carbon source implied the possible DMF degradation pathway of strain DMFA1. Meanwhile,the strain DMFA1 possesses a broad-spectrum substrate degradation, which could effectively degraded 0.2% N,N-dimethylacetamide (DMAC) and N-methylformamide (NMF). Genomic analysis further confirmed the supposed pathway through annotating the genes encoding N, N-dimethylformamidase (DMFase), formamidase, and formate dehydrogenase . The existence of sole DMFase indicating its substrate specificity controlled the preference of DMAc of strain DMFA1. By integrating multiple sequence alignment, homology modeling and molecular docking, the preference of the DMFase in strain DMFA1 towards DMAc are related to: 1) Mutations in key active site residues; 2) the absence of small subunit; and 3) no energy barrier for substrates entering the active site.
研究领域:
环境微生物学
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生物降解
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生物化学
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2016
·
IF 3.388
Jing Qin,Xiping Cui,Panpan Wu,Zhengyun Jiang,Yingshan Chen,Ruili Yang,Qingqing Hu,Yuanming Sun,Suqing Zhao
Fluorescent sensor assay (FSA) was developed by using a fluorescein-labeled aptamer assembled onto Graphene oxide (GO) in order to determine β-lactamase in milk. Under optimal conditions, FSA indicated a detection range from 1 to 46 U/mL with a limit of detection (LOD) of 0.5 U/mL ( R 2 = 0.999, n = 3). In addition, commercial milk samples tainted with β-lactamase were detected by the established FSA with a recovery rate between 96.04 and 119.67%. Additionally, the reliability and sensitivity of FSA was validated by enzyme-linked immunoassay (ELISA) with a high correlation of 0.993. Thus, these data, combined with the ease and speed of the assay, suggest that the developed FSA may represent a promising method for monitoring β-lactamase contamination in milk.
研究领域:
生物化学
·
食品科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）