Biomass Conversion and Biorefinery
·
2022
·Vol. 13
·期 5
·4343-4351
·
IF 4.05
Liu Wei,Na Haining,Hu Changhui,Zhou Fang,Zhang Zhenyu,Guo Zhuo,Li Mian,Zhu Jin
With the aim to exhibit the advantages of catalytic accelerator in enhancing the selective hydrolysis of cellulose to sugar, cellulose is hydrolyzed in low acid aqueous system with catalytic accelerator. And then sugar products are extracted and fermented by yeast. By the assistance of catalytic accelerator, cellulose conversion rate and total reducing sugar yield are 95.7% and 90.9%. And the glucose yield is as high as 73.6%. The sugar products with high glucose content can be easily extracted from cellulose hydrolysate. Low toxicity of the sugar products to yeast is found during yeast fermentation. The glucose in cellulose hydrolysate can be fully consumed after 12 h. A highly actual ethanol yield is exhibited, which is equal to the actual yield level of standard glucose solution. The important role of catalytic accelerator in aqueous hydrolytic system to produce fermentable sugar is clearly reflected. Furthermore, a simple but effective way is established to efficient utilization of cellulose as an applicable carbon source for the conversion of more value-added chemicals and materials.
研究领域:
生物化学
·
可再生资源利用
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Caiyun Chu,Dayu Zhou,Yuwei Men,Jialei Wang,Shiyuan Zhang,Hongliang Fan,Ying He,Xiaohui Zhao,He Liu
This study explored how yeast fermentation can reduce purine content in soy whey and regulate associated metabolic pathways. By combining both targeted and untargeted metabolomics, we examined changes in purines, amino acids, and organic acids during fermentation using different yeast strains. Among them, one strain significantly reduced the total purine content in soy whey by over 35 %. We observed that fermentation influenced the balance of key nutrients and bioactive compounds, including sugars and organic acids. Further analysis revealed that the yeast modified central metabolic pathways, such as purine metabolism, amino acid metabolism, and energy production. These findings provide new insights into the mechanisms of purine degradation during fermentation and suggest a promising strategy for developing low-purine functional foods using soy whey as a nutritional substrate.
研究领域:
食品科学
·
微生物学
·
生物化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2025
·
IF 3.3
Yu Xiaohong,Zhang Ya,Wang Xianhao,Luo Yutong,Shao Shuai,Qiu Zhongyang
Organic acid pretreatment is one of the most promising methods to pretreat lignocellulose biomass due to its mild condition and weak corrosion to reactor. In this study, five different organic acids were used for wheat bran pretreatment, and the enzymatic hydrolysis yields of pretreated feedstocks were evaluated. Among these organic acids, oxalic acid pretreatment demonstrated the highest glucose yield of 87.93%. Subsequently, the optimization of oxalic acid pretreatment parameters was conducted including temperature, time, solid–liquid ratio, and acid usage. A high glucose yield of 96.92% was obtained at the optimal conditions: 0.8% of oxalic acid usage, 1:10 of solid–liquid ratio, and 130 ℃ for 15 min. FTIR, XRD and SEM revealed the mechanism of improved hydrolysis efficiency after oxalic acid pretreatment. Finally, the pretreated wheat bran was used for separate hydrolysis and ethanol fermentation (SHF) at 20% (w/w) solids loading. After 36 h, 23.87 g/L ethanol was produced by Saccharomyces cerevisiae DQ1 with all glucose consumed, with the yield of 0.12 g/g dry virgin wheat bran. This study provided a new insight into wheat bran pretreatment for biofuel production by a mild oxalic acid pretreatment method.
研究领域:
生物技术
·
可再生能源
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Saudi Chemical Society
·
2024
·
IF 5.8
Liping Zhao,Ye Zhao,Lihe Zhao,Gang Liu
A nitrogen-phosphorus dual-doped auricularia auricula carbon (NP-AC) matrix was prepared through hydrothermal treatment using natural auricularia auricula as raw material and medium solution. Yeast was introduced and cultured by the auricularia auricula liquid medium. The NP-AC/S composite was prepared through further freeze-drying, carbonization, activation and compounding with sulfur procedures. The composition and microstructure of samples was characterized by X-ray diffraction (XRD), raman and X-ray photoelectron spectroscopy (XPS). The morphology was observed under scanning electron microscope (SEM) and transmission electron microscopy (TEM) method. The specific surface area was analyzed using nitrogen adsorption/desorption test. The weight ratio of sulfur was measured through thermogravimetric (TG) analysis. The original structure and composition of biomass were improved by yeast fermentation and the auricularia auricula block structure with low porosity was modified. The electrochemical performance of NP-AC/S composite in Li-S batteries was systematically explored. After 100 cycles at 0.2C current density, there was still nearly 1000 mAh g −1 reversible capacity, holding a capacity retention rate of more than 84 %. The cycle performance and rate performance were both far better than the ordinary activated carbon materials.
研究领域:
材料科学
·
能源科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Hanliang Li,Lianzhu Lin,Mouming Zhao
This study aimed at recycling flavonoids with glycolipid lowering activity from Chenpi processing residue produced by essential oil extraction. The crude Chenpi flavonoids obtained by ethanol reflux mainly contained flavonone (hesperidin) and polymethoxyflavonoids (nobiletin, tangeretin, sinensetin, and isosinensetin), which complementally exerted strong glycolipid lowering activity as revealed by digestive enzyme inhibitory activity, molecular docking and network pharmacology. The reducing sugar and water-soluble impurities were efficiently removed by yeast fermentation and aqueous two-phase system (ATPS) extraction, respectively. The optimal combination use of yeast (2 % w / v , CN36) and ATPS formed by 22 % ( w /w) (NH 4 ) 2 SO 4 and 25 % (w/w) ethanol improved flavonoid purity to 60.25 %, enriched flavonone and polymethoxyflavonoids by 6.69-fold, and enhanced digestive enzyme inhibitory activity, which was superior to the use of individual yeast or ATPS, unoptimized combination, macroporous resin (XAD-16), and ethyl acetate. This study was the first to develop a recyclable biophysical method for simultaneous enrichment of flavonone and polymethoxyflavonoids.
研究领域:
药学
·
生物技术
·
食品科学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Ying Li,Hexiang Qiu,Zhiwei Sun,Lanhua Liu,Yousheng Wang,Dongdong Mu,Xuefeng Wu,Xingjiang Li
Honey tea wine is a kind of alcoholic drink made from green tea and honey by yeast. However, the mechanisms behind flavor variations caused by different microbial strains remain unclear. This study aims to compare the metabolic profiles of honey tea wine under single and mixed-culture fermentation using untargeted metabolomics. Results showed that mixed fermentation reduced ethanol content by 0.51% ( ν /v) but increased total phenols and flavonoids by 0.44 g/L and 0.34 g/L, respectively, compared to single fermentation. The mixed fermentation exhibited a greater diversity of volatile compounds and higher concentration levels compared to the single fermentation, with esters showing significantly elevated content. Differential metabolite analysis revealed that nucleotides, amino acids, sugars, and organic acids were key discriminators, linked to seven major metabolic pathways, including purine and pyruvate metabolism. Mixed fermentation significantly enhanced the content of bioactive compounds, including vitamins and protocatechuic acid. Our findings establish the metabolic framework and production protocol of honey wine tea, offering mechanistic insights to enhance tea and honey resource utilization via advanced processing strategies.
研究领域:
食品科学
·
微生物学
·
代谢组学
·
发酵技术
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Mengxiang Chen,Yi Peng,Rongtao Zhu,Xiaoting Luo,Xuemin Yang,Jiazhi Chen,Haiyan Chen,Wei Zhou,Zhiyun Du
Psoriasis is a chronic inflammatory skin disease with significant impacts on quality of life. Current treatments face challenges like high costs and side effects. This study explores the therapeutic potential of Rosa rugosa polysaccharide (RRPS) and its nanofiber membrane in psoriasis treatment. RRPS was extracted using a yeast fermentation enzyme-assisted method and characterized. In vitro studies on RAW264.7 macrophages showed that RRPS significantly reduced nitric oxide (NO) production, indicating anti-inflammatory effects. The nanofiber membrane, fabricated via electrospinning, has high porosity and sustained-release properties, enhancing drug delivery and skin barrier repair. In vivo studies using an imiquimod-induced psoriasis mouse model demonstrated that both RRPS and its nanofiber membrane improved psoriasis symptoms, reduced transepidermal water loss (TEWL), and inhibited pro-inflammatory cytokines (e.g., TNF-α, IL-6, IL-17A). Mechanistically, they suppressed the PI3K-AKT/mTOR pathway. The nanofiber membrane showed superior efficacy compared to RRPS alone, highlighting the benefits of electrospinning. This study suggests that Rosa rugosa polysaccharide and its nanofiber membrane could be a novel, effective, and safe alternative for psoriasis treatment by targeting the PI3K-AKT/mTOR pathway.
研究领域:
皮肤病学
·
药理学
·
纳米技术
·
免疫学
·
生物技术
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子