INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Chengyun Gan,Xiaoxue Yin,Yixuan He,Xiude Wu,Shuangjiu Qin,Wenping Ding,Xuedong Wang,Beibei Ding
Yeast polysaccharide has antioxidant, anti-tumor, antiviral, immune-enhancing, and adsorption properties; however, their utilization rate remains limited due to poor solubility. This study employed crude yeast polysaccharide (C-YP) as a raw material and utilized a 14 wt% NaOH aqueous solution as an environmentally friendly solvent to develop innovative yeast polysaccharide hydrogels (H-YPs). The prepared H-YPs exhibited a porous network structure with high water content. An elevation in yeast polysaccharide concentration corresponded with increased hardness and gel strength, reaching values of 665.0 gf and 155.0 g*cm, respectively. Furthermore, the H-YPs demonstrated non-cytotoxic characteristics, sustaining cell viability above 80 %. In vitro hypoglycemic assays indicated that H-YPs exhibited substantial inhibitory activity against the enzymes α-glucosidase and α-amylase, indicating their potential applicability in the development of healthy food gels.
研究领域:
食品科学
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生物化学
·
医学
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DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 18
·3188
·
IF 5.1
Rui Liao,Xiongjun Xiao,Huiling Huang,Kangjie Yu,Jianxia Tan,Yue Wang,Cong Li,Siyu Li,Yi Ma
This study investigated the potential of yeast polysaccharides (YPs) as an SO 2 substitute by adding varying concentrations (0–250 mg/L) prior to wine fermentation. The antioxidant activity and color stability were evaluated using UV–visible spectrophotometry, high-performance liquid chromatography, and colorimetric methods. Furthermore, HS-SPME-GC-MS and quantitative descriptive analysis were employed to compare the volatile compound profiles and sensory attributes between the optimal yeast polysaccharide treatment group (TS100, 100 mg/L) and the sulfur dioxide treatment group (S). Results showed that YPs addition effectively preserved phenolic compounds, with TS100 exhibiting the highest total phenols, flavonoids, and anthocyanins, where total anthocyanins reached 340.79 mg/L, a 2.52% increase over the S group. ABTS + scavenging activity showed no significant difference from the S group, confirming strong antioxidant capacity. Flavor compound content was higher in TS100 (10,458.99 μg/L) than in the S group (10,156.07 μg/L), with ethyl caprylate and ethyl decanoate being particularly prominent. These esters contributed intense fatty and fruity aromas, enhancing the overall sensory quality of the wine. In summary, 100 mg/L YPs addition yielded the best wine quality, demonstrating promising potential as an SO 2 alternative in winemaking.
研究领域:
食品科学
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酿酒学
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天然产物化学
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抗氧化研究
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风味化学
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食品质量与安全
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PubMed
高影响因子
INFLAMMATION
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2024
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IF 4.5
Liu Qi,Zhao Yubo,Dong Shuai,Bai Xingyuan,Chen Bin,Liu Xijuan,Shen Jing,Zhu Dan
Deciphering the complex and redundant process of acute inflammation remains challenging. The failure of numerous clinical trials assessing anti-inflammation agents which had promising preclinical effects inevitably questions the validity of current animal models of inflammation. This study aimed to better understand the process of immune inflammatory response and to select more suitable models to evaluate the effect of potential anti-inflammatory drugs. Zymosan and λ-carrageenan are the most used representatives of particulate and soluble irritants that trigger acute inflammation in the air pouch inflammation model. When zymosan was used, the number of exudate cells first increased at 4 h-8 h, followed by a drop at 12 h-24 h. While, the changes in number of leukocytes in peripheral blood and proportion of neutrophils in bone marrow have the opposite trend. Meanwhile, neutrophils released neutrophil extracellular traps (NETs) to clean zymosan particles. In contrast, the cell migration response to carrageenan increased during 4 h to 24 h, no obvious NETs were observed, and the number of leukocytes in peripheral blood increased and the proportion of neutrophils in bone marrow decreased slightly. This study indicated that although both zymosan and carrageenan are sterile irritants, the characteristics of the inflammatory response induced by each other were different. In the acute phase of inflammation, zymosan-stimulated neutrophils were mobilized, recruited, and engulfed, and then died by NETs. Carrageenan stimulated the production of cytokines/chemokines by neutrophils or macrophages, but did not lead to an obvious death by releasing NETs. Graphical Abstract
研究领域:
免疫学
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药理学
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炎症研究
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动物模型
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DOI
PubMed
高影响因子
BIOMACROMOLECULES
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2024
·
IF 5.5
Qingming Bao,Mingming Bao,Hai Xiao,Tsogzolmaa Ganbold,Shuqin Han,Huricha Baigude
The simultaneous delivery of CpG oligonucleotide along with short interfering RNA (siRNA) has the potential to significantly boost the anticancer impact of siRNA medications. Our previous research demonstrated that Curdlan nanoparticles functionalized with adenosine are capable of selectively delivering therapeutic siRNA to cancerous cells through endocytosis mediated by adenosine receptors. Herein, we synthesized a dual-ligand-functionalized Curdlan polymer (denoted by CuMAN) to simultaneously target tumor cells and tumor-associated macrophages (TAMs). CuMAN nanoparticles containing CpG and siRNA demonstrated enhanced uptake by B16F10 tumor cells and bone marrow-derived macrophages, which are facilitated by AR on tumor cells and mannose receptor on macrophages. This led to increased release of pro-inflammatory cytokines in both in vitro and in vivo settings. The synergistic effect of CpG on TAMs and RNAi on tumor cells mediated by the CuMAN nanoparticle not only suppressed the tumor growth but also strongly inhibited the lung metastasis. Our findings indicate that the CuMAN nanoparticle has potential as an effective dual-targeting delivery system for nucleic acid therapeutics.
研究领域:
生物医学工程
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肿瘤免疫学
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药物递送系统
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DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2019
·Vol. 36
·期 5
·774-780
·
IF 1.071
Cui Min,Ren Jujie,Wen Xiaofang,Li Na,Xing Yifei,Zhang Cong,Han Yuanyuan,Ji Xueping
Superoxide anion, one of the most active reactive oxygen species, is associated with the development of many diseases. So monitoring superoxide anion in living cells is of great significance for the pathological research of many diseases. In this work, a new non-enzymatic sensor for the detection of superoxide anion(O •− 2
) was developed, which was fabricated by the nanocomposites composed of manganese(III) tetraphenyl porphine(MnTPP) as superoxide dismutase mimic and electrochemical reduced graphene oxide(ERGO) as electrode support material to modify the glassy carbon electrode(GCE). The electrochemical behavior of the fabricated electrode(MnTPP/ERGO/GCE) was performed by electrochemical impedance spectroscopy(EIS) and cyclic voltammetry(CV), which revealed that MnTPP/ERGO/GCE possessed good catalytic ability to the electrochemical reduction of O •− 2
. The MnTPP/ERGO/GCE showed excellent electroanalysis performance towards O •− 2
using the technique of differential pulse voltammetry(DPV) with a linear relationship in the range of 0.2–110.0 µmol/L, a sensitivity of 445 µA·L·mmol −1 ·cm −2 and a detection limit of 0.039 µmol/L(S/N=3). The real-time monitoring of O •− 2
from MCF-7 breast cancer cells stimulated by zymosan was realized in this work, which indicates that the MnTPP/ERGO/GCE hold potential application for electrochemical quantification of superoxide anions in biological applications.
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高影响因子
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