INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2018
·Vol. 53
·期 9
·2205-2211
·
IF 2.383
Shengnan Shao,Xue Shen,Mingruo Guo
Summary Zinc-loaded whey protein nanoparticles were prepared by enzymatic cross-linking whey protein followed by ethanol desolvation. Whey protein isolate (WPI) was denatured by heating (80 °C for 15 min) at pH 7.0 and then cross-linked by transglutaminase at 50 °C for 4 h while stirring. Transglutaminase was inactivated by heating at 90 °C for 10 min, and then, ZnSO 4 ·7H 2 O (1–10 m m ) was added. Zinc-loaded whey protein nanoparticles were formed by adding ethanol at one to five times the volume of the protein solution at pH 9.0. The desolvated solutions were diluted by adding distilled water at ratio of 1:100 (w/v) immediately after desolvation. Dynamic light scattering (DLS) data showed that the particle size of zinc-loaded whey protein nanoparticles increased with the amount of zinc and the volume of ethanol. Scanning electron microscopy micrographs revealed an almost spherical morphology for zinc-loaded whey protein nanoparticles. The zinc loading efficiency was determined ranging from 76.7% to 99.2%. In vitro test data showed that the zinc release rate was low in simulated gastric fluid but high in simulated intestinal fluid. The results indicated that enzymatic cross-linked whey protein nanoparticles may be used as a good vehicle to deliver zinc as a supplement.
研究领域:
食品科学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
Polymer Chemistry
·
2014
·Vol. 5
·期 17
·5069-5076
·
IF 5.368
Kaixuan Ren,Chaoliang He,Yilong Cheng,Gao Li,Xuesi Chen
Enzymatically crosslinked injectable hydrogels based on poly(L-glutamic acid) grafted with tyramine and poly(ethylene glycol) (denoted as PLG-g-TA/PEG) were developed under physiological conditions in the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). Their gelation time, mechanical properties, swelling behaviors and porous structure were evaluated. The hydrogels were rapidly formed in the presence of low concentrations of HRP and H2O2. The storage modulus of the hydrogels could be well controlled and increased by increasing the concentrations of HRP and H2O2. The average pore-size of the hydrogels varied from 20 to 120 μm, depending on the H2O2 concentration. In addition, the encapsulated L929 fibroblast cells in the PLG-g-TA/PEG hydrogels exhibited high viability. After subcutaneous injection of the PLG-g-TA/PEG solutions containing HRP and H2O2 into the back of rats, the hydrogels were rapidly formed in situ. The hydrogels were found to persist for up to 10 weeks in vivo, and histological analysis indicated that the hydrogels exhibited acceptable biocompatibility. These results suggested that the biocompatible, injectable enzyme-mediated PLG-g-TA/PEG hydrogels are promising for biomedical applications including tissue engineering scaffolds and drug delivery carriers.
研究领域:
生物材料
·
组织工程
·
药物递送
✓ 已验证
DOI
高影响因子
Polymer Chemistry
·
2017
·Vol. 8
·期 45
·7017-7024
·
IF 5.375
Kaixuan Ren,Bin Li,Qinghua Xu,Chunsheng Xiao,Chaoliang He,Gao Li,Xuesi Chen
In the present work, a horseradish peroxidase (HRP)-catalyzed hydrogel based on a double-end tyramine conjugated linear poly(ethylene glycol) (TA-PEG-TA) polymer is developed. The TA-PEG-TA is designed to act as not only a substrate of HRP, but also a macromonomer. We find that, the linear structure is sufficient to form robust hydrogels, and therefore it greatly simplifies the polymer architecture applicable to HRP-catalyzed hydrogels, which are always based on graft or branched polymers. The gelation time and strength of the TA-PEG-TA hydrogels can be well tuned by varying the concentrations of the polymer and HRP as well as the molar ratios of H2O2 to TA groups. The gelation mechanism is clarified by exploring the HRP-catalyzed reaction of a single-end tyramine conjugated poly(ethylene glycol) monomethyl ether (mPEG-TA). The multiple active hydrogen atoms (in the hydroxyl group and the o-position) of phenol moieties can be oxidized by H2O2via HRP catalysis, which should be responsible for the generation of mPEG-TA oligomers and the gelation of TA-PEG-TA. This study provides a new strategy for the design of injectable enzymatically crosslinked hydrogels.
研究领域:
生物材料
·
高分子化学
·
组织工程
✓ 已验证
DOI
高影响因子
Science China-Chemistry
·
2013
·Vol. 56
·期 12
·1701-1709
·
IF 1.327
Chen ZhiPing,Wang Wei,Guo Lei,Yu YanYan,Yuan Zhi
For the requirement of preliminary vascularization, the scaffolds for thick tissue engineering should possess not only good cell affinity, but also anticoagulant ability. In this paper, an enzymatically crosslinked hydrogel scaffold based on sulfated chitosan (SCTS) was prepared. Firstly, sulfated chitosan-hydroxyphenylpionic acid (SCTS-HPA) conjugate was synthesized, and its structure was identified by FITR and 1 H NMR. And then an enzymatically crosslinked hydrogel was prepared in the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H 2 O 2 ). The gelation time, mechanical property, morphology and cytotoxicity to human umbilical vein endothelial cells (HUVECs) of the hydrogel was evaluated in vitro , the tissue compatibility of SCTS scaffold was studied in vivo . The results showed that the gelation time, mechanical property, morphology of the dehydrated hydrogel could be controlled by the HRP and H 2 O 2 concentration. The cytotoxicity test showed that the hydrogel extracts had no cytotoxicity to HUVECs. The in vivo assay indicated that SCTS-HPA scaffold showed good tissue compatibility, and no thrombus formation. All these results indicated that the SCTS-HPA scaffold could be used as thick tissue engineering scaffold.
研究领域:
生物材料学
·
组织工程学
·
再生医学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2018
·
IF 3.997
Bo Bi,Hui Liu,Wenting Kang,Renxi Zhuo,Xulin Jiang
The in-situ forming injectable hydrogels have received much attention as scaffolds in the biomedical field, providing a minimally invasive surgical procedure to fill the damaged area. In the present work, carboxymethyl chitin (CMCH) synthesized homogenously was further functionalized with tyramine , resulted in a new injectable enzymatically crosslinked in-situ forming hydrogel under physiological conditions. This new tyramine-modified carboxymethyl chitin (CMCH-Tyr) hydrogel showed much better mechanical properties than those of the thermosensitive in-situ forming physical-crosslinking CMCH hydrogel. The CMCH-Tyr hydrogels remained stable under physiological conditions and could be degraded by lysozyme. The gelation time, strength and biodegradation rate of the CMCH-Tyr hydrogels can be adjusted by varying the concentrations of the horseradish peroxidase and H 2 O 2 in the certain range. In vitro cytotoxicity assays and in vivo in-situ injection study showed non-toxicity, favorable gel formation, and good tissue biocompatibility of the enzyme-catalyzed CMCH-Tyr hydrogel. Thus, the biodegradable and biocompatible CMCH-Tyr hydrogels may hold great potential for three dimensional cell culture and tissue engineering.
研究领域:
生物医学工程
·
组织工程
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Lin Wang,Bo Xu,Yelin Nong,Ping Wang,Yuanyuan Yu,Chao Deng,Jiugang Yuan,Qiang Wang
Silk fibroin (SF) is a promising biomaterial with attractive properties, however, the practical applications of pure regenerated SF materials were severely restricted by their poor water retention and low elasticity. In this study, a tyramine-modified hyaluronic acid (mHA) was introduced to SF chains, followed by constructing double-network hydrogels via laccase-mediated crosslinking of SF and mHA. Mechanism of the enzymatic coupling reaction between SF and mHA was investigated using the model compounds. The morphology, mechanical property, and swelling behavior of SF-g-mHA hydrogel were evaluated. The results reveal that combination use of SF and mHA endowed the obtained hydrogels with high structural stability, improved mechanical properties and drug release behavior, which overcame the intrinsically poor formability of the pure fibroin hydrogels. This work provides a novel method to construct a double-network hydrogel with high flexibility and toughness, and expands the application range of the SF-based materials in biomedical fields.
研究领域:
生物材料学
·
生物医药工程
✓ 已验证
DOI
高影响因子
Gels
·
2025
·Vol. 11
·期 2
·93
·
IF 5.3
Wenzhuo Hou,Hui Yi,Guangyan Zhang
In recent years, hydrogel beads and in situ hydrogels have gained wide attention in various fields such as biomedicine. In this study, 3-(4-hydroxyphenyl) propionic acid (HP) was introduced into the side chain of poly(α,β-[N-(2-hydroxyethyl)-D,L-aspartamide]) (PHEA) to synthesize phenolic hydroxyl-functionalized poly(aspartamide) derivative PHEA-HP with enzyme-catalyzed cross-linking potential. First, the chemical structure of PHEA-HP was characterized by FT-IR, UV and1H NMR, and the results of in vitro cytotoxicity against L929 cell line and hemolysis experiment showed that PHEA-HP did not have toxicity to cells (viability > 90%) and had good blood compatibility. Then, rheological measurement confirmed the formation of PHEA-HP-based in situ hydrogel with a high storage modulus (G′) around 104Pa, and the vial-tilting method revealed that the gelation time of PHEA-HP aqueous solution could be tuned in the wide range of 5–260 s by varying the concentrations of hydrogen peroxide (H2O2) and horseradish peroxidase (HRP). Finally, hydrogel beads of different diameters containing methylene blue (for easy observation) were prepared using a coaxial needle and syringe pumps, and the effect of the flow rate of the outer phase on the diameters of the hydrogel beads was also investigated. Therefore, PHEA-HP may be a promising and safe poly(aspartamide) derivative that can be used to prepare in situ hydrogels and hydrogel beads for applications closely related to the human body.
研究领域:
生物材料学
·
生物医药工程
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 5
·2870-2876
·
IF 3.119
Luyu Wang,Jinrui Li,Dan Zhang,Shanshan Ma,Junni Zhang,Feng Gao,Fangxia Guan,Minghao Yao
Recently, in situ formed injectable hydrogels have shown great potential in biomedical applications as therapeutic implants or carriers in tissue repair and regeneration. They can seal or fill the damaged tissue to function as cell/drug delivery vehicle perfectly through a minimally invasive surgical procedure. In this study, hyaluronic acid (HA) is functionalized with tyramine to produce an injectable hydrogel dual-enzymatically crosslinked by horseradish peroxidase (HRP) and galactose oxidase (GalOX). This new tyramine-modified HA (HT) hydrogel exhibited good injectability, favorable cytocompatibility to mice bone marrow mesenchymal stem cells (BMSCs), and low inflammatory response verified by cytotoxicity assay in vitro and an in situ subcutaneous injection study in vivo. In addition, the gelation time, swelling behavior, and degradation rate of the HT hydrogel could be adjusted through varying the concentrations of HT and GalOX in a certain range. These encouraging results suggest that such biocompatible HT hydrogels might have potential application in three-dimensional stem cell culture and tissue engineering.
研究领域:
生物材料
·
再生医学
·
细胞生物学
✓ 已验证
DOI
高影响因子
Polymers
·
2021
·Vol. 13
·期 20
·3565
·
IF 4.329
Qian Tao,Julong Zhong,Rui Wang,Yuzhu Huang
Both ionic and enzymatic crosslink are efficient strategies for constructing network materials of high biocompatibility. Here chitosan was modified firstly and then crosslinked by these two methods for complementary advantages. The preparation methods and ionic crosslinkers can regulate the size and uniformity of the multiple-crosslinked nanogels. The multiple-crosslinked nanogels with the smallest size and the best uniformity was selected for the drug delivery. The drug-loading content and encapsulation efficiency were up to 35.01 and 66.82%, respectively. Their release behaviours are correlated with the pH value and the drug dosage. In general, the lower pH value and the lower drug dosage promoted the drug release. With the assistance of several kinetic models, it is found that drug diffusion plays a preponderant role in drug release, while polymer relaxation has a subtle effect. The multiple-crosslink resulting from ionic compounds and enzymes may provide a new perspective on developing novel biocompatible materials.Keywords:multiple-crosslinked nanogel;ionic crosslink;enzymatic crosslink;drug delivery;natural polymeric materials
研究领域:
生物材料学
·
药物递送系统
·
高分子化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomedical Materials
·
2022
·Vol. 18
·期 1
·015006
·
IF 4.103
Peng Ding,Qianqian Wei,Ning Tian,Xiaoyue Ding,Ling Wang,Bin Wang,Oseweuba Valentine Okoro,Amin Shavandi,Lei Nie
The enzymatically crosslinked hydrogel could replicate the cellular microenvironment for biomedical applications. In the present study, to improve the cytocompatibility of chitosan (CS), sialic acid (SA) was introduced to CS to synthesize sialylated CS (CS-SA), and the tyramine (TA) was grafted to gelatin (G) to obtain TA modified gelatin (G-TA). The successful synthesis of CS-SA and G-TA was confirmed using 1H NMR and UV–Vis absorption spectra. The interpenetrating polymer networks G-TA/CS-SA (GC) hydrogel was then fabricated via blending G-TA and CS-SA solutions and crosslinked using horseradish peroxidase. The storage modulus (G') of the fabricated GC hydrogels with different ratios of G-TA/CS-SA greatly varied during the formation and strain of hydrogels. With the increase of CS-SA concentration from 0% to 2%, the storage modulus of GC hydrogels was also observed to decrease from 1500 Pa to 101 Pa; the water uptake capacity of GC hydrogels increased from 1000% to 4500%. Additionally, the cell counting kit-8 and fluorescent images demonstrated the excellent cytocompatibility of GC hydrogels after culturing with NIH 3T3 cells. The obtained results indicated that the fabricated GC hydrogels might have potential in biomedical fields, such as wound dressing.
研究领域:
生物材料学
·
组织工程学
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2021
·
IF 4.749
Zhenjie Zhang,Xiaoyan Niu,Xiaoyue Feng,Xiaohui Wang,Licheng Yu,Wei Wang,Zhi Yuan
The method of tumor microenvironment (TME)-responsive aggregation has become a promising approach to enhance treatment effect by improving the accumulation of nanoparticles in tumors. The enzymatic cross-linking strategy has widely attracted attention owing to its good aggregation stability and biocompatibility. However, the enzymes in nontumor tissue can also catalyze the cross-linking reaction and reduce accumulation of nanoparticles in tumor. In this work, a “dual key”-responsive strategy is utilized to construct a transglutaminase (TGase)/pH-responsive radiosensitizer (Au@TAcoGal) with specific aggregation behavior in hepatic tumor cells. Au@TAcoGal can retain its stability in blood circulation (pH 7.4) even in the presence of TGase in plasma. On reaching tumor sites, it can be endocytosed by hepatoma cells by the active targeting of phenylboronic acid (PBA) and aggregated under acidity and overexpression of TGase in cells. Due to its specific accumulation in hepatoma cells, radiotherapy can be operated under a lower dose of X-ray. The results show that the cellular accumulation of Au@TAcoGal increases by 30–70%, and the cell survival rate is less than 25% under X-ray irradiation. The antineoplastic results show that Au@TAcoGal exhibits a higher therapeutic effect, and the tumor inhibition rate can reach 84.21%.
研究领域:
纳米技术
·
癌症研究
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Qiaozhi Zhang,Zhouzhou Cheng,Yong Wang,Shuyao Zheng,Yanbo Wang,Linglin Fu
Alcalase hydrolysis of soybean proteins was shown effective in degrading epitopes, but with a significant loss of tastes and functional properties. In this study, the combined use of controlled Alcalase hydrolysis and transglutaminase (TGase)-cross-linking was used to produce hypoallergenic soybean protein hydrolysates (SPHs) with improved flavor and techno-functional properties. Through establishing the kinetic model of heterogeneous enzymatic processes, SPHs with different degrees of hydrolysis (2.5–10.0%) were obtained and polymerized by TGase to yield products with various peptide components. Results showed that post-hydrolysis cross-linking rearranged the (poly)peptides in SPHs via glutaminyl modifications. Following TGase-treatments, due to the masking of bitter-active peptides and free amino acids , the bitterness of SPHs was significantly reduced with a slight improvement in the overall flavor. Furthermore, TGase–cross-linking rescued multiple techno-functionalities of SPHs, particularly their emulsifying and foaming properties. Using sera from soybean-allergic individuals, it was evidenced that TGase-addition did not generate neoallergens and the residual allergenicity of SPHs was not distinctly affected. The final products maintained 44–71% reduction in the IgE-binding levels in comparison to the unhydrolyzed protein. Combining limited Alcalase hydrolysis with TGase–cross-linking provides a feasible way to produce hypoallergenic soybean ingredients with desired flavor and functional attributes.
研究领域:
食品科学
·
蛋白质化学
·
过敏研究
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）