ACS Applied Materials & Interfaces
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2022
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IF 10.383
Qi Zeng,Mengmeng Sun,Xin Xie,Yuli Zhang,Haoyue Hou,Xingyuan Fang,Ting Guo,Hao Yuan,Tao Meng
Pickering interfacial biocatalysis (PIB) paves the way for efficient enzymatic catalysis in the biphasic system. However, the Pickering interfacial biocatalysts located on the oil–water interface still face the inevitable deactivation when one of the phases contains the reactant that inactivates the enzyme. Herein, the positioning of lipase-entrapped colloidosomes (LECs) at the emulsion interface is rationally designed by physically tuning the wettability, which allows LECs to protrude into the selected phase to protect the lipase away from the damage of the reactant. As a proof of concept, LECs with different positioning at the interface are used as Pickering interfacial biocatalysts to produce biodiesel by esterification of lauric acid and methanol. Impressively, the LECs that protrude into the oil phase possess an optimal catalytic performance to protect more lipases away from the damage of the reactant of short-chain alcohol, which shows an 8.18-fold enhancement in specific activity relative to the free lipase, reach a biodiesel yield of 80.37% after 8 h, and retain the 96.44% of relative activity after 10 cycles. This study provides a novel and robust platform for Pickering emulsion-enhanced biocatalysis.
研究领域:
生物化学
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材料科学
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催化工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
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2014
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IF 8.312
Juan Zhang,Zhen Wang,Weifeng Lin,Shengfu Chen
Safety and high efficacy of vectors are essential requirements for gene therapy. To address these challenges, poly(carboxy betaine methacrylate ethyl ester)-poly(carboxy betaine methacrylate) (PCBMAEE-PCBMA) diblock copolymers were synthesized to form core–shell vector for gene delivery. The hydrophobic PCBMAEE segment, a polyzwitterionic precursor, can condense plasmid DNA (pDNA) into a hydrophobic core, which improves pDNA protection from nuclease attack and maintains the condensed structure against dilution. Moreover, the hydrolysis of PCBMAEE in uptaken gene vectors can enhance the pDNA release and reduce the cytotoxicity caused by the cationic polymer accumulation in the host cells. The PCBMA segment, zwitterionic fouling resistant material , is utilized to stabilize the gene vector in the complex medium and reduce the interference from serum proteins without impeding the endocytosis of DNA vector like PEG protection layer. Results showed that the complex formed by PCBMAEE 50 -PCBMA 14 with luciferase or pEGFP gene exhibit higher transfection efficacy of pDNA than that formed by PEI 25 kDa or Lipofectamine ® 2000 in tested cell lines (COS-7, HepG-2, HeLa, and HUVEC), especially, in difficult-to-transfect ones, such as HeLa and HUVEC. The luciferase expression level infected by the vectors of PCBMAEE 50 -PCBMA 14 /pGL-4 at N/ P = 20/1 is 27 times of the branched PEI 25 kDa in COS-7 cells and 16 times of Lipofectamine ® 2000 in HUVEC. Furthermore, the complex formed by PCBMAEE 50 -PCBMA 14 also show advantages in transfection rate, dosage effectiveness and preservation of transfecting activity in serum contained growth medium. The luciferase expression of the vectors of PCBMAEE 50 -PCBMA 14 /pGL-4 at N/ P = 20/1 is 230 times higher than that of PEI complex at low vector dosage (the 5% standard dosage). And the transfection rate is 25 times higher than that of PEI complex in 10% serum contained growth medium. In short, all these results indicated that the polymeric gene vector, consisted of convertible hydrophobic polyzwitterionic precursor and fixed polyzwitterionic fouling resistant segment, is a promising candidate for high and stable gene transfection in complex growth medium.
研究领域:
分子生物学
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基因治疗
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生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Physical Chemistry C
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2016
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IF 4.509
Mufang Li,Mengying Luo,Fei Li,Wenwen Wang,Ke Liu,Qiongzhen Liu,Yuedan Wang,Zhentan Lu,Dong Wang
Copper-based inorganic–protein hybrid nanoflowers were constructed on the surface of a nanofiber membrane, creating a highly biocompatible, biomimetic, and multilevel surface. The inorganic–enzyme nanoflowers exhibited enhanced durability and stability arising in part from the protection of the enzyme by the inorganic crystals. The high specific surface area and abundant functional groups of Cu2+ on the nanofiber membrane enable the dense and compact growth of Cu3(PO4)2·3H2O–protein nanoflowers. The structure, morphology, and crystallization of the copper-based inorganic–protein nanoflowers were analyzed, which led us to propose a formation mechanism of the hybrid nanoflower. The composition and structure of the copper-based inorganic–protein nanoflowers on the nanofiber membrane could be controlled by varying the structure of the nanofiber membrane, formulation of mineralizing solutions, concentration of protein, and growing time. Different structures of protein, such as BSA, papain, laccase, and HRP, show different morphologies. The construction of copper-based inorganic–protein nanoflowers on nanoscale fibrous membrane opens new possibilities for preparing biomimetic multilevel structured materials and makes them an ideal material for biosensor, biocatalysis, bioengineering, and biodevices.
研究领域:
材料科学
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生物化学
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生物工程
✓ 已验证
产品关联
DOI
高影响因子
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