To investigate the impact of each functional group on the emulsifying properties of sugar beet pulp pectin (SBPP), seven enzymes were studied in a particular order. Compositions of SBPP and enzymatically modified SBPPs were determined, and the structures of SBPPs were characterized by FT-IR. In addition, the contribution of each functional group was evaluated based on the variation in emulsion characteristics. The results showed that protein, ferulic acid-araban/galactan-protein complexes and ferulic acid played important roles in improving the surface activity, emulsifying capacity and emulsifying stability of SBPP and the extent of the decrease in the emulsifying activity followed the order: ferulic acid > ferulic acid-arabinogalactan-protein complexes > protein. The decrease of methyl ester groups mainly affected the particle sizes of the emulsion. In addition to particle sizes, the cream index of the emulsion increased with the hydrolysis of acetyl groups. Arabinose and galactose less affected emulsifying properties than other functional groups.
Background Laminaria japonica polysaccharide (LJP), a fucose enriched sulfated polysaccharide has been demonstrated to have excellent anticoagulant and antithrombotic activities. However, the antithrombotic effect of low molecular weight polysaccharide from enzymatically modified of LJP (LMWEP) remains unknown. Material/Methods LMWEP was prepared by fucoidanase enzymatic hydrolysis, and the antithrombotic and anticoagulant activities, and the underlying mechanism were investigated thoroughly. Rats were randomly divided into 6 groups (8 rats in each group): the blank control group, the blank control group treated with LMWEP (20 mg/kg), the model group, the model group treated with heparin (2 mg/kg), the model group treated with LJP (20 mg/kg), and the model group treated with LMWEP (20 mg/kg). After 7 days of intravenous administration, blood was collected for biochemical parameters examinations. Results LMWEP increased the activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), 6-keto prostaglandin F1α (6-Keto-PGF1α), and endothelial nitric oxide synthase (eNOS). In addition, LMWEP decreased fibrinogen (FIB), endothelin-1 (ET-1), thromboxane B2 (TXB2), erythrocyte sedimentation rate (ESR), and hematocrit (HCT). Conclusions LMWEP, an enzymatically modified fragment with a molecular weight of 25.8 kDa, is a potential antithrombotic candidate for treatment of thrombosis related diseases.
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