TALANTA
·
2023
·
IF 6.1
Chengjie Duan,Yanheng Yao,Wenting Cheng,Yan Chen,Jin Jiao,Yang Xiang
Aptazyme is a chimera of functional nucleic acids , which integrates recognition and amplification elements to simplify the assay process and improve sensing efficiency. However, its application may be limited by signal leakage. In this work, we innovatively integrate the AβO aptamer and an MNAzyme (multicomponent nucleic acid enzyme) for highly efficient detection of AβO. The aptamer and half of the MNAzyme are positioned at one strand, and the other half of the MNAzyme is integrated with a toehold sequence. These two sequences cannot hybridize to activate the MNAzyme until the target is added. The background signal is significantly reduced by the split format and the secondary structure of DNA probes formed in the absence of the target. The proposed aptazyme can not only achieve amplification through enzymatic catalysis but also greatly improve the efficiency of signal transduction and output. We systematically investigated the influence of different DNA probes on the detection performance, and the optimized aptazyme can detect as low as 26.5 pM targets in 1h. The stability of this method was also investigated by detection targets in real biological samples.
研究领域:
生物化学
·
分子生物学
·
神经科学
✓ 已验证
产品关联
DOI
高影响因子
BIOMACROMOLECULES
·
2021
·
IF 6.988
Yu Zhang,Bo Xia,Yanyan Li,Xianfu Lin,Qi Wu
The synthesis of optically pure polymers is one of the most challenging tasks in polymer chemistry. Herein, Novozym 435 (Lipase B from Candida antarctica, immobilized on Lewatit VP OC 1600)-catalyzed polycondensation between d-/l-aspartic acid (Asp) diester and diols for the preparation of helical chiral polyesters was reported. Compared with d-Asp diesters, the fast-reacting l-Asp diesters easily reacted with diols to provide a series of chiral polyesters containing N-substitutional l-Asp repeating units. Besides amino acid configuration, N-substituent side chains and the chain length of diols were also investigated and optimized. It was found that bulky acyl N-substitutional groups like N-Boc and N-Cbz were more favorable for this polymerization than small ones probably due to competitively binding of these small acyl groups into the active site of Novozym 435. The highest molecular weight can reach up to 39.5 × 103 g/mol (Mw,Đ = 1.64). Moreover, the slow-reacting d-Asp diesters were also successfully polymerized by modifying the substrate structure to create a “nonchiral” condensation environment artificially. These enantiocomplementary chiral polyesters are thermally stable and have specific helical structures, which was confirmed by circular dichroism (CD) spectra, scanning electron microscope (SEM), and molecular calculation.
研究领域:
高分子化学
·
生物催化
·
材料科学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Xiaotong Cao,Jianquan Luo,John M. Woodley,Yinhua Wan
Micropollutants present in water have many detrimental effects on the ecosystem. Membrane technology plays an important role in the removal of micropollutants, but there remain significant challenges such as concentration polarization, membrane fouling, and variable permeate quality. The work reported here uses a multifunctional membrane with rejection, adsorption, and catalysis functions to solve these problems. On the basis of mussel-inspired chemistry and biological membrane properties, a multifunctional membrane was prepared by applying “reverse filtration” of a laccase solution and subsequent “dopamine coating” on a nanofiltration (NF) membrane support, which was tested on bisphenol A (BPA) removal. Three NF membranes were chosen for the preparation of the multifunctional membranes on the basis of the membrane properties and enzyme immobilization efficiency. Compared with the pristine membrane, the multifunctional membrane exhibited significant improvement of BPA removal (78.21 ± 1.95%, 84.27 ± 7.30%, and 97.04 ± 0.33% for NT103, NF270, and NF90, respectively), all of which are clearly superior to the conventional Fenton treatment (55.0%) under similar conditions and comparable to soluble laccase coupled with NF270 membrane filtration (89.0%). The improvement would appear to be due to a combination of separation (reducing the enzymatic burden), adsorption (enriching the substrate concentration as well as prolonging the residence time), and lastly, catalysis (oxidizing the pollutants and breaking the “adsorption saturation limits”). Furthermore, the synergistic effect of the polydopamine (PDA) layer on the enzymatic oxidation of BPA was confirmed, which was due to its enhanced adsorption and electron transfer performance. The multifunctional membrane could be reused for at least seven cycles with an acceptable activity loss, demonstrating good potential for removal of micropollutants.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
Guang-Li Wang,Xue-Lian Hu,Xiu-Ming Wu,Zai-Jun Li
In this paper, a novel and sensitive assembled quantum dots (QDs)–bienzyme (glucose oxidase (GOD) and horseradish peroxidise (HRP)) hybrid system was designed for the direct determination of glucose. Using o-phenylenediamine (OPD) or 3,3′-diaminobenzidine (DAB) as substrate of HRP, whose corresponding oxidation product (oxOPD or oxDAB) effectively quenched the fluorescence of thioglycollic acid (TGA)-capped CdS QDs. The fluorescence quenching of the CdS QDs was proportional to the concentration of glucose and a detection limit of 0.1 μmol/L (or 20 nmol/L) was achieved under the optimum conditions when using OPD (or DAB) as the substrate of HRP. The sensing system was also applied to the determination of glucose in human serum with satisfactory results without any need for complicated sample pretreatments. Other sugars such as fructose, sucrose and lactose hardly interfered on the detection of glucose. The developed method manifested several advantages of high sensitivity, short analysis time, low cost and easy of operation. Moreover, it had great potential in the direct detection of real samples in clinical diagnostics of various diseases.
研究领域:
生物化学
·
医学诊断
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Molecular Catalysis
·
2025
·
IF 4.9
Jiali Chen,Chen Huang,Yuqing Zhang,Liya Zhou,Li Ma,Lihui Wang,Yanjun Jiang,Yunting Liu
The conversion of racemic alcohols to enantiopure alcohols and amines is of significant importance yet presents considerable challenges. Herein, we achieved this goal by developing continuous-flow chemoenzymatic systems. In chemical module, a ruthenium (II)-N-heterocyclic carbene (Ru-NHC) catalyst immobilized on dendritic organosilica nanoparticle (DON) was prepared, which exhibited high activity and selectivity in the dehydrogenative oxidation of racemic alcohols to ketones. In enzymatic module, a ketoreductase and an amine dehydrogenase were individually co-immobilized with a cofactor-regenerating enzyme (glucose dehydrogenase) on DON for the enantioselective conversion of ketones to chiral alcohols and amines, respectively. The integration of the two modules in continuous-flow systems enabled the efficient chemoenzymatic deracemization and asymmetric amination of racemic alcohols (9 examples) with the maximum space-time yields of 6.8 and 5.8 g L -1 h -1 , respectively, underscoring the practical utility of the continuous-flow chemoenzymatic systems.
研究领域:
生物化学
·
有机化学
·
合成生物学
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Zhe Dong,Yu Zhao,Jinliang Chen,Ming Chang,Xiaosan Wang,Qingzhe Jin,Xingguo Wang
Borneol esters have attracted increasing interest due to their potential application in the food industry. In this study, the extraction of d -borneol from Cinnamomum camphora chvar. borneol seed was carried out via steam distillation to gain highly purified d -borneol (99.66%). The lipase-catalyzed lipophilization of extracted d -borneol with oleic acid was performed in a solvent-free system. The influences of lipase type, substrate molar ratio, lipase loading, reaction temperature and reaction time on d -bornyl oleate yield were evaluated. Among four kinds of commercial lipases screened for their esterification efficiency, CRL (lipase from Candida rugose ) displayed the highest catalytic activity in the synthesis of d -bornyl oleate. The yield of more than 95% was obtained under the optimal conditions: substrate molar ratio 1:2 ( d -borneol/oleic acid), lipase loading 8%, reaction temperature 45 °C, reaction time 48 h. The structural identification of d -borneol and product d -bornyl oleate was performed by gas chromatography mass spectrometry (GC-MS) and proton nuclear magnetic resonance spectroscopy ( 1 H NMR). The results showed that the product was confirmed to be d -bornyl oleate and the purity of which was 97.51%. This study will pave the way for d -borneol and its esters application in food industry.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2019
·
IF 3.372
Huan Xia,Zhixian Li,Xue Zhong,Binghui Li,Yunpeng Jiang,Yanbin Jiang
The combination of enzymatic and chemical reactions to achieve co-operative chemo-enzymatic processes has presented many challenges for decades. In this work, alcohol dehydrogenase (ADH) was encapsulated into metal-organic framework HKUST-1 crystals through an in situ approach to construct a synergistic chemo- and bio-catalysis system, where ADH was for the conversion of benzyl alcohol to benzaldehyde, and HKUST-1 was in charge for the regeneration of β-nicotinamide adenine dinucleotide (NAD + , the oxidized cofactor). It was found that HKUST-1 was a versatile catalyst for efficient oxidation of NADH in different buffers. The Michaelis-Menten kinetic parameters of immobilized ADH exhibited an increased K M (approximately 1.3-fold) and a decreased V max (approximately 4.7-fold) compared to free ADH. Due to the protection of HKUST-1, the immobilized enzyme showed a better resistance against urea and organic solvents compared with free enzyme, and still maintained 93% of its original activity after 6 cycles. The immobilized enzyme was successfully applied to the enzymatic transformation of benzyl alcohol to benzaldehyde in aqueous media at room temperature. Owing to the oxidized cofactor regeneration by HKUST-1, ADH@HKUST-1 composites achieved a benzaldehyde yield of 28.5%, which was approximately 4.3-fold higher than that of the individual bio-catalysis. This concept of combining chemo- and bio-catalysis may provide a novel and versatile technique for applications in biochemical catalysis, biosensors and drug delivery.
研究领域:
生物化学
·
催化科学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2015
·
IF 4.074
Yuanyuan Yu,Qiang Wang,Jiugang Yuan,Xuerong Fan,Ping Wang,Li Cui
Hydrophobic cotton fabrics were prepared by grafting octadecylamine (ODA) onto cotton fiber surfaces via the laccase/2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) treatment. The cotton fibers were oxidized by laccase/TEMPO to introduce aldehyde groups, which reacted with the amino groups of ODA to form Schiff base. First, ODA was coupled to glucan, used as a model compound of cellulose. The results of FT-IR and MALDI-TOF mass spectroscopy prove the formation of a Schiff base between ODA and glucan. Moreover, the existence of ODA in the grafted cotton fibers was verified by ATR-FTIR, elemental analysis, X-ray photoelectron spectroscopy. Finally, the hydrophobicity of the ODA-grafted cotton fabrics was estimated. The surface hydrophobicity of the cotton fabrics increased after the enzymatic grafting reaction.
研究领域:
材料科学
·
纺织工程
·
生物化学
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产品关联
DOI
PubMed
高影响因子
CATALYSIS LETTERS
·
2021
·Vol. 152
·期 4
·1215-1223
·
IF 3.186
Wang Yun,Cheng Hong,He Jia-Rui,Yao Qiao-Xia,Li Li-Ling,Liang Zhi-Hui,Li Xiang
The Knoevenagel condensation of acetylacetone and aromatic aldehyde catalyzed by lipase from porcine pancreas(PPL) and α-amylase from hog pancreas in ionic liquids and deep eutectic solvents was achieved for the first time. The highest yields is up to 96.1%. Mild reaction conditions, enhanced rates, improved yields and reagents’ reactivity which is different from that in standard organic solvents are the remarkable features observed in ionic liquids and deep eutectic solvents. Graphical Abstract
研究领域:
生物化学
·
有机化学
✓ 已验证
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Wenyan Wang,Yuanyuan Yu,Ping Wang,Qiang Wang,Yongqiang Li,Jiugang Yuan,Xuerong Fan
This study reports on eco-friendly graft polymerization approach for the modification of a cellulosic material via combination between enzymatic catalysis and reversible addition-fragmentation chain transfer polymerization (RAFT). Polyacrylamide (PAM) was polymerized on a cellulosic filter paper via horseradish peroxidase (HRP)-initiated RAFT polymerization. The results of grafting ratio, conversion, and pseudo-first-order kinetics were proved that the PAM graft polymerization on the filter paper followed RAFT rules. The results of Attenuated total reflection (ATR-FTIR), elemental analysis, and X-ray photoelectron spectroscopy (XPS) confirmed the presence of PAM in PAM-grafted filter paper. The results of water contact angle and Thermogravimetric analysis (TG) evidenced the change in the wetting properties and thermal performance, respectively of the treated filter paper. This work provides a new environmentally approach to graft polymerization on cellulosic materials.
研究领域:
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Zhiyu Xue,De Ning,Kaihong Jia,Hao Liu,Yong Xiang,Jinlong Cao,Junxian Chen,Yeshuang Zhong,Xinyu Wang,Zhen Zhang
Carbon dots (CQD) have received significant attention as a novel ratiometric fluorescent pH nanoprobe, owing to their favorable optical properties and excellent biocompatibility. Despite their appealing features, the precise mechanism behind the pH-sensitive photoluminescence of CQDs remains to be fully understood. This study endeavors to unravel the mechanism underlying the pH-responsive ratiometric fluorescence in dual-emission CQDs, synthesized through a one-step hydrothermal method using o-phenylenediamine and oxalic acid as precursors. The resultant CQDs exhibit inherent dual-emission at wavelengths of 383 nm and 566 nm, with the ratiometric fluorescence response tailored by the ratio of precursors, providing a robust tool for pH sensing across a range of 2 to 6. Detailed characterizations, including chemical, morphological, and optical analyses, alongside theoretical insights from time-dependent density functional theory (TD-DFT), elucidate the mechanism underlying the pH-dependent luminescence, attributed to the electron cloud transmission between amide and adjacent carboxyl groups on the CQD surface. The superior performance of these CQDs in real-time pH monitoring is demonstrated through their application in glucose oxidase-catalyzed reactions, showcasing their potential as efficient, reliable nanoprobes for biomedical research and diagnostic applications.
研究领域:
材料科学
·
生物医学工程
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Catalysts
·
2020
·Vol. 10
·期 9
·1057
·
IF 3.52
Jing Wang,Sheng Xu,Yang Pang,Xin Wang,Kequan Chen,Pingkai Ouyang
To achieve efficient bio-production of phospholipase D (PLD), PLDs from different organisms were expressed inE.coli. An efficient secretory expression system was thereby developed for PLD. First, PLDs fromStreptomycesPMF andStreptomyces racemochromogeneswere separately over-expressed inE.colito compare their transphosphatidylation activity based on the synthesis of phosphatidylserine (PS), and PLDPMFwas determined to have higher activity. To further improve PLDPMFsynthesis, a secretory expression system suitable for PLDPMFwas constructed and optimized with different signal peptides. The highest secretory efficiency was observed when the PLD * (PLDPMFwith the native signal peptide Nat removed) was expressed fused with the fusion signal peptide PelB-Nat inE. coli. The fermentation conditions were also investigated to increase the production of recombinant PLD and 10.5 U/mL PLD was ultimately obtained under the optimized conditions. For the application of recombinant PLD to PS synthesis, the PLD properties were characterized and 30.2 g/L of PS was produced after 24 h of bioconversion when 50 g/L phosphatidylcholine (PC) was added.Keywords:phospholipase D;phosphatidylserine;secretory expression;enzymatic catalysis
研究领域:
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）