INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Chao Xu,Jun Zhang,Yu Zhang,Ying Guo,Huijuan Xu,Cuiyi Liang,Zhongming Wang,Jingliang Xu
Sugarcane bagasse (SCB) was pretreated by sodium hydroxide (SH), alkaline ethanol (AE) and alkaline hydrogen peroxide (AHP), and the solid residue with similar lignin content after different pretreatment was selected. Enzymatic saccharification with different substrates was performed at 2%, 10%, and 20% ( w / v ) solid loading. After that, the lignin from different substrate was extracted and its structure was characterized. Furthermore, the adsorption capability of isolated lignin to cellulase and its effect on enzymatic hydrolysis were studied. The results showed that, as the substrate content increased from 2% (w/v) to 20% (w/v), the glucose yield after digestion of SH, AE, and AHP pretreated SCB reduced by 41.8%, 35.4%, and 28.7%, respectively. The inhibitory effect of different prepared lignin on the digestibility of Avicel was as follows, SH pretreated lignin (SHL) > cellulase enzymatic hydrolysis lignin (CEL) > AE pretreated lignin (AEL) > AHP pretreated lignin (AHPL), which exhibited positive correlation with its non-productive adsorption capability to cellulase. Lignin samples with low negative zeta potential , high hydrophobicity and high ration of syringyl (S) to guaiacyl (G) were unfavorable for the enzymatic saccharification of cellulose .
研究领域:
生物化学
·
可再生资源工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advances in Materials Science and Engineering
·
2014
·
IF 0.897
M. S. H. Al-Furjan,Kui Cheng,Wenjian Weng
Electrochemical biosensors are essential for health monitors to help in diagnosis and detection of diseases. Enzyme adsorptions on biosensor electrodes and direct electron transfer between them have been recognized as key factors to affect biosensor performance. TiO2 has a good protein adsorption ability and facilitates having more enzyme adsorption and better electron transfer. In this work, Mg ions are introduced into TiO2 nanodots in order to further improve electrode performance because Mg ions are considered to have good affinity with proteins or enzymes. Mg doped TiO2 nanodots on Ti substrates were prepared by spin-coating and calcining. The effects of Mg doping on the nanodots morphology and performance of the electrodes were investigated. The density and size of TiO2 nanodots were obviously changed with Mg doping. The sensitivity of 2% Mg doped TiO2 nanodots based biosensor electrode increased to 1377.64 from 897.8 µA mM−1 cm−2 and its decreases to 0.83 from 1.27 mM, implying that the enzyme achieves higher catalytic efficiency due to better affinity of the enzyme with the Mg doped TiO2. The present work could provide an alternative to improve biosensor performances.
研究领域:
生物医学工程
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材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2018
·
IF 6.14
Kai Wu,Wenjun Ying,Zhengjun Shi,Haiyan Yang,Zhifeng Zheng,Jiayan Zhang,Jing Yang
Nonproductive adsorption of cellulase on lignin was a major inhibitory mechanism reducing the enzymatic saccharification efficiency of lignocellulosic material. The surface and structural properties of Fenton reaction-oxidized lignins (FRLs) and their effects on the nonproductive binding of enzymes to lignin and enzymatic saccharification of Avicel were examined. The Fenton chemistry pretreatment not only decreased the ratio of syringyl to guaiacyl units and the phenolic OH group content in lignin preparations but increased the carboxylic content and the negative zeta potential of lignin, all of which could be responsible for reducing the unproductive adsorption of cellulase on lignin. And Langmuir adsorption isotherms also proved that FRLs were provided with a lower binding ability to the cellulase enzyme when compared with untreated milled bamboo lignin (MBL). Finally, when the nonproductive binding of cellulase to FRLs was reduced significantly, the 72 h glucose yields of Avicel with 3-FRL and 5-FRL were 60.64% and 65.96%, respectively, which were 6.3–15.6% higher than that of Avicel alone, 57.06%. The results can also help to optimize the existing pretreatment technologies and develop new pretreatment methods that could produce less inhibitory lignin.
研究领域:
生物化学
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生物质能
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Energy Technology
·
2018
·Vol. 6
·期 9
·1755-1762
·
IF 3.175
Kai Wu,Wenjun Ying,Zhengjun Shi,Haiyan Yang,ZhiFeng Zheng,JiaYan Zhang,Jing Yang
To screen and evaluate possible functional factors in the bamboo lignin moiety that could block non-productive adsorption of enzyme proteins on lignin, milled wood lignin (MWL) and Fenton oxidized lignin (FOLs) were prepared and incorporated in the enzymatic hydrolysis of Avicel. The addition of MWL decreased the 72 h glucose yields of cellulose from 57.1 % to 45.3 %, while FOLs effectively improved the enzymatic digestibility up to 69.5 %, which was 22 % higher than the system without the use of FOLs. The adsorption affinity and binding strength of cellulase with FOLs were lower than that with MWL. After Fenton pretreatment, hydrophilicity of lignins increased due to formation of C−O− and O−C=O bonds. Furthermore, high surface negative charges on FOLs resulted in strong electrostatic repulsion against the negatively charged cellulases. Finally, analysis of FOLs with 13C and 2D-HSQC NMR indicated that the cleavage of β-O-4 linkages and demethoxylation occurred, and that less condensed structures at the C β and C γ positions were observed. These oxidation reactions of the bamboo lignin lead to the formation of functional factors to alleviate irreversible adsorption of cellulase onto FOLs, and thus were responsible for promoting enzymatic digestibility efficiency.
研究领域:
生物化学
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生物质能
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林业科学
✓ 已验证
DOI
高影响因子
CELLULOSE
·
2020
·Vol. 27
·期 14
·7911-7927
·
IF 4.21
Li Mingfu,Yi Liu,Bin Luo,Zhang Qingtong,Song Junlong,Jiang Hongrui,Chen Changzhou,Wang Shuangfei,Min Douyong
Lignin, as a main factor inhibiting the enzymatic hydrolysis of lignocellulose, was investigated by comparing lignin isolated from untreated and pretreated lignocellulose to elucidate the differences in the enzyme adsorption ability. The nonproductive adsorption of cellulase on lignin was investigated by using a quartz crystal microbalance with dissipation. The results indicated that more cellulase adsorbed on the lignin isolated from autohydrolysis and green liquor-pretreated lignocellulose than on protolignin. Higher temperature and enzyme concentration increased the initial adsorption rate and the amount of enzyme adsorbed on lignin. A dynamic equation for enzyme adsorption on lignin was established. The adsorption of enzymes on lignin increased as the content of phenolic hydroxyl groups, and β-β and β-5 linkages in the lignin increased, attributed to enhanced hydrogen bonding and hydrophobic interaction. Consequently, inhibition of the enzymatic hydrolysis of cellulose was promoted by a higher phenolic hydroxyl group, β-β, and β-5 content. The adsorption of the enzyme components on lignin followed the order: β-glucosidase > xylanase > endo -glucanase > cellobiohydrolase, as revealed by polyacrylamide gel electrophoresis analysis. Electrostatic interaction increased the adsorption of β-glucosidase and xylanase on lignin, while synergistic hydrophobic interaction and hydrogen bonding increased the adsorption of endo -glucanase and cellobiohydrolase on lignin. The results indicate that the adsorption of enzymes on lignin is affected by the structure of lignin and the composition of the enzymes. The findings of the current study provide fundamental knowledge for improving the enzymatic conversion of lignocellulose.
研究领域:
生物化学
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可再生资源工程
✓ 已验证
DOI
高影响因子
JOURNAL OF BIOTECHNOLOGY
·
2024
·
IF 4.1
Chunyang Yu,Zekang Wang,Xiangjin Fu,Chun Liu,Anping Li,Qinlu Lin,Tianqing Lan,Xinshu Zhuang
Lignin can affect the enzymatic hydrolysis efficiency of lignocellulose. In this study, the lignin isolated from sugarcane bagasse (SCB) pretreated with p-toluenesulfonic acid (PL) was firstly aminated, and then the effects of PL and aminated PL (APL) on the bagasse enzymatic hydrolysis efficiency (EHE) were investigated. The results showed that the addition of PL and APL promoted the EHE, and EHE with APL (73.82 %) was higher than PL (51.39 %). To explore the reason, the data were further analyzed including cellulase adsorption capacity, enzyme activity, cellulase-lignin interaction, and molecular docking. It was found that APL adsorbed more cellulase (27.83 mg protein/g lignin) than PL (4.96 mg protein/g lignin), resulting from the greater interaction force and lower binding free energy between APL and cellulase. The addition of APL more remarkably enhanced the cellobiohydrolase and endoglucanase activities than PL due to more effectively inducing cellulase conformation optimization.
研究领域:
生物化学
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可再生资源与环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2023
·
IF 11.4
Lan Yao,Mengzhen Chai,Pingping Cui,Chang Geun Yoo,Jie Yuan,Xianzhi Meng,Haitao Yang
Valorization of lignocellulose has received a lot of attention due to the abundance of lignocellulosics. It was showed that synergistic carbohydrate conversion and delignification could be achieved via ethanol assisted DES (choline chloride/lactic acid) pretreatment. To explore the reaction mechanism of lignin in the DES, milled wood lignin obtained from Broussonetia papyrifera was subjected to pretreatment at critical temperatures. The results suggested that ethanol assistance could contribute the incorporation of ethyl groups and reduce condensation structures of Hibbert’s ketone. Adding ethanol at 150 °C not only decreased formation of condensed G unit (from 7.23% to 0.87%), but also removed J and S’ substructures, thus effectively reducing the adsorption of lignin on cellulase, and promoting the glucose yield after enzymatic hydrolysis.
研究领域:
生物化学
·
可再生资源工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOTECHNOLOGY
·
2025
·
IF 3.9
Lan Yao,Zhe Zhang,Guangyu Chen,Zhiyuan Sun,Xiong Chen,Haitao Yang
The enzymatic hydrolysis of lignocellulose continues to be encumbered by elevated production costs and diminished cellulase efficiency. In this work, modified DES recovered lignin was obtained by grafting acrylamide and acryloyl chloride to enhance glucose release. At a cellulase dosage of 5 FPU/g-cellulose and pH of 5.5, modified lignin promoted glucose yield of dilute-acid-pretreated wheat straw by 158% compared with control. The mechanism by which modified lignin promotes enzymatic hydrolysis was further explored. The binding constant was reduced from (3.3510±0.8361)*10 4 to (2.7600±0.6027)*10 3 L•mol -1 after modification. Modified lignin could make α-helix content enhancement so that cellulase had a compact and stable spatial structure. Lignin binds within the catalytic tunnel of cellulase and that the modified lignin interacts with cellulase with increased hydrogen bonding, resulting in a more compact cellulase structure. The modified lignin might reduce the unproductive adsorption of cellulase, and increase stability and cellulose accessibility to reduce cellulase cost.
研究领域:
生物化学
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生物质能
·
酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Jing Li,Na Fan,Xin Wang,Zhonggui He
The present work initially evaluated cellular toxicity and uptake of our previous biomimetic bimodal nanoporous silica (B-BNS) and applied it as lysozyme adsorbent, which aimed to study potential ability of B-BNS as antitumor biological macromolecules carrier. To highlight the advantage of bimodal mesopores, comparisons were made between single mesoporous silica nanoparticles (S-MSN) and B-BNS. Cell evaluation work was conducted using MCF-7 cells and lysozyme adsorption process was studied with pH and lysozyme concentration as independent variables. The results indicated that the toxicity of S-MSN and B-BNS on MCF-7 cell could be neglected. In addition, S-MSN and B-BNS had the ability to be uptaken into cells and even nucleus evidenced by inverted fluorescence microscope and confocal laser scanning microscopic. Compared to S-MSN, B-BNS adsorbed larger amount of lysozyme due to its bimodal mesopores. Lysozyme adsorption was favorably approximated by the pseudo-second order model. The equilibrium data of lysozyme adsorption were fitted to the Langmuir isotherm model much better than the Freundlich isotherm model, suggesting that lysozyme adsorption on B-BNS via the monolayer adsorption process. Overall, B-BNS can be considered as good antitumor biological macromolecules carrier.
研究领域:
材料科学
·
生物医学工程
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2018
·
IF 3.789
Peiqian Zhang,Mimi Chen,Yuhao Duan,Renliang Huang,Rongxin Su,Wei Qi,Wim Thielemans,Zhimin He
Effective regulation of cellulase adsorption is key to improving the efficiencies of the two major bottlenecks of lignocellulose hydrolysis and cellulase recovery. In this work, we investigated the effect of inhibitors, pH, and temperature on the adsorption of exo- and endoglucanases (Cel7A and Cel7B, respectively) on cellulose using quartz crystal microgravimetry with dissipation. The addition of glucose and cellobiose can both inhibit the hydrolysis activity of Cel7A, whereas only cellobiose can inhibit that of Cel7B. Notably, the adsorption was favored by acidic conditions (pH ≤ 4.8) and low temperature, whereas alkaline conditions (pH 9 and 10) facilitated enzyme desorption, which is useful to guide the process of cellulase recovery. The adsorption and hydrolysis activity of Cel7A and Cel7B were both higher at 45 °C than at 25 °C. These findings pave the way to effective regulation of cellulase adsorption and thus improve lignocellulose conversion and cellulase recovery.
研究领域:
生物化学
·
可再生能源
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FERROELECTRICS
·
2022
·
IF 0.695
Bing Yu,Shuai-Bing Guo,Xue-Xue Han,You-Qing Shen,Hai-Lin Cong
In this paper, we provide the adsorption behavior of lysozyme on the inverse colloidal crystal (ICC) membrane under different parameters (such as temperature, pH value, adsorption time). Besides, this also studied lysozyme adsorption behavior by changing the Hydroxyethyl methacrylate (HEMA) ratio of the ICC membrane. Furthermore, we found that ICC membranes containing Hydroxyethyl methacrylate have significantly improved lysozyme adsorption than ICC membranes without Hydroxyethyl methacrylate. With the increasing lysozyme concentration, the ICC membrane containing 50% HEMA showed an excellent selective adsorption effect. At the same time, we describe the adsorption mechanism of lysozyme on the ICC membrane containing HEMA. Finally, after ten adsorption-desorption cycles on the ICC membrane, the membranes adsorption effect on lysozyme has not significantly decreased.
研究领域:
生物材料学
·
蛋白质工程
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS CHEMISTRY
·
2012
·Vol. 22
·期 18
·9019-9026
·
IF 5.968
Qian Li,Kui Cheng,Wenjian Weng,Piyi Du,Gaorong Han
Uniformly dispersed and size-controllable TiO2 nanodots (TN) were directly synthesized on indium-tin-oxide (ITO) electrodes and were further utilized to immobilize horseradish peroxidase (HRP) enzyme and facilitate direct electron transfer for construction of a hydrogen peroxide (H2O2) biosensor. The enzyme adsorption ability of TN/ITO electrodes were investigated using a UV–vis spectrometer, and the results indicated that TiO2 nanodots possess better enzyme adsorption ability than TiO2 film, and this ability could be further improved by varying the mean diameter of the nanodots from 30 nm to 79 nm. The sensitivity of the H2O2 biosensors based on Nafion/HRP/TN/ITO electrodes was significantly increased by adjusting the dimension of TiO2 nanodots on the modified electrode and the sensitivity differences among these electrodes are in accord with their enzyme adsorption ability. TiO2 nanodots with the largest diameter (79 nm) on a ITO electrode had the best enzyme adsorption ability and biosensor performance with a high sensitivity of 1176 μA mM−1 cm−2 as well as a wide linear range of 1–780 μM. These results show that well-dispersed, high surface area and high curvature TiO2 nanodots could be a promising material for protein adsorption and fabrication of electrochemical biosensors.
研究领域:
生物传感技术
·
材料科学
·
酶工程
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）