Polymers
·
2022
·Vol. 14
·期 21
·4515
·
IF 4.967
Jiankun Xu,Shanmeng Lin,Hao Hu,Qi Xing,Jin Geng
Bufalin (buf) has poor solubility in aqueous solution, poor tumor targeting, and many non-specific toxic and side effects. The advantages of high-molecular-weight polymer conjugates are that they can improve the water solubility of buf, prolong plasma half-life, and reduce non-specific toxicity. A novel water-soluble polymer–drug conjugate with buf and fluorescein pendants was prepared by the combination of reversible addition-fragmentation transfer (RAFT) polymerization and click chemistry. Its anticancer performance and cellular uptake behavior against liver cancer were investigated in vitro. The polymer–buf conjugates exhibit controlled release and tumor-targeting capabilities, showing promise for clinical applications.Keywords:polymer–drug conjugate;liver cancer;esterase responsive;bufalin
研究领域:
药学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
ACS Agricultural Science & Technology
·
2024
·
IF 2.3
Leiyu Yang,Yifan Luo,Yapeng Chen,Sanyan Wang,Huashan Wang,Meiyi Wang
Fomesafen serves as a widely employed selective herbicide for addressing broadleaf weeds, but the short duration of efficacy limits utilization efficiency. There exists an exigent requirement to extend its efficacy through controlled release mechanisms. Fomesafen@SiO2-starch microspheres with α-amylase responsiveness were synthesized through the direct binding of drug-loaded silica microspheres and modified starch. This fabrication method capitalizes on the enzymatic degradation potential of the outer starch layer by amylase. In contrast with earlier analogous structures that exhibited inferior drug loading efficacy, the prepared fomesafen@SiO2-starch microspheres demonstrated a significantly enhanced drug loading capacity of up to 35.7%. Furthermore, in comparison to fomesafen technical, the prepared fomesafen@SiO2-starch microspheres exhibited a notable capacity to mitigate the photolysis of fomesafen through the utilization of the starch outer layer. Additionally, the fomesafen@SiO2-starch microspheres demonstrated favorable wettability and adhesive properties. At the recommended dosage, the herbicidal efficacy of fomesafen@SiO2-starch microspheres against Brassica napus L. and Portulaca oleracea L. over 14 days was observed to be comparable to that of the fomesafen technical, concurrently exhibiting a degree of sustained release. These findings underscore the potential of microspheres in regulating the release of fomesafen, thereby presenting a promising avenue for the development of sustainable drug delivery systems in agriculture.
研究领域:
农业科学
·
材料科学
·
植物保护学
✓ 已验证
产品关联
DOI
高影响因子
GREEN CHEMISTRY
·
2022
·Vol. 24
·期 9
·3667-3676
·
IF 11.034
Bin He,Pengbing Chang,Sufeng Zhang,Xing Zhu
Encapsulating toxic insecticides in a system to reduce their impact on non-target organisms and the environment, thereby enhancing pest control specificity and ensuring human safety, is important but there exist numerous challenges. In the present study, a cellulase-responsive carrier for the controlled release of avermectin (AVM) was prepared based on poly (ethylene glycol) diacrylate (PEGDA)-anchored porous regenerated cellulose microspheres (RCMs), with the goal of realizing their application as insecticide carriers for enzyme-mediated controlled release. Since the as-prepared RCMs showed an excellent loading ability for AVM, biocompatible PEGDA was used to block cellulose micropores and prevent the leakage of AVM. Thus, human safety was ensured. The results show that the cell viability was maintained at 90% in the AVM-loaded microspheres group after 5 days while it was only 70% in the free AVM group, indicating that the system is considerably safer for the human body. Furthermore, the AVM-loaded microspheres group showed excellent toxicological properties against Plutella xylostella and mole cricket. The lethality rates against the two target insects were 90 and 82%, respectively, at a concentration of 60 mg L−1, indicating the high efficacy of the insecticide. This work provides a feasible strategy to eliminate the toxic side effects of insecticides, which is of great significance to agricultural safety.
研究领域:
农业科学
·
生物材料学
·
环境科学
✓ 已验证
DOI
高影响因子
Polymer Chemistry
·
2017
·Vol. 9
·期 4
·438-449
·
IF 5.375
Zhen Zhang,Yongcun Zhou,Zhuxian Zhou,Ying Piao,Nagendra Kalva,Xiangrui Liu,Jianbin Tang,Youqing Shen
Stimuli-responsive dendrimers are attractive nanocarriers for cancer drug delivery due to their inherent structure, biodegradability and precise control of release of a payload in response to specific triggers. However, the efficient and precise synthesis of such dendrimers with site-specific responsiveness is challenging. We report an efficient synthesis of enzyme-responsive phosphoramidate (PAD) dendrimers from a pair of monomers using click and click-like reactions. These dendrimers were stable in phosphate buffered saline (PBS) but degradable in the presence of phospholipase C (PLC) that was overexpressed on cancer cells. The surface modification of the dendrimers with zwitterionic groups (2-methacryloyloxyethyl phosphorylcholine, MPC) greatly reduced the nonspecific binding of the plasma proteins and therefore enhanced blood circulation time. The PAD-MPC dendrimers easily encapsulated hydrophobic drugs such as doxorubicin (DOX). The DOX-loaded PAD-MPC (PAD-MPC/DOX) showed PLC-responsive drug release and efficient delivery of DOX to cancer cells by overcoming multidrug resistance. The PLC-responsive PAD-MPC/DOX was highly toxic to cancer cells and less effective on normal cells. The PAD-MPC/DOX showed improved therapeutic effects and reduced toxicity in athymic nude mice bearing xenografts of MCF-7/ADR breast cancer.
研究领域:
药学
·
生物医学工程
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
Science China-Chemistry
·
2018
·Vol. 61
·期 10
·1314-1319
·
IF 4.448
Liu Rui,Shi Zhekun,Sun Jing,Li Zhibo
Smart peptide hydrogels are of great interest for their great potential applications. Here, we report a facile approach to prepare a class of enzyme-responsive hydrogels in a scalable manner. These hydrogels self-assemble from a family of nonionic peptide amphiphiles (PAs) synthesized by sequential ring-opening polymerization (ROP) of γ-benzyl- L -glutamate N -carboxyanhydride (BLG-NCA) and L -tyrosine N -carboxyanhydride (Tyr-NCA), followed by subsequent aminolysis. These PA samples can readily form a clear hydrogel with a critical gelation concentration as low as 0.5 wt%. The incorporation of tyrosine residues offers hydrophobicity, hydrogen-bonding interaction and enzyme-responsive properties. The hydrogel-to-nanogel transition is observed under physiological conditions in the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H 2 O 2 ). The obtained PA hydrogels are ideal candidates for the new generation of smart scaffolds.
研究领域:
生物材料
·
化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2022
·
IF 5.318
Changzhu Li,Mei Bai,Xiaochen Chen,Wei Hu,Haiying Cui,Lin Lin
In recent years, the development of functional packaging from natural active compounds has been a promising strategy for microbial control in foods . In this study, the basil essential oil (BEO) was applied as the principal antibacterial agent , and a controlled-release nanofibrous system was engineered with responsiveness to bacterial phospholipase . It indicated that encapsulation by cationic liposome significantly improved the dispersity and stability of BEO during the electrospinning process, and the prepared BEO-loaded cationic liposomes (BCLs) could maintain structural integrity in polymer nanofibers. The resulting BCLs-loaded nanofibers showed an effective antibacterial activity against L. monocytogenes . Additionally, it is worth noting that incorporation of soybean lecithin in nanofiber substrate could accelerate the release of loaded antibacterial agents when exposed to L. monocytogenes , thus obtaining an enhanced antibacterial activity. This could be attributed to the increased sensitivity of nanofibers to the degradation by bacterial phospholipases after incorporation with soybean lecithin. Finally, the application test showed that the prepared antibacterial nanofibrous mats could help maintain the quality of chilled pork during 4-days storage, which indicated their promising potential as active packaging. Given the serious threat of L. monocytogenes to food safety, the present antibacterial nanofibers have wide application prospect for improving microbial safety of chilled meat.
研究领域:
食品科学
·
微生物学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Xuefei Bai,Chenyu Liu,Simiao Yu,Yongxin Pan,Farishta Shafiq,Weihong Qiao
Low solubility and chemical instability are the main problems with insoluble bioactives. Lignin, with its exceptional biological properties and amphiphilicity, holds promise as a delivery system material. In this study, glycerol esters were incorporated into alkali lignin (AL) through ether and ester bonds, resulting in the successful synthesis of three hydrophobically modified alkali lignins (AL-OA, AL-OGL, and AL-SAN-OGL). Subsequently, lignin composite nanoparticles (LNPs@BC) encapsulating β-carotene were prepared using antisolvent and sonication techniques. The encapsulation rates were determined to be 37.69 ± 2.21%, 84.01 ± 5.55%, 83.82 ± 5.23%, and 83.11 ± 5.85% for LNP@BC-1, LNP@BC-2, LNP@BC-3, and LNP@BC-4, respectively, with AL, AL-OA, AL-OGL, and AL-SAN-OGL serving as the wall materials under optimized preparation conditions. The antioxidant properties and UV-absorbing capacity of the four lignins were characterized, demonstrating their efficacy in enhancing the oxygen and photostability of β-carotene. Following 6 h of UV irradiation, LNP@BC-4 exhibited a retention rate of 83.03 ± 2.85% for β-carotene, while storage under light-protected conditions at 25 °C for 7 days retained 73.33 ± 7.62% of β-carotene. Furthermore, the encapsulated β-carotene demonstrated enhanced thermal and storage stability. In vitro release experiments revealed superior stability of LNPs@BC in simulated gastric fluid (SGF), with β-carotene retention exceeding 77% in both LNP@BC-3 and LNP@BC-4. LNP@BC-4 exhibited the highest bioaccessibility in simulated intestinal fluid (SIF) at 46.96 ± 0.80%, that LNP@BC-1 only achieved 10.87 ± 0.90%. The enzymatic responsiveness of AL-OGL and AL-SAN-OGL was confirmed. Moreover, LNPs@BC exhibited no cytotoxicity toward L929 cells and demonstrated excellent hemocompatibility. In summary, this study introduces a novel enzyme-responsive modified lignin that has promising applications in the fields of food, biomedicine, and animal feed.
研究领域:
食品科学
·
生物医学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 38
·9246-9259
·
IF 7
Fusheng Zhang,Gongcui Pei,Baihao Huang,Jianchang Xu,Lijuan Zhang
π–π stacking strategies can enhance the stability performance of delivery platforms but are often restricted by incomplete drug release performance, even with the help of crosslinking strategies. Therefore, there has been considerable interest in enhancing the drug release performance by disrupting the π–π stacking region (structural rearrangements). Herein, we synthesized poly(3-(isobutyloxy)-2-oxopropyl benzoate)-b-poly(2-hydroxybutyl methacrylate)-co-poly((ethylene glycol)methylether methacrylate) [PBOOPMA-b-P(HBMA-co-PEGMA), PHB] and revealed the drug release mechanism of PHB-based micelles. The structural rearrangements derived from the crosslinking strategy were revealed to improve the early release performance by 43–55% using micellar dissolutions. Moreover, the esterase-responsive strategy was elucidated to induce reassembly with 77–79% size variation, intensifying the structural rearrangements, which was also synergistic with the crosslinking strategy. Based on the advantages of improving drug release performance, the esterase-responsive strategy was considered a promising candidate for enhancing late release performance. Meanwhile, it is believed that such responsive modulation (crosslinking, esterase-responsive) in the π–π stacking region will become highly promising for subsequent research. Finally, the biosafety of 95.81% at 400 mg L−1 and drug cytotoxicity of IC50 ≈ 2.5 mg L−1 of PHB-EDE@CPT were also validated, confirming the broad application prospects of PHB-based crosslinked micelles.
研究领域:
药剂学
·
生物材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 43
·27963-27969
·
IF 4.036
Ling-Na Han,Kai-Qiang Wang,Zi-Ning Ren,Xue Yang,Xiao Duan,Sasirekha Krishnan,Abinaya Jaisankar,Jeong-Hui Park,Khandmaa Dashnyam,Wujie Zhang,José Luis Pedraz,Seeram Ramakrishna,Hae-Won Kim,Chang-Feng Li,Li-Hua Song,Murugan Ramalingam
In this study, we report a one-pot synthesis and enzyme-responsiveness of polyethylene glycol (PEG) and glutamic acid (Glu)-based amphiphilic doxorubicin (DOX) prodrug nanomicelles for cancer therapeutics. The nanomicelles were accomplished by esterification and amidation reactions. The nuclear magnetic resonance (NMR) and Fourier transform infrared (FTIR) data confirmed the structure of nanomicelles. The DOX-loaded nanomicelles showed a DLS-measured average size of 107 nm and excellent stability in phosphate-buffered saline (PBS) for 7 days. The drug loading and cumulative release rates were measured by ultraviolet-visible (UV-vis) spectrophotometry at 481 nm. The cumulative release rate could reach 100% in an enzyme-rich environment. Further, the therapeutic efficiency of nanomicelles to cancer cells was determined by cell viability and cellular uptake and distribution using HeLa cells. The cell viability study showed that the DOX-loaded nanomicelles could effectively inhibit the HeLa cell proliferation. The cellular uptake study confirmed that the nanomicelles could be effectively ingested by HeLa cells and distributed into cell nuclei. Based on the collective experimental data, this study demonstrated that the synthesized nanomicellar prodrug of DOX is a potential candidate for cancer therapeutics.
研究领域:
药物科学
·
癌症研究
·
纳米技术
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Pengzhong Shi,Jianghui Qin,Xiaoxiao Wu,Liwen Wang,Tinghong Zhang,Dejun Yang,Xingjie Zan,Dietmar Appelhans
Proteins are considered to be one of the most important highly reproducible and monodisperse building blocks with specific functions in life sciences and material science. Protein capsules and their hybrids composed of protein–polymer conjugates have been intensively explored in drug delivery, catalysis, and cell-mimicking functions. Herein, we present a facile, universal, and efficient method to fabricate the diverse protein capsules, independent of the molecular weight (Mw), isoelectric points (IEP), wettability, amino acid sequence, and functional domains of enumerated proteins. The protein capsules were well characterized by various techniques. Furthermore, their ability to store the original protein functionality was demonstrated, which was mainly embodied in their enzyme responsiveness and good biocompatibility in vitro and in vivo. We believe that these protein capsules have multiple potential applications such as in drug delivery, tissue engineering, catalysis, and other application fields.
研究领域:
材料科学
·
生物技术
·
药学
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Soft Matter
·
2015
·Vol. 11
·期 12
·2488-2493
·
IF 4.029
Xiao-Fang Hou,Yong Chen,Yu Liu
Biocompatible and enzyme-responsive supramolecular assemblies have attracted more and more interest in biomaterial fields, and find many feasible applications especially in the controlled drug release at specific sites where the target enzyme is located. In this work, novel supramolecular nanoparticles were successfully constructed from two biocompatible materials, i.e. a cyclic polysaccharide named sulfato-β-cyclodextrin (SCD) and a protein named protamine, through non-covalent association, and fully characterized by means of atomic force microscopy (AFM) and high-resolution transmission electron microscopy (TEM). Significantly, the disassembly of the resulting nanoparticles can respond especially to trypsin over other enzymes. Owing to their trypsin-triggered disassembly behaviors, these nanoparticles can efficiently release the encapsulated model substrate in a controlled manner. That is, the model substrate can be encapsulated inside the nanoparticles with a high stability and released when treated with trypsin.
研究领域:
生物材料
·
药物递送
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2022
·
IF 4.132
Amir E. Kaziem,Liupeng Yang,Yigang Lin,Hanhong Xu,Zhixiang Zhang
In this work, an enzyme-responsive nanovehicle for improving captan (CAP) contact fungicide bioactivity and translocation in plant tissues was synthesized (CAP-MSNs-β-glucan) by attaching β-glucan to the outer surface of mesoporous silica nanoparticles. CAP-MSNs-β-glucan properties were tested by FTIR, ζ-potential, DLS, XRD, TGA, FE-SEM, and HR-TEM. Cargo protection ability of CAP-MSNs-β-glucan from photolysis and hydrolysis was examined in comparison to CAP commercial formulation (CAP-CF). CAP-MSNs-β-glucan distribution in plant tissues, bioactivity against Fusarium graminearum, and biotoxicity toward zebrafish (Danio rerio) were tested and compared with that of CAP-CF. CAP-MSNs-β-glucan results showed good loading efficacy reaching 18.39% and enzymatic-release dependency up to 83.8% of the total cargo after 20 days of β-glucan unsealing. CAP-MSNs-β-glucan showed significant release protection under pH changes. MSNs-β-glucan showed excellent CAP protection from UV. CAP-MSNs-β-glucan showed better distribution in corn tissues and 1.28 more inhibiting potency to Fusarium graminearum than CAP-CF. CAP-MSNs-β-glucan showed 1.88 times lower toxicity than CAP-CF to zebrafish after 96 h of treatment. We recommend using such formulations to overcome shortcomings of contact fungicides and achieve better and sustainable farming.
研究领域:
农业科学
·
纳米生物学
·
植物病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）