ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Jinyi Zhao,Xiaochen Jin,Mengxing Yan,Chen Huang,Qiang Yong,Zhe Ling
Nanocellulose (NC) derived from lignocellulosic biomass has served as a promising material for functional applications. However, conventional chemical and mechanical processes for NC fabrication are environmentally harmful and energy intensive and may inevitably cause component loss. Herein, a green dual-enzymatic treatment assisted by ball milling strategy was proposed for bamboo pulp to produce NC with varied morphologies and supramolecular structures, as well as the coproduction of monosaccharides and xylooligosaccharides (XOS) with reduced enzyme loading and treatment time. Endoglucanase (EG) and xylanase at various loading ratios were used for adjusting the physical–chemical structures of NC. Mechanistic studies showed the accomplishment of nanofibrillation under shorter ball milling time via retention of hemicelluloses, while longer ball milling time intensified fibril deconstruction, thus exposing cellulose hydrophobic surfaces favorable for subsequent enzymatic hydrolysis. The method was subsequently applied to bamboo holocellulose and microcrystalline cellulose (MCC)/xylan models, which obtained the highest yields of 23.2% glucose, 24.4% XOS, and 21.5% NC. Bamboo holocellulose produced NC with a high aspect ratio of up to 55.1, while MCC produced spherical NC with cellulose II allomorph. The proposed ball-milling-assisted dual-enzymatic treatment can provide theoretical guidance for high-value conversion and more complete utilization of bamboo polysaccharides.
研究领域:
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
TEXTILE RESEARCH JOURNAL
·
2018
·
IF 1.54
Xiang Yeping,Yu Jianyong,Liu Liu,Zhang Ruiyun,Qu Yongshuai,Jing Miaolei
In this study, we found an environmentally friendly system in the degumming of hemp fiber without using a strong acid and alkali. The chemo-enzymatic modification and degumming of hemp fiber used an acetic acid–sodium acetate buffer solution containing laccase, hemicellulase and 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO) under a mild condition (pH = 5, T = 50℃). Through comparing and analyzing the physical and chemical properties of fiber products under different treatments, we found the method involving treatment with TEMPO/laccase/hemicellulase not only greatly removed most of the gum attached on the surface of the raw hemp fiber, but also introduced surface-active aldehyde and carboxyl groups into the fiber. The performances of the hemp fibers with different treatments were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray diffraction. We also found the product was finer, with a lower content of non-cellulosic components and it had better water uptake properties (moisture sorption and water retention value), which has potential application values in textile industries.
研究领域:
材料科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Jie Hao,Xusong Wang,Liqun Li,Zhikang Sun,Xuanwen Li,Qiang Ji,Xuhui Huang,Lei Qin
Tobacco leaves were treated with mesophilicα-amylase, pectinase, and lipase, respectively, of which the perceived aroma and distinctive flavor were evaluated by smoking. Additionally, metabolomics, lipidomics, and flavoromics were combined to further explore the effects of different enzyme treatments on the composition of the tobacco leaves. It was found that the irritancy and offensive taste of the tobacco leaves were reduced, and the quality of aroma was improved by enzyme treatment. Pectinase-treated tobaccos were rated highest for sweet aroma, while lipase-treated tobaccos excelled at improving the concentration and volume of aroma. The total relative abundances of lipid and lipid-like molecules in tobacco leaves were markedly diminished following treatment with either pectinase or lipase. Besides, the acid volatiles content increased by 18.96 % after mesophilicα-amylase treatment, and decreased by more than 35 % after separate treatment with pectinase or lipase. The ester content was reduced by over 22 % after treatment with pectinase and lipase, respectively. These results provide a feasible approach for improving the sensory quality of tobacco through targeted enzyme treatment.
研究领域:
植物学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
TEXTILE RESEARCH JOURNAL
·
2022
·
IF 2.455
Weini Jia,Yiqing Yao,Jianmin Guo,Xiaoli Zhang,Zhijie Liang
Surface modification of polypropylene is performed in this work by enzyme-catalyzed post-processing strategy. The polyDOPA was synthesized by in-situ polymerization in the present of laccase and deposited on the polypropylene surface. The effect of various process variables namely processing step, pH, time, and temperature is studied at various levels. The results show that one-bath and one-step method is the optimal mode. Various functional groups incorporated to the polymer surface are confirmed by attenuated total reflectance-Fourier-transform infrared spectroscopy. The effect of process variables on surface morphology of polymers is evaluated by scanning electron microscopy, revealing that the surface of the polypropylene is covered with thin burrs, and these are regarded as polyDOPA adhered after treatment. Furthermore, the electrostatic charge decay time and contact angle as well as capillary wetting measurements of the modified polypropylene are 0.61 s and 75.33°, respectively, indicating its fine anti-static electricity and better wettability. Besides, this material shows dark gray colors and exhibits excellent ultraviolet-resistance performance. Interestingly, the modification process nearly has no influences on the mechanical property.
研究领域:
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Polymers
·
2023
·Vol. 15
·期 19
·3879
·
IF 5
Xiangyu Chen,Jiahua Ma,Xiaoshuai Sun,Chuanshan Zhao,Jiehua Li,Hui Li
This paper proposes a different strategy for deriving carbon materials from biomass, abandoning traditional strong corrosive activators and using a top−down approach with a mild green enzyme targeted to degrade the pectin matrix in the inner layer of pomelo peel cotton wool, inducing a large number of nanopores on its surface. Meanwhile, the additional hydrophilic groups produced via an enzymatic treatment can be used to effectively anchor the metallic iron atoms and prepare porous carbon with uniformly dispersed Fe−Nxstructures, in this case optimizing sample PPE−FeNPC−900’s specific surface area by up to 1435 m2g−1. PPE−FeNPC−900 is used as the electrode material in a 6 M KOH electrolyte; it manifests a decent specific capacitance of 400 F g−1. The assembled symmetrical supercapacitor exhibits a high energy density of 12.8 Wh kg−1at a 300 W kg−1power density and excellent cycle stability. As a catalyst, it also exhibits a half−wave potential of 0.850 V (vs. RHE) and a diffusion-limited current of 5.79 mA cm−2at 0.3 V (vs. RHE). It has a higher electron transfer number and a lower hydrogen peroxide yield compared to commercial Pt/C catalysts. The green, simple, and efficient strategy designed in this study converts abundant, low−cost waste biomass into high-value multifunctional carbon materials, which are critical for achieving multifunctional applications.Keywords:pomelo peel;enzymatic treatment;Fe−Nx;supercapacitors;ORR
研究领域:
材料科学
·
能源科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Jie Hao,Xusong Wang,Liqun Li,Xuhui Huang,Na Wu,Tao Yu,Lu An,Lei Qin
The effect of exogenous enzyme treatment on hazardous substances and small-molecule compounds in smoke was investigated by treating Yunyan 87 tobacco with mesophilicα-amylase, pectinase, and lipase. The three enzymes could effectively diminish the total particulate matter (TPM) and nicotine-free dry particulate matter (NFDPM) content in smoke. Notably, mesophilicα-amylase demonstrated the most significant reduction, achieving a decrease of 8.15 % in TPM and 7.08 % in NFDPM content. The efficacy of these three enzymes in reducing phenol levels was remarkable, with a reduction exceeding 20 %. Pectinase and lipase exhibited superior performance in diminishing hydrogen cyanide (HCN) content, achieving a reduction of 13 %. The relative abundance of aroma-related and alkaloids compounds in smoke exhibited varying alterations after different enzyme treatments. The utilization of mesophilicα-amylase exhibited a favorable influence on the augmentation of aroma-related compounds, such as β-damascone and α-cyperone, whereas the effect of pectinase was opposite. The application of pectinase played a crucial role in the reduction of tobacco alkaloids and their related compounds. The occurrence of defects is unavoidable during the growth and curing process of tobacco leaves. Utilizing the characteristics of high efficiency and specificity of enzyme preparation for targeted modification of tobacco leaves is a viable method that can be tried to improve the quality of tobacco leaves.
研究领域:
生物学
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医学
·
烟草科学
✓ 已验证
产品关联
DOI
高影响因子
NORDIC PULP & PAPER RESEARCH JOURNAL
·
2023
·Vol. 38
·期 3
·449-457
·
IF 1.2
Chen Gong,Shujie Fan,Bin Yang,Yu Zhang,Jianping Ni,Zhenhua Su
The aim of this study was to investigate an effective approach for improving the dissolving pulp properties for making specialty fiber. The caustic and enzymatic treatment of the properties of dissolving pulp was conducted by analyzing the macromolecular structure and chemical composition. The results showed that the enzymatic treatment was more effective on influencing the macromolecular properties, while the caustic treatment had more influence on structural changing, mainly cellulose crystal structure and crystallinity. Meanwhile, to maintain a higher purity and higher brightness, caustic treatment would be beneficial in comparison with enzymatic treatment. The performance of caustic treated sample was evaluated in regarding to chemical composition and acetylation reaction performance, using commercial pulp as reference. The competitive feature of treated sample suggested its potential in future application.
研究领域:
制浆造纸工程
·
生物化学
✓ 已验证
DOI
高影响因子
Biomass Conversion and Biorefinery
·
2021
·Vol. 13
·期 2
·583-592
·
IF 4.987
Chen Guozheng,Dong Junjing,Wan Jinquan,Ma Yongwen,Wang Yan
In response to deal with the increasingly serious environmental problems and shortage of fiber raw materials, biological enzyme pretreatment is an effective way to replace a large number of chemical additives to improve the properties of waste paper fibers. Fiber characterization of old corrugated container bleached pulp with laccase and glycine (Lac/Gly) pretreatment was investigated by means of Fourier transform infrared spectroscopy (FTIR), headspace gas chromatography (HSGC), fiber quality measurements (FQA), X-ray diffraction method (XRD), and atomic force microscope (AFM). Results showed that, compared with the control pulp, the whiteness and brightness of the Lac/Gly-treated pulp increased by 16.17% and 7.41%, respectively. And the FTIR showed that Lac/Gly pretreatment promotes the effective removal of lignin by hydrogen peroxide (H 2 O 2 ) bleaching. The content of carboxyl groups in pulp increased remarkably by 21.92%. The paper physical analysis showed that the paper strength properties have improved remarkably. The fiber quality analyses indicated that the fiber length, coarseness, and curl index changed a little. The XRD analysis showed that the crystallinity decreased by 5.83% due to Lac/Gly treatment. The AFM analysis showed that through Lac/Gly treatment, the lignin and extracts over the fiber surface are decreased significantly.
研究领域:
制浆造纸工程
·
环境科学
·
生物技术
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2020
·Vol. 55
·期 9
·3182-3189
·
IF 2.773
Zhang Ye,Xing-He Tan,Zhi-Wei Liu,Rana Muhammad Aadil,Yi-Cheng Tan,Muhammad Inam-ur-Raheem
Summary The robust cell wall structure of Haematococcus pluvialis ( H. pluvialis ) consists of polysaccharides and tough non-hydrolysable sporopollenins, which makes it difficult to extract superpotent antioxidant, astaxanthin from these cells. Therefore, breakdown of cell wall is a key step in the overall process of astaxanthin recovery. In this study, the mechanism of three well-established chemical techniques for cell disruption of H. pluvialis cysts [ionic liquids (IL), hydrochloric acid (HCl) and multiple enzymes (multi-enzyme, ME)] on deconstruction of the cyst cell wall of H. pluvialis was explored and characterised by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), nuclear magnetic resonance (NMR) and gas chromatography–mass spectrometry (GC-MS) analyses. The results demonstrated that the three cell wall breakdown techniques exhibited high extraction efficacies for the recovery of astaxanthin from H. pluvialis [IL (86.71 ± 2.06%), HCl (80.52 ± 2.28%) and ME (71.08 ± 2.49%)]. However, their performances on disrupting the trilayered cell walls of H. pluvialis were significantly different, which were confirmed by distinct morphologies of the treated cell walls visualised by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Meanwhile, the results of FTIR confirmed that, to some extent, cellulose, hemicellulose and lignin in the cell walls were hydrolysed by HCl, IL and ME treatments. However, ME exhibited a less hydrolytic effect on lignin than HCl and IL. Moreover, XRD and NMR analyses implied that the amorphous region of cell wall was susceptible to hydrolysis/breakdown by the three techniques.
研究领域:
生物化学
·
植物学
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD QUALITY
·
2022
·
IF 3.2
Xuan Ji,Jing Du,Jiaying Gu,Jie Yang,Li Cheng,Zhaofeng Li,Caiming Li,Yan Hong
In order to improve the ability of starch to absorb menthone, corn starch was modified by enzymatic treatment (amyloglucosidase and α-amylase) combined with octenyl succinic anhydride (OSA) esterification. The oil absorption rate of starch modified by enzymatic treatment followed by OSA (P-OSA) reached 101.33%, whereas that of samples with reverse action sequences (OSA-P) was only 59.67%. The degree of substitution of OSA-P was also generally lower than that of P-OSA. At high OSA addition, OSA-P had a smaller specific surface area with fewer pores because octenyl succinic (OS) groups impeded the enzymatic treatment. Compared with OSA-P, the lamellar structure of P-OSA is sparser and less ordered. Owing to its pores, P-OSA was beneficial for the reaction to occur inside the granules, which was observed by Raman spectroscopy and laser confocal microscopy. At high OSA addition, the loading of P-OSA to menthone could reach 64.34 mg/g.
研究领域:
食品科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jiaqi Zhang,Kun Li,Jun Wang,Bo Xu,Ying Liu,Qiang Wang,Ping Wang
Proteases have emerged as promising agents for wool anti-felting finishing due to their eco-friendly characteristics. However, while natural proteases are used to hydrolyze the scale layer, their tendency to penetrate into the interior of the fiber often leads to undesirable consequences, including unacceptable damages, and reduced color depth of the dyed textiles. To address these limitations, a combined approach involving mild reduction treatment and controlled protease incubation was developed. This strategy specifically targets the reduction of adverse effects associated with protease treatment, particularly irreversible fiber damage and dye leaching. Specifically, a sodium bisulfite pre-reduction was employed to loosen the highly cross-linked scale layer in dyed wool, followed by protease treatment targeted to enzymatic hydrolysis of wool scales. Using a two-bath process with 5 g/L sodium bisulfite and 8 U/mL protease of Savinase Ultra 16XL, the treated wool fabric demonstrated low felting shrinkage (4.14 %), acceptable strength loss (6.58 %), and minimal alteration in color depth (color difference value <1.1). The present strategy overcomes the drawbacks of conventional protease treatments, particularly the strength loss and color alteration typically observed in dyed fabrics, providing an eco-friendly way for the commercial application of anti-felting finishing of the dyed wool textiles.
研究领域:
纺织科学
·
生物技术
·
生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2020
·Vol. 32
·期 4
·045605
·
IF 3.551
Runhong Wei,Ruhui Xu,Keyu Zhang,Feng Liang,Yaochun Yao
Silicon/carbon composites have the disadvantages of large volume expansion and high cost, which limits their commercial application. In this study, green and economic starch was used to prepare porous starch (PS) under the action of enzymes, and then nano-silica was embedded in the PS. A PS based carbon/silicon/carbon composite was prepared by coating and carbonizing the starch slurry, which can alleviate the volume expansion of silicon. The results show that the anode composite material with 20% silicon content has a high initial capacity of 869 mAh g−1 and an initial Coulombic efficiency of 66% at 0.2 A g−1, and the specific capacity is maintained 450 mAh g−1 after 100 cycles. When the silicon content reaches 30%, the reversible capacity of the composite is 1490 mAh g−1 at a current density of 0.2 A g−1, and the capacity remains 850 mAh g−1 after 100 cycles. Its excellent properties and stability are attributed to the abundant porosity of the carbon in the starch derived layer, which improves the structural stability and electrochemical kinetics. This method provides a reference for the sustainable and environmental protection of lithium-ion battery anode materials.
研究领域:
材料科学
·
生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）