BIOPROCESS AND BIOSYSTEMS ENGINEERING
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2023
·Vol. 46
·期 9
·1331-1340
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IF 3.8
Yu Lishuang,Zou Cheng,Li Qingyun,Liu Zhaoming,Liu Youyan,Tang Aixing
Chemoenzymatic epoxidation of olefin mediated by lipase is a green and environmentally friendly alternative process. However, the mass transfer barrier and lipase deactivation caused by the traditional organic–water biphasic reaction system have always been the focus of researchers’ attention. To overcome these issues, we investigated the effects of reaction temperature and two important substrates (H 2 O 2 and acyl donor) on the epoxidation reaction and interfacial mass transfer. As a result, we determined the optimal reaction conditions: a temperature of 30 °C, 30 wt-% H 2 O 2 as the oxygen source, and 1 M lauric acid as the oxygen carrier. Additionally, by simulating the conditions of shaking flask reactions, we designed a batch reactor and added a metal mesh to effectively block the direct contact between high-concentration hydrogen peroxide and the enzyme. Under these optimal conditions, the epoxidation reaction was carried out for 5 h, and the product yield reached a maximum of 93.2%. Furthermore, after seven repetitive experiments, the lipase still maintained a relative activity of 51.2%.
研究领域:
生物化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Caiyue Zhang,Chenchen Hu,Yanan Sun,Xueying Zhang,Yequn Wang,Hongfei Fu,Xiangwei Chen,Yunyang Wang
Fresh green peas require blanching to terminate enzymatic reaction induced quality deterioration before frozen storage. Radio frequency (RF) heating is a novel way of dry blanching for fruits and vegetables with high processing efficiency. In this study, blanching effects of RF heating on relative activities of lipoxygenase (LOX) and peroxidase (POD), physiochemical properties as well as cellular morphology changes of green peas were investigated. Results showed relative activities of pea LOX and POD reduced to 0.90 ± 0.78% and 1.10 ± 0.71%, respectively at 85 °C by RF heating with an electrode gap 105 mm. Weight loss, color, texture and electrolyte leakage of peas changed significantly with increasing temperature (60–85 °C). Ascorbic acid, chlorophyll and mineral contents had different loss after RF processing and long term heating at 115 mm exacerbated the loss of nutrients. Microstructure features showed the deconstruction of pea cell well and starch granule gelatinization.
研究领域:
食品科学
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植物生理学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2021
·Vol. 57
·期 2
·827-841
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IF 3.713
Muhammad Umair,Saqib Jabbar,Yue Lin,Mustapha Muhammad Nasiru,Jianhao Zhang,Muhammad Abid,Mian Anjum Murtaza,Liqing Zhao
Summary The combined effect of emerging electro-technology high-voltage cold plasma (HVCP) and non-thermal ultra-high hydrostatic pressure (UHP) as bioprocess technologies on the quality and stability of carrot juice has been evaluated. Carrot juice was treated with HVCP (70 kV for 3 min), UHP (300, 400, and 500 MPa) at 25 °C for 5 min, and a blanched sample of 100 °C for 5 min (BS) was also prepared. As compared to control and single treatments, the combined treatment (HVCP-UHP500) has attained better results in terms of enzyme inactivation and electroporation extraction of coloring compounds, phytochemicals, and ascorbic acid. Wherein, the maximum of 25.87 ± 0.12 a µg/100 mL of β-Carotene, 11.89 ± 0.80 a GAE (µg g −1 ) of total phenols, and 24.07 ± 0.12 b mg/100 mL of ascorbic acid content in carrot juice were also observed in the combined treatment at 500 MPa. In addition, phytochemical enhancement might be attributed to the disintegration of phenolic dimers into simple aglycones, predominantly because of the electro-kinetic phenomenon of HVCP, which is subsequently intensified by UHP treatment. Therefore, the current study suggests that the combination of two novel techniques is a highly efficient method in terms of energy, time, and cost, with low yield efficiency and bio-safety.
研究领域:
食品科学
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生物技术
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DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Ying Liu,Wenjuan Qu,Yuxuan Liu,Jamila A Tuly,Haile Ma
Food quality is greatly impacted by traditional heat methods for polygalacturonase (PG) inactivation; therefore, it's imperative to develop a novel infrared (IR) inactivation approach and identify its mechanism. Utilizing molecular dynamics (MD) simulation, this study verified the PG's activity, structure, active sites, and substrate channel under the single thermal and non-thermal effects of IR. PG activity was significantly reduced by IR, and structure was unfolded by increasing random coils (65.62 %) and decreasing β-sheets (29.11 %). MD data indicated that the relative locations of PG's active sites were altered by both IR effects, and the enzyme-substrate channel was shortened (10.53 % at 18 μm and 15.79 % at 80 °C). The thermal effect of IR on the inactivation of PG was significantly more pronounced than its non-thermal effect. This study unveiled the mechanism by which the infrared disrupted PG's activity, active sites, and substrate channels; thus, it expanded the infrared technique's efficacy in enzyme control.
研究领域:
食品科学
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生物物理学
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化学工程
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Shi-Ye Luo,Jia-Li Tao,Yan-Xiang Bi,Hong-Wei Xiao,Hua-Lei Chen,Xiang-Xin Li,Yin-Chen Wang,Xiao-Ming Fang
Crystallization degrades the physicochemical properties of honey and reduces consumer acceptance. To address this issue, radiofrequency was developed to investigate the decrystallization efficiency and quality impact mechanism of rape honey. The results showed that radiofrequency significantly decreased the number and size of crystals, leading to shortening the decrystallization time to less than 10 min. The response surface optimization methodology further indicated that the highest decrystallization rate (98.72 ± 0.34 %) and lower 5-Hydroxymethylfurfural (2.45 ± 0.12 mg/kg) contents were obtained. Furthermore, radiofrequency changed the honey from a pseudoplastic into a Newtonian fluid efficiently due to the volumetric heating feature. It is worth noting that the inactivation of glucose oxidase reduced the antibacterial capacity, while the increase in total phenolic and flavonoid contents improved the antioxidant capacity of rape honey. In summary, current findings indicated that radiofrequency is a potential alternative decrystallization technology for water baths.
研究领域:
食品科学
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化学
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农业
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产品关联
DOI
PubMed
高影响因子
CATALYSIS COMMUNICATIONS
·
2017
·
IF 3.33
Youzhi Zhou,Lu Zhao,Ke Liu,Jinsong Zhang,Jianlin Chu,Bingfang He
Aromatic alcohols are liable to result in enzyme inactivation. A novel strategy was established for improving the aromatic alcohol tolerance of β-galactosidase (BMG) from Bacillus megaterium YZ08. The half-life of BMG in 200 mM vanillyl alcohol solution was dramatically increased by 9–123 times with the addition of hydrophilic solvents. In 30% DMSO, the reaction concentration of aromatic alcohol could reach up to 300–400 mM, and 0.85–2.44 g of natural glycosides were successfully obtained in 100 mL-scale. The simple and effective strategy shows potential applications when dealing with the preparative-scale exploitation of enzymatic reactions.
研究领域:
生物化学
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生物技术
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酶学
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Shouyu Wu,Jiemei Ou,Shuyi Wang,Aiping Zheng,Fan Yang,Zidan Zhou,Xuanxuan Lu
The use of Pickering emulsions for biocatalysis is gaining increased attention. However, the extensive application is greatly limited due to the enzyme inactivation . Herein, a biocatalytic Pickering emulsion with high-performance utilizing cellulose nanocrystals immobilized lipases (CNCs-Lps) particles as stabilizer is advanced and applied for the synthesis of Vitamin E nicotinate . CNCs-Lps display high activity and reusability due to the construction of biocatalytic microreactor in the O/W emulsion system . The yield of vitamin E nicotinate ester reached up to 83 %. More importantly, the CNCs-Lps can be reused due to the similar principles to microreactors in Pickering emulsions . Reusability test showed that the CNCs-Lps could be recovered from the emulsion system by centrifugation and the yield of vitamin E nicotinate retains 78 % of initial value after five cycles, demonstrating overwhelming advantage than the fair counterpart with free lipases .
研究领域:
生物技术
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药学
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材料科学
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产品关联
DOI
PubMed
高影响因子
CHEMICO-BIOLOGICAL INTERACTIONS
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2022
·
IF 5.168
Xu Wang,Lingwen Dong,Guode Zhao,Weiwei Li,Ying Peng,Jiang Zheng
2,3,5,4′-Tetrahydroxy stilbene-2-Ο- β -D-glucoside (TSG) and emodin (EMD) are two main components of Polygonum multiflorum Thunb. (PMT). Its root is widely used as herbal medicine and supplement. However, PMT-induced liver injury has drawn increasing attention. The purpose of this study was to investigate the interaction of TSG with EMD in the aspects of enzymology , pharmacokinetics , and hepatotoxicity. Co-administration with TSG increased internal exposure of EMD, EMD-derived hepatic protein adduction, and EMD-induced liver injury in mice. Mouse and human liver microsomal incubation study demonstrated that co-incubation with TSG decreased the formation of hydroxylation metabolites of EMD. Human recombinant cytochrome P450 enzyme incubation study showed that TSG induced time-, concentration-, NADPH-dependent and irreversible inhibition of CYP2C19 and CYP3A4 . An epoxide metabolite derived from TSG was responsible for the observed enzyme inactivations. The findings allow us to better understand the mechanisms by which herbal processing detoxifies raw PMT.
研究领域:
药理学
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毒理学
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酶学
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中药学
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产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2023
·Vol. 14
·期 1
·1-20
·
IF 16.6
Xu Tao,Ji Haifeng,Xu Lin,Cheng Shengjun,Liu Xianda,Li Yupei,Zhong Rui,Zhao Weifeng,Kizhakkedathu Jayachandran N.,Zhao Changsheng
Clinical use of intraoperative auto-transfusion requires the removal of platelets and plasma proteins due to pump-based suction and water-soluble anticoagulant administration, which causes dilutional coagulopathy. Herein, we develop a carboxylated and sulfonated heparin-mimetic polymer-modified sponge with spontaneous blood adsorption and instantaneous anticoagulation. We find that intrinsic coagulation factors, especially XI, are inactivated by adsorption to the sponge surface, while inactivation of thrombin in the sponge-treated plasma effectively inhibits the common coagulation pathway. We show whole blood auto-transfusion in trauma-induced hemorrhage, benefiting from the multiple inhibitory effects of the sponge on coagulation enzymes and calcium depletion. We demonstrate that the transfusion of collected blood favors faster recovery of hemostasis compared to traditional heparinized blood in a rabbit model. Our work not only develops a safe and convenient approach for whole blood auto-transfusion, but also provides the mechanism of action of self-anticoagulant heparin-mimetic polymer-modified surfaces.
研究领域:
血液学
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外科技术
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材料科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yifan Zhu,Mubango Elliot,Yanhong Zheng,Jing Chen,Dongzhi Chen,Shanggui Deng
Atmospheric cold plasma (ACP) is a novel nonthermal technology with potential applications in maintaining and improving food quality. The effect of ACP on the activity and structure of mushroom ( Agaricus bisporus ) polyphenol oxidase (PPO) was evaluated. Results demonstrated that the dielectric barrier discharge (DBD) based plasma technology could inactivate PPO (up to 69%) at 50 kV with the increased concentrations of H 2 O 2 and NO x . An obvious enhancement of surface hydrophobicity was observed, whereas a gradual reduction of total sulfhydryl content was recorded with the increasing exposure time. Data from circular dichroism, atomic force microscopy, particle size distribution and fluorescence spectra displayed the rearrangement of secondary structure and disruption of the tertiary structure. Red shifts of fluorescence spectra showed positive correlations with the inactivation rate of PPO. Therefore, ACP treatment could be served as an alternative approach to inactivate undesirable enzymes to minimize the loss of food nutrition and quality.
研究领域:
食品科学
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生物技术
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酶学
·
等离子体物理
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Samuel Ariyo Okaiyeto,Shi-Han Yu,Haiou Wang,Jin-Hong Zhao,Cun-Shan Zhou,Ahmed Jamilu Mustapha,An-An Zhang,Fan Yang,Jing-Shou Zhang,Hong-Wei Xiao
This study evaluates vacuum-steam pulsed blanching (VSPB) as a pretreatment for Vigna unguiculata pods, focusing on drying kinetics, nutritional quality, and anti-nutritional factors. Pods underwent 0–4 VSPB cycles, followed by hot air drying at 50, 60, and 70 °C. VSPB reduced peroxidase activity to 4.8 %, enhancing enzyme inactivation. Texture analysis revealed an 85.6 % reduction in hardness, while the Midilli model provided the best description of drying kinetics (R 2 ≥ 0.9979). Specific energy consumption decreased by up to 40 % at 70 °C for blanched samples. VSPB preserved ascorbic acid, vitamin A, and chlorophyll, reduced lignin by 29.63 %, and lowered lectins from 71.01 to 8.88 Hu/mg. Folate retention declined with increased cycles. SEM and TEM analyses revealed microstructural and ultrastructural disruptions enhancing moisture diffusivity. VSPB proves effective in reducing anti-nutritional factors and retaining nutrients, offering a promising pretreatment for Vigna unguiculata with potential industrial applications.
研究领域:
食品科学与技术
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采后加工
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植物食品生物化学
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干燥技术
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营养品质
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Ruixiang Liu,Zhongyu Cai,Qingsong Zhang,Heng Yuan,Guanglei Zhang,De'an Yang
Hydrogen peroxide (H 2 O 2 ) detection is highly desirable for both environment and health concerns and a number of methods have been developed to monitor H 2 O 2 . However, most methods are limited due to the need of sophisticated instrument and high cost. Here we report a label-free two-dimensional (2-D) photonic crystal (PhC) horseradish peroxidase/bovine serum albumin (HRP/BSA) protein composite hydrogel sensor to monitor H 2 O 2 . Our 2-D PhC HRP/BSA protein composite hydrogel sensor is fabricated by mild protein cross-linking of BSA and HRP with glutaraldehyde , where BSA acts as a scaffold of HRP while HRP acts as the recognition molecule. The 2-D PhC HRP/BSA protein composite hydrogel sensor selectively detects H 2 O 2 since HRP can specifically decompose H 2 O 2 accompanying with heme inactivation. This protein hydrogel undergoes a volume phase transition as a result of peroxidase inactivation. The inactivation-induced conformational change of HRP gives rise to a decreased cross-linking density, which enlarges the particle spacing and decreases the Debye ring of the 2-D photonic crystals (PhCs). Our optimized 2-D PhC HRP/BSA-75 protein composite hydrogel sensors show a good linear range from 8.8 × 10 -6 M to 60.6 × 10 -6 M and an excellent sensitivity with a limit of detection of 8.8 × 10 -6 M. This sensor fabrication method demonstrates the proof-of-concept for utilizing proteins with few lysine groups to develop smart PhC protein composite hydrogel sensors to determine H 2 O 2 concentration in solution, which makes it possible for developing protein organogel sensors to detect gaseous H 2 O 2 in the future.
研究领域:
生物传感
·
材料科学
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分析化学
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环境监测
·
医疗保健
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DOI
高影响因子
危险品化学品经营许可证（带存储）