TALANTA
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2025
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IF 6.1
Ling Yang,Jihao Su,Zhixuan Yu,Yanqing Chang,Dengbin Yu,Zhiquan Zhang,Shaojun Dong
In this study, a novel and efficient colorimetric biosensor was constructed for biochemical oxygen demand (BOD) detection based on visual analysis and Qualcomm measurement with enzyme-labeling instrument. Microorganisms cultured in situ with actual water and Prussian blue were used as model bacteria and indicator of colorimetric biosensor, respectively. To accurately detect BOD content in water, the reaction conditions such as cell concentration, K 3 [Fe(CN) 6 ] concentration, reaction temperature, and reaction time were optimized. The optimal conditions were OD 600 = 7, 10 mM, 37 °C and 40 min, respectively. The dominant bacteria in the color reaction process were Acinetobacter , Bacillus and Exiguobacterium . The detection range of the biosensor was 5–80 mg L −1 , and the detection limit was 3.32 mg L −1 . The relative error of BOD content in actual water was 15.49 %. In summary, the high-efficiency colorimetric biosensor can detect BOD content quickly and sensitively, which implied its potential application prospects in the field of BOD detection.
研究领域:
生物化学
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环境监测
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微生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2019
·
IF 3.973
Hongjian Wen,Xinhua Zhou,Zhichuan Shen,Zhicheng Peng,Huayao Chen,Li Hao,Hongjun Zhou
Polyethyleneimine (PEI) was immobilized on SBA-15 for obtaining polyethyleneimine-modified silica [email protected] ( [email protected] , [email protected] , and [email protected] ). With the coordination from PEI, zinc ions were impregnated on the [email protected] , and then, Zn 2+ [email protected] were calcined at 550 ℃ to obtain the nano zinc oxide loaded on the mesoporous silica, ZnO-SBA-15s (ZnO-SBA-1.0, ZnO-SBA-1.5, and ZnO-SBA-2.0). The anti-bacterial activity of ZnO-loaded SBA-15 was; thereafter, assessed by the zone of inhibition and enzyme labeling methods. The results confirmed that the coordination ability of the imino groups in PEI helped adsorb more zinc ions during the process of impregnation so that more nano-ZnO could be supported on the surface of SBA-15. The modification did not change the mesostructure of the two-dimensional hexagonal phase and orderliness of SBA-15. At the mass ratio of PEI:SBA-15 = 1.0:1, 1.5:1, and 2.0:1, the concentration of zinc element (wt.%) was measured by inductively coupled plasma optical emission spectrometry as 2.5, 2.56, and 3.5% for ZnO-SBA-1.0, ZnO-SBA-1.5, and ZnO-SBA-2.0, respectively. The size of inhibition of SBA-15, ZnO-SBA-0, ZnO-SBA-1.0, ZnO-SBA-1.5, and ZnO-SBA-2.0 were 0, 1.14 ± 0.01, 2.56 ± 0.01, 2.58 ± 0.01, and 2.69 ± 0.02 cm, respectively. ZnO-SBA-15s suppressed the growth of Escherichia coli compared to blank SBA-15 in a ZnO dose-dependent manner.
研究领域:
材料科学
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纳米技术
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生物技术
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微生物学
✓ 已验证
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2013
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IF 5.437
Mouhong Lin,Yingju Liu,Xiaofen Chen,Shidong Fei,Chunlin Ni,Yueping Fang,Chengbin Liu,Qingyun Cai
An electrochemical, signal amplified immunosensor was developed to detect 3-bromobiphenyl (BBP) by using a bio-inspired polydopamine (PDOP)/gold nanocluster (AuNc) as the sensor platform and multienzyme-labeled carbon hollow nanochains as the signal amplifier. The self-polymerized dopamine membrane on the AuNc-modified indium tin oxide (ITO) electrode were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), contact angle and electrochemical measurements. Such PDOP/AuNc platform featured the mild cross-linking reaction with the dense immobilization of BBP-antigens (BBP-Ag). Moreover, by using multiple horseradish peroxidase (HRP) and secondary antibodies (Ab 2 ) modified one-dimensional carbon hollow nanochains (CHNc) as the signal enhancer, it held promise for improving the sensitivity and detection limit of the immunoassay . Based on the competitive immunoassay protocol, this immunosensor showed a linear range from 1 pM to 2 nM for BBP with a detection limit of 0.5 pM. Also, it exhibited high sensitivity, wide linear range, acceptable stability and reproducibility on a promising immobilization platform using a novel signal amplifier, which may extend its application in other environmental monitoring.
研究领域:
分析化学
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环境科学
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纳米生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2015
·
IF 6.409
Dan Wu,Xiang Ren,Lihua Hu,Dawei Fan,Yang Zheng,Qin Wei
A highly sensitive electrochemical adenosine aptasensor was fabricated by covalently immobilizing 3′-NH 2 -terminated capture probe (SSDNA1) and thionine (TH) on Au–GS modified glassy carbon electrode. 3′-SH-terminated adenosine aptamer (SSDNA2) was adsorbed onto palladium/copper alloyed supported on MWCNTs ( [email protected] )-conjugated multiple bienzymes, glucose oxidase (GOx), and horseradish peroxidase (HRP) (SSDNA2/ [email protected] /HRP/GOx). Then, it was immobilized onto the electrode surface through the hybridization between the adenosine aptamer and the capture probe. The signal was amplified based on the gradual electrocatalytic reduction of GOx-generated hydrogen peroxide by the multiple HRP through the mediating ability of the loaded multiple TH. However, the peak current of TH decreased in the presence of adenosine because the interaction between adenosine and its aptamer made SSDNA2/ [email protected] /HRP/GOx release from the modified electrode . Various experimental parameters have been optimized for the detection of adenosine and tests for selectivity , reproducibility and stability have also been performed. Under the optimal condition, the proposed aptasensor displayed a wide linear range (10–400 nM) with the low detection limit (2.5 nM), which has been applied in human serum samples with satisfactory results. Thus, the combination of Au–GS as a sensor platform and [email protected] /HRP/GOx as labels can be a promising amplification strategy for highly sensitive adenosine detection.
研究领域:
生物传感器
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分析化学
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医学检测
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2016
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IF 4.758
Yilin Li,Shupei Zhang,Hong Dai,Zhensheng Hong,Yanyu Lin
This work designed a delicate signal-on photoelectrochemical sensing methodology for concanavalin A monitoring by coupling graphene-supported quasi-octahedral TiO 2 mesocrystal with an efficient enzyme-free labeling strategy. Herein, graphene with prominent conductivity could accelerate photo-generated electrons transfer, thus enhancing the photocurrent response. Currently, labeling of various enzymes was the mainly strategy for signal amplification, unfavorably, this method usually suffered inferior stability. Hence, the exploration and development of NiCo 2 O 4 labeling was proposed here, which could effectively accept the photo-induced electrons from conduction band of TiO 2 mesocrystal, leading to the decrease of the photocurrent intensity. Accordingly, a signal-on non-enzymatic photoelectrochemical sensor was constructed based on immobilized concanavalin A competing with isolated concanavalin A of various concentrations for reaction with the NiCo 2 O 4 labeled glucose conjugates. Under the optimized conditions, the photocurrent increased with the increasing concanavalin A concentration in a dynamic linear range from 0.05 ng/mL to 0.5 μg/mL, thereby proposing a general format for practical use in quantitative determination of concanavalin A. Promisingly, the successful consideration of graphene sensitized TiO 2 mesocrystal further broadened the application of excellent photoactive materials for photoelectrochemical sensing platform.
研究领域:
分析化学
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材料科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2015
·Vol. 8
·期 16
·8553-8558
·
IF 7.394
Lin Fan,Yanyan Tian,Rong Yin,Doudou Lou,Xizhi Zhang,Meng Wang,Ming Ma,Shouhua Luo,Suyi Li,Ning Gu,Yu Zhang
Conventional immunohistochemistry is limited to subjective judgment based on human experience and thus it is clinically required to develop a quantitative immunohistochemical detection. 3,3′-Diaminobenzidin (DAB) aggregates, a type of staining product formed by conventional immunohistochemistry, were found to have a special optical property of dark-field imaging for the first time, and the mechanism was explored. On this basis, a novel immunohistochemical method based on dark-field imaging for detecting HER2 overexpressed in breast cancer was established, and the quantitative analysis standard and relevant software for measuring the scattering intensity was developed. In order to achieve a more sensitive detection, the HRP (horseradish peroxidase)-labeled secondary antibodies conjugated gold nanoparticles were constructed as nanoprobes to load more HRP enzymes, resulting in an enhanced DAB deposition as a dark-field label. Simultaneously, gold nanoparticles also act as a synergistically enhanced agent due to their mimicry of enzyme catalysis and dark-field scattering properties.
研究领域:
医学影像
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癌症研究
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生物化学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
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2019
·
IF 2.507
Huijuan Yang,Candace S. Bever,Huiyan Zhang,Ghulam Mujtaba Mari,Hongfang Li,Xiya Zhang,Liuchuan Guo,Ziwen Wang,Pengjie Luo,Zhanhui Wang
Enzyme labeling of an antigen or an antibody helps to visualize and amplify the signal and is an important reagent used in immunoassays for the detection of a target of interest. In this research, soybean peroxidase (SBP), a less commonly used enzyme reporter, was compared in immunoassays with the two most commonly used reagents, horseradish peroxidase (HRP) and alkaline phosphatase (ALP). The enzyme-antibody conjugates were evaluated by their performance in an indirect competitive enzyme-linked immunosorbent assay (icELISA) and in an indirect competitive chemiluminescent enzyme immunoassay (icCLEIA) for ractopamine (RAC). The results revealed that the more affordable SBP offers a long-lasting chemiluminescent signal, which outperformed ALP and HRP. SBP-antibody conjugate (SBP-Ab) based immunoassays produced lower limits of detection (LODs) and better accuracy in the detection of RAC in animal urine samples. Additionally, SBP-Ab has advantages in being more resistant to heat, acid and organic solvents . These results suggest that SBP could be a potentially excellent alternative to HRP and ALP for the development of immunoassay in food safety field.
研究领域:
生物化学
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分析化学
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食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）