Nanoscale
·
2020
·Vol. 12
·期 46
·23578-23585
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IF 6.895
Jinxing Chen,Qian Ma,Minghua Li,Weiwei Wu,Liang Huang,Ling Liu,Youxing Fang,Shaojun Dong
Although nanozymes overcome a series of shortcomings of natural enzymes, their wide applications are hampered due to their limited varieties. In this work, we propose a coenzyme-dependent nanozyme, a synergistic composite comprising zeolitic imidazolate frameworks encapsulated with polyethylenimine (PEI) and functionalized with a flavin mononucleotide (PEI/ZIF-FMN). The flavin mononucleotide (FMN) plays the role of a prosthetic group, and the positively charged NH2 groups in PEI readily provide the binding affinity to nicotinamide adenine dinucleotide (NADH), which facilitates the electron transfer from NADH to FMN and terminal electron acceptors (such as O2) with a greatly enhanced (80 times) catalytic performance. The integrated nanoparticle–coenzyme composite works as an NADH oxidase mimic and couples with dehydrogenases for the tandem enzymatic reaction. PEI/ZIF-FMN also mediated the electron transfer from NADH to cytochrome c (Cyt c), thereby exhibiting Cyt c reductase-like activity.
研究领域:
生物化学
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纳米生物学
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DOI
PubMed
高影响因子
ANALYST
·
2012
·Vol. 137
·期 19
·4552-4558
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IF 4.23
Xing Liu,Qi Wang,Huihui Zhao,Lichun Zhang,Yingying Su,Yi Lv
Inorganic nanomaterials that mimic enzymes are fascinating as they potentially have improved properties relative to native enzymes, such as greater resistance to extremes of pH and temperature and lower sensitivity to proteases. Although many artificial enzymes have been investigated, searching for highly-efficient and stable catalysts is still of great interest. In this paper, we first demonstrated that bovine serum albumin (BSA)-stabilized MnO2 nanoparticles (NPs) exhibited highly peroxidase-, oxidase-, and catalase-like activities. The activities of the BSA-MnO2 NPs were evaluated using the typical horseradish peroxidase (HRP) substrates o-phenylenediamine (OPD) and 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of either hydrogen peroxide or dissolved oxygen. These small-sized BSA-MnO2 NPs with good dispersion, solubility and biocompatibility exhibited typical Michaelis–Menten kinetics and high affinity for H2O2, OPD and TMB, indicating that BSA-MnO2 NPs can be used as satisfactory enzyme mimics. Based on these findings, BSA-MnO2 NPs were used as colorimetric immunoassay tags for the detection of goat anti-human IgG in place of HRP. The colorimetric immunoassay using BSA-MnO2 NPs has the advantages of being fast, robust, inexpensive, easily prepared and with no HRP and H2O2 being needed. These water-soluble BSA-MnO2 NPs may have promising potential applications in biotechnology, bioassays, and biomedicine.
研究领域:
材料科学
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生物化学
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生物技术
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纳米技术
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Jiabi Xu,Yanyan Xing,Yutong Liu,Mingzhe Liu,Xiaohong Hou
In recent decades, artificial nanozymes with excellent stability, low cost and availability have been gradually explored to avoid the limits of natural enzymes such as poor stability, high cost and difficult preparation. Herein, for the first time, we investigated the capability of nanoscale Fe 3 O 4 @MIL-100(Fe) as a nanozyme, which was quickly synthesized in situ by a microwave-assisted method within 20 min using Fe 3 O 4 as the metal precursor. The obtained Fe 3 O 4 @MIL-100(Fe) showed satisfactory intrinsic dual enzyme mimetic activities, including peroxidase (POD)- and catalase (CAT)-like activities. Moreover, a simple and effective colorimetric biosensor was fabricated to detect glutathione (GSH) based on its POD-like activity. The proposed measurement had a linear range of 1–45 μM and a limit of detection (LOD) of 0.26 μM (3.3 δ/S). It was proved that the established colorimetric sensing system could be successfully applied to detect GSH in actual biological samples. Importantly, the outstanding reusability and stability made it extremely valuable as a catalyst. The present work implied that Fe 3 O 4 @MIL-100(Fe) synthesized in situ by the microwave-assisted method was a very promising candidate for biocatalyst and biosensing.
研究领域:
纳米生物技术
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分析化学
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生物传感
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酶模拟
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分子诊断
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转化医学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Wen Jiang,Jia Zheng,Jian Su,Yue Tang,Yuangen Wu,Yating Cao,Wentao Cao
Colorimetric methods have the benefits of rapid detection and easy operation in dimethoate (DMT) analysis. However, the existing colorimetric sensor for DMT detection still face challenges in terms of performance stability and result reliability of sensing materials. Bimetallic Ag 2 CrO 4 nanoparticles (NPs) have been found to have both oxidase-mimic and laccase-mimic catalytic activities. DMT has opposite effect on the above dual-enzyme-mimic activities, which can enhance oxidase-mimic activity while inhibiting laccase-mimic activity of Ag 2 CrO 4 NPs. Therefore, a novel colorimetric sensor was constructed using the dual-enzyme-mimic activities as a sensing signal for rapidly detecting DMT in vegetables. The concentration of DMT is directly correlated with the absorbance change of the sensing solution, and the color change is further integrated with the smartphone to enable quantitative measurement of DMT. The limits of detection were as low as 8.7 μg L −1 in the oxidase-mimic channel and 10.9 μg L −1 in laccase-mimic channels. Besides, the colorimetric sensor has shown marked preference in selectivity over other competing pesticides, and obtained relatively preferable recovery rates in some vegetables, indicating that the established sensor has a wide range of potential applications in the area of vegetable pesticide detection.
研究领域:
分析化学
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食品安全
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纳米技术
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农药检测
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2025
·
IF 3.9
Changjin Zhou,Jiahao Zhang,Hongyi Li,Dong Shao,Junlun Zhu,Wei Wen,Xiuhua Zhang,Zhengfang Tian,Shengfu Wang
As a key technology enabling green and sustainable chemical processes, nanozymes have significantly enhanced their catalytic efficiency and economic viability. Therefore, the development of a higher catalytic activity enzyme mimic to construct a portable and easily sensing strategy for sensitive, rapid, and convenient determination of a specific target is exceedingly elemental for an advanced sensing platform. Herein, we designed and prepared copper-doped palladium metallene (Cu–Pd metallene) with multiple enzyme-like activities for a sensitive colorimetric assay. The relevant kinetic parameters of Cu–Pd metallene with three kinds of enzyme-like activities were evaluated. The Michaelis–Menten constant of laccase mimic and oxidase mimic were calculated to be 0.041 and 0.51 mM, which exhibited high affinity to 2,4-dichlorophenol and 3,3′,5,5′-tetramethylbenzidine in the corresponding system, respectively. Inspired by its high catalytic activity, a simple and portable colorimetric sensing platform based on Cu–Pd metallene was developed to detect and distinguish epinephrine, phenolics, and thiram by UV–vis absorption spectrum and smartphone. Among them, the proposed method can detect epinephrine concentration in the range of 1–15 μg mL–1 by laccase-like activity, and a calculated reliable limit of detection is 0.18 μg mL–1. Owing to the inhibitory effect, the colorimetric sensing platform was used to analyze thiram, which presented a concentration range of 10–500 ng mL–1 and an acceptable detection limit of 4.4 ng mL–1. Considering the advantages of accuracy and ease of use of Cu–Pd metallene-based colorimetric sensing platform, it has great potential for real-time monitoring of relevant targets in poor areas.
研究领域:
纳米技术
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分析化学
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生物传感器
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酶模拟
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环境监测
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食品安全
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即时检测
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2025
·
IF 5.1
Yuxian Zhao,Wenxu Wang,Yu Bai,Huaixiao Geng,Aoxue Tan,Xiaodan He,Shuang Han,Jine Wang,Yuewu Zhao
Nanozymes have emerged as promising alternatives to natural enzymes owing to their high stability, cost-effectiveness, and catalytic versatility. In this work, the Ce-TPA metal-organic frameworks (MOF) material with dual enzyme activities of oxidase (OXD) and peroxidase (POD) was constructed through a bottom-up approach using the coordination reaction between Ce ions and terephthalic acid (TPA) molecules. The formation of MOF structure not only displays high water adsorption capacity but also enhances the thermal stability of TPA organic ligands, which also lays the foundation for the reuse of Ce-TPA materials. Therefore, the Ce-TPA MOF materials can produce high adsorption capacity for methyl blue (MB) through electrostatic attraction and hydrogen bonds, and this adsorption process follows the pseudo-second-order and intraparticle diffusion model. We also compared the effects of synchronous and asynchronous removal of MB, and the results confirmed that combining the pre-adsorption process with enzymatic degradation can enhance the removal efficiency of MB, with an efficiency exceeding 95 %. Additionally, steady-state enzymatic kinetics demonstrated that the Ce-TPA MOF materials has a high affinity for the MB substrate, following the Michaelis-Menten behavior, and the generated superoxide anions (O 2 •− ) and hydroxyl radicals (•OH) are the main reasons for the oxidation and degradation of MB dyes.
研究领域:
环境化学
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纳米生物技术
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催化化学
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环境功能材料
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污染控制
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2025
·
IF 5.7
Lili Liu,Wenting Ye,Yanqiu Duan,Xinran Song,Li Ding,Yu Chen,Meiqi Chang
Inflammatory bowel disease (IBD) is a chronic inflammatory disorder marked by dysregulated immune responses and pyroptosis of intestinal epithelial cells, a type of programmed cell death that aggravates inflammation. Current therapeutic strategies frequently encounter limitations in both efficacy and specificity. Herein, we developed a calcium manganese-based (CaMn2O4) pancatalytic nanozyme designed to mitigate pyroptosis and alleviate IBD symptoms. The calcium manganese-based nanozyme exhibits dual enzyme (catalase and superoxide dismutase)-mimetic catalytic activities, scavenging reactive oxygen species and suppressing GSDMD cleavage, a key mediator of pyroptosis. In vitro studies demonstrated that CaMn2O4 nanozyme significantly reduced pyroptotic cell death in lipopolysaccharide/nigericin-stimulated cells. In a murine colitis model, CaMn2O4-based nanozyme treatment attenuated inflammatory infiltration, preserved epithelial barrier integrity, and downregulated pyroptosis-related markers. This study highlights the potential of pancatalytic nanotherapy targeting pyroptosis as a novel strategy for IBD treatment.
研究领域:
纳米医学
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免疫学
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胃肠病学
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生物无机化学
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酶学
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DOI
PubMed
高影响因子
Biomaterials Science
·
2021
·Vol. 9
·期 7
·2647-2657
·
IF 6.843
Xiaojuan Wang,Xuejian Zhou,Kai Yang,Qiong Li,Rongxin Wan,Guoying Hu,Jin Ye,Yue Zhang,Jiahui He,Hanqing Gu,Yongfang Yang,Lei Zhu
In this study, UiO-66-NH2 metal–organic framework (MOF) nanoparticles with peroxidase and oxidase mimetic activities were incorporated into a chitosan (CS) matrix by a simple and environmentally friendly method. The UiO-66-NH2/CS composite membrane possesses the peroxidase mimicking activity in the presence of traces of H2O2, thus resulting in good antibacterial properties. Intriguingly, 30 min of UV pre-irradiation of the UiO-66-NH2/CS composite membrane, in the absence of H2O2, still leads to a good antibacterial activity. This was attributed to the oxidase mimetic activity and the peroxidase mimicking activity of UiO-66-NH2. In such a way, the side effects of direct exposure to UV irradiation and H2O2 can be avoided for wound-healing treatments. The antibacterial mechanism was further proved by antibacterial experiments, TMB·2HCl color development experiments, reactive oxygen species generation tests and electron spin resonance tests. As a potential medical antibacterial dressing, in vitro membranes were also investigated.
研究领域:
材料科学
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纳米技术
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生物材料
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2025
·
IF 4.7
Xueyu Wang,Zhuojun Yan,Mengmeng Yao,Xiuqiang Li,Zhongming Zhao,Cheng Cao,Zhijie Zhao,Fanglian Yao,Yuping Wei,Hong Zhang,Junjie Li
Blood-contacting devices serve as a mainstay in clinical treatment, yet thrombosis remains a major cause of device failure and poses risks to patient health. In this study, we developed a diselenide cross-linker, N,N′-bis(methacryloyl)selenocystamine (BMASC), incorporated into poly(sulfobetaine methacrylate) (PSBMA) microgels (defined as BSM) to create an enzyme-mimetic zwitterionic microgel coating (BSMC). The superhydrophilicity of PSBMA provides outstanding antifouling performance, while the diselenide bonds mimic the catalytic action of glutathione peroxidase (GPx) in generating nitric oxide (NO). The microgels are covalently anchored to substrates pretreated with polydopamine (PDA) and polyethylenimine (PEI) through an epoxy-amine ring-opening reaction. During the drying process, the interpenetrating PSBMA chains of the microgels diffuse, forming a dense and smooth hydrogel coating. The BSMC exhibits exceptional resistance to nonspecific adhesion of proteins, cells, and bacteria, with the synergistic effects of antifouling properties and NO effectively inhibiting platelet adhesion. Furthermore, rabbit blood circulation experiments demonstrate the superior antithrombotic efficacy of the BSMC. This coating holds promise as an effective solution to address the thrombus formation challenges of blood-contacting devices.
研究领域:
生物材料
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医学工程
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药物化学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2024
·
IF 4.9
Miaomiao Song,Weihua Ma
Increasing attention has been directed towards oxidase mimics due to their catalytic ability toward enzyme substrates in the absence of H 2 O 2 , offering great colorimetric sensing potential in assays and diagnostics. However, slow electron-transfer and unstable absorbance over time often lead to unsatisfactory enzymatic reaction rates and unreliable readouts, significantly reducing detection efficiency and accuracy. Herein, we report on an Ag 6 Si 2 O 7 nanozyme utilizing 3,3′,5,5′-tetramethylbenzidine (TMB) as a chromogenic substrate, demonstrating excellent oxidase-like activity and long-term stable colorimetric signals. Experimental results reveal that the excellent catalytic kinetics of Ag 6 Si 2 O 7 (K m = 0.197 mM, Vmax = 5.1 × 10 −7 M/s) are attributed to ultrafast electron transfer between Ag + and TMB, mediated by Ag 2 O intermediates formed from self-released alkaline ions. Additionally, self-released H 2 SiO 4 2− improves the stability of the oxidized substrate, resulting in a long-term stable colorimetric signal (∼20 min), which exhibits reliable accuracy regardless of the time point at which analyte is added within 20 min, thus eliminating the interference of individual operation time errors. Ascorbic acid (AA) is selected as the target molecule to assess the performance of the Ag 6 Si 2 O 7 -TMB colorimetric sensing system. This approach demonstrates a wide detection range from 5 to 300 μM and a detection limit of 0.452 μM. The developed colorimetric sensing strategy exhibits good stability, specificity, rapidity, and potential application in real sample detection.
研究领域:
生物化学
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分析化学
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医学诊断
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xiaoyu Zheng,Lin Xing,Xuemei Zhou,Yulian Tang,Zhen Liu,Xueting Zhang,Lei Hu,Zhengquan Yan
To monitor toxic NO 2 − and S 2− conveniently and effectively in 100% aqueous, nano CeO 2 was identified and modified first onto 2-dimensional Co 3 O 4 substrate (CeO 2 @2D Co 3 O 4 ) under microwave-assisted conditions at 140 °C. The obtained CeO 2 @2D Co 3 O 4 expressed excellent stability and superior oxidase-mimic activity with a wide temperature range from 20 to 60 °C. Under the optimized conditions (pH 4.0, 25 °C, 0.07 mM TMB), CeO 2 @2D Co 3 O 4 could accelerate the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to its blue oxidation (oxTMB) within 12 min at room temperature , whose Michaelis constant ( K m ) and maximal reaction rate ( v max ) were 0.017 mmol/L and 2.17 × 10 −7 mol/L·s –1 respectively. Especially, both NO 2 − and S 2− could exclusively make the blue CeO 2 @2D Co 3 O 4 -TMB system into colorless and green respectively, expressing significant visibility. When applied for visual detection of NO 2 − and S 2− in real water, soil, drink and food samples, the recoveries were 97.6~104.6% and 97.5~104.1% with low detection limits of 3.26 × 10 −9 mol/L and 6.65 × 10 −8 mol/L (S/N = 3), far below the maxima in drinking water permitted by World Health Organization (WHO). The strengthened oxidase-mimic activity of CeO 2 @2D Co 3 O 4 and its different recognition mechanisms to NO 2 − and S 2− were studied in detail.
研究领域:
环境科学
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生物化学
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材料科学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2021
·
IF 4.098
Yanyun Zou,Qingli Chai,Ting Zhu,Xiaoxiao Yu,Guobin Mao,Ningxing Li,Jinyang Chen,Guosong Lai
In this work, an environmentally-friendly and cost-effective enzyme mimic was obtained by facile one-pot preparation of chitosan/Cu/Fe (CS/Cu/Fe) composite. This composite exhibited significantly enhanced oxidase-mimicking activity during catalyzing the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB). The CS/Cu/Fe composite was comprehensively characterized and the possible catalytic mechanism was reasonably explored and discussed. Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic. Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg 2+ ), the rapid colorimetric detection of Hg 2+ was successfully carried out in the analytical tube. This colorimetric method showed good linear response to Hg 2+ over the range from 40 nM to 8.0 μM with a detection limit of 8.9 nM. The method also revealed high selectivity and satisfactory results in recovery experiments of Hg 2+ detection in tap water and lake water. Furthermore, it was found that the effective removal of Hg 2+ could be realized in the analytical tube based on efficient Hg 2+ adsorption by CS/Cu/Fe composite and agarose hydrogel. This study not only prepared a robust and low-cost enzyme mimic, but also proposed a smart strategy to simultaneously monitor and remove toxic Hg 2+ from contaminated water.
研究领域:
环境科学
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分析化学
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材料科学
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水处理
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DOI
PubMed
高影响因子
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