BIORESOURCE TECHNOLOGY
·
2015
·
IF 4.494
Xuliang Lin,Xueqing Qiu,Duming Zhu,Zihao Li,Ningxin Zhan,Jieyi Zheng,Hongming Lou,Mingsong Zhou,Dongjie Yang
Effect of the molecular structure of lignin-based polyoxyethylene ether (EHL–PEG) on enzymatic hydrolysis of Avicel and corn stover was investigated. With the increase of PEG contents and molecular weight of EHL–PEG, glucose yield of corn stover increased. EHL–PEG enhanced enzymatic hydrolysis of corn stover significantly at buffer pH 4.8–5.5. Glucose yield of corn stover at 20% solid content increased from 32.8% to 63.8% by adding EHL–PEG, while that with PEG4600 was 54.2%. Effect of EHL–PEG on enzymatic hydrolysis kinetics of cellulose film was studied by quartz crystal microbalance with dissipation monitoring (QCM-D) and atomic force microscopy (AFM). An enhancing mechanism of EHL–PEG on enzymatic hydrolysis kinetics of cellulose was proposed. Cellulase aggregates dispersed by EHL–PEG excavated extensive cavities into the surface of cellulose film, making the film become more loose and exposed. After the maximum enzymatic hydrolysis rate, the film was mainly peeled off layer by layer until equilibrium.
研究领域:
生物质转化
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酶工程
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高分子化学
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表面科学
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可再生能源
✓ 已验证
DOI
高影响因子
CELLULOSE
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2019
·Vol. 26
·期 6
·3801-3814
·
IF 3.917
Liu Jing,Hu Huichao,Gong Zhenggang,Yang Guangxu,Li Runqing,Chen Lihui,Huang Liulian,Luo Xiaolin
Abstract Based on Hildebrand solubility parameter (δ H ) theory, a novel organosolv pretreatment medium (OPM), 1-pentanol (80%, v/v)–water mixture with a δ H value of 27.4 (J/cm 3 ) −1/2 , was discovered. This OPM achieved near-complete removal of non-cellulosic components (hemicellulose 96.2% and lignin 87.8%) from bamboo under mild conditions [130 °C, 20 min, 4% (w/w) H 2 SO 4 ]. For the OPMs with similar δ H values [DMSO (80%, v/v)- and 1-pentanol (80%, v/v)-water, 30.9 vs. 27.4 (J/cm 3 ) −1/2 ] to that of bamboo lignin [28.3 (J/cm 3 ) −1/2 ] used in this study, the effect of the δ h value (contribution of hydrogen-binding force to the δ H ) on lignin removal was also discussed. After overcoming the negative effect of lignin deposits on enzymes with biocompatible soy protein (SP), a decent cellulose enzymatic conversion (EC) of 92.6% was achieved by 1-pentanol (80%, v/v)-water induced organosolv pretreatment at a low cellulase loading (5 FPU/g glucan). This cellulose EC was much higher than those of other OPMs (< 63%) with the δ H values very different from that of bamboo lignin. Integration of organosolv pretreatment with an ideal OPM and enzymatic hydrolysis using SP as an additive can be thereby viewed as a viable way to achieve the robust cellulose EC at low enzyme loading, which shows great potential for improving the economics of organosolv pretreatment-based lignocellulose biorefinery. Graphical abstract
研究领域:
生物能源
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植物学
✓ 已验证
产品关联
DOI
高影响因子
BOTANICA MARINA
·
2024
·Vol. 67
·期 5
·487-511
·
IF 1.6
Matthew N. Noach,Richard L. Mampana,Eugene Van Rensburg,Neill J. Goosen,Robert W. M. Pott
Alginate is a brown seaweed-based linear polysaccharide of D – mannuronic acid and L – guluronic acid residues. Hydrolysis products of the polysaccharide, specifically oligosaccharides, have been receiving increasing interest, due to their significant bioactivity and potential utilisation routes. The bioactivity of alginate oligosaccharides is closely linked to structural characteristics, namely: molecular weight, degree of polymerisation, and ratio of the monomers (the M/G ratio). Hence, potential applications (such as utilisation as a biostimulant fertilizer) depend on these parameters. This review focuses on recent advances in producing alginate oligosaccharides using chemical or enzymatic methods. The literature survey includes utilisation of these methods at both laboratory and industrial scale. For the chemical methods, we assessed the standard laboratory scale procedures of alginate oligosaccharide production, the potential of scaling up to an industrial level, and the subsequent challenges. For the enzymatic route, we provide an overview of alginate lyases and the application perspectives of enzymatic hydrolysis of alginate.
研究领域:
海洋生物学
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生物化学
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酶工程
✓ 已验证
DOI
高影响因子
Bioresource Technology Reports
·
2019
·
IF 0
Yingxue Gong,Zhibin Fu,Menglian Liu,Yu Dai,Jianghai Lin,Zehuan Liu
The alkalis sodium ethoxide and calcium hydroxide were combined to pretreat the abundant agricultural waste sugarcane leaf (SCL). The pretreatment conditions were optimized using single factors and orthogonal array, and the results of material's chemical compositions and enzymatic hydrolysis were investigated. The optimized conditions were pretreating SCL with C 2 H 5 ONa:Ca(OH) 2 volume ratio of 4:1 at 121 °C for 30 min. Under these conditions, the lignin removal rate of SCL was 86.26%, while the total reducing sugars and total enzymatic efficiency of enzymatic hydrolysis could reach 14.82 g/L and 80.02% respectively. These pretreating and hydrolysis results were similar compared with those of SCL pretreated with strong base C 2 H 5 ONa alone. But the strong base dosage was saved 20% and the pretreatment time was shortened to 30 min. Therefore the combined alkali pretreatment could significantly save energy and reduce environmental pollution, which made it a promising method in large-scale production.
研究领域:
生物能源
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农业废弃物利用
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生物质转化
✓ 已验证
DOI
Green Energy & Environment
·
2025
·
IF 14.6
Jie Liang,Jingcong Xie,Jianchun Jiang,Yan Ma,Jun Ye,Xianhai Zeng,Kui Wang
A novel environmentally benign biphasic system composed of propylene carbonate (PC) and aqueous solution of p -toluenesulfonic acid ( p -TsOH aq) was designed for the efficient valorization of lignocellulosic bamboo residues, resulting in more than 95.5% of hemicellulose and 97.2% of lignin digested under mild conditions of 130 °C for 1 h. Meanwhile, 91.9% of cellulose was retained with loose structure, followed by 95.8% enzyme hydrolysis yield and 347.9 mg/g of glucose yield. Notably, the synergistic effect between PC and p -TsOH on efficiency and selectivity was proposed by a control group experiment and subsequently verified, which is believed to be responsible for the simultaneous degradation and separation of lignin and hemicelluloses into oligomeric phenols and pentose, also facilitating subsequent valorization. Furthermore, the novel PC/ p -TsOH aq biphasic system demonstrated excellent retrievability and adaptability to different feedstocks, offering a promising green strategy for the efficient valorization of lignocellulosic biomass in industrial biorefineries.
研究领域:
生物质能
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绿色化学
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环境科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Wen Tan,Qinjun Zhang,Pin Chen,Qingqing Sun,Changqing Wei,Xinwen Xu,Natthawuddhi Donlao,Jinhu Tian
To investigate the impact of safflower seed oil on the structural and digestive properties of complexes formed by fatty acids of varying chain lengths with maize starch, the starch-fatty acid ternary complexes were prepared by a hydrothermal method. The results indicated that safflower seed oil inhibited the complexation of relatively short-chain fatty acids (C10:0, C12:0, and C16:0) with starch, and promoted the complexation of long-chain fatty acids (C18:0). Intriguingly, safflower seed oil showed no significant impact on the formation of linoleic acid (C18:2) complexes, suggesting selective interactions within the starch-fatty acid complexes. In addition, the addition of safflower seed oil did not affect the thermal stability of the complexes, but significantly improved the anti-digestibility properties of the starch-complexes in each group, with the RS content reaching 59.08 % in the C16:0 group. In conclusion, this study provides insights for the development of high-quality resist starch-lipid ternary complexes.
研究领域:
食品科学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Kai Lin,Ao Xia,Yu Yang,Yun Huang,Xianqing Zhu,Xun Zhu,Kaiyong Cai,Zidong Wei,Qiang Liao
Pretreatment is critical to promote enzymatic hydrolysis for the production of lignocellulose-based biofuels. In this study, inspired by the lignin degradation through the mouthpart and symbiotic fungi of termite, a synergistic biomass pretreatment system using ball milling and Fenton-like reactions was first proposed. Ball milling can reduce the particle size, while Fenton-like reactions can modify the lignin and cause pore structures, leading to a 44.2% increment in total pore volume. In addition, lignin-based aromatic aldehydes can be produced with a 67.1% increase in yield over no-ball milling conditions, which can be used as natural mediators. More importantly, under the optimal pretreatment conditions (1.5% H2O2, 1 mg L–1 Fe-TAML catalyst, 300 rpm, and 12 h), the highest initial glucose productivity was 2.83 g L–1 h–1, which is 2.4 times higher than untreated samples. The conversion of cellulose in 24 h is enhanced by 62.3% with half of the enzyme input when compared with untreated wheat straw. Thus, the synergy of ball milling and Fenton-like reactions helps to promote lignin depolymerization and enzymatic hydrolysis of lignocellulose.
研究领域:
生物能源
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
Food & Function
·
2021
·Vol. 13
·期 2
·880-890
·
IF 5.396
Yana Zhao,Huizhen Li,Zhijun Zhang,Zhiqing Ren,Fuhan Yang
Polyphenols exhibit potential functional activities, especially rosmarinic acid (RosA) and caffeic acid (CafA). In this study, two different methods, ultrasonic-assisted ethanol extraction (60%) and ultrasound-assisted cellulase (≥15 000 Ug−1, 2%) hydrolysis, were used for the extraction of the total phenolics from 44 species of Perilla frutescens. The Folin–Ciocalteu method of detection showed that the content of the total phenolics extracted by cellulase hydrolysis was the highest and attained up to 28.00 mgGAE per gextracts for ZB1. Continuously, the extracts were purified using XDA-8 macroporous resin and medium-pressure liquid chromatography (MPLC), and the content of the total phenolics improved to 66.62 mgGAE per gextract. A high-performance liquid chromatography (HPLC) assay showed that the total polyphenols were mainly composed of gallic acid, caffeic acid, rosmarinic acid, luteolin and apigenin. Besides, a sequential XDA-8 macroporous resin combined with high-speed counter-current chromatography (HSCCC)/MPLC system was established for the simultaneous isolation and preparation of RosA (purity 98.29%) and CafA (purity 97.01%) from the extracts. Furthermore, the antibacterial activities of the total polyphenols were evaluated by the disc diffusion method and scanning electron microscopy (SEM) observation. The results verified that the total polyphenols had effective antibacterial activity on three kinds of bacteria including E. coli, S. aureus, and B. subtilis in a concentration-dependent manner. All of these results demonstrated that the ultrasound-assisted cellulase hydrolysis extraction of the total polyphenols and the proposed three-step separation of RosA and CafA gave high yields and good purity, and they exhibited effective antibacterial ability.
研究领域:
植物化学
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天然产物研究
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Kailin Ye,Jiaming Qi,Yang Zhang,Jinmei Wang,Jian Guo,Xiaoquan Yang
Salt reduction strategies in liquid foods remains a significant challenge. The oral perception of Na + is strongly dependent on its salivary penetration and mucosal adsorption. To understand the mechanism of Na + saltiness enhancement modulated by polysaccharide , protease hydrolysis (trypsin and acid protease) was used to modify the molecular conformation of Gum Arabic (GA), a highly branched polyanionic polysaccharide . The salivary permeation, mucosal retention in mucin layer, and salty taste of Na + were investigated. Saltiness-enhanced effect of GA is strongly influenced by its molecular conformation. Compared to native GA, protease-hydrolyzed GA exhibited a higher saltiness-enhanced effect due to more rapid Na + diffusion in saliva and stronger mucosal retention. Protease-hydrolyzed GA, especially acid protease-hydrolyzed GA (GA-AcPr), exhibited lower zeta potential (from original −22.5 to −27.96) and stronger Na + loading capacity. The obvious decreases in molecular weight (M w ) and the radius of gyration ( R g ) were observed for trypsin-hydrolyzed GA (GA-Trypsin), which may be responsible for the higher Na + diffusion effect of GA-saliva solution due to decreased molecule twine around each other. GA-AcPr exhibited looser and highly branched conformation, as evidenced by a more pronounced "U-shaped" curve and higher R g /R h . Moreover, Quartz crystal microbalance with dissipation (QCM-D) results verified stronger affinity between GA-AcPr and mucin, indicating that the saltiness-enhanced effect of GA-AcPr may be mainly achieved by increasing adhesive retention of Na + . These results would be useful to better understand the structure-function relationship of polysaccharides and the taste perception of Na + .
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Jun-Yi Yin,Lu-Yao Ma,Ming-Yong Xie,Shao-Ping Nie,Jian-Yong Wu
Konjac glucomannan (KGM) with a molecular weight (MW) of 823.4 kDa was partially degraded by endo-1,4-β-mannanase. Two hydrolyzed KGM fractions (KGM-M-1: 147.2 kDa and KGM-M-2: 21.5 kDa) were characterized and applied to the animal tests in comparison with the native KGM. After oral feeding to the mice, KGM-M-1 and KGM-M-2 significantly increased the levels of short chain fatty acids (SCFAs) in the colonic contents and the native KGM increased the SCFAs in the cecum. The more significant effect of the native KGM in the cecum may be attributable to its high viscosity, slowing down the movement of intestinal microflora through the cecum, while the lower MW KGM-M-1 and KGM-M-2 could move more easily through the colon to be fermented by colonic bacteria. This new finding may be useful for future research and development of low-MW KGM polysaccharides through enzyme hydrolysis for the desired gut health benefits.
研究领域:
食品科学
·
营养学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Liqin Liu,Xingye An,Hao Zhang,Zonghong Lu,Shuangxi Nie,Haibing Cao,Qingliang Xu,Hongbin Liu
Starch based bio-latex has been widely researched in the coating paper area for the purpose of partial replacement of petroleum-based binders. In this paper, a green and facile ball milling pretreatment was proposed to modify the starch granules before α-amylase hydrolysis by breaking up their crystalline structure, thus improving the accessibility and susceptibility of amylase into starch structure. It was found that the improved hydrolysis process after 8 h ball milling can generate suitable degree of polymerization of polysaccharides or oligosaccharides, which further facilitated the following H 2 O 2 oxidation and SHMP crosslinking processes. In addition, a mechanism was also demonstrated to illustrate the improvement induced by ball milling pretreatment. The prepared bio-latex with crosslinking-structure performed excellent adhesive properties when substituted 25 % of petroleum-based latex during paper coating application, which showed great potential in improving the economic, cost, and environment benefits of traditional production of coated paper.
研究领域:
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Xuliang Lin,Linjun Wu,Siqi Huang,Yanlin Qin,Xueqing Qiu,Hongming Lou
The origin, amount, hydrophilicity, charge, molecular weight and its distribution of lignin have significant influences on the enzymatic hydrolysis of lignocellulose. The enzymatic hydrolysis of lignocellulose was essentially enhanced by lignin-based polyoxyethylene ether (EHL-PEG), whereafter followed by PEG4600 and lignosulfonate (LS). The effect of LS, EHL-PEG and PEG4600 on the adsorption and enzymatic hydrolysis kinetics of cellulase on the gold surface and cellulose film was investigated by Quartz Crystal Microbalance with dissipation monitoring (QCM-D). Results showed that the interaction of LS or EHL-PEG with cellulase was electrostatic attractive and hydrophobic effect, respectively, and formed hydrophilic cellulase aggregates. LS-Cellulase peeled off the cellulose film layer by layer, while the hydrophobic phenylpropane structure of EHL-PEG-Cellulase acted as a cellulose binding domain to hydrolysis cellulose through “Hollow” effect and made cellulose become more loose and swollen. At last, a strategy to enhance the enzymatic hydrolysis of lignocellulose by lignin-based amphiphilic polymers was proposed as well.
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）