JOURNAL OF BIOSCIENCE AND BIOENGINEERING
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2019
·
IF 2.032
Li Peng,Qin Ye,Xiaoying Liu,Shulai Liu,Xianghe Meng
Aqueous enzymatic extraction of Camellia sinensis oil was studied. The results suggested that saponin removal pretreatment assisted by ultrasound was effective in decreasing emulsification and in enhancing the free oil recovery. After 70% isopropanol extraction for 30 min under ultrasound, the residue of C. sinensis seeds was further hydrolyzed with free cellulase and Alcalase for 5 h, and calcium ions were concurrently added during enzymatic hydrolysis ( n Ca 2+ : n saponin = 1:2), and free oil recovery up to 94.14% was obtained. Separate immobilization and co-immobilization of Alcalase and cellulase were performed by alginate entrapment combined with glutaraldehyde crosslinking. Specific activity and recovery of activity for Alcalase and cellulase were acceptable. After immobilization, Alcalase and cellulase exhibited higher activity at a wider pH and temperature range. Reuse experiments of immobilized enzymes were conducted. The deactivation kinetics immobilized enzymes were simulated and half-life of immobilized enzyme was estimated. The results indicated that a magnetic supporter facilitated the recovery of immobilized enzymes from tea seed slurry, and that immobilized Alcalase and cellulase had good reusability.
研究领域:
食品科学
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生物技术
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DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2024
·
IF 8.7
Wang Zhang,Jiaye Yu,Donghua Wang,Xiaoyu Han,Tong Wang,Dianyu Yu
In this study, ultrasonic-ethanol pretreatment combined with AEE was developed for oil extraction from hemp seeds. The oil yield reached a maximum of 23.32 % at 200 W ultrasonic power and 30 min ultrasonic time, at this point, the degradation rate of Δ 9 -THC was 83.11 %. By determining the composition of hemp seed before and after pretreatment, it was shown that ultrasonic-ethanol pretreatment reduced the protein content of the raw material. An enzyme mixture consisting of pectinase and hemicellulase (1/1/1, w/w/w) was experimentally determined to be used, and the AEE extraction conditions were optimized using the Plackett-Burman design and the Box-Behnken. The optimal conditions were determined to be pH 5, total enzyme activity of 37,800 U/g, liquid–solid ratio of 10.4 mL/g, enzyme digestion temperature of 32 °C, enzymatic time of 189 min, and oil recovery of 88.38 %. The results of confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) showed that the emulsion formed during ultrasonic ethanol pretreatment was not uniformly distributed, and the droplets appeared to be aggregated; and the irregular pores of hemp seed increased after pretreatment. The contents of Δ 9 -THC and CBN in the extracted oil samples were 9.58 mg/kg and 52.45 mg/kg, respectively. Compared with the oil extracted by Soxhlet extraction (SE), the oil extracted by this experimental method was of better quality and similar in fatty acid composition.
研究领域:
食品科学
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植物化学
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生物工程
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DOI
PubMed
高影响因子
Applied Food Research
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2025
·
IF 6.2
Yan-fei Zheng,Qiang Zhang
This study optimized the ultrasound-assisted enzymatic extraction (UAEE) of polysaccharides from sugarcane leaves and evaluated their structural and antioxidant properties. Response surface methodology was used to optimize the extraction, achieving a high polysaccharide yield of 4.31 ± 0.08%, which significantly surpassed that of conventional methods. Purification yielded two distinct, low-molecular-weight polysaccharide fractions (P-1 at 15 kDa and P-2 at 9.6 kDa). Structural characterization revealed significant differences in monosaccharide composition; P-1 was rich in glucose and xylose, while P-2 exhibited greater structural diversity, including a high content of uronic acid. Both fractions demonstrated dose-dependent antioxidant activities. Notably, P-1 showed superior hydroxyl radical scavenging activity (IC₅₀ = 4.3 mg/mL) compared to P-2 (IC₅₀ = 5.2 mg/mL), whereas P-2 exhibited slightly better DPPH radical scavenging and ferric reducing power. These findings demonstrate that UAEE is a highly efficient method for producing low-molecular-weight, bioactive polysaccharides from sugarcane leaves, which show promise as natural antioxidants for further investigation in functional food and pharmaceutical applications.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Pin Gong,Xufeng Wang,Yewen Han,Hui Long,Wenjuan Yang,Fuxin Chen,Mengjiao Cui,Wenjun Zhai,Benzhong Zheng,Xuefeng Chen
This study devised optimal conditions to extract Eucommia ulmoides leaf (EUL) polysaccharides using a cellulase and pectinase composite enzyme system based on one-way experiments and response surface methodology. Crude EUL polysaccharides (EULPs) were extracted and purified using a DEAE chromatography column. The polysaccharides EUL-w, EUL1, EUL2, and EUL3 were obtained by elution with water, 0.1 mol/L NaCl, 0.2 mol/L NaCl, and 0.3 mol/L NaCl, respectively. The EUL-w fraction had the highest hypoglycemic activity based on its α-amylase and α-glucosidase activities. The preliminary structure of purified EUL-w1 was elucidated. In vitro hypoglycemic activity studies and metabolomics analyses suggested that EUL-w1 modulated glucose metabolism by mediating the AMPK/PI3K/Akt signaling pathway. Our findings provide novel insights and data support for the utilization of EULPs as an emerging food resource in functional foods.
研究领域:
药理学
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生物化学
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细胞生物学
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代谢组学
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DOI
PubMed
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
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2023
·
IF 2.9
Jin Chengyu,Wang Lingyun,Liu Xiaoying,Lu Yuanchao,Yu Ningxiang,Nie Xiaohua,Ye Qin,Meng Xianghe
Gardenia jasminoides Ellis, a representative for “homology of medicine and food”, can be used to produce pigment and edible oil. Here, aqueous enzymatic extraction (AEE) combined with puffing pre-treatment was explored to prepare oil from gardenia seeds. Both wet-heating puffing (WP) at 90 °C and dry-heating puffing (DP) at 1.0 MPa facilitated the release of free oil by AEE, resulting in the highest free oil yields (FOY) of 21.8% and 23.2% within 3 h, much higher than that of un-puffed group. Additionally, active crocin and geniposide were also completely released. The FOY obtained was much higher than mechanical pressing method (10.44%), and close to solvent extraction (25.45%). Microstructure analysis indicated that gardenia seeds expanded by dry-heating puffing (1.0 MPa) had a larger, rougher surface and porous structure than other groups. Overall, AEE coupled with puffing pre-treatment developed is an eco-friendly extraction technology with high efficiency that can be employed to oil preparation. Graphical abstract
研究领域:
食品科学
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生物技术
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化学工程
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Yuanchao Lu,Rongrong Hou,Shengxin Shao,Jialing Li,Ningxiang Yu,Xiaohua Nie,Xianghe Meng
Emulsification is the practical limitation of aqueous enzymatic extractions of Camellia oils. This study aimed to investigate the influence and demulsification mechanisms of isopropanol ultrasonic pretreatments and Ca 2+ additions on aqueous enzymatic extractions of Camellia oils. Combining isopropanol ultrasonic pretreatments with Ca 2+ flow additions obtained the highest free oil recovery (78.03 %) and lowest emulsion content (1.5 %). Results indicated that the superior demulsification performance originated from the decrease in emulsion stabilities and formations. First, demulsification pretreatments reduced the oil (14.69 %) and solid (13.21 %) fractions in emulsions to decrease the stability of as-formed emulsions. Meanwhile, isopropanol ultrasonic pretreatments extracted tea saponins (0.38 mg/mL) and polysaccharides (0.23 mg/mL), while Ca 2+ combined with protein isolates (5.82 mg/mL), tea saponins (7.48 mg/mL) and polysaccharides (0.78 mg/mL) to form precipitates and reduce emulsion formation. This work could promote the practical application of aqueous enzymatic extractions of Camellia oils and enlighten the rise of advanced demulsification pretreatments.
研究领域:
食品科学
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化学工程
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生物技术
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产品关联
DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2022
·
IF 9.336
Siting Wang,Zhiqing Liu,Shuang Zhao,Lin Zhang,Congfa Li,Sixin Liu
In order to obtain noni juice with high yield and good quality, the effect of combined extraction technique of enzymatic treatment (EZ) and ultrasonication (US) on the overall quality of noni juice was investigated. Moreover, the extraction performance of the EZ-US combined extraction technique was compared with that of EZ-based extraction and the US-based extraction. Response surface methodology (RSM) was designed to optimize the parameters of ultrasonic treatment, by taking consideration of the extraction efficiency, quality parameters and bioactive ingredients of noni juice. The results indicated that combined ultrasonic and enzymatic treatment achieved a synergistic effect on promoting the quality of noni juice. The maximum juice yield of 67.95 % was obtained under ultrasonication for 10 min at 600 W after enzymatic treatment (EZU). In addition, EZU-treated juice exhibited the highest contents of total phenolic and flavonoid, which were 148.19 ± 2.53 mg gallic acid/100 mL and 47.19 ± 1.22 mg rutin/100 mL, respectively, thus contributing to better antioxidant activity. Moreover, the EZU treatment significantly reduced the particle size of noni juice, and improved its suspension stability and rheological properties. FTIR results indicated that the treatments did not bring major changes in the chemical structure and the functional groups of compounds in noni juice. Therefore, EZU treatment can be successfully applied to the extraction of noni juice with better nutritional properties and overall quality.
研究领域:
食品科学
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生物技术
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化学工程
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农业工程
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产品关联
DOI
PubMed
高影响因子
Journal of Oleo Science
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2022
·
IF 1.628
Anning Liu,Huijuan Jing,Xiaojing Du,Chaoyang Ma,Hongxin Wang
In this study, the recovery of pecan nut kernel oil (PNKO) obtained by mechanical pressing (MP) was compared with that obtained by ultrasonic-assisted aqueous enzymatic extraction (UAAEE). At the same time, contents of substances with proven bioactivity, fatty acid compositions, physicochemical properties and antioxidant activities of PNKO were also assessed. Obviously, the oil yield obtained by UAAEE (71.32%) was higher than that obtained by MP (56.81%). Therefore, Plackett-Burman design (PBD), as well as Box-Behnken design (BBD), further optimized the UAAEE process, and the highest oil yield of 78.83% was acquired under the following conditions: incubation time of 2 h, the mixed enzyme (cell ulase+hemicellulase+pectinase+neutrase, w/w/w/w=1/1/1/1) concentration of 2.9%, liquid-solid ratio of 4 mL/g, pH of 4, particle size of 300 μm, ultrasonic time of 20 min, ultrasonic power of 432 W and reaction temperature of 53°C. The PNKO obtained by the two methods possessed similar fatty acid composition, but that obtained by UAAEE had comparatively low acid value and peroxide value. Moreover, rich content of total phenolics, squalene and phytosterols, as well as strong scavenging activities against DPPH and ABTS+ free radicals, were also contained in the PNKO obtained by UAAEE. These results demonstrated that UAAEE was an efficient method to acquire pecan nut kernel oil with excellent quality and high recovery.
研究领域:
食品科学
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生物技术
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化学工程
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农业工程
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2018
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IF 3.849
Kexin Hou,Xuebing Yang,Meili Bao,Fengli Chen,Hao Tian,Lei Yang
An efficient approach to isolate fruit oil from Idesia polycarpa using homogenate-circulating ultrasound-assisted aqueous enzymatic extraction (H-CUAEE) was developed. An enzyme cocktail comprising cellulase, hemicellulase, and pectinase proved to be the most effective in extracting oils. Nine potential variables that possibly affected the extraction yields – the liquid-solid ratio, incubation temperature, incubation time, amount of enzyme, homogenate time, ultrasound irradiation power, mark-space ratio of ultrasound irradiation, rate of agitation, and pH – were investigated using a Plackett–Burman design in order to screen out the significant variables. The liquid-solid ratio, incubation temperature, and incubation time were significant on a statistical basis and these three parameters were studied through a Box–Behnken design for the prediction of the best experimental conditions that maximize extraction yield of the fruit oil. A maximum fruit oil recovery of 79.36% with an error of 0.17% was achieved under the optimal extracting conditions: liquid-solid ratio of 7.13, the incubation time of 2.94 h, and the incubation temperature of 48.74 °C. The H-CUAEE process has expansive prospects for the preparation of oils from oil plants.
研究领域:
植物科学
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食品科学
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生物技术
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化学工程
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产品关联
DOI
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2023
·Vol. 249
·期 7
·1875-1885
·
IF 3.3
Lu Yuanchao,Hou Rongrong,Li Mengren,Yu Ningxiang,Huan Weiwei,Nie Xiaohua,Meng Xianghe
Camellia oleifera Abel is a traditional and high-quality oil source in China, characterized as polyphenols. This study evaluated how extraction methods impacted the phenolic profiles and antioxidant activities of Camellia oils. The contribution rates of phenolic profiles to antioxidant activities were investigated. HPLC and UPLC-Triple-TOF/MS demonstrated that phenolic content significantly varied with extraction methods while phenolic profiles were not. Herein, 15, 15, 17, 14, and 14 compounds were identified in mechanical pressing (MP), solvent extraction-hexane (SE-H), solvent extraction-ethanol (SE-E), aqueous enzymatic extraction (AEE), aqueous extraction (AE) oils, respectively. Results presented that SE-E oils were rich in phenolic acids (caffeic acid and p-hydroxybenzaldehyde), while AE oils were abundant in flavonoids (rutin and quercetin). Besides, the antioxidant activities of AEE and AE oils were significantly higher than other oils, originating from flavonoids (rutin and quercetin) and non-phenolics (rhododendron acid). Furthermore, Camellia oils from five extraction methods were remarkably classified by principal component analysis based on phenolic categories and antioxidant activities. It was assumed that low-polar and high-polar phenolic profiles were simultaneously transferred into SE-E oils, while only low-polar phenolic profiles (mainly flavonoids) were extracted into AE or AEE oils. The antioxidative origin of Camellia oils was ascribed to rutin, quercetin, and rhododendron acid. Our results provided an in-depth understanding of Camellia oils on phenolic profiles and the relationship between extraction methods and antioxidant activities.
研究领域:
食品科学
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分析化学
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植物化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）