BIOPROCESS AND BIOSYSTEMS ENGINEERING
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2024
·Vol. 47
·期 7
·1071-1080
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IF 3.5
Sun Liqun,Xu Chaozhong,Tong Shanshan,Gu Xiaoli
This study investigates the immobilization of cellulase on zeolitic imidazolate frameworks (ZIFs) by physical adsorption, specifically the ZIF-8-NH 2 and Fe 3 O 4 @ZIF-8-NH 2 , to enhance enzymatic hydrolysis efficiency. The immobilization process was thoroughly analyzed, including optimization of conditions and characterization of ZIF carriers and immobilized enzymes. The impacts on the catalytic activity of cellulase under various temperatures, pH levels, and storage conditions were examined. Additionally, the reusability of the immobilized enzyme was assessed. Results showed the cellulase immobilized on Fe 3 O 4 @ZIF-8-NH 2 exhibited a high loading capacity of 339.64 mg/g, surpassing previous studies. Its relative enzymatic activity was found to be 71.39%. Additionally, this immobilized enzyme system demonstrates robust reusability, retaining 68.42% of its initial activity even after 10 cycles. These findings underscore the potential of Fe 3 O 4 @ZIF-8-NH 2 as a highly efficient platform for cellulase immobilization, with promising implications for lignocellulosic biorefinery.
研究领域:
生物化学
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酶工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER SCIENCE
·
2023
·
IF 3.4
Difei Zhang,Ziyan Wang,Jieyu Gu,Yan Zhang,Jinghua Chen
Biocatalysis is playing an increasingly important role for chemical, pharmaceutical, and food industries, owing to its high efficiency, selectivity and environment-friendliness. However, it remains challenging to improve the catalytic activity of enzymes since the most frequently used strategies are costly and time-consuming. In the current study, a new type of glycogen-based dynamic nano-frameworks is prepared as additives for the direct activation of carbonic anhydrase ( bovine erythrocytes [bCA]). The boronic acid functionalization of the branched glycogen structures can provide N B coordination with bCA, leading to the conjugation and stabilization of the target enzyme. As a result, the glycogen with a 10% substitution degree ( Gly-PBA 10 ) showed a higher activation effect for bCA than that of glycogen with a 20% substitution degree ( Gly-PBA 20 ). Furthermore, the mechanism for interactions between the dynamic frameworks and bCA has been studied through combined techniques of dynamic light scattering, circular dichroism spectroscopy, and isothermal titration calorimetry. The results suggested different protein binding modes and stabilization abilities for the protein secondary structures from these two types of functionalized dynamic frameworks. This study provided new insights into the protein-polymer interactions that can be beneficial for various biochemical applications. Graphical
研究领域:
生物技术
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生物化学
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酶学
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纳米技术
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2015
·
IF 4.152
Shaojun Liang,Yan Duan,Zhen Xing,Haobo Han,Aijun Zhang,Li Li,Yan Yang,Quanshun Li
Chondroitin sulfate was chemically conjugated to PEI25K through Michael addition to construct a non-viral gene carrier CS-PEI, and then the carrier was employed in miR-34a delivery to achieve the inhibition of cell proliferation and migration, using prostate tumor cell PC-3 as a model. The nanoparticle from CS-PEI and miR-34a at a mass ratio of 10 was prepared with particle size and zeta potential of 170.7 nm and +42.2 mV, respectively. Flow cytometry and fluorescence microscopy revealed that CS-PEI could efficiently induce the cellular uptake of miR-34a in a CD44-mediated endocytosis manner. Through CS-PEI-mediated miR-34a transfection, obvious cell apoptosis was observed with early apoptotic cells of 47.49%, and meanwhile the activation of caspase-3, -8 and -9, and decreased expression level of Bcl-2 were detected. Moreover, wound healing assay showed that CS-PEI/miR-34a transfection could inhibit the cell migration. Overall, CS-PEI is potentially employed as a promising tumor-targeting system for miR-34a delivery in tumor gene therapy.
研究领域:
药学
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分子生物学
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癌症研究
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基因治疗
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产品关联
DOI
PubMed
高影响因子
DRUG DEVELOPMENT RESEARCH
·
2021
·Vol. 83
·期 3
·669-679
·
IF 4.36
Dan Luo,Xing Li,Yonghui Hou,Yu Hou,Jiyao Luan,Jiaxian Weng,Jiheng Zhan,Dingkun Lin
Spinal cord injury (SCI) leads to microvascular damage and the destruction of the blood spinal cord barrier (BSCB), which can progress into secondary injuries, such as apoptosis and necrosis of neurons and glia, culminating in permanent neurological deficits. BSCB restoration is the primary goal of SCI therapy, although very few drugs can repair damaged barrier structure and permeability. Sodium tanshinone IIA sulfonate (STS) is commonly used to treat cardiovascular disease. However, the therapeutic effects of STS on damaged BSCB during the early stage of SCI remain uncertain. Therefore, we exposed spinal cord microvascular endothelial cells to H 2 O 2 and treated them with different doses of STS. In addition to protecting the cells from H 2 O 2 -induced apoptosis, STS also reduced cellular permeability. In the in vivo model of SCI, STS reduced BSCB permeability, relieved tissue edema and hemorrhage, suppressed MMP activation and prevented the loss of tight junction and adherens junction proteins. Our findings indicate that STS treatment promotes SCI recovery, and should be investigated further as a drug candidate against traumatic SCI.
研究领域:
神经科学
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药理学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
MOLECULAR NEUROBIOLOGY
·
2016
·Vol. 55
·期 1
·421-434
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IF 5.397
Dai Chongshan,Ciccotosto Giuseppe D.,Cappai Roberto,Tang Shusheng,Li Daowen,Xie Sanlei,Xiao Xilong,Velkov Tony
Neurotoxicity is an unwanted side-effect seen in patients receiving therapy with the “last-line” polymyxin antibiotics. This is the first study to show that colistin-induced neurotoxicity in neuroblastoma-2a (N2a) cells gives rise to an inflammatory response involving the IL-1β/p-IκB-α/NF-κB pathway. Pretreatment with curcumin at 5, 10, and 20 μM for 2 h prior to colistin (200 μM) exposure for 24 h, produced an anti-inflammatory effect by significantly down-regulating the expression of the pro-inflammatory mediators cyclooxygenase-2 (COX-2), phosphorylation of the inhibitor of nuclear factor-kappa B (NF-κB) (p-IκB)-α, and concomitantly NF-κB levels. Moreover, curcumin significantly decreased intracellular reactive oxygen species (ROS) production and increased the activities of the anti-ROS enzymes superoxide dismutase, catalase, and the intracellular levels of glutathione. Curcumin pretreatment also protected the cells from colistin-induced mitochondrial dysfunction, caspase activation, and subsequent apoptosis. Overall, our findings demonstrate for the first time, a potential role for curcumin for treating polymyxin-induced neurotoxicity through the modulation of NF-κB signaling and its potent anti-oxidative and anti-apoptotic effects.
研究领域:
药理学
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神经科学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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