SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2024
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IF 4.3
Zhendong Fu,TongJia Zhang,Cong Chen,Xiaoyu Wang,Liping Wang
Nanozymes are nanoparticles with enzymatic activity, which are widely used in environmental and antibacterial research. Herein, we designed and synthesized novel amorphous nanozyme Cu-Im NPs with multiple enzyme-mimicking activities. Cu-Im NPs have the same active sites as natural laccase. In addition, the active center is similar to that of carbonic hydrolase, replacing the zinc ions with copper ions. Meanwhile, Cu-Im NPs also possess peroxidase-like activity. We reveal the multi-enzyme catalytic mechanisms of Cu-Im NPs. Notably, phosphate inhibits the laccase-like and peroxidase-like activities of Cu-Im NPs while activating their hydrolase activity. Based on these findings, we have developed a sensitive and selective method for detecting phosphate anions.
研究领域:
生物化学
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环境科学
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纳米医学
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
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2024
·Vol. 48
·期 11
·4886-4895
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IF 2.7
Haiyan Wang,Cheng Liu,Yue Zhao,Xinyu Luo,Pengjie Yin,Fuyou Du,Guangsheng Zeng
Nanozymes, as artificial enzymes, overcome the low catalytic efficiency, poor stability, and cost-effectiveness of previous artificial enzymes. In this work, vanadium carbide@vanadium oxide (V2C@VOx) nanosheets were synthesized by a one-pot hydrothermal method using V2C as a precursor and H2O2 as an oxidant. The prepared V2C@VOx nanosheets exhibited excellent multi-enzyme activity such as peroxidase-like activity, superoxide dismutase-like activity, and oxidase-like activity. The entire synthetic process of V2C@VOx nanosheets does not need any additional intercalation agent, strong acid, or base. Meanwhile, the synthesized method of nanozymes was simple, time-saving, and environmentally friendly. In particular, based on the multi-enzyme activity of V2C@VOx nanosheets, the nanozymes catalyzed H2O2 and dissolved O2 to oxidize 3′,3,5,5′-tetramethylbenzidine (TMB). Additionally, glucose reacted with glucose oxidase (GOx) to form H2O2 under water bath conditions. Therefore, the colorimetric sensing strategy of glucose was constructed based on the multi-enzyme activity of V2C@VOx nanosheets and the enzymatic reaction of glucose. The detection limit of the sensor was as low as 0.08 μM. Furthermore, the colorimetric method was successfully used to detect glucose in human serum. The sensor exhibited low cost, high stability, and easy construction. Overall, this work proposed a promising strategy based on novel vanadium-based nanozymes with excellent multi-enzyme activity for biomedical detection.
研究领域:
生物医学工程
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材料科学
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分析化学
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DOI
高影响因子
BIOCHEMISTRY
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2018
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IF 2.997
Qianwei Zhao,Wen Yang,Tong Qin,Zhen Huang
RNA is a key player in the cellular central dogma, including RNA transcription and protein synthesis. However, it is unknown whether RNA can directly interfere with DNA synthesis. Recently, we have found in vitro that while binding to DNA polymerase nonspecifically, RNA can transform DNA polymerase to display a moonlighting activity, dNTP phosphatase, in turn interfering with DNA synthesis. This phosphatase activity removes the γ-phosphate from dNTPs (generating dNDPs) and subsequently removes the β-phosphate from the formed dNDPs (generating dNMPs), confirmed by the noncleavable α,β-CH2-dGTP and β,γ-CH2-dGTP analogues. We also found that dGTP is the best substrate for the phosphatase, and the dNTP phosphatase activity is sensitive to the reaction medium. In addition, we have revealed that RNA can tune the activity of closely related proteins and give rise to new catalytic functions with subtle differences. Moreover, we have demonstrated in vitro that at the lower dNTP level, this phosphatase can directly inhibit DNA synthesis by dNTP depletion, though the phosphatase activity is 690-fold slower than the polymerase activity. Our observation in vitro suggests a plausible strategy for RNA to directly interfere with DNA polymerase and DNA synthesis in vivo.
研究领域:
分子生物学
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生物化学
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DOI
PubMed
高影响因子
ACS Applied Nano Materials
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2025
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IF 5.5
Huihui Su,Ruobo Chen,Yi Fan,Kaixin Liu,Li Fu,Li Jia
Lactose serves as a crucial biomarker for assessing the quality and safety of milk. Therefore, the precise measurement of the lactose content is of paramount importance. Herein, we demonstrate that vanadium pentoxide (V2O5) nanobelts exhibit lactose oxidase-like activity. Additionally, a colorimetric biosensor for lactose detection was developed by leveraging a cascade reaction mechanism of V2O5 nanobelts possessing lactose oxidase-like and peroxidase-like activities. The sensor shows a linear response from 0.1 to 1.0 mM lactose, with a detection limit of 0.04 mM, and demonstrates accuracy and reliability in milk analysis. The accuracy of the proposed colorimetric method was verified by capillary electrophoresis in five milk samples. A self-designed portable device incorporating this technology serves to enhance its potential for practical application, enabling the precise and convenient detection of lactose levels in milk. This development not only simplifies the measurement process but also broadens the scope of its use in various settings, including on-site testing and quality control.
研究领域:
生物化学
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食品科学
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分析化学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2022
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IF 14.224
Jinghui Wang,Renliang Huang,Wei Qi,Rongxin Su,Zhimin He
Despite the rapid development of nanozyme , it is still challenging to develop multifunctional nanozyme with high oxidase-like activity. In this work, a novel type of amorphous imidazole-Cu nanozyme (I-Cu) with high laccase- and catecholase-like activity was prepared by the water induced precipitation of Cu 2+ and imidazole to mimick the N-Cu coordinated environment in their active site. I-Cu shows similar K m but 3.7-fold higher V max than laccase at the same mass concentration. The possible catalytic mechanism of I-Cu nanozyme, which is composed of substrate binding, substrate oxidation , oxygen binding and oxygen reduction, is proposed. In addition, it also displays high stability under various pH, temperature, long-term storage and high salinity . The oxidation efficiency of I-Cu nanozyme for environmental phenolic pollutants (2,4-dichlorophenol) is 91.8% at 10 h, which is higher than that of laccase (68.8% at 10 h). Furthermore, the colorimetric and smart phone detection of dopamine by I-Cu nanozyme is developed with the detection limit of 0.412 μM. Therefore, we expect this amorphous nanozyme is promising in the various application of bioremediation and biosensor .
研究领域:
生物化学
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环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2023
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IF 15.1
Xiaomei Dai,Qingqing Xu,Yu Li,Lele Yang,Yongjie Zhang,Xiaojun Liu,Feng Gao
Skin wound healing remains a key challenge owing to its vulnerability to pathogen infection, oxidative stress and persistent inflammatory response. Herein, a salen-manganese complex (EUK)-based nanozyme was designed to simultaneously circumvent these barriers. The nanozyme was prepared by the amidation reaction between degradable chito-oligosaccharide (COS) and EUK. Positive charged COS and metallic compounds (EUK) could allow the nanozyme (COS-EUK) synergistic antibacterial activity. Due to the protection and synergistic catalytic enhancement effect provided by COS, superoxide (SOD)- and catalase (CAT)-like capability of COS-EUK was significantly improved compared with EUK. The nanozyme could eradicate not only O 2 − but also the generated H 2 O 2 , further supplying O 2 to promote fiber cell proliferation. Moreover, COS-EUK could prevent macrophages from transforming into classically activated macrophages (M1 phenotype), and endow them anti-inflammatory effects. COS-EUK can accelerate bacteria-infected skin wound healing. Thus, COS-EUK provides a promising strategy for bacteria-infected wound healing with great potential in clinical infection.
研究领域:
生物医学工程
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药理学
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材料科学
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DOI
高影响因子
Journal of Environmental Chemical Engineering
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2024
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IF 7.4
Guobao Ning,Huan Liang,Linjiang Guo,Xiaokang Lu,Lin Xiao,Tianle Qi,Hui Zhao,Can-Peng Li
The application of single-atom nanozymes in the removal of organic dye pollution by advanced oxidation process (AOPs) have been widely concerned. However, the application of single-atom nanozymes in AOPs is rarely reported. In this work, a kind of Fe-and nitrogen-codoped carbon (Fe-N-C) with peroxidase-like and laccase-like activities was prepared by calcining Fe-doped MOF, then the chiral peptides were introduced into single-atom nanozymes, and chiral single-atom nanozymes (L-Au@FeNC) were successfully prepared. The L-Au@FeNC can effectively activate PMS to degrade different kinds of organic dyes and also shows high cycle stability. In addition, the results of quenching experiment and electron paramagnetic resonance (EPR) test show that 1 O 2 produced during PMS activation plays an important role in the degradation of organic dyes, and O 2 ·- , ·OH, and SO 4 ·- are also produced during PMS activation, suggesting that the degradation of selected organic dyes include radical and non-radical pathways. It should be noted that L-Au@FeNC showed higher activity than D-Au@FeNC in enzyme-like activity (laccase-like and peroxidase-like) experiment and organic dye degradation, the results of molecular docking simulation indicated that it may be due to the better binding force of L peptide to the substrate of enzyme-like reaction and organic dye than D peptide. This chiral single-atom nanozymes provide a new idea for the application of organic dye degradation and enzyme-like catalysis design.
研究领域:
环境科学
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生物化学
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材料科学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2017
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IF 3.034
Juan He,Fujian Xu,Jing Hu,Shanling Wang,Xiandeng Hou,Zhou Long
A triazine-based covalent organic framework, CTF-1, for the first time, was found to possess both intrinsic peroxidase-like activity and oxidase-like activity and be capable to catalyze the oxidation of chromogenic substrates with or without H 2 O 2 with high efficiency as a robust catalyst. Related oxidization mechanism was thoroughly studied, and the according catalytic properties of CTF-1 was also evaluated. What's more, a facile and effective colorimetric assay was established to detect biothiols without using any oxidizing agents. Compared to natural enzymes and other reported artificial enzymes, CTF-1 has also demonstrated the advantages including low cost, easy preparation, good stability, and high catalytic activity, which made it a very promising enzyme-mimetic alternative in potential applications in biosensors for the future.
研究领域:
生物化学
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材料科学
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分析化学
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产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Jian-Qing Liu,Ye-Bin Feng,Yin-Feng Xiao,Lu-Fang Chen,You-Xia Huang,Shui-Sen Lin,Yin Zhang,Qiu-Xia Xu,Shao-Bin He,Wei Chen
Glucose isomerase (GI), also known as D-xylose isomerase, plays a crucial role in converting glucose to fructose, widely applied in the food and agricultural industries. However, current GI activity detection methods suffer from limited sensitivity and operational complexity. Herein, we propose a novel detection strategy by coupling GI-catalyzed isomerization with a self-cascade copper oxide (CuO) nanozyme system. This cascade leverages the dual fructose oxidase-like and peroxidase-like activities of CuO nanozymes to achieve efficient signal amplification. The method offers a broad linear range (0.3–9.375 U/mL) and a low detection limit (0.11 U/mL), outperforming existing detection techniques. Spiking recovery experiments were performed on the glucose isomerase extract derived from Escherichia coli cultures, the culture medium, and commercial glucose isomerase preparations, yielding recoveries ranging from 86.22 % to 107.34 %. Given its high sensitivity, simplicity, and practicality, this method shows great promise for screening GI-producing bacterial strains and for the detection of GI activity in various applications.
研究领域:
纳米生物技术
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酶工程
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生物传感器
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食品生物技术
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2017
·
IF 4.529
Le-Le Shao,Xiao-Ling Wang,Kai Chen,Xiao-Wu Dong,Li-Min Kong,De-Yin Zhao,Robert C. Hider,Tao Zhou
A range of hydroxypyridinone derivatives were synthesized starting from kojic acid. Among them, 10 and 11 were found to possess the strongest inhibitory effect on monophenolase activity of mushroom tyrosinase, having IC 50 values of 2.04 and 1.60 μM, respectively. The IC 50 values of 10 and 11 for the inhibition of diphenolase activity of mushroom tyrosinase were determined as 13.89 and 7.99 μM, respectively. Investigation of the inhibitory mechanism of these two compounds indicated that the inhibition was reversible and of a competitive-uncompetitive mixed type. The K I and K IS values of 10 were determined to be 24.84 and 32.54 μM, respectively, and the corresponding values for 11 being 18.07 and 21.34 μM, respectively. The effect of 11 on the browning process of fresh-cut apples was evaluated by measuring the color change and browning index. The results indicated that 11 had a significant effect on controlling the browning of fresh-cut apple slices.
研究领域:
食品科学
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生物学
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植物学
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2025
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IF 5.1
Walaa Al-Mathagi,Shuairu Zhu,Hongwei Xu,Juan Zhou,Mei Yang,Binwu Ying
Nanozymes with multi-enzymatic activity in biomedical fields have gained significant attention. However, the effects of metal-doping elements on the structure–activity relationship of many nanomaterials remain insufficiently understood. Herein, we selected NiFe-LDH as the base material to systematically investigate how varying Mn doping ratios and specific Mn doping sites within the NiFe-LDH lattice influences peroxidase (POD), oxidase (OXD), and catalase (CAT) activities. Notably, substitution of Mn at both the Ni and Fe sites in NiFe-LDH (designated as NiFeMn-20%-C) led to markedly improved multi-enzymatic activities compared to Mn substitution at only the Fe site (NiFeMn-20%). To elucidate the mechanisms underlying these enhancements, density functional theory calculations are employed to assess the Gibbs free energies associated with various enzymatic reaction intermediates at the two classes of Mn active sites. These results indicate that the Mn sites in NiFeMn-20%-C are more favorable for promoting the redox conversion of H2O2 and O2 than those in NiFeMn-20%. Our results underscore the critical importance of carefully selecting appropriate metal substitution sites in the design of LDH-based materials to achieve enhanced multi-enzymatic activity.
研究领域:
纳米技术
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材料科学
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生物化学
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生物医学工程
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催化
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 12
·4629-4635
·
IF 4.58
Yang Haiguan,Yang Ruitao,Zhang Peng,Qin Yuemei,Chen Tao,Ye Fanggui
A bimetallic metal-organic framework of the type MOF(Co/2Fe), synthesized by a hydrothermal method, is demonstrated to exhibit peroxidase-like activity and oxidase-like activity. The material catalyzes the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) to produce a blue product both in the absence and presence of H 2 O 2 . Its catalytic activity strongly depends on temperature and the solution pH value. With its dual enzyme mimetic activity, MOF(Co/2Fe) has the advantages of low cost, good stability, ease of preparation, and attractive Michaelis-Menten behavior. The mechanisms and kinetics of the pseudo-enzymatic activity were studied in some detail. A colorimetric method was developed for determination of H 2 O 2 that is based on the peroxidase-like activity of MOF(Co/2Fe). Under the optimal conditions, the absorbance of oxidized TMB at 652 nm increases linearly in the 10 to 100 μM H 2 O 2 concentration range, with a 5 μM limit of detection.
A bimetallic metal-organic framework of the type MOF(Co/2Fe) exhibits peroxidase-like activity and oxidase-like activity. The material catalyzes the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) to produce a blue product in the presence and absence of H 2 O 2 .
研究领域:
材料科学
·
生物化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）