Journal of Macromolecular Science Part B-Physics
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2024
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IF 1.2
Jie Jiang,Jianjun Fang,Wenjuan Liu,Meimei Guo,Zhongjia Lu,Heng Tang,Yi Lan,Zhihua Zhou
Composite porous microcarriers based on demineralized bone extracellular matrix (DBECM) and chitosan (Cs) were prepared using a combined emulsion and freeze-drying method. The effects of digestion by pepsin on the DBECM properties were investigated. The morphologies and porous structure of the DBECM/Cs porous microcarriers were observed by using a scanning electron microscope (SEM). The cytocompatibility of the DBECM/Cs porous microcarriers were studied by using cell counting kit-8 (CCK-8) and calcein acetoxymethyl ester/propidium iodide (CAM/PI). DBECM was digested into small pieces with a loose porous sponge structure and the digested DBECM showed an amorphous state. The resultant DBECM/Cs porous microcarriers showed a spherical shape and an interconnective porous structure. The CCK-8 and CAM/PI analyses indicated that the DBECM/Cs porous microcarriers were non-cytotoxic and showed good biocompatibility, providing a more favorable growth environment for cells.
研究领域:
生物医学工程
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材料科学
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组织工程
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DOI
高影响因子
Frontiers in Sustainable Food Systems
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2024
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IF 3.7
Yang Ruoqing,Fei Zhuocheng,Wang Luyi,Tang Haohao,Sun Wanqiang,Li Mei,Lei Qingzi,Chen Jian,Guan Xin
Fibroblasts are important components of animal tissues such as muscle and skin, as they are the major producers of various matrix proteins. Matrix proteins such as collagen play an important role in meat products by providing unique nutrition, texture, and flavor. Cultured meat is an innovative meat alternative produced by culturing animal cells, but currently, relatively few studies have been conducted using fibroblasts as seed cells for cultured meat manufacturing. In this work, we first developed an innovative digestion-friction method for isolating fibroblasts from porcine skin efficiently and cost-effectively. After optimizing the enzymatic digestion and physical friction conditions, 2.39 ± 0.28 × 105 fibroblasts were obtained from 1 cm2 of porcine skin tissue, which was about 9 times higher than the conventional tissue explant method. In addition, we identified an edible bio-ink composed of gelatin and chitosan that has good printing properties and supports fibroblast adhesion and growth. Furthermore, we fabricated fibroblast-based cultured meat by 3D printing with an initial cell density of 1.0 × 107 mL−1 and evaluated its texture and nutritional properties. This work provides valuable insights and references for introducing fibroblasts into the production of cultured meat that is more comparable to structured animal meat.
研究领域:
组织工程
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生物打印
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细胞培养
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食品科学
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生物技术
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产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
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2025
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IF 7.3
Yequan Sheng,Xuan Wu,Jing Qi,Guijiang Wang,Cheng Dong,Xueqing Liu,Yanqing Wang,Chenhuan Lai,Xin Tan
This study presents a novel, cost-effective strategy employing nucleophilic polymers as direct additives during dilute acid (DA) pretreatment (2% H2SO4, 170–180 °C) to enhance the bioconversion efficiency of various lignocellulosic biomasses, including poplar (PP), mulberry branches (MB), and corn stover (CS). Screening among nine nucleophilic polymers identified carboxymethyl chitosan (CMS) and gelatin (GE) as exceptional dual-function enhancers to improve both prehydrolysate fermentability and enzymatic hydrolysis yield of pretreated substrates. The addition of CMS in pretreatment significantly detoxified prehydrolysates by reacting with key carbonyl inhibitors (reducing furfural by 19.8–38.6%, 5-hydroxymethylfurfural (HMF) by 3.8–59.1%, vanillin by 16.1–34.2%, syringaldehyde by 15.8–48.2% vs DA prehydrolysate controls). The addition of CMS dramatically boosted the fermentability of prehydrolysates with Saccharomyces cerevisiae, elevating ethanol yields to 7.33 (MB), 6.86 (CS), and 8.36 g/L (PP) from 6.28, 1.66, and 6.95 g/L in DA controls, respectively. Simultaneously, GE addition profoundly improved the enzyme digestibility of pretreated materials, increasing the 72-h enzymatic hydrolysis yields by 64.82 to 49.94%, 38.71 to 97.83%, and 44.26 to 55.15% for MB, CS, and PP, respectively. The findings from XPS and chemical compositional analyses indicated that the better enzyme digestibility might be related to in situ lignin modification with GE assistance. Crucially, both polymers demonstrated consistent efficacy across diverse feedstocks, with CMS also mildly aiding digestibility and GE contributing to the detoxification of pretreated biomasses. This direct addition approach with nucleophilic polymers enhanced enzyme digestibility and fermentation of pretreated materials concurrently without costly post-pretreatment steps, offering a significant advancement for efficient biofuel production.
研究领域:
生物质能源
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生物化学工程
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绿色化学
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工业生物技术
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可再生能源
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2017
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IF 2.603
Mingyu Zhang,Gong Li,Yi Zhang,Jingwu Kang
The antithrombin III (ATIII)-binding site, which contains a special 3- O -sulfated, N -sulfated glucosamine residue with or without 6- O -sulfation, is mainly responsible for the anticoagulant activity of heparin. Undergoing the chemical depolymerization process, the preservation of the ATIII-binding site in low molecular weight heparins (LMWHs) are varied leading to the fluctuation of the anticoagulant activity. Herein we report a capillary electrophoresis (CE) method in combination with heparinase digestion and affinity chromatography for the measurement of molar percentage of ATIII-binding site of LMWHs. After exhaustively digesting LMWHs with the mixture of heparinase I, II and III, almost all the resulting oligosaccharide building blocks, including the three 3- O -sulfated tetrasaccharides derived from the ATIII-binding site, were resolved by CE separation. The peak area of each building block permits quantification of the molar percentage of the ATIII-binding site. The peaks corresponding to the 3- O -sulfated tetrasaccharides were assigned based on the linear relationship between the electrophoretic mobilities of the oligosaccharides and their charge to mass ratios. The peak assignment was further confirmed by analysis of the high ATIII affinity fractions, which contains much high 3- O -sulfated tetrasaccharides. With the method, the molar percentage of the ATIII-binding site of enoxaparin from different batches and different manufactures were measured and compared. It was demonstrated that the CE method provides more precise data for assessing the anti-FXa activity than that of the biochemical assay method.
研究领域:
药物化学
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分析化学
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血液学
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产品关联
DOI
PubMed
高影响因子
Current Protocols
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2025
·Vol. 5
·期 7
·e70179
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IF 2.2
Bingyan Yuan,Jingjun Hong
In eukaryotic cells, nucleosomes are octameric structures composed of DNA and histones, and their tandem arrangement ultimately forms chromosomes. Nucleosome assembly is governed in vivo by a series of molecular mechanisms, particularly histone chaperones such as Swr1, which is indispensable for H2A.Z-nucleosome formation. The assessment of nucleosome assembly using micrococcal nuclease (MNase) digestion has been widely accepted. We describe a simple and reproducible protocol for analyzing the chaperone activity of SRCAP (Snf2-related CREBBP activator protein), the histone H2A.Z chaperone. This approach involves purifying a complex of H2A.Z, H2B, and SRCAP via fast protein liquid chromatography (FPLC) only, followed by its assembly with DNA-(H3.1-H4 A15C ) 2 . The formation of nucleosomes is then determined in vitro using electrophoretic mobility shift assay (EMSA) and MNase digestion. This approach can also be adapted to evaluate whether other histone chaperones or regulatory factors promote nucleosome assembly activity. © 2025 Wiley Periodicals LLC. Basic Protocol : In vitro nucleosome assembly facilitated by the histone chaperone SRCAP
研究领域:
分子生物学
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生物化学
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细胞生物学
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表观遗传学
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DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
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2020
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IF 7.632
Shuyu Shi,Xin Liu,Weiyi Li,Zhuo Li,Guoquan Tu,Baolin Deng,Chongxuan Liu
Sustainable development entails the revival of biopolymer-based membranes for water treatment with the expectation of addressing concerns relevant to the depletion of fossil-based resources and their negative impacts on the environment. However, it is challenging to tune the biodegradability of biopolymer-based membranes such that the filtration performance would be maintained during the service life while reducing the environmental footprint. This study established a conceptual framework of achieving tunable biodegradability by incorporating chitosan membranes with silver nanoparticles. In particular, the membranes were fabricated on the basis of dissolving chitosan in an aqueous urea/alkali solution, which enabled a green approach for forming various porous substructures via phase inversion. The effects of biodegradation on chitosan membranes were investigated in the context of correlating the filtration performance with the geometrical variations in the polymer network. A series of characterization experiments were implemented to corroborate that the degree of protecting the porous substructures against enzymatic digestion should be a function of the silver loading, whereas the service life would be dependent on the silver-leaching rate. Moreover, it was revealed that optimizing the coagulation conditions could be an effective way to control the silver release during the filtration process.
研究领域:
材料科学
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环境工程
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可持续发展
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纳米技术
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产品关联
DOI
高影响因子
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
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2023
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IF 2.1
Ke Shenghui,Feng Yiwei,Luo Liying,Qin Wanzhao,Liu Huayu,Nie Jingchong,Liang Beijiang,Ma Hongjie,Xie Mao,Li Jingyu,Niu Zhijie,Li Guojian,Tang Anzhou,Xia Wei,He Guangyao
A method for the in vitro isolation, purification, identification, and induced differentiation of satellite cells from adult tree shrew skeletal muscle was established. The mixed enzyme digestion method and differential adhesion method were used to obtain skeletal muscle satellite cells, which were identified and induced to differentiate to verify their pluripotency. The use of a mixture of collagenase II, hyaluronidase IV, and DNase I is an efficient method for isolating adult tree shrew skeletal muscle satellite cells. The P3 generation of cells had good morphology, rapid proliferation, high viability, and an “S”-shaped growth curve. Reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescence staining indicated that marker genes or proteins were expressed in skeletal muscle satellite cells. After myogenic differentiation was induced, multiple-nucleated myotubes were observed, and the MyHC protein was expressed. The expression of myogenic marker genes changed with the differentiation process. After the induction of adipogenic differentiation, orange-red lipid droplets were observed, and the expression of adipogenic marker genes increased gradually with the differentiation process. In summary, satellite cells from adult tree shrew skeletal muscle were successfully isolated using a mixed enzyme digestion method, and their potential for differentiation into myogenic and adipogenic cells was confirmed, laying a foundation for further in vitro study of tree shrew muscle damage.
研究领域:
细胞生物学
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肌肉生理学
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干细胞研究
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产品关联
DOI
PubMed
高影响因子
RENEWABLE ENERGY
·
2021
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IF 8.001
Qilin Zhang,Zongwei Guo,Xianhai Zeng,Bandaru Ramarao,Feng Xu
A sustainable and integrated biorefinery strategy was achieved by a facile biphasic system, ferric trichloride (FeCl 3 ) solution assisted by methyl isobutyl ketone (MIBK), yielding multiple products: furfural, sugars, and lignin nano-particles simultaneously. For the highest yield of furfural, the conditions were optimized resulting in 75.55% and over 69.38% of the yields from xylose and some typical biomasses respectively. Both the catalyst and MIBK can be recycled. Eucalyptus was lucubrated on the valorizations of three major-components with 75.18% of furfural yield, 97.54% of cellulase digestibility from the residue and lignin nano-particles. Meanwhile, the regularities of biomass degradation under this biphasic system were summarized as well as high digestibility which was resulted by hemicellulose and lignin removal in cooperation with the amount of the cellulase binding sites. Based on the proposed biorefinery strategy, 65.82% of the initial mass can be valorized into high-value end-products.
研究领域:
生物技术
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化学工程
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可持续化学
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生化工程
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2024
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IF 5.7
Wen Li,Chunli Gao,Yan Wang,Haiyue Zuo,Yangyang Bian,Cong Li,Shujuan Ma,Yehua Shen,Junjie Ou
Background The fabrication technique of capillary column is the key to the development and application of capillary liquid chromatography (cLC) to improve separation efficiency for analytes. The capillary monolithic column possessed three-dimensionally connected porous or channel structures. Unique porous structure endows excellent permeability and high performance in diverse fields, especially in separation. Thereinto, organic monolithic columns have attracted widespread attention due to their advantages of simple preparation and excellent biocompatibility . However, their separation selectivity needs to be further developed and regulated to apply the separation of more diverse samples. Results A novel polymeric monolithic column was prepared via thermally initiated in situ copolymerization of 2-methyladamantan-2-yl acrylate (MADA) with ditrimethylolpropane tetraacrylate (DTTA) in fused silica . The prepared poly(MADA- co -DTTA) monolith showed adjustable permeability, developed porous structure and high thermal stability . Consequently, it exhibited excellent separation capability of small molecules (alkylbenzenes and polycyclic aromatic hydrocarbons). Especially, when acetonitrile/water (60/40, v/v) was used as the mobile phase , the theoretical plate numbers reached 84,000 plates m −1 for butylbenzene at a linear velocity of 0.5 mm s −1 . Most importantly, the hydrophobicity of the poly(MADA- co -DTTA) monolithic column was regulated via host-guest interaction between adamantyl group and cucurbit [7]uril (CB[7]). Additionally, the poly(MADA- co -DTTA) monolith was further adopted for the analysis of the tryptic digest of proteins from HeLa by cLC-MS/MS. The 33,783 unique peptides and 5,299 proteins were identified on the monolith, which exhibited great separation ability for complex samples. Significance and novelty Due to abundant pore structure and good chemical properties, the poly(MADA- co -DTTA) monolithic column exhibited high performance for the separations of small molecules and biological sample. Meanwhile, owing to the existence of adamantyl-group, CB[7] was immobilized on the poly(MADA- co -DTTA) monolithic column to fabricate poly(MADA- co -DTTA)-CB[7] by host-guest interaction. It is possible to adjust the surface chemistry of the monolithic materials to accommodate more complex analytes.
研究领域:
分析化学
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色谱学
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生物分析
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材料科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
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IF 6.7
Xiao-Xia Zhou,Quanzhi Xiao,Kena Zhang,Yan Gao,Jie Zhang,Liping Fang,Bing Yan,Fangbai Li
The uptake, translocation, and transformation of engineered nanoparticles (ENPs) in plants present significant challenges due to the lack of effective determination methods. This is especially true for selenium nanoparticles (SeNPs), which hold promise for Se-biofortified agriculture and exhibit dynamic behaviors within plant system. Herein, we proposed a novel approach that incorporates enzymic digestion and membrane filtration to selectively extract SeNPs and dissolved Se from plant tissues, employing rice (Oryza sativa) plant as a model. Subsequently, the SeNPs retained on the membrane were quantified using inductively coupled plasma mass spectrometry (ICPMS), while the dissolved Se in the filtrate, including selenite (Se(IV)), selenate (Se(VI)), and seleno amino acid, were analyzed by liquid chromatography coupled with ICPMS (LC-ICPMS). Recoveries of 83.5–91.4% for SeNPs and 73.6–99.4% for dissolved Se at a spiking level of 8 μg/g in quality control samples were obtained. With the established method, it was discovered that SeNPs taken up by rice leaves can transform into Se (IV) and organic Se, and all the Se species could be translocated downward, but only Se (IV) and SeNPs could be excreted through the roots. These findings provide valuable insights into the fate of SeNPs in plants and their related biological responses.
研究领域:
环境科学
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植物科学
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分析化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Danya Lyu,Jing Li,Xiaowen Wang,Weiwei Guo,Erkang Wang
DNA-templated silver nanoclusters (DNA–Ag NCs) have shown great potential in various bioanalysis and bioimaging applications, owing to their facile synthesis and ultrasmall sizes and especially their programmable fluorescence emission depending on the sequences of DNA templates. However, the bioimaging applications of DNA–Ag NCs are severely limited by their poor stability in physiological environments and their poor cell permeability, resulting from the highly negatively charged DNA backbones. In this paper, cationic polyelectrolytes were used to modify fluorescent DNA–Ag NCs via electrostatic interactions between the positive polymer backbones and the negatively charged phosphate groups of the DNA strands. The fluorescence properties of a typical single-stranded-DNA (ssDNA)-templated Ag NC changed dramatically after incorporation with the cationic polyelectrolyte caused by conformational changes in the DNA, with a 3-fold fluorescence-emission enhancement of the Ag NCs observed after incorporation with a typical cationic polyelectrolyte, poly(diallyldimethylammonium chloride) (PDDA), whereas the fluorescence of double-stranded-DNA (dsDNA)-templated Ag NCs changed little after incorporation. Moreover, the modification with PDDA greatly prolonged the stability of both ssDNA- and dsDNA-stabilized Ag NCs against nuclease digestion and enhanced the cell uptake of the DNA–Ag NCs. Rapid cell imaging was demonstrated using NIH/3T3 cells as a model system.
研究领域:
纳米技术
·
生物化学
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生物医学工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
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2023
·
IF 6
Chenfei Han,Yu Xiao,Zhenjiang Liu,Daolin Du,Ming Li
To improve the performance of aptasensor, the coupling of novel material and strategy had been the hottest research with the promising prospects. In this study, a cascade amplifying aptasensor (CA-aptasensor), which had coupled the DNase I-assisted cyclic enzyme digestion and AgNPs@gel-enhanced fluorescence, had reflected the desired performance for positively correlated detecting OTA . For this purpose, the GO@PVP was used to adsorb and quench the free FAM-Apt, while the complex of OTA and FAM-Apt was digested by the DNase I. The released OTA allowed the free FAM-Apt to separate from the GO@PVP cyclically, thus the OTA level was amplified preliminarily. The AgNPs@gel could combine with the released FAM to form the metal enhanced fluorescence (MEF) effect, resulting in the cascade signal amplification. The OTA was detected at pg mL −1 level with an ultrasensitive LOD of 3.33 pg mL −1 and IC 50 of 13.6 pg mL −1 , which improved 90-fold and 728-fold than that of ELASA for same aptamer. The CA-aptasensor indicated high specificity, reliability and practicability. Meanwhile, the CA-aptasensor exhibited more highlights, including the efficient homogeneous system, low background signal and standardized aptamer recognizer. This study might provide a new approach for improving the performance of aptasensor, which could also guide the signaling cascade and positively correlated detection for other risk factors.
研究领域:
分析化学
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生物传感器
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纳米技术
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食品安全
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）