ACS Sustainable Chemistry & Engineering
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2023
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IF 8.4
Shuheng Wei,Bo Liu,Xiaonan Huang,Yongkang Xi,Shuxin Wang,Like Wang,Shouwei Yin,Xiaoquan Yang
Stimuli-responsive Pickering interfacial biocatalysis is a prominent topic in biphasic biocatalysis for its high efficiency and flexible tunability. Herein, we designed CO2/N2-responsive sodium caseinate (NaCas)–enzyme conjugates that acted as both catalytic sites and stabilizers to construct a responsive Pickering interfacial biocatalytic system. The conjugates were prepared by a one-step strategy in which amino groups reacted with carboxyl groups between NaCas and enzymes. In the meantime, NaCas, with a disordered structure, could act as a buffer in the microenvironment to improve enzyme stability in harsh environments. The emulsion system stabilized by horseradish peroxidase (HRP)–NaCas displayed a higher catalytic efficiency and conversion rate compared with the conventional two-phase system, and HRP–NaCas could be easily recycled at least five times by bubbling CO2 and N2. Furthermore, the coupled NaCas system was implemented in Candida antarctica lipase B (CaLB), which extended the excellent interfacial characteristics and application field of NaCas.
研究领域:
生物化学
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酶工程
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材料科学
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产品关联
DOI
高影响因子
ANALYST
·
2018
·Vol. 143
·期 20
·4907-4914
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IF 3.864
Yufeng Zhou,Yali Yu,Longlong Gao,Yifan Fei,Ting Ye,Qiusha Li,Xiaoshun Zhou,Ning Gan,Yong Shao
Efficient structuring of DNA by small molecules is very crucial in developing DNA-based novel switches with an ideal performance. In this work, we found that inverting only the polarity of the 3′ terminal guanine of the thrombin-binding aptamer (3iTBA) totally eradicates the original TBA G-quadruplex (G4) structure in K+. The unstructured 3iTBA can be further refolded upon specifically interacting with small molecules of natural isoquinoline alkaloids (IAs) due to their fruitful binding patterns with variant nucleic acid structures. We identified that 3iTBA can serve as a topology selector for planar IAs. Nitidine (NIT), owing to the planar aromatic ring and coplanar substituents, is the most efficient to restructure the 3iTBA random coil toward the anti-parallel G4 conformation. However, common metal ions can't realize this structuring. The topology selector competency of 3iTBA toward IAs’ planarity can be visualized using gold nanoparticles (AuNPs) as the chromogenic readout. Our work expands the G4 repertoire by exploring the polarity inversion regulation and provides a new approach to switch nucleic acid structures toward a small molecule structure-sensitive sensor.
研究领域:
生物化学
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分子生物学
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纳米技术
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分析化学
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产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
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2013
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IF 3.777
Chong Sun,Xiaobo Wang,Xujie Yang,Lei Xing,Bo Zhao,Xiaodi Yang,Chun Mao
In this paper, we reported a novel label-free electrochemical aptasensors for thrombin detection in whole blood using self-assembled multilayers with carboxymethyl-PEG-carboxymethyl (CM-PEG-CM) and thrombin-binding aptamer (TBA). In the sensing strategy, CM-PEG-CM and TBA were assembled on the electrode surface via covalent binding. In the presence of target, the TBA on the outermost layer of the self-assembled multilayer would catch the target on the electrode interface, which makes a barrier for electrons and inhibits the electro-transfer, resulting in the decreased DPV signals. Using this strategy, a wide detection range (1 pM–160 nM) for target thrombin was obtained, with a low detection limit of 1.56 × 10 −14 M. The control experiments were also carried out by using bull serum albumin (BSA) and lysozyme in the absence of thrombin. The results showed that the aptasensors had good specificity, stability and reproducibility to thrombin. Moreover, the aptasensors could be used for detection of thrombin in whole blood which could provide a promising platform for fabrication of aptamer based biosensors in clinical application.
研究领域:
生物医学工程
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分析化学
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分子诊断
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产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2024
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IF 11
Xitong Liu,Fengzhang Wang,Jirong Wang,Ting Liu,Chengying Zhao,Jinkai Zheng
Pectin and flavonoids are important components of citrus fruits, but the effects of flavonoid grafting on the emulsifying properties of citrus pectin remain unclear. In this study, a novel natural citrus flavonoid-pectin conjugate in citrus peel was identified through alkaline hydrolysis combined with ultra-high performance liquid chromatography. Subsequently, two major citrus flavonoids were enzymatically conjugated to citrus pectin at high grafting rates (hesperidin: 20.21%; naringin dihydrochalcone: 16.11%). The flavonoids increased pectin amphipathy while ensuring a tighter conformation and denser network structure. These structural changes reduced the interfacial tension (from 20.6 to 18.4 mN/m) and increased the adsorbed layer thickness (from 11.8 to 37.8 nm), facilitating rapid pectin anchoring at the oil-water interface. These led to smaller droplet size and better emulsion stability. Prepared flavonoid-pectin conjugates with high grafting rates exhibited better emulsifying properties than those with medium grafting rates; hesperidin-pectin conjugates were more effective than naringin dihydrochalcone-pectin conjugates. The prepared flavonoid-pectin conjugates aid in the structural identification of natural flavonoid-pectin conjugates; they also facilitate analyses of the properties of these conjugates and offer new insights into improving the functional properties of pectins used in the food industry.
研究领域:
食品科学
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化学
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生物技术
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产品关联
DOI
高影响因子
Inorganic Chemistry Frontiers
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2022
·Vol. 9
·期 14
·3470-3483
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IF 7.779
Xiao-Meng Liu,Zhe Li,Xin-Rui He,Rui-Ping Liu,Zhong-Ying Ma,Xin Qiao,Shu-Qing Wang,Jing-Yuan Xu
The combination of endocrine therapy and chemotherapy is an attractive approach for treating breast cancers. Aromatase inhibitors (AIs) are the first-line drugs for postmenopausal ER-positive breast cancer and adjuvant therapy drugs for early stages of breast cancer. In this paper, we employed the FDA-approved aromatase inhibitor aminoglutethimide (AG) to design and prepare a new class of multi-targeting Pt(IV) prodrugs (aminoplatins 1–3) by utilizing the targeting, estrogen-manipulating, and chemo-sensitizing effects of the aromatase inhibitor. Aminoplatin 3, as a representative, exhibited an enhanced anticancer activity up to 190-fold greater than the parent Pt(II) drug cisplatin, and 3 had less toxicity to normal cells, displaying higher anticancer efficacy and a superior therapeutic index compared to those of cisplatin. Moreover, aminoplatin 3 could enhance intracellular accumulation, induce remarkable DNA damage, inhibit migration and metastasis, and result in S phase cell cycle arrest and apoptosis in ER-positive MCF-7 cells. Besides, molecular docking results showed that 3 simultaneously occupies the heme iron-binding domain and the ASD-binding site of aromatase by a highly efficient dual-binding pattern to aromatase, which further displayed a striking anti-estrogenic effect by significant downregulation of CPY19A1 and ERα in ER-positive MCF-7 cells. Most strikingly, our in vivo anticancer evaluation data also provided evidence that 3 effectively inhibited tumor growth and attenuated kidney toxicity compared to cisplatin in MCF-7 xenografted BALB/c nude mice. Our results suggest that the anticancer activity of aminoplatins is mechanistically distinct from that of the conventional platinum drugs, and modifying platinum drugs with aromatase inhibitors may represent an improved modality for the treatment of advanced postmenopausal breast cancer.
研究领域:
肿瘤学
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药理学
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分子生物学
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癌症治疗
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DOI
高影响因子
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