Tao Feng,Zhongshan Hu,Yanzun Tong,Lingyun Yao,Haining Zhuang,Xiao Zhu,Shiqing Song,Jun Lu
Chicken liver is a main protein source to prepare attractant for dog food. However, animal proteins are costly. Seeking high quality and low-cost protein sources has been a goal for the industry. Mushroom Lentinus edodes (L. edodes) and Mealworm Tenebrio molitor (T. molitor) are novel protein sources, showing high potential as raw material of attractants. In this paper, chicken liver, L. edodes, and T. molitor were used as three different protein sources to prepare attractants. Their palatability to dogs were then compared. Firstly, the enzymatic hydrolysis process of three proteins was optimized, with a degree of hydrolysis of 54.82%, 36.10% and 30.14% for chicken liver, L. edodes , and T. molitor respectively. Secondly, volatile compounds of three attractants were identified by HS-SPME/GC-MS and SDE/GC-MS. Using OAV and PLRS method, it was found that bis(2-methyl-3-furyl) disulfide, indole, methional, 2-(methyl thio) phenol, γ -butyrolacton, furfuryl alcohol, acetic acid and isovaleraldehyde were the key components. Although both T. molitor and L. edodes attractant showed less palatability than that of chicken liver, they could be readily improved via adding key palatable volatile compounds. The ingestion rate of dog food with attractant showed a similar trend and was higher than that of food without attractant.
Plant-based milk alternatives are sustainable, hypoallergenic, and nutrient-rich, but challenges related to their lower bioavailability compared with animal-based milk still exist. In this study, we developed a multiple plant-based milk alternative using germinated soybeans and fermented cereals, and compared the protein digestible behaviors with commercial soy and bovine milk via in vitro gastrointestinal digestion. The multiple plant-based milk alternative possessed a higher level of essential amino acids and amino acid scores than the soy milk and a smaller percentage of low-molecular-weight peptides than the bovine milk. It displayed better protein-digestible responses with no apparent gastric coagulation. Moreover, the relatively larger particles in the multiple plant-based milk alternative had few effects on protein digestibility, with the highest proteolytic degree and a better free amino acid profile. The findings suggest that the multiple plant-based milk alternative presents higher protein digestibility behavior, and it could be a promising industrial plant-based product.
危险品化学品经营许可证（带存储）