BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Qihong Zhang,Zhaohui Lu,Chen Su,Zongmiao Feng,Hui Wang,Jingbo Yu,Weike Su
Traditional methods of enzymatic hydrolysis of cellulose to cellulose nanocrystals (CNCs) are limited due to the low enzymatic efficiency and large amount of waste liquid. The purpose of this study is to improve the yield and production efficiency of CNCs by enzymatic hydrolysis. A one-step mechano-enzymatic hydrolysis method was developed by utilizing the synergy of wet grinding and enzymatic hydrolysis reaction to efficiently prepare CNCs. Under the optimal reaction conditions, the maximum CNCs yield of 49.3% was achieved with higher thermal stability and crystallinity index of 76.7%. Mechano-enzymatic hydrolysis followed the first order pseudo-kinetics, and fractal kinetics demonstrated that mechanical force of rotation speed affected the fractal dimensions and binding ability between substrate and enzyme. This study provides an alternative method to prepare CNCs, which can significantly avoid the use of bulk water, improve the production efficiency of CNCs and thus lower the production cost.
研究领域:
材料科学
·
化学工程
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
Fermentation-Basel
·
2023
·Vol. 9
·期 9
·789
·
IF 3.7
William Rodrigues Alves,Thiago Alessandre da Silva,Arion Zandoná Filho,Luiz Pereira Ramos
This work aimed at producing lactic acid (LA) from sugarcane bagasse after steam explosion at 195 °C for 7.5 and 15 min. Enzymatic hydrolysis was carried out with Cellic CTec3 and Cellic HTec3 (Novozymes), whereas fermentation was performed withBacillus coagulansDSM2314. Water washing of pretreated solids before enzymatic hydrolysis improved both hydrolysis and fermentation yields. The presence of xylo-oligosaccharides (XOS) in substrate hydrolysates reduced hydrolysis efficiency, but their effect on fermentation was negligible. The presence of fermentation inhibitors in C5 streams was circumvented by adsorption on activated carbon powder with no detectable sugar losses. High carbohydrates-to-LA conversions (Yp/s) of 0.88 g·g−1were obtained from enzymatic hydrolysates of water-washed steam-exploded materials that were produced at 195 °C, in 7.5 min, and the use of centrifuged-but-never-washed pretreated solids decreased Yp/sby 16%. However, when the detoxified C5 stream was added at a 10% ratio, Yp/swas raised to 0.93 g·g−1for an LA productivity of 2.55 g·L−1·h−1. Doubling the pretreatment time caused a decrease in Yp/sto 0.78 g·g−1, but LA productivity was the highest (3.20 g·L−1·h−1). For pretreatment at 195 °C for 7.5 min, the elimination of water washing seemed feasible, but the use of longer pretreatment times made it mandatory to eliminate fermentation inhibitors.Keywords:sugarcane bagasse;steam explosion;enzymatic hydrolysis;fermentation;lactic acid
研究领域:
生物技术
·
化学工程
✓ 已验证
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2017
·
IF 5.651
Xiudong Zhang,Yuanyuan Bai,Xuefei Cao,Runcang Sun
Herein, an efficient biphasic pretreatment process was developed to improve the production of furfural (FF) and glucose from Eucalyptus . The influence of formic acid and NaCl on FF production from xylose in water and various biphasic systems was investigated. Results showed that the addition of formic acid and NaCl significantly promoted the FF yield, and the biphasic system of MIBK (methyl isobutyl ketone)/water exhibited the best performance for FF production. Then the Eucalyptus was pretreated in the MIBK/water system, and a maximum FF yield of 82.0% was achieved at 180 °C for 60 min. Surface of the pretreated Eucalyptus became relatively rough and loose, and its crystallinity index increased obviously due to the removal of hemicelluloses and lignin. The pretreated Eucalyptus samples showed much higher enzymatic hydrolysis rates (26.2–70.7%) than the raw Eucalyptus (14.5%).
研究领域:
生物化学
·
可再生资源工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2017
·
IF 5.651
Dan Sun,Aftab Alam,Yuanyuan Tu,Shiguang Zhou,Yanting Wang,Tao Xia,Jiangfeng Huang,Ying Li,Zahoor,Xiaoyang Wei,Bo Hao,Liangcai Peng
In this study, total ten Miscanthus accessions exhibited diverse cell wall compositions, leading to largely varied hexoses yields at 17%–40% (% cellulose) released from direct enzymatic hydrolysis of steam-exploded (SE) residues. Further supplied with 2% Tween-80 into the enzymatic digestion, the Mis7 accession showed the higher hexose yield by 14.8-fold than that of raw material, whereas the Mis10 had the highest hexoses yield at 77% among ten Miscanthus accessions. Significantly, this study identified four wall polymer features that negatively affect biomass saccharification as p < 0.05 or 0.01 in the SE residues, including cellulose DP, Xyl and Ara of hemicellulose, and S-monomer of lignin. Based on Simons’ stain, the SE porosity (defined by DY/DB) was examined to be the unique positive factor on biomass enzymatic digestion. Hence, this study provides the potential strategy to enhance biomass saccharification using optimal biomass process technology and related genetic breeding in Miscanthus and beyond.
研究领域:
植物学
·
生物能源
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2016
·
IF 4.052
Lei Liu,Ruifen Zhang,Yuanyuan Deng,Yan Zhang,Juan Xiao,Fei Huang,Wei Wen,Mingwei Zhang
In this study, rice bran was successively steamed with α-amylase, fermented with lactic acid bacteria, and hydrolyzed with complex enzymes. The changes in phenolic profiles and antioxidant activities of the corresponding aqueous solutions from three stages were investigated. Compared to the first stage, fermentation and complex enzyme hydrolysis significantly increased the total phenolics, total flavonoids, total FRAP and ORAC values by 59.2%, 56.6%, 73.6% and 45.4%, respectively. Twelve individual phenolics present in free or soluble conjugate forms were also analyzed during the processing. Ferulic acid was released in the highest amount among different phenolics followed by protocatechuic acid. Moreover, a major proportion of phenolics existed as soluble conjugates. The results showed that fermentation and complex enzyme hydrolysis enhanced total phenolics and antioxidant activities of aqueous solution from rice bran pretreated by steaming with α-amylase. This research could provide basis for the processing of rice bran beverage rich in phenolics.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMASS & BIOENERGY
·
2025
·
IF 5.8
Liyi Zhang,Guangyong Zeng,Jianquan Luo,Yinhua Wan,Benkun Qi
While the mechanism for extracting lignin from biomass using deep eutectic solvent (DES) pretreatment has been widely investigated, few studies have specifically identified the grafting of hydrogen bond donors from DES onto the lignin surface during pretreatment and examined the effect of lignin modification on enzymatic cellulose hydrolysis. This study combines choline chloride (CC) with lactic acid (LA), oxalic acid (OA) and glycerol (Gly) to treat lignin. Structural characterization confirmed the incorporation of LA and OA via esterification, and Gly via etherification, onto lignin surface. Enzymatic hydrolysis results indicated that CC-OA-treated lignin strongly inhibited hydrolysis, with glucose yields ranging from ∼30 % to ∼46 %. In contrast, CC-LA- and CC-Gly-treated lignin exhibited weaker inhibition, with glucose yields varying from ∼50 % to ∼59 % for the former and ∼47 %–∼60 % for the latter, compared to untreated lignin with glucose yields of 51.25 % and 38.95% at two loadings. Notably, a CC-Gly-treated lignin, with lower loading, resulted in glucose yields ranging from 57.02 % to 60.22 %, showing no inhibition on cellulose hydrolysis compared to the control without lignin addition (57.87 %). Physicochemical analyses revealed variations in water contact angle, surface charge, and total phenolic hydroxyl content among DES-treated lignin samples, affecting cellulase-lignin interactions—specifically hydrophobic, electrostatic, and hydrogen bonding. This study clarifies the relationship between DES composition, lignin modification, and enzymatic cellulose hydrolysis efficiency, providing valuable insights into the effects of DES-induced lignin modifications on cellulose saccharification and addressing a significant gap in the literature.
研究领域:
生物化学
·
可再生资源与环境工程
✓ 已验证
产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2025
·
IF 9
Fanyang Meng,Thomas L. Eberhardt,Yaru Li,Chen Xu,Hui Pan
A novel ternary deep eutectic solvent (TDES) composed of glycine hydrochloride, ethylene glycol, and p -toluenesulfonic acid was synthesized. The prepared TDES exhibited highly efficient capacity for the pretreatment of bamboo biomass ( Phyllostachys edulis ). About 95 % of xylan and 89 % of lignin could be removed from the bamboo feedstock after being pretreated with the TDES at 100 °C for 1 h. In addition, a nearly complete glucose yield of 98.7 % could be achieved, which was 9.4 times higher than that of the untreated bamboo. The pH value of the TDES is an important factor affecting the bond cleavage between lignin and carbohydrates. Additionally, the polarity facilitates selective dissolution of lignin via hydrogen bonding. Molecular dynamics simulations reveal an increase in hydrogen bond formation between TDES and lignin model compounds. The findings establish the TDES as a green, efficient method for bamboo biomass pretreatment, facilitating the conversion of this biomass resource into glucose for bioenergy applications.
研究领域:
生物化学
·
可再生能源
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Linlin Liu,Chengyu Jin,Yuanchao Lu,Ningxiang Yu,Xiaohua Nie,Qin Ye,Yuanxiang Jin,Xianghe Meng
In this study, a novel aqueous enzymatic extraction (AEE) was developed to prepare camellia oil enriched with polyphenols, and the potential mechanism of in situ phenolics enrichment was explored. Camellia seeds were first subjected to AEE in a 25 % isopropanol solution containing 0.15 M glucose for 4 h, followed by the introduction of α -glycosidase β -glycosidase, or pectinase, and further hydrolysis was performed for 1 to 4 h. A higher free oil yield (FOR, 86.98 %) was obtained. Meanwhile, the highest total phenolic content (TPC) in oil (95.72 ± 1.53 mg GAE/Kg oil) was detected in the 4-h β -glycosidase-treated group, approximately 4-fold that of the control group (20.00 ± 1.02 mg GAE/Kg oil). HPLC analysis of the hydrolysate revealed that typical glycosylated polyphenols declined in the aqueous phase, while the content of less polar polyphenols, such as kaempferol-3-O-sambubioside, rutin, and quercetin, in the oil phase increased. These findings suggest that hydrophilic KGRG and KXR were hydrolyzed into relatively hydrophobic deglycosylated phenolics, which migrated into the oil phase, accounting for the enrichment of phenolics in camellia oil by the novel AEE. Furthermore, the stability of phenolics-abundant camellia oil was evaluated with respect to physicochemical properties, phenolics content, and lipid accompaniments content under various conditions. Results showed that the phenolics and lipid accompaniments remained relatively stable under a nitrogen atmosphere and at 4 °C refrigeration. Hence, the in-situ phenolics enrichment technique developed has the potential to prepare functional camellia oil with high biological activity and stability on a large scale.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CyTA-Journal of Food
·
2021
·
IF 2.255
Feng Lv,Chenhui Zhong,Shuiqing Wu,Linghua Wang,Haiyan Wu,Jiang Sun
We assessed the changes in nutritional and putrescent components during enzymolysis of Acetes chinensis. Oligopeptide and astaxanthin both reached a maximum of 46.78 ± 1.03% and 14.71 ± 0.32 mg/kg at 6 h, respectively. The total free amino acid changed from 6.37 ± 0.08 g/100 g to 14.21 ± 0.17 g/100 g at 8 h. However, the volatile base nitrogen reached 143.50 ± 6.21 mg/100 g at 10 h. The total biogenic amine was first decreased and then increased to 6,324.00 ± 10.56 mg/kg. Moreover, the acid and peroxide values reached 4.93 ± 0.17 mg/g and 5.56 ± 0.23 mg/kg at 10 h, respectively. Therefore, although Acetes chinensis hydrolysate was rich in nutrients, the putrescent components, including volatile base nitrogen, biogenic amine, acid and peroxide values, were increased during enzymolysis. Collectively, the enzymatic hydrolysis process of Acetes chinensis should be appropriately regulated to reduce the contents of putrescent components.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
Foods
·
2021
·Vol. 10
·期 8
·1942
·
IF 4.35
Ye Dong,Wen Yan,Xiao-Di Zhang,Zhi-Yuan Dai,Yi-Qi Zhang
The development of an efficient pretreatment, prior to enzymatic hydrolysis, is a good strategy for the sustainable use of refractory fish byproducts. This study compared hydrothermal pretreatments at 159 °C for 2 min, followed by water extraction (steam explosion-assisted extraction, SE) and 121 °C for 70 min (hot-pressure extraction, HPE), for the recovery of proteins from fish backbones. The effect of enzymatic hydrolysis on the properties of the obtained fish bone protein (FBP) was also evaluated. The results demonstrated that FBP had high contents of protein (81.09–84.88 g/100 g) and hydroxyproline (70–82 residues/1000 residues). After hydrolysis with Flavourzyme, for 3 h, the FBP hydrolysates that were pretreated with SE (SFBP-H) exhibited a better degree of hydrolysis (DH) and nitrogen recovery (NR), and a higher level of umami taste free amino acids (151.50 mg/100 mL), compared with the HPE-treated samples. The obtained SFBP-H mainly distributed below 3000 Da and had strong scavenging effects on 1,1-diphenyl-2-picrylhydrazy (DPPH) (IC50= 4.24 mg/mL) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) (IC50= 1.93 mg/mL) radicals. Steam explosion-assisted extraction is a promising route for recovering proteins from native fish bone materials, and improving the flavor and antioxidant activity of the hydrolysates.Keywords:fish backbone;protein;steam explosion;hot-pressure extraction;enzymatic hydrolysis
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2014
·
IF 5.039
Yanjuan Zhang,Qian Li,Jianmei Su,Ye Lin,Zuqiang Huang,Yinghua Lu,Guosong Sun,Mei Yang,Aimin Huang,Huayu Hu,Yuanqin Zhu
A new technology for the pretreatment of natural cellulose was developed, which combined mechanical activation (MA) and metal salt treatments in a stirring ball mill. Different valent metal nitrates were used to investigate the changes in degree of polymerization (DP) and crystallinity index (CrI) of cellulose after MA + metal salt (MAMS) pretreatment, and Al(NO 3 ) 3 showed better pretreatment effect than NaNO 3 and Zn(NO 3 ) 2 . The destruction of morphological structure of cellulose was mainly resulted from intense ball milling, and the comparative studies on the changes of DP and crystal structure of MA and MA + Al(NO 3 ) 3 pretreated cellulose samples showed a synergistic interaction of MA and Al(NO 3 ) 3 treatments with more effective changes of structural characteristics of MA + Al(NO 3 ) 3 pretreated cellulose and substantial increase of reducing sugar yield in enzymatic hydrolysis of cellulose. In addition, the results indicated that the presence of Al(NO 3 ) 3 had significant enhancement for the enzymatic hydrolysis of cellulose.
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2020
·
IF 4.244
Xuelian Zhou,Jue Liu,Tianjiao Huang,Huiyang Bian,Ruibin Wang,Jiulong Sha,Hongqi Dai
Enhanced enzymatic saccharification of Miscanthus (MC) biomass, based on a novel benzenesulfonic acid (BA)-induced fractionation at mild condition as the pretreatment, gives a good example for the efficient transformation of energy crops into value-added products with minimal environmental impact. The results indicated that BA could facilitate the rapid and high solubilization of both lignin and hemicellulose, approximately 90.8 % of lignin and 75.5 % of hemicellulose were removed at 80 °C within 20 min. Consequently, the subsequent enzymatic hydrolysis efficiency was greatly enhanced to obtain an improved enzymatic digestibility with a high glucose yield of 97.9 %. Our study opens up an innovative avenue for the efficient valorization of the recalcitrant energy crops as MC.
研究领域:
生物能源
·
植物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）