BIOELECTROCHEMISTRY
·
2024
·
IF 4.8
Zhikai Wang,Yunpeng Li,Zhuanzhuan Shi,Xiaohai Wang,Chunxian Guo,Chang Ming Li,Xiaoshuai Wu
The main challenges (sluggish electron transfer , low energy density) hinder the future application of enzymatic biofuel cells (EBFCs), which urgent to take effective measures to solve these issues. In this work, a composite of Au nanoparticles decorated graphdiyne (AuNPs@GDY) is fabricated and employed as the carrier of enzyme (G6PDH), and a mechanism based on π-π interaction of electron transfer is proposed to understand bioelectrocatalysis processes. The results show that the AuNPs@GDY composite exhibits the highest current density among the three materials (GDY, AuNPs, and AuNPs@GDY), which is 3.4 times higher than that of GDY and 2.5 times higher than that of AuNPs. Furthermore, the results reveal that the AuNPs could increase the loading of enzymes and provide more active site for reaction, while GDY provides highly π-conjugated structure and unique sp/sp 2 -hybridized linkages interface. This work provides new insights to explore a theoretical basis for the development of more efficient bioelectrocatalytic systems.
研究领域:
材料科学
·
生物化学
·
能源科学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Jiacong Wu,Xinnan Ma,Ting He,Juan Han,Yan Zhu,Chunmei Li,Yun Wang
The structural limitation of the photocatalyst itself is the main reason for the difficulty in increasing the enzyme loading amount in the photo-enzyme coupling system. Hereon, a g-C 3 N 4 -based photocatalyst ACN 45 with high enzyme loading amount was synthesized. Abundant 5 nm channels match well with the size of horseradish peroxidase (HRP) molecules, which enables the loading amount to reach 6.3 times that of pure g-C 3 N 4 . Meanwhile, the microenvironment provided by channels of ACN 45 significantly improves the activity and stability of the immobilized enzyme, and reduces the leaching of the enzyme molecules inside it. The internal immobilization of HRP did not affect the absorption of visible light by the photocatalyst, and high loading amount of photo-enzyme coupling system enhance the photocatalytic degradation of BPA. The degradation rate reached 94 % within 1 h, which is 5.9 times that of pure g-C 3 N 4 . The research findings open avenues for designing a photo-enzyme coupling system with high enzyme loading and high stability through simple steps.
研究领域:
环境科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2013
·
IF 2.579
Qinghong Ai,Dong Yang,Yuanbing Li,Jiafu Shi,Xiaoli Wang,Zhongyi Jiang
In this study, titanate nanotubes (TNTs) with desirable biocompatibility and hydrophilicity have been synthesized by a facile and cost-effective alkaline hydrothermal method, and used to immobilize the enzyme. The characterization results reveal that the prepared TNTs have a regular tubular morphology with a length about 100–180 nm and an outer diameter about 10 nm, and a BET specific surface area of 305.4 m 2 g −1 . Catalase (CAT), as the model enzyme, was pre-modified by 3-(3,4-dihydroxyphenyl) propionic acid (3,4-diHPP) via 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) coupling chemistry, and then covalently immobilized on the TNTs surface by the chelation of catechol groups with Ti 4+ ions. It is found that TNTs exhibits excellent performances as the immobilized supporter of enzyme: the enzyme loading is as high as 820 mg g of support −1 ; the relative activity of immobilized enzyme is about 60% of that of free enzyme; the immobilized CAT demonstrates enhanced storage and recycling stability.
研究领域:
生物化学
·
纳米材料科学
·
酶工程
✓ 已验证
产品关联
DOI
高影响因子
Advanced Science
·
2025
·
IF 14.1
Lingling Fan,Weiliang Dong,Jianqi Lu,Yujia Peng,Bin Xie,Ping Wei,Min Jiang,Su Chen
Uncontrolled degradation of wound dressings may result in residues, causing several negative effects on wound healing, such as secondary damage, undesirable inflammation, and scar skin formation. Here, an available strategy associated with the synthesis of enzyme-loaded ( Burkholderia cepacia lipase, BCL) polycaprolactone (PCL) nanofiber scaffolds, aligning with wound healing effects is reported. These scaffolds are fabricated via fiber microfluidic electrospinning degradation-control technique. The obtained scaffolds exhibit tunable degradation rates, achieving complete degradation within 12–72-h cycles. The acidic degradation products are further elucidated and reveal the potential degradation mechanism. The acidic degradation products create an optimal microenvironment during the hemostasis and inflammation stages of wound healing. Notably, in vivo experiments demonstrate the enzyme-loaded scaffolds effectively promote angiogenesis, reduce inflammatory responses, mitigate collagen deposition, and regulate fibroblast differentiation. This promotes rapid wound healing with a remarkable scarless rate of over 99% by day 21. New guidelines for scar-free healing dressings are proposed, which carry out faster degradation without microplastics (MPs) and toxic byproducts before scar formation. These principles might provide valuable insights and promise for developing more effective wound dressings.
研究领域:
生物材料学
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组织工程
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再生医学
✓ 已验证
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2012
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IF 5.602
Minggang Zhao,Jingyun Huang,Yu Zhou,Qi Chen,Xinhua Pan,Haiping He,Zhizhen Ye
A novel method of fabricating a free probe type nanoscale biosensor is first demonstrated. A free probe type biosensor based on a single one-dimensional (1D) mesoporous ZnO/Chitosan inorganic–organic hybrid nanostructure is constructed. The as-prepared biosensor exhibited excellent biosensing performance and was stable in solution due to its inorganic–organic hybrid structure. The special mesoporous nanostructure with protuberances favors enzymes loading and enhances electrical communication efficiency. The feasibility of employing a single 1D nanostructure as a separate free nanoprobe for biosensing is demonstrated. This kind of free probe type biosensor will not only provides a new tool for micro-targets detection in microcell and enzymatic studies, but also has the potential to be inserted into single cell or other microorganism for biological studies.
研究领域:
材料科学
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生物传感
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2017
·Vol. 23
·期 58
·14614-14622
·
IF 5.317
Jing Du,Mei-Da Cao,Shu-Li Feng,Fang Su,Xiao-Jing Sang,Lan-Cui Zhang,Wan-Sheng You,Mei Yang,Zai-Ming Zhu
Graphical Pinning down catalysts : Two new Preyssler-type polyoxometalate-based coordination polymers were synthesized and applied to immobilize horseradish peroxidase (HRP). These polymers exhibited higher enzyme loading, required a shorter loading time, and exhibited excellent reusability (see figure). Enzyme immobilization is of increasing importance for biocatalysis, for which good supports are critical. Herein, two new Preyssler-type polyoxometalate (POM)-based coordination polymers, namely, {[Cu(H 2 biim) 2 ][{Cu(H 2 biim) 2 (μ-H 2 O)} 2 Cu(H 2 biim)(H 2 O) 2 ]H[({Cu(H 2 biim)(H 2 O) 2 } 0.5 ) 2 ((μ-C 3 HN 2 Cl 2 ){Cu(H 2 biim)} 2 ){Z(H 2 O)P 5 W 30 O 110 }] ⋅ x H 2 O} n ( 1 : Z=Na, x= 9; 2 : Z=Ag, x= 10; H 2 biim=2,2′-biimidazole) were designed and synthesized. Compounds 1 and 2 exhibit the same skeletons, which contain multiple Cu II complex fragments and penta-supported {ZP 5 W 30 } (Z=Na, Ag) clusters. They were first employed to immobilize horseradish peroxidase (HRP). Results show that compounds 1 and 2 are good supports for HRP immobilization, and exhibit higher enzyme loading, lower loading times, and excellent reusability. The immobilized HRP (HRP/ 1 or HRP/ 2 ) was further applied to detect H 2 O 2 , and good sensitivity, wide linear range, low detection limit, and fast response were achieved. This work shows that POM-based hybrid materials are a new kind of promising support for enzyme immobilization.
研究领域:
生物化学
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材料科学
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催化化学
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Tianran Zheng,Luan Yang,Meili Ding,Chen Huang,Jianfeng Yao
Metal-organic frameworks (MOFs) could serve as efficient matrixes to immobilize cellulase because of their high stability and porous morphology. Herein, the Zr-based MOFs (UiO-66 and UiO-66-NH 2 ) assisted 20 wt% high-solids hydrolysis of untreated corncob residues (CRs) at low enzyme loading was investigated. Glucan hydrolysis yields increased to 60.55% and 71.47% by separately adding 4 g/L UiO-66 and UiO-66-NH 2 at 5 FPU/g-glucan cellulase dosage. The maximum hydrolysis yield reached 90.01% at 10 FPU/g-glucan in the presence of 4 g/L UiO-66-NH 2 . Analysis of free protein concentration and cellulase activity suggested that MOFs effectively increased cellulase catalytic activity and stability, thus boosted CRs enzymatic hydrolysis efficiency. Additionally, UiO-66-NH 2 immobilization gave a high catalytic activity because of the abundant anchor sites of NH 2 groups. This research presents the promising future of MOFs’ application in lignocellulosic biomass bioconversion and other areas requiring immobilized enzymes.
研究领域:
生物技术
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材料科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2018
·
IF 3.057
Li Fu,Yong Huang,Wen Cai,Huaiwei Zhang,Jiehui Yang,Dihua Wu,Weitao Su
Improving the hydrophilicity of titania nanotubular arrays (TNAs) could promote their interfacial reaction rate when they interact under aqueous conditions. In this work, we proposed a simple hydrothermal method for Si and Sr doping. A significant hydrophilicity improvement could be observed in TNAs after the doping process. Therefore, Si@Sr/TNAs exhibited a superior urease loading and showed excellent chronocoulometric performance towards urea interaction.
研究领域:
材料科学
·
电化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2018
·Vol. 23
·期 1
·169-178
·
IF 2.509
Hui Yuchen,Ma Xiaoyan,Qu Fengjin
A high-efficiency, stable, and flexible glucose/oxygen enzymatic biofuel cell is fabricated based on three-dimensional gold-coated nickel foam. The gold-coated layer makes the skeleton of nickel foam rougher, enhancing the relative apparent surface area of nickel foam and decreasing the electron transfer resistance. With the gold-coated nickel foam as substrate, the bioanode and biocathode exhibit good electrocatalysis activity and stability. By assembling the bioanode and biocathode with agarose gel electrolyte, cellulose acetate membrane, and silicone rubber layer-by-layer, the flexible biofuel cell is obtained. As per the results, the three-dimensional gold-coated nickel foam enlarges the enzymatic loading density to 5.68 × 10 −7 mol cm −2 , increasing the short circuit current density to 9.04 mA cm −2 . The maximum power density of this flexible biofuel cell reaches 2.32 ± 0.07 mW cm −2 with excellent stability for retaining 84.6% of its performance after 60 days. Additionally, the flexible biofuel cell can be very stable in and after 1000 times of bending.
研究领域:
生物化学
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材料科学
·
能源技术
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Weipan Peng,Shenghui Li,Yating zhang,Qiuting Zhang,Wenna Li,Houyu Han,Dianming Zhou,Sheng Wang,Jin Chang,Xiaoqun Gong
Tumors progression is usually characterized by proliferation, migration, and angiogenesis , and it leads to uncontrolled tissue growth and blocks the mechanism of apoptosis. Therefore, targeted angiogenesis and anti-apoptosis is considered promising therapeutic strategy for cancer prevention and treatment. Herein, we report a versatile and intelligent MnO 2 -PEI-DNAzymes (MnPDs) nanodevice for self-sufficient anti-tumor angiogenesis and pro-apoptosis synergistic gene silencing, coupling multi-target bimodal in situ imaging. Intracellularly, the multifunctional charge-reversal MnPDs undergo reduction and lysis by the overexpressed GSH, actualizing enhanced MR imaging and sufficient DNAzyme cofactors in tumor tissue, and enabling miRNA-155 and telomerase biomarkers dual-fluorescence imaging. Under the precise multimodal imaging, the dual DNAzymes enable self-sufficient Early growth response-1 (EGR1) and Survivin (SUR) mRNAs synergistic silencing, effectively inhibiting tumor angiogenesis and anti-apoptotic. Considering that accurate and efficient therapy has become a trend in cancer therapy, our proposed self-sustaining multifunctional nanodevice is promising for broad clinical applications.
研究领域:
癌症生物学
·
纳米技术
·
基因治疗
·
分子成像
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2020
·
IF 2.627
Jing Wang,Yongqin Lv
Ultrathin polydopamine microcapsules with hierarchical structure and porosity were prepared for the immobilization of multienzymes using metal-organic framework (MOF) as the template. The multienzyme/MOF composite was first prepared using a “one-pot” co-precipitation approach via the coordination and self-assembly of zinc ions and 2-methylimidazole in the presence of enzymes. The obtained nanoparticles were then coated with polydopamine thin layer through the self-polymerization of dopamine under alkaline condition. The polydopamine microcapsules with an ultrathin shell thickness of ~ 48 nm were finally generated by removing the MOF template at acidic condition. Three enzymes were encapsulated in PDA microcapsules including carbonic anhydrase (CA), formate dehydrogenase (FateDH), and glutamate dehydrogenase (GDH). FateDH that catalyzed the main reaction of CO 2 reduction to formic acid retained 94.7% activity of equivalent free FateDH. Compared with free multienzymes, the immobilized ones embedded in PDA microcapsules exhibited 4.5-times higher of formate production and high catalytic efficiency with a co-factor-based formate yield of 342%.
研究领域:
化学工程
·
材料科学
·
生物化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Ruizhi Li,Wenrui Zhang,Fanxing Meng,Xinbo Li,Zongda Li,Yan Fang,Minwei Zhang
Herein, the hollow Prussian blue with ultra-small silver nanoparticle agents (Ag-HPB) was prepared by the coating-etching method by applying Prussian blue (PB) coating on Ag nanoparticles (Ag NPs) and diffusing Ag NPs into the PB framework. The flexible biosensing platform based on Ag-HPB nanocomposites incorporated the excellent electrical conductivity of Ag NPs and the superior enzyme loading capacity of the hollow structure, which significantly enhanced its sensing performance. Subsequently, take glucose oxidase (GOx) and acetylcholinesterase (AChE) as examples. The sensing platform displayed a good sensitive response to glucose (Glu) (24.37 μA mM −1 cm −2 ) and a considerable limit of detection (LOD) for trichlorfon (TCF) as 2.28 pg/mL while exhibiting high stability and good reproducibility. Moreover, it can be applied to monitor trichlorfon in apple samples. Promisingly, the Ag-HPB prepared by the coating-etching strategy provides a reliable strategy for further development of sensitive and flexible biosensing platforms with excellent electrical conductivity and high enzyme loading.
研究领域:
纳米技术
·
生物传感器
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）